BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	1,554,345	A→T	V354V (GTT→GTA)	tuf ←	elongation factor Tu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	1,554,345	0	A	T	99.4%	428.5 / NA	163	V354V (GTT→GTA)	tuf	elongation factor Tu
Reads supporting (aligned to +/- strand): ref base A (0/1); new base T (74/88); total (74/89)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 163 total aligned reads displayed.

TCAATCGGTCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATAACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTGGTG  >  CP009974/1554197‑1554492

tCAATCGGTCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGTATTAc                                                                                                                                                   >  1:1533818/1‑151 (MQ=255)

    tCGGTCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCAtt                                                                                                                                               <  1:2206035/151‑1 (MQ=255)

     cGGTCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCAttt                                                                                                                                              <  2:1775006/151‑1 (MQ=255)

      ggTCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTc                                                                                                                                             <  2:3871846/151‑1 (MQ=255)

       gTCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCa                                                                                                                                            >  1:2829685/1‑151 (MQ=255)

        tCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCaa                                                                                                                                           >  1:1370414/1‑151 (MQ=255)

         cGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAAc                                                                                                                                          <  2:311903/151‑1 (MQ=255)

         cGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAAc                                                                                                                                          <  1:5119166/151‑1 (MQ=255)

          gAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACg                                                                                                                                         <  1:4459625/151‑1 (MQ=255)

          gAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACg                                                                                                                                         >  2:4908168/1‑151 (MQ=255)

           aGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGc                                                                                                                                        >  1:607760/1‑151 (MQ=255)

               aTTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTc                                                                                                                                    >  1:1052259/1‑151 (MQ=255)

               aTTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTc                                                                                                                                    <  2:1372249/151‑1 (MQ=255)

                ttACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACgtgttac                                                                                                                                   >  2:3610609/1‑145 (MQ=255)

                ttACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTcc                                                                                                                                   <  2:3890576/151‑1 (MQ=255)

                ttACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTcc                                                                                                                                   <  2:3438952/151‑1 (MQ=255)

                ttACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTcc                                                                                                                                   <  1:1510744/151‑1 (MQ=255)

                ttACTCGATGATTTTTGCCACAACGCCGGCGCCGACGATACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTcc                                                                                                                                   <  2:699363/151‑1 (MQ=255)

                 ttctctATGATTGTTGCAACAACGCCGCCGCCGACGGTACGACCGCCATCACGGATAGCGAAGCCCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCAGTTCAACGCCTTCCg                                                                                                                                  <  2:4339229/149‑1 (MQ=11)

                 tACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCg                                                                                                                                  <  1:3758828/151‑1 (MQ=255)

                 tACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCg                                                                                                                                  >  1:4244181/1‑151 (MQ=255)

                   cTCGATGATTTTTGCCCCACCGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGc                                                                                                                                <  2:1596636/151‑1 (MQ=255)

                   cTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGc                                                                                                                                >  2:736613/1‑151 (MQ=255)

                    tCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTAACACTTCAACGCCCTCCGGcc                                                                                                                               >  1:1541377/1‑150 (MQ=255)

                     cGATTATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAg                                                                                                                              <  2:1931633/151‑1 (MQ=25)

                     cGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAg                                                                                                                              <  2:927907/151‑1 (MQ=38)

                       atgatTTTTGCCACAAGGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGtt                                                                                                                            <  2:4342264/151‑1 (MQ=255)

                        tgatTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTc                                                                                                                           <  2:241224/151‑1 (MQ=25)

                        taatTTTTGCCACAACGCCGGCGACGACGGTCCGGCCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGGCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTc                                                                                                                           <  1:3061794/149‑1 (MQ=11)

                         gatTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCg                                                                                                                          <  2:4902642/151‑1 (MQ=25)

                            ttttGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAg                                                                                                                       >  2:3050059/1‑151 (MQ=18)

                             tttGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGt                                                                                                                      >  1:3282743/1‑151 (MQ=25)

                             tttGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGt                                                                                                                      >  1:4607978/1‑151 (MQ=25)

                              ttGCCACAACGCCGGCGCCGACGGTACGCCCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCTGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGtt                                                                                                                     <  2:3915967/151‑1 (MQ=16)

                              ttGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGtt                                                                                                                     >  1:4678198/1‑151 (MQ=21)

                              ttGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGtt                                                                                                                     <  1:4975176/151‑1 (MQ=21)

                              ttGCCACAACGCCGGCGACGACGGTACGACCGACTTCACGGATAGCGAAGCGCAGACAGTCTTCCATTGCGATGGTCTTGATCAGAGTAACAGTCATCTGAATGTTGTCACCTTTCATAATCATTTCAACGCCTTCCGGCAGTTCGCAGtt                                                                                                                     <  2:3430411/151‑1 (MQ=11)

                                gCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTAc                                                                                                                   <  2:3471951/151‑1 (MQ=18)

                                gCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTAc                                                                                                                   <  1:4983404/151‑1 (MQ=18)

                                 ccACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTAcc                                                                                                                  <  2:3758836/151‑1 (MQ=18)

                                 ccACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTAcc                                                                                                                  >  2:239654/1‑151 (MQ=18)

                                 ccACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTAcc                                                                                                                  >  2:3104327/1‑151 (MQ=18)

                                  cacaACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCg                                                                                                                 <  2:2599040/151‑1 (MQ=17)

                                   acaACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAAAGCCTTCCGGCAGTTCGCAGTTACCgg                                                                                                                >  2:939961/1‑151 (MQ=14)

                                     aaCGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTc                                                                                                              <  1:3007960/151‑1 (MQ=11)

                                     aaCGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTc                                                                                                              >  1:3033696/1‑151 (MQ=11)

                                                                                                           ttGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCcttctt                                        <  2:318276/151‑1 (MQ=14)

                                                                                                           ttGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCcttctt                                        <  1:4403329/151‑1 (MQ=14)

                                                                                                             gATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTcttcc                                      >  1:679112/1‑151 (MQ=17)

                                                                                                             gATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTcttcc                                      >  1:4009643/1‑151 (MQ=17)

                                                                                                             gATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTcttcc                                      >  1:3628494/1‑151 (MQ=14)

                                                                                                             gATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTcttcc                                      <  2:812653/151‑1 (MQ=14)

                                                                                                             gATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTcttcc                                      >  2:4125611/1‑151 (MQ=14)

                                                                                                              aTCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTcttcct                                     >  2:1905691/1‑151 (MQ=17)

                                                                                                              aTCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTcttcct                                     <  1:2060390/151‑1 (MQ=14)

                                                                                                              aTCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTcttcct                                     >  2:1307068/1‑151 (MQ=14)

                                                                                                               tCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTcttcctt                                    <  1:3324494/151‑1 (MQ=17)

                                                                                                                cAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTcttccttc                                   <  1:1207045/151‑1 (MQ=17)

                                                                                                                cAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTcttccttc                                   >  2:3504697/1‑151 (MQ=14)

                                                                                                                cAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTGACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTTGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTcttccttc                                   <  1:3449666/151‑1 (MQ=9)

                                                                                                                 aGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCg                                  >  1:4128781/1‑151 (MQ=17)

                                                                                                                 aGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCg                                  >  2:3046498/1‑151 (MQ=14)

                                                                                                                 aGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCg                                  <  2:2085625/151‑1 (MQ=17)

                                                                                                                 aGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCg                                  >  1:2922393/1‑151 (MQ=17)

                                                                                                                  gggTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGa                                 >  2:1685231/1‑151 (MQ=14)

                                                                                                                  gggTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGa                                 >  1:2205049/1‑151 (MQ=17)

                                                                                                                    gTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACa                               <  1:274726/151‑1 (MQ=14)

                                                                                                                    gTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACa                               >  2:1228472/1‑151 (MQ=17)

                                                                                                                     tagcggTCATCTGAATGTTGTCGCCTGGCATTACCATTTCAGCGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAGCTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAg                              <  2:2228514/146‑1 (MQ=11)

                                                                                                                     tAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAg                              >  1:2144291/1‑151 (MQ=17)

                                                                                                                     tAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAg                              >  1:606169/1‑151 (MQ=17)

                                                                                                                       aCAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGa                            >  1:3383592/1‑151 (MQ=17)

                                                                                                                        cAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGAc                           <  2:667680/151‑1 (MQ=14)

                                                                                                                         aGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACg                          <  2:5200569/151‑1 (MQ=17)

                                                                                                                          gTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGt                         <  2:1000920/151‑1 (MQ=14)

                                                                                                                           tCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTa                        >  2:4641027/1‑151 (MQ=17)

                                                                                                                           tCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTa                        <  1:970479/151‑1 (MQ=17)

                                                                                                                            cATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAg                       <  2:3033696/151‑1 (MQ=17)

                                                                                                                            cATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAg                       <  2:2895464/151‑1 (MQ=17)

                                                                                                                             aTCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTATCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGa                      >  2:3286294/1‑151 (MQ=17)

                                                                                                                              tCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGAc                     >  1:355629/1‑151 (MQ=17)

                                                                                                                               cTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACt                    <  1:1657700/151‑1 (MQ=17)

                                                                                                                                tGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACtt                   >  1:2630959/1‑151 (MQ=17)

                                                                                                                                tGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACtt                   >  1:4374161/1‑151 (MQ=14)

                                                                                                                                 gAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTc                  >  1:4356561/1‑151 (MQ=17)

                                                                                                                                  aaTGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCt                 <  2:1479696/151‑1 (MQ=17)

                                                                                                                                  aaTGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCt                 <  1:4315574/151‑1 (MQ=17)

                                                                                                                                    tgttgtCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGTCGTAGACTTCTGc               >  2:89490/1‑151 (MQ=17)

                                                                                                                                    tgttgtCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGc               >  1:3780479/1‑151 (MQ=17)

                                                                                                                                      ttgtCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCgg             >  1:3104093/1‑151 (MQ=14)

                                                                                                                                       tgtCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGt            <  1:537865/151‑1 (MQ=14)

                                                                                                                                       tgtCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGt            <  1:537879/151‑1 (MQ=17)

                                                                                                                                       tgtCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGt            >  2:5081121/1‑151 (MQ=14)

                                                                                                                                       tgtCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGt            <  2:2843959/151‑1 (MQ=17)

                                                                                                                                        gtCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTg           >  1:2581872/1‑151 (MQ=17)

                                                                                                                                        gtCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTg           >  1:2299652/1‑151 (MQ=14)

                                                                                                                                         tCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGa          >  1:111142/1‑151 (MQ=17)

                                                                                                                                          cACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGaa         >  2:1964379/1‑151 (MQ=17)

                                                                                                                                              tGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTg     <  2:171055/151‑1 (MQ=17)

                                                                                                                                               ggCATTACCATTTCAACGCCTTCCGGTAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTgg    <  1:910787/151‑1 (MQ=14)

TCAATCGGTCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATAACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTGGTG  >  CP009974/1554197‑1554492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`