BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	1,541,228	T→C	N196S (AAC→AGC)	RPPX_06830 ←	DNA‑directed RNA polymerase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	1,541,228	0	T	C	100.0%	812.9 / NA	226	N196S (AAC→AGC)	RPPX_06830	DNA‑directed RNA polymerase subunit alpha
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (116/110); total (116/110)

Only 100 of 226 total aligned reads displayed.

TCCTGCTCGACTACGACAGGCTCGCTGTCACCTTTGAGGTCGACGAACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGTTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCTTTCGTCTTCGTCGGTTTGACGGGAGTCGGCCGGCTCGTAACCACGACCAC  >  CP009974/1541081‑1541375

 ccTGCTCGACTACGACAGGCTCGCTGTCACCTTTGAGGTCGACGAACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGt                                                                                                                                                 <  1:3917928/151‑1 (MQ=255)

   tGCTCGACTACGACAGGCTCGCTGTCACCTTTGAGGTCGACGAACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTAc                                                                                                                                               >  1:3177427/1‑151 (MQ=255)

   tGCTCGACTACGACAGGCTCGCTGTCACCTTTGAGGTCGACGAACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTAc                                                                                                                                               <  1:2118897/151‑1 (MQ=255)

    gCTCGACTACGACAGGCTCGCTGTCACCTTTGAGGTCGACGAACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACg                                                                                                                                              <  1:2897715/151‑1 (MQ=255)

          cTACGACAGGCTCGCTGTCACCTTTGAGGTCGACGAACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTc                                                                                                                                        <  1:4032243/151‑1 (MQ=255)

                 aGGCTCGCTGTCACCTTTGAGGTCGACGAACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACgg                                                                                                                                 >  1:2497490/1‑151 (MQ=255)

                    cTCGCTGTCACCTTTGAGGTCGACGAACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCg                                                                                                                              >  2:2490459/1‑151 (MQ=255)

                       gCTGTCACCTTTGAGGTCGACGAACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTtc                                                                                                                           <  1:1214004/151‑1 (MQ=255)

                       gCTGTCACCTTTGAGGTCGACGAACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTtc                                                                                                                           >  1:4098353/1‑151 (MQ=255)

                        cTGTCACCTTTGAGGTCGACGAACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTtct                                                                                                                          <  1:444724/151‑1 (MQ=255)

                        cTGTCACCTTTGAGGTCGACGAACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTtct                                                                                                                          <  1:4231783/151‑1 (MQ=255)

                         tGTCACCTTTGAGGTCGACGAACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTtctc                                                                                                                         <  1:68010/151‑1 (MQ=255)

                          gTCACCTTTGAGGTCGACGAACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCg                                                                                                                        >  1:2501920/1‑151 (MQ=255)

                             aCCTTTGAGGTCGACGAACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGAcc                                                                                                                     >  1:2089220/1‑151 (MQ=255)

                              ccTTTGAGGTCGACGAACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACca                                                                                                                    <  2:2301325/151‑1 (MQ=255)

                               cTTTGAGGTCGACGAACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACcac                                                                                                                   >  1:1752283/1‑151 (MQ=255)

                                 ttGAGGTCGACGAACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACAt                                                                                                                 >  2:3361517/1‑151 (MQ=255)

                                 ttGAGGTCGACGAACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACAt                                                                                                                 >  1:3897052/1‑151 (MQ=255)

                                  tggggTCGACGAACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATa                                                                                                                <  2:444770/148‑1 (MQ=255)

                                   ggggTCCACGAACGCAGCCAACTGCGGTTGCAGAATGGTCGAAGCGCGGCGGATAGCCGCTTCAGGATCCAGGGTGGCGTTGGTTGCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAg                                                                                                               <  2:4753103/149‑1 (MQ=255)

                                   gAGGTCGACGAACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGCTCCAGGGTGCCGTTGGTTCCCAGATCAATGACCAGCCTGTCCAGCCCGGTACGCTGCTCCACACGGGCGCTCTCcaccaccccc                                                                                                               >  2:4748528/1‑147 (MQ=255)

                                   gAGGTCGACGAACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAg                                                                                                               <  1:755741/151‑1 (MQ=255)

                                     ggTCGACGAACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGc                                                                                                             >  2:4701560/1‑151 (MQ=255)

                                      gTCGACGAACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCg                                                                                                            >  1:3569467/1‑151 (MQ=255)

                                         gacgaACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATa                                                                                                         >  2:682231/1‑151 (MQ=255)

                                           cgaACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATacg                                                                                                       >  2:2030406/1‑151 (MQ=255)

                                           cgaACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATacg                                                                                                       <  1:4831238/151‑1 (MQ=255)

                                            gaACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTGCAGCCTGGTACGCTGCTCAACACGGGCGTTCTCGACCACATAGGCGATacga                                                                                                      >  2:3277552/1‑151 (MQ=255)

                                             aaCGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATacgac                                                                                                     >  2:584243/1‑151 (MQ=255)

                                                gCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGCGCGTTCTCGACCACACAGCCGATACGACGCa                                                                                                  >  2:2357264/1‑151 (MQ=255)

                                                     cAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCggg                                                                                             <  2:3514701/151‑1 (MQ=255)

                                                      aaCTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGc                                                                                            <  2:857731/151‑1 (MQ=255)

                                                            tgTTGCAGGATGGGCGCGGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAagg                                                                                      <  2:3569467/151‑1 (MQ=255)

                                                            tgTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGCagg                                                                                      >  2:2602346/1‑151 (MQ=255)

                                                            tgTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAagg                                                                                      <  2:628693/151‑1 (MQ=255)

                                                             gTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAagga                                                                                     >  2:3043902/1‑151 (MQ=255)

                                                             gTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAagga                                                                                     >  1:2371644/1‑151 (MQ=255)

                                                             gTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACCCTGTTCCACACGGGCGTTCTCGACCACATAGGCGATACGCCGCACCGGGCCGAagga                                                                                     >  2:4363644/1‑151 (MQ=255)

                                                               tGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAaggagg                                                                                   >  2:5042362/1‑151 (MQ=255)

                                                                gCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGc                                                                                  >  1:3839608/1‑151 (MQ=255)

                                                                gCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGc                                                                                  >  2:1590064/1‑151 (MQ=255)

                                                                  aGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGt                                                                                >  2:2857746/1‑151 (MQ=255)

                                                                   gAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTc                                                                               >  2:2657183/1‑151 (MQ=255)

                                                                   gAATGGTCGAAGCGCGGCGGATAGTCTCTTCAGGATCCAGGGTGCCGTTAGTTTCCAGACCAATGACCAGTATGCCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGTCGATACGACGGACCGGGCTGGGGGAGGCGTc                                                                               <  2:3773238/151‑1 (MQ=255)

                                                                     aTGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCa                                                                             >  1:2022393/1‑151 (MQ=255)

                                                                      tGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCaa                                                                            <  2:246765/151‑1 (MQ=255)

                                                                      tGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCaa                                                                            <  2:1310653/151‑1 (MQ=255)

                                                                      tGGGCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAAGGTGCCGTTGGTTGCCAGATCAATGACCAGCTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCaa                                                                            <  2:4602132/151‑1 (MQ=255)

                                                                        gTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACt                                                                          <  2:1759907/151‑1 (MQ=255)

                                                                          cGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGc                                                                        >  2:4861936/1‑151 (MQ=255)

                                                                            cAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAg                                                                      >  2:4540719/1‑151 (MQ=255)

                                                                             aGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGa                                                                     <  1:1820352/151‑1 (MQ=255)

                                                                             aGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGa                                                                     >  1:594887/1‑151 (MQ=255)

                                                                              gcgcGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGAc                                                                    <  1:5076945/151‑1 (MQ=255)

                                                                                 cggcggATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGc                                                                 <  2:311508/151‑1 (MQ=255)

                                                                                  ggcggATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGcc                                                                >  1:1344388/1‑151 (MQ=255)

                                                                                  ggcggATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGcc                                                                >  1:1313385/1‑151 (MQ=255)

                                                                                    cggATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCaa                                                              >  1:2274922/1‑151 (MQ=255)

                                                                                    cggATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCaa                                                              >  1:540517/1‑151 (MQ=255)

                                                                                     ggATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAAt                                                             >  1:285730/1‑151 (MQ=255)

                                                                                     ggATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAAt                                                             <  1:1997900/151‑1 (MQ=255)

                                                                                      gagaGCCTCTTCAGGATCCAGGGTGCAGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATg                                                            <  2:3879511/148‑1 (MQ=255)

                                                                                      gATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATg                                                            >  1:1083009/1‑151 (MQ=255)

                                                                                       aTAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGc                                                           >  2:2822085/1‑151 (MQ=255)

                                                                                        tAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCt                                                          <  2:2247888/151‑1 (MQ=255)

                                                                                         aGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTa                                                         >  2:1410977/1‑151 (MQ=255)

                                                                                           ccTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACg                                                       <  1:2549051/151‑1 (MQ=255)

                                                                                            ctctTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACgg                                                      >  2:3268870/1‑151 (MQ=255)

                                                                                            ctctTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACgg                                                      <  1:3120823/151‑1 (MQ=255)

                                                                                             tctTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGc                                                     >  2:2236008/1‑151 (MQ=255)

                                                                                              ctTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCt                                                    <  2:1061428/151‑1 (MQ=255)

                                                                                                tCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCttt                                                  <  2:2036975/151‑1 (MQ=255)

                                                                                                tCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCttt                                                  <  1:4643776/151‑1 (MQ=255)

                                                                                                 cAGGATCCAGGGTGCCGTTGGTTTCCAGATTAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGTTACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCTttc                                                 >  1:968850/1‑151 (MQ=255)

                                                                                                 cAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCTttc                                                 >  2:2434600/1‑151 (MQ=255)

                                                                                                 cAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCTttc                                                 <  1:4422319/151‑1 (MQ=255)

                                                                                                 cAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCTttc                                                 >  1:1028192/1‑151 (MQ=255)

                                                                                                   ggATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCTttcgt                                               >  2:934107/1‑151 (MQ=255)

                                                                                                     aTCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCTttcgtct                                             <  2:2231228/151‑1 (MQ=255)

                                                                                                      tCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCTttcgtctt                                            <  2:108736/151‑1 (MQ=255)

                                                                                                      tCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCTttcgtctt                                            <  2:530558/151‑1 (MQ=255)

                                                                                                       ccAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCTttcgtcttc                                           <  1:1865264/151‑1 (MQ=255)

                                                                                                         aGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCTttcgtcttcgt                                         <  2:2104383/151‑1 (MQ=255)

                                                                                                             tGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCTTTCGTCTTCGTCGGt                                     <  1:3712837/151‑1 (MQ=255)

                                                                                                              gCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCTTTCGTCTTCGTCGGtt                                    <  1:4716202/151‑1 (MQ=255)

                                                                                                                   tGGTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCTTTCGTCTTCGTCGGTTTGACg                               <  1:3366547/151‑1 (MQ=255)

                                                                                                                     gTTTCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCTTTCGTCTTCGTCGGTTTGACggg                             <  1:99136/151‑1 (MQ=255)

                                                                                                                       ttCCAGATCAATGACCAGTTTGTCCAGGCTGGTCCGCTGTTCAACACGGGCGTTATCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGACAATGCTACGGCTGTGGTCTTCGGCGGGTTGACGGGAg                           >  1:4263827/1‑151 (MQ=255)

                                                                                                                       ttCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCTTTCGTCTTCGTCGGTTTGACGGGAg                           >  1:4594253/1‑151 (MQ=255)

                                                                                                                       ttCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCTTTCGTCTTCGTCGGTTTGACGGGAg                           >  1:1173136/1‑151 (MQ=255)

                                                                                                                       ttCCAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCTTTCGTCTTCGTCGGTTTGACGGGAg                           >  2:1222860/1‑151 (MQ=255)

                                                                                                                         ccAGATCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGTCGGCCAATGCTACGGCTTTCGTCTTCGTCGGTTTGACGGGAGTc                         <  1:3936405/151‑1 (MQ=255)

                                                                                                                              tCAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCTTTCGTCTTCGTCGGTTTGACGGGAGTcggccg                    <  2:4002476/151‑1 (MQ=255)

                                                                                                                               cAATGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCTTTCGTCTTCGTCGGTTTGACGGGAGTcggccgg                   >  1:1164957/1‑151 (MQ=255)

                                                                                                                                aaTGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCTTTCGTCTTCGTCGGTTTGACGGGAGTcggccggc                  >  2:1438391/1‑151 (MQ=255)

                                                                                                                                  tGACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCTTTCGTCTTCGTCGGTTTGACGGGAGTCGGCCGGCTc                <  2:3101716/151‑1 (MQ=255)

                                                                                                                                   gACCAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCTTTCGTCTTCGTCGGTTTGACGGGAGTCGGCCGGCTCg               >  1:1133407/1‑151 (MQ=255)

                                                                                                                                      cAGTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCTTTCGTCTTCGTCGGTTTGACGGGAGTCGGCCGGCTCGTaa            >  1:5139590/1‑151 (MQ=255)

                                                                                                                                        gTTTGTCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCTTTCGTCTTCGTCGGTTTGACGGGAGTCGGCCGGCTCGTAacc          >  1:4319604/1‑151 (MQ=255)

                                                                                                                                             tCCAGGCTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCTTTCGTCTTCGTCGGTTTGACGGGAGTCGGCCGGCTCGTAaccacgac     <  1:1008387/151‑1 (MQ=255)

TCCTGCTCGACTACGACAGGCTCGCTGTCACCTTTGAGGTCGACGAACGCAGCCAACTGCTGTTGCAGAATGGTCGCAGCGCGGCGGATAGCCTCTTCAGGATCCAGGGTGCCGTTGGTTTCCAGATCAATGACCAGTTTGTCCAGGTTGGTACGCTGTTCAACACGGGCGTTCTCGACCACATAGGCGATACGACGCACCGGGCTGAAGGAGGCGTCCAACTGCAGACGGCCAATGCTACGGCTTTCGTCTTCGTCGGTTTGACGGGAGTCGGCCGGCTCGTAACCACGACCAC  >  CP009974/1541081‑1541375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`