Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 1,158,399 G→C intergenic (+1922/‑13361) RPPX_05120 → / → RPPX_05130 hypothetical protein/channel protein TolC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099741,158,3990GC88.1% 1303.1 / 126.7 506intergenic (+1922/‑13361)RPPX_05120/RPPX_05130hypothetical protein/channel protein TolC
Reads supporting (aligned to +/- strand):  ref base G (27/33);  new base C (210/234);  total (238/268)
Fisher's exact test for biased strand distribution p-value = 7.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 512 total aligned reads displayed.

CGGCAGCTTCACCGTCACCCTCGACCCGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGAGCGAACTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGTTGGCGCTACCGGCAGCTTCAGCATCACCCTCGACCC  >  CP009974/1158262‑1158546
                                                                                                                                         |                                                                                                                                                   
cGGCAGCTTCACCGTCACCCTCGACCCGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATc                                                                                                                                        <  1:3561267/151‑1 (MQ=2)
    aGCTTCCCCGTCACCCTCGACCCGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATg                                                                                                                                    <  1:4155927/151‑1 (MQ=9)
     gCTTCACCGTCACCCTCGACCCGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGc                                                                                                                                   <  1:1560716/151‑1 (MQ=12)
     gCTTCACCGTCACCCTCGACCCGCCTCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCGGCCGCACCGCCCGCGCTGGTGATCAACGc                                                                                                                                   >  2:5128408/1‑151 (MQ=11)
      cTTCACCGTCACCCTCGACCCGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGcc                                                                                                                                  <  1:2814236/151‑1 (MQ=12)
         cACCGTCACCCTCGACCCGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGAc                                                                                                                               >  1:2965529/1‑151 (MQ=17)
         cACCGTCACCCTCGACCCGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGAc                                                                                                                               <  1:4501217/151‑1 (MQ=14)
          aCCGTCACCCTCGACCCGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACg                                                                                                                              <  1:1929671/151‑1 (MQ=12)
           ccGTCACCCTCGACCCGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCCACgg                                                                                                                             >  2:1188569/1‑151 (MQ=9)
           ccGTCACCCTCGACCCGCCTCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGGACAGATCACCGCGCCGGACATCACCGCCCCCGCCGCCCCGACCGAGCTGGTGATCAATGCCGAggg                                                                                                                             >  2:2350047/1‑151 (MQ=11)
            cGTCACCCTCGACCCGCCTCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGc                                                                                                                            <  2:4002171/151‑1 (MQ=255)
              tCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCCGCCGCACCGCGCCAACTGGCGATCCATGCCCCCGGCAg                                                                                                                          >  2:3528475/1‑151 (MQ=11)
              gcACCGTCGACCCGCCTCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAg                                                                                                                          <  1:2271991/150‑1 (MQ=255)
                aCCCTCGGCCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGAGCGAACTGGTGATCAATGCCGACGGCAGCg                                                                                                                        <  2:1528016/151‑1 (MQ=255)
                aCCCTCGACCCGCCTCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCCCCCCCCGAGCTGGTGATCAATGCCGACGGCAGCg                                                                                                                        >  2:2504820/1‑151 (MQ=11)
                aCCCTCGACCCGCCTCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCCCCCGACATCACCGCCCCTGCCGCACCGACCCAGCTCGTGATCCATGCCCACGGCAGCg                                                                                                                        >  2:3448258/1‑151 (MQ=11)
                  ccTCGACCCGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTg                                                                                                                      <  2:704774/151‑1 (MQ=12)
                     cGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGAGCGAACTGGTGATCAATGCCGACGGCAGCGTGGTc                                                                                                                   <  1:2690082/151‑1 (MQ=255)
                     cGACCCGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTc                                                                                                                   <  1:3964645/151‑1 (MQ=12)
                      gACCCGCCTCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCa                                                                                                                  >  1:3031253/1‑151 (MQ=12)
                       aCCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGAGCGAACTGGTGATCAATGCCGACGGCAGCGTGGTCAc                                                                                                                 <  2:4608043/151‑1 (MQ=12)
                        cccGCCTCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCAcc                                                                                                                >  2:2207076/1‑151 (MQ=9)
                         ccggcccAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGAGCGAACTGGTGATCAATGCCGACGGCAGCGTGGTCACCg                                                                                                               <  1:4598944/145‑1 (MQ=12)
                         ccGCCTCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCg                                                                                                               <  2:1242982/151‑1 (MQ=9)
                          cGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCgg                                                                                                              <  1:5003094/151‑1 (MQ=12)
                           cgcacaagCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGc                                                                                                             <  1:2734846/144‑1 (MQ=11)
                            gcacaagCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATc                                                                                                                                        <  2:4874665/117‑1 (MQ=11)
                            gcacaagCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGcc                                                                                                            <  1:3356911/145‑1 (MQ=11)
                            ccTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGGGATCAATGCCGACGGCAGCGTGGTCACCGGcc                                                                                                            >  1:708867/1‑151 (MQ=9)
                            ccTCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGcc                                                                                                            >  2:1341012/1‑151 (MQ=9)
                              tCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGt                                                                                                          >  1:290731/1‑151 (MQ=18)
                               cAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGAGCGAACTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCgcg                                                                                                         >  2:1589731/1‑149 (MQ=14)
                               cAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGCTCACCGGCCgcc                                                                                                         >  2:4179310/1‑149 (MQ=255)
                                 ggCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGcc                                                                                                       <  1:4254040/151‑1 (MQ=21)
                                    cAACGGCGAACTGCTGGACGTGGTCGCCATCGATGAAGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGaa                                                                                                    <  1:497139/151‑1 (MQ=255)
                                      aCGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAAcc                                                                                                  <  1:2172104/151‑1 (MQ=255)
                                      aCGGCGAGCTGCTGGACGTGGCCGCCAGCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATGACCGCGCCCGTGATCTCCGCCCCTGCCTCACCGACTGAGCTCGTGATCAAGGCCGACGTCAGCGTGGTCGCCGGCCGTGCCGAAcc                                                                                                  >  2:849967/1‑151 (MQ=11)
                                      aCGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCAc                                                                                                                 >  2:3693699/1‑136 (MQ=255)
                                         gCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCggg                                                                                               <  2:4505616/151‑1 (MQ=18)
                                           gAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGgca                                                                                             <  2:4445785/151‑1 (MQ=255)
                                               tgctgGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCAc                                                                                         <  1:2291056/151‑1 (MQ=19)
                                                 ctgGACGTGGTCGCGAGCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCg                                                                                       <  2:432032/151‑1 (MQ=255)
                                                      cGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCCGCCCGCACCGATCACCGCGCCCGACACCACCGCCCCCGCCGCACCGCCCGCGCCGGCGCTCAATGCCCACGGCCCCGCCGCCACCCGCCGTGCCCAACCGGGCAGCACCGTCcgc                                                                                  >  1:4822964/1‑151 (MQ=2)
                                                       gTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTAcgcg                                                                                 >  1:4075891/1‑151 (MQ=21)
                                                         ggTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCCGGCAGCACCGTACGCGTg                                                                               >  2:3285298/1‑151 (MQ=255)
                                                           tCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCt                                                                             <  2:1492490/151‑1 (MQ=255)
                                                            cGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTg                                                                            <  1:4322229/151‑1 (MQ=255)
                                                            cGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTg                                                                            <  2:2811200/151‑1 (MQ=21)
                                                             gCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTgg                                                                           <  1:1801840/151‑1 (MQ=25)
                                                               cATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGgcc                                                                         <  2:1037988/151‑1 (MQ=255)
                                                               cATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGgcc                                                                         >  2:115988/1‑151 (MQ=255)
                                                                   gATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCACTGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGccgccg                                                                     >  2:532167/1‑151 (MQ=255)
                                                                   gATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGccgccg                                                                     <  1:837126/151‑1 (MQ=255)
                                                                      gACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACg                                                                  <  1:115988/151‑1 (MQ=255)
                                                                         ggcggcGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGca                                                               <  1:1971370/151‑1 (MQ=255)
                                                                           cggcgTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGcacc                                                             <  2:4245912/151‑1 (MQ=255)
                                                                               gTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCAGtg                                                         >  2:1751811/1‑151 (MQ=255)
                                                                                tttCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACtgt                                                        <  2:4665387/151‑1 (MQ=25)
                                                                                tttCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACtgt                                                        <  2:1703481/151‑1 (MQ=25)
                                                                                 ttCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACtgtg                                                       <  1:3863994/151‑1 (MQ=19)
                                                                                   ccTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCt                                                     <  1:1077931/151‑1 (MQ=255)
                                                                                     tcccTGCCCGCACAGCTCACCGCGCCCGACGTCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTgg                                                   <  2:3129987/148‑1 (MQ=11)
                                                                                       gCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGt                                                 <  1:3255313/151‑1 (MQ=255)
                                                                                       gCTGCCCGCACAGAGCACCGCGCCCGACCTCACCGCCCCTGCCGCACCGACCGAGCTGGGGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGt                                                 <  2:1962156/151‑1 (MQ=255)
                                                                                         tGCCCGCACAGATCCCCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAg                                               <  2:2462909/151‑1 (MQ=255)
                                                                                         tGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAg                                               >  2:1875689/1‑151 (MQ=255)
                                                                                            ccGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGt                                            <  2:2853548/151‑1 (MQ=19)
                                                                                             cGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTg                                           <  1:1858606/151‑1 (MQ=255)
                                                                                               cacaGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGt                                         <  1:4900414/151‑1 (MQ=21)
                                                                                                 caGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAg                                       >  2:1400594/1‑151 (MQ=255)
                                                                                                  aGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGt                                      >  2:3194200/1‑151 (MQ=255)
                                                                                                   gATCCCCGCGCCCAACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGtt                                     <  1:5209345/151‑1 (MQ=255)
                                                                                                   gATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGtt                                     <  1:3737789/151‑1 (MQ=255)
                                                                                                    aTCGCCGCGCCGGACATCAACGCCCCTGCCGCCCCGACCGAGCTGGTGATCGATGCCGACGGCGGCGTGGTCCCCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGTTg                                    <  1:2070450/151‑1 (MQ=11)
                                                                                                    aTCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGTTg                                    <  2:2924924/151‑1 (MQ=21)
                                                                                                     gcACCGCGCCCGACATCACCGCCCCTGGCGCACCGACCGAGCTGGGGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGTTgg                                   <  2:3800351/150‑1 (MQ=255)
                                                                                                       aCCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGTTGgcg                                 <  1:597433/151‑1 (MQ=255)
                                                                                                       aCCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGTTGgcg                                 >  2:3947062/1‑151 (MQ=255)
                                                                                                            gcCCGACATCACCGCCCCTGCCGCACCGCCCGGGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGTTGGCGCTAcc                            <  2:2105913/151‑1 (MQ=255)
                                                                                                            gcCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGTTGGCGCTAcc                            >  2:2726525/1‑151 (MQ=255)
                                                                                                               cGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGTTGGCGCTACCGGc                         <  2:256862/151‑1 (MQ=255)
                                                                                                               cGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGTTGGCGCTACCGGc                         >  1:153333/1‑151 (MQ=255)
                                                                                                                caCATCCCCGCCCCTACCGCACCGACCGAGCTGGTGATCCATGCCGACGGCAGCGTCGTCACCGGCCGTGCCCAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGTTGGCGCTACCGGCa                        <  1:3442161/150‑1 (MQ=11)
                                                                                                                 aCATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGTTGGCGCTACCGGCAg                       >  1:1322889/1‑151 (MQ=255)
                                                                                                                 aCATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGTTGGCGCTACCGGCAg                       >  1:1739169/1‑151 (MQ=255)
                                                                                                                 aCATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGTTGGCGCTACCGGCAg                       <  1:3630367/151‑1 (MQ=255)
                                                                                                                 aCATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGTTGGCGCTACCGGCAg                       <  1:1015787/151‑1 (MQ=255)
                                                                                                                  cATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGTTGGCGCTACCGGCAGc                      >  1:2553598/1‑151 (MQ=255)
                                                                                                                   aTCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGTTGGCGCTACCGGCAGCt                     >  2:440778/1‑151 (MQ=255)
                                                                                                                       ccGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGTTGGCGCTACCGGCAGCTTccg                 >  1:3778320/1‑149 (MQ=16)
                                                                                                                        cGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGTTGGCGCTACCGGCAGCTTCAGc                >  1:164308/1‑151 (MQ=255)
                                                                                                                            ccTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGTTGGCGCTACCGGCAGCTTCAGCATcc            >  2:3341938/1‑150 (MQ=255)
                                                                                                                            ccTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGTTGGCGCTACCGGCAGCTTCAGCATCa            <  1:4708542/151‑1 (MQ=255)
                                                                                                                            ccTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGTTGGCGCTACCGGCAGCTTCAGCATCa            >  1:442089/1‑151 (MQ=255)
                                                                                                                            ccTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGTTGGCGCTACCGGCAGCTTCAGCATCa            <  2:5035837/151‑1 (MQ=19)
                                                                                                                             cTGCCGCACCGACAGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGTTGGCGCTACCGGCAGCTTCAGCATCAc           >  1:3162041/1‑151 (MQ=255)
                                                                                                                              tGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGAGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGCAGTTGGCGCTACCGGCAGCTTCAGCATCAcc          >  2:4086841/1‑151 (MQ=255)
                                                                                                                                ccGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGTTGGCGCTACCGGCAGCTTCAGCATCACCCt        >  2:1920608/1‑151 (MQ=255)
                                                                                                                                 cGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGTTGGCGCTACCGGCAGCTTCAGCATCACCCta       >  1:2411438/1‑150 (MQ=255)
                                                                                                                                     ccgaccgaGCGGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGTTGGCGCTACCGGCAGCTTCAGCATCACCCTAGAcc   <  2:3017917/151‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                                   
CGGCAGCTTCACCGTCACCCTCGACCCGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGAGCGAACTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGCACCGTACGCGTGCTGGCCGCCGACGGCACCACTGTGCTGGGTAGCGTGGTAGTTGGCGCTACCGGCAGCTTCAGCATCACCCTCGACCC  >  CP009974/1158262‑1158546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑