BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	1,554,630	A→G	C259C (TGT→TGC)	tuf ←	elongation factor Tu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	1,554,630	0	A	G	99.5%	464.1 / ‑5.0	206	C259C (TGT→TGC)	tuf	elongation factor Tu
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (90/115); total (90/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

Only 100 of 206 total aligned reads displayed.

CTGGCCACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTACAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGCCCCGAGATCGAGAATACGTCTTCGATTGGCATCAGGAACGGCTGGTC  >  CP009974/1554525‑1554776

 tGGCCACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGAt                                                                                                      <  2:3780479/151‑1 (MQ=14)

   gCCACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATcc                                                                                                    >  1:2224889/1‑151 (MQ=14)

    ccACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCt                                                                                                   <  1:1050779/151‑1 (MQ=14)

    ccACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCt                                                                                                   >  1:4395686/1‑151 (MQ=14)

    ccACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCt                                                                                                   >  1:675876/1‑151 (MQ=14)

    ccACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCt                                                                                                   >  2:1007084/1‑151 (MQ=14)

     cACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTg                                                                                                  <  2:1637925/151‑1 (MQ=17)

      aCGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGa                                                                                                 <  1:3104327/151‑1 (MQ=17)

       cGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGaa                                                                                                <  2:2081878/151‑1 (MQ=17)

        gCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAAc                                                                                               >  2:1689191/1‑151 (MQ=17)

          tCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAAcgc                                                                                             >  1:2849044/1‑151 (MQ=14)

          tCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGACGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAAcgc                                                                                             <  2:3270857/151‑1 (MQ=17)

           cAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAAcgcg                                                                                            <  1:1315935/151‑1 (MQ=17)

            aaCGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCgg                                                                                           >  1:1618752/1‑151 (MQ=17)

            aaCGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCgg                                                                                           <  2:2955130/151‑1 (MQ=17)

              cgtcgtCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGAc                                                                                         <  2:2922393/151‑1 (MQ=14)

                tcgtcACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGa                                                                                       >  1:1093987/1‑151 (MQ=14)

                   tcACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATAc                                                                                    >  1:3662335/1‑151 (MQ=17)

                   tcACGCCTGGTCCCACGCAGCGGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATAc                                                                                    <  2:1541377/151‑1 (MQ=9)

                   gcACGCTTGTTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGGCCAGCAGCTTGCGGAACCTCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATAc                                                                                    <  2:4103587/150‑1 (MQ=255)

                     ccGCTTGGTACCACGCAGCAGACCGCCGCAGTTCTCGCCAGCACGAGCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCa                                                                                  <  2:3581617/150‑1 (MQ=9)

                      cGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCAc                                                                                 <  2:1930158/151‑1 (MQ=17)

                        cTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGc                                                                               <  1:3225506/151‑1 (MQ=17)

                         ttGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCt                                                                              >  1:476781/1‑151 (MQ=17)

                           ggTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCg                                                                            >  1:4066909/1‑151 (MQ=14)

                           ggTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCg                                                                            <  2:1236326/151‑1 (MQ=17)

                              aCCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATa                                                                         <  2:3383592/151‑1 (MQ=17)

                              aCCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATa                                                                         <  1:2626140/151‑1 (MQ=17)

                               ccACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATAc                                                                        <  2:3639157/151‑1 (MQ=17)

                               ccACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATAc                                                                        <  1:372130/151‑1 (MQ=17)

                               ccACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTACAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATAc                                                                        <  1:3783307/151‑1 (MQ=32)

                                cACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACg                                                                       <  1:1972336/151‑1 (MQ=17)

                                   gcagcaGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGAcc                                                                    >  1:800650/1‑151 (MQ=17)

                                    cagcagAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCg                                                                   <  1:4504815/151‑1 (MQ=17)

                                     agcagAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCgg                                                                  >  1:2358480/1‑151 (MQ=14)

                                     agcagAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCgg                                                                  >  2:3979494/1‑151 (MQ=17)

                                         gAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTaac                                                              >  1:3685090/1‑151 (MQ=17)

                                           aCGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGACCATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTaacaa                                                            <  2:4577121/151‑1 (MQ=17)

                                            cgccgcAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTaacaac                                                           >  1:587935/1‑151 (MQ=17)

                                             gccgcAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACg                                                          <  2:617541/151‑1 (MQ=17)

                                             gccgcAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACg                                                          <  1:2987865/151‑1 (MQ=17)

                                             gccgcAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACg                                                          >  1:716595/1‑151 (MQ=17)

                                              ccgcAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACgg                                                         <  2:2523770/151‑1 (MQ=17)

                                               cgcAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGt                                                        >  2:1509411/1‑151 (MQ=17)

                                               cgcAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGt                                                        <  2:3292216/151‑1 (MQ=17)

                                               cgcAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGt                                                        <  1:1509411/151‑1 (MQ=17)

                                               cgcAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGCGGTGGTGGTGGGGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGt                                                        >  2:1960836/1‑151 (MQ=9)

                                                gcAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTa                                                       <  2:4374161/151‑1 (MQ=17)

                                                ccAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTa                                                       <  2:5076227/150‑1 (MQ=17)

                                                 cAGTTCTCGCCAGCACGACCTTCGTCCAGCATGTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTAc                                                      <  1:1647591/151‑1 (MQ=12)

                                                  aGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTAcc                                                     >  2:3729514/1‑151 (MQ=17)

                                                   gTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGCTTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTACCAACGGTAccc                                                    >  1:4681611/1‑150 (MQ=9)

                                                    ttCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCAc                                                   <  2:2147268/151‑1 (MQ=17)

                                                    ttCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCAc                                                   <  2:1370414/151‑1 (MQ=14)

                                                     tctcGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACg                                                  <  1:1683288/151‑1 (MQ=17)

                                                        cGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGacc                                               >  1:537130/1‑151 (MQ=14)

                                                         gCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGaccc                                              <  2:4761479/151‑1 (MQ=14)

                                                         gCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGaccc                                              <  2:4356561/151‑1 (MQ=17)

                                                         gCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGGGCCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACGACGaccc                                              >  1:1345758/1‑151 (MQ=11)

                                                          ccAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCg                                             <  2:1132089/151‑1 (MQ=14)

                                                           cAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCga                                            <  1:2409386/151‑1 (MQ=12)

                                                           cAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCga                                            >  1:4260077/1‑151 (MQ=17)

                                                            aGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCgag                                           <  1:2922157/151‑1 (MQ=12)

                                                             gCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCgaga                                          <  1:3009944/151‑1 (MQ=17)

                                                              cACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGAt                                         >  1:1984871/1‑151 (MQ=14)

                                                              cACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGAt                                         <  1:2507801/151‑1 (MQ=17)

                                                               aCGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATc                                        <  2:4922390/151‑1 (MQ=9)

                                                                cGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCg                                       >  2:2581737/1‑151 (MQ=14)

                                                                 gACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCga                                      <  2:2048465/151‑1 (MQ=12)

                                                                    cTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGaa                                   <  2:2599766/151‑1 (MQ=14)

                                                                     ttCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGaac                                  >  1:4421287/1‑150 (MQ=12)

                                                                     ttCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGaac                                  >  1:1466064/1‑150 (MQ=12)

                                                                      tCGTCCAGCAGCTTGCGGAACATCTCAGCACCGGTGCTGGTGGTGGTGGTGGTGTCGCGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGTCGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGaaca                                 <  2:2255854/151‑3 (MQ=11)

                                                                       cGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAacac                                <  1:1947927/151‑1 (MQ=12)

                                                                           cagcagCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCt                            <  2:460394/151‑1 (MQ=12)

                                                                            agcagcTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCtt                           >  2:464615/1‑151 (MQ=12)

                                                                             gcagcTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGACCGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTc                          <  1:637982/151‑1 (MQ=255)

                                                                              cagcTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCg                         >  1:424717/1‑151 (MQ=255)

                                                                                 cTTGCGGAACATCTCAACGCCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGAtt                      <  2:789616/151‑1 (MQ=255)

                                                                                  ttGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTg                     >  2:2857903/1‑151 (MQ=12)

                                                                                  ttGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTg                     >  2:3943249/1‑151 (MQ=12)

                                                                                     gggAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTGACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCa                  <  2:4009643/150‑1 (MQ=255)

                                                                                     cGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAAAACGTCTTCGATTGGCa                  >  2:4878479/1‑151 (MQ=12)

                                                                                      ggAACATCTCAACACCGGTGGAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCAt                 <  2:4740159/151‑1 (MQ=255)

                                                                                      ggAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCAt                 >  1:1881621/1‑151 (MQ=12)

                                                                                      ggAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCAt                 <  2:2205049/151‑1 (MQ=255)

                                                                                      ggAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCAt                 >  2:1293415/1‑151 (MQ=12)

                                                                                           aTCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGa            >  2:2283611/1‑151 (MQ=255)

                                                                                            tctcAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGaa           >  1:174943/1‑151 (MQ=255)

                                                                                            tctcAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGaa           <  1:4967485/151‑1 (MQ=255)

                                                                                             ctcGACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCACCGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAAc          <  2:605138/151‑1 (MQ=255)

                                                                                             ctcAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAAc          >  2:844740/1‑151 (MQ=9)

                                                                                             ctcAACACCGGTGCAGGTGGTGGTGGTCGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGCGATCGAGAACACGTCTTCGATTGGCATCAGGAAc          <  2:5169030/151‑1 (MQ=11)

                                                                                              tcAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACg         <  2:3173396/151‑1 (MQ=12)

                                                                                                aaCACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGc       >  1:255130/1‑151 (MQ=255)

                                                                                                 acacCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCt      >  1:3333707/1‑151 (MQ=12)

                                                                                                 acacCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCACCAGGAACGGCt      <  1:3421159/151‑1 (MQ=11)

                                                                                                  cacCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTg     >  1:1276563/1‑151 (MQ=12)

                                                                                                     cGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTc  <  1:3943249/151‑1 (MQ=12)

                                                                                                     cGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTc  <  1:1819236/151‑1 (MQ=255)

CTGGCCACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTACAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGCCCCGAGATCGAGAATACGTCTTCGATTGGCATCAGGAACGGCTGGTC  >  CP009974/1554525‑1554776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`