BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	1,554,744	T→C	V221V (GTA→GTG)	tuf ←	elongation factor Tu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	1,554,744	0	T	C	99.6%	773.0 / NA	248	V221V (GTA→GTG)	tuf	elongation factor Tu
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (112/135); minor base A (1/0); total (113/135)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 250 total aligned reads displayed.

CGTCCAGCAGCTTGCGGAACATCTCAACACCGGTACAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGCCCCGAGATCGAGAATACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGAACCG  >  CP009974/1554596‑1554891

cGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAacac                                                                                                                                                   <  1:1964379/151‑1 (MQ=12)

  tCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGt                                                                                                                                                 >  2:2233281/1‑151 (MQ=9)

   ccAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTc                                                                                                                                                >  1:2391306/1‑151 (MQ=255)

    cagcagCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCt                                                                                                                                               <  2:460394/151‑1 (MQ=12)

          cTTGCGGAACATCTCAACGCCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGAtt                                                                                                                                         <  2:789616/151‑1 (MQ=255)

            tGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGATGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTgg                                                                                                                                       <  2:1533818/151‑1 (MQ=9)

              cGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGCTCGAGAACACGTCTTCGATTGGcc                                                                                                                                     >  2:4878514/1‑150 (MQ=255)

               ggAACATCTCAACACCGGTGGAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCAt                                                                                                                                    <  2:4740159/151‑1 (MQ=255)

               ggAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCAt                                                                                                                                    >  2:1293415/1‑151 (MQ=12)

                    aTCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGa                                                                                                                               <  2:1457728/151‑1 (MQ=12)

                     tctcAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGaa                                                                                                                              <  1:3046498/151‑1 (MQ=255)

                     tctcAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGaa                                                                                                                              <  1:4967485/151‑1 (MQ=255)

                      ctcGACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCACCGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAAc                                                                                                                             <  2:605138/151‑1 (MQ=255)

                       tcAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACg                                                                                                                            <  2:1572869/151‑1 (MQ=255)

                         aaCACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGc                                                                                                                          >  2:4662114/1‑151 (MQ=255)

                         aaCACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGc                                                                                                                          >  1:255130/1‑151 (MQ=255)

                          acacCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCt                                                                                                                         >  1:3333707/1‑151 (MQ=12)

                          acacCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCACCAGGAACGGCt                                                                                                                         <  1:3421159/151‑1 (MQ=11)

                              cGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTc                                                                                                                     <  1:3943249/151‑1 (MQ=12)

                               ggtgcAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCg                                                                                                                    <  1:1177547/147‑1 (MQ=255)

                                   catgtggtagtggtggtgTCACGCAGACCAACTATTTCCAGCGGATCCAGAACGCGGACGATACCACGCTCGATACGACCGATAACAACGTTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATgg                                                                                                                <  2:869810/148‑1 (MQ=11)

                                   cAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATgg                                                                                                                <  1:1031797/151‑1 (MQ=12)

                                    aGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGc                                                                                                               >  1:1902351/1‑151 (MQ=17)

                                      gtggtggtggtggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCGGGTCGATGGCAc                                                                                                             >  2:2456604/1‑151 (MQ=12)

                                         gtggtggtggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGaa                                                                                                          >  2:1718137/1‑151 (MQ=12)

                                          gggtggtggtgTCACGCAGACCAACGATTTCCATCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAAc                                                                                                         <  2:1831279/150‑1 (MQ=9)

                                           ggtggtggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACt                                                                                                        >  2:1477838/1‑151 (MQ=12)

                                           ggtggtggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACt                                                                                                        <  1:2242045/151‑1 (MQ=17)

                                            gtggtggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTg                                                                                                       <  2:2099758/151‑1 (MQ=17)

                                            gtggtggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTg                                                                                                       >  2:322560/1‑151 (MQ=17)

                                               gtggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCt                                                                                                    <  1:729978/151‑1 (MQ=12)

                                                   tgtCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAgg                                                                                                <  2:2507430/151‑1 (MQ=12)

                                                     tCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGa                                                                                              >  2:920879/1‑151 (MQ=16)

                                                       aCGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATg                                                                                            >  1:2351721/1‑151 (MQ=255)

                                                           aGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAgg                                                                                        <  2:2997807/151‑1 (MQ=25)

                                                              ccAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCAt                                                                                     >  1:3350026/1‑151 (MQ=255)

                                                               cAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATc                                                                                    <  2:1940181/151‑1 (MQ=255)

                                                                 aCGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCa                                                                                  <  2:660202/151‑1 (MQ=255)

                                                                 aCGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCa                                                                                  >  1:5010473/1‑151 (MQ=255)

                                                                     tttCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGt                                                                              >  1:4649532/1‑151 (MQ=255)

                                                                      ttCCAGCGGATGCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGtt                                                                             <  2:2699548/151‑1 (MQ=255)

                                                                       tCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGttt                                                                            >  1:2488043/1‑151 (MQ=255)

                                                                       tCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGttt                                                                            <  2:1984871/151‑1 (MQ=255)

                                                                        ccAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTc                                                                           <  2:45461/151‑1 (MQ=255)

                                                                         cggCGGATCCTGAACGCGGACGAGACCACGCTCGATACGAGCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCt                                                                          <  2:3554596/149‑1 (MQ=255)

                                                                          aGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTa                                                                         >  2:1590654/1‑151 (MQ=255)

                                                                          aGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTa                                                                         <  1:1793724/151‑1 (MQ=255)

                                                                           gCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTAc                                                                        >  2:3417280/1‑151 (MQ=255)

                                                                            cGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTAcc                                                                       <  2:262555/151‑1 (MQ=255)

                                                                            cGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTAcc                                                                       <  2:4421287/151‑1 (MQ=255)

                                                                            cGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTAcc                                                                       <  1:2049623/151‑1 (MQ=255)

                                                                             ggATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCa                                                                      >  1:2694261/1‑151 (MQ=255)

                                                                                tCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGCTGTAGGCATCCAGAGTTTCTACCAGct                                                                   >  1:3713478/1‑151 (MQ=255)

                                                                                   tGAACGCGGACGATACCACGCTCGATACGCCCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGcttct                                                                <  2:2727790/151‑1 (MQ=255)

                                                                                   tGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGcttct                                                                >  1:3670145/1‑151 (MQ=255)

                                                                                    gAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGcttctt                                                               <  2:4128781/151‑1 (MQ=255)

                                                                                    gAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGcttctt                                                               <  2:3749593/151‑1 (MQ=255)

                                                                                    gAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGGACCCCGACCCGAGAGCGAGGCCACCGCTTCGGTTGGGATCAGGGACGGCTGGTCGGTGGCGCGGCCTGGCTCAGGGTTGTAGGGATCCAGGGTTTTTCCCAGgtcctt                                                               >  2:1129608/1‑151 (MQ=2)

                                                                                     aaCGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTg                                                              <  2:2888626/151‑1 (MQ=255)

                                                                                      aCGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGa                                                             <  1:3998066/151‑1 (MQ=255)

                                                                                      aCGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGa                                                             >  1:979714/1‑151 (MQ=255)

                                                                                        gcgGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACa                                                           <  1:1580536/151‑1 (MQ=255)

                                                                                        gcgGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACa                                                           <  2:2144291/151‑1 (MQ=255)

                                                                                        gcgGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACa                                                           >  1:4768269/1‑151 (MQ=255)

                                                                                        gcgGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACa                                                           <  1:1917754/151‑1 (MQ=255)

                                                                                            aCGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCgg                                                       >  1:313230/1‑151 (MQ=255)

                                                                                              gATACCACGCTCGCTACGACCGGTAACAACGGCACCACGACCCGAGATCGTGAACACGTCTTCGATTGGAATCAGGAACGGCTGGTCGATGGCACGAACAGGCTAAGTGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGAGGTa                                                     >  2:400278/1‑151 (MQ=14)

                                                                                               aTACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAg                                                    <  2:4219854/151‑1 (MQ=255)

                                                                                                  ccACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGc                                                 >  1:4108074/1‑151 (MQ=255)

                                                                                                    aCGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGccc                                               <  2:3972170/151‑1 (MQ=255)

                                                                                                    aCGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGccc                                               >  2:3636517/1‑151 (MQ=255)

                                                                                                     cGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCa                                              <  2:4395686/151‑1 (MQ=255)

                                                                                                       cTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCAtt                                            >  1:4286428/1‑151 (MQ=255)

                                                                                                        tCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCAttt                                           >  1:4168738/1‑151 (MQ=255)

                                                                                                        tCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCAttt                                           >  1:3010376/1‑151 (MQ=255)

                                                                                                          gATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCg                                         >  1:5212552/1‑151 (MQ=255)

                                                                                                           gtACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGt                                        <  2:3736456/150‑1 (MQ=255)

                                                                                                              cGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTgt                                     <  1:4487448/151‑1 (MQ=255)

                                                                                                                aCCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTgtag                                   >  2:1048918/1‑149 (MQ=255)

                                                                                                                  cGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTgtcgtc                                 >  1:1002580/1‑151 (MQ=255)

                                                                                                                  cGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTgtcgtc                                 >  2:356810/1‑151 (MQ=255)

                                                                                                                   ggTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCt                                <  1:920849/151‑1 (MQ=255)

                                                                                                                     tAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCttt                              >  1:2653338/1‑151 (MQ=255)

                                                                                                                      aacaacGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTg                             >  2:3878203/1‑151 (MQ=255)

                                                                                                                            ggTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTcc                       >  2:2546441/1‑151 (MQ=255)

                                                                                                                              tACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAg                     <  1:1282325/151‑1 (MQ=255)

                                                                                                                              tACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAg                     <  2:3831299/151‑1 (MQ=255)

                                                                                                                                  aCGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGcc                 <  2:3123003/151‑1 (MQ=255)

                                                                                                                                   cgacccGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCa                >  2:2217704/4‑151 (MQ=255)

                                                                                                                                    gacccGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCAt               <  2:5147518/149‑1 (MQ=255)

                                                                                                                                     acccGAGATCGAGAACACGTCTTCGATTGGCATGGGGAACGGCTGGTCGCTGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTGGCCTTCCAGGGCCATa              <  2:5026268/150‑1 (MQ=14)

                                                                                                                                     acccGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATa              >  1:3207391/2‑151 (MQ=255)

                                                                                                                                         gagaTCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAg          >  1:1683779/1‑151 (MQ=255)

                                                                                                                                            aTCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCgg       >  2:4848798/1‑151 (MQ=255)

                                                                                                                                              cGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCCCAGGGATGTAGGCACCCAGAGTTTCTACCAGCTACTTGACAGCGGTAGTGCCCATCTCGTTGTCGTCTTTGCCTTCAAGGGCCATACGAGCGTaa     >  1:2270529/1‑151 (MQ=14)

                                                                                                                                               gagaACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGAAc    <  1:1527790/151‑1 (MQ=255)

                                                                                                                                               gagaACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGAAc    <  2:1515951/151‑1 (MQ=255)

                                                                                                                                                agaACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCCGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGAAcc   <  1:358539/151‑1 (MQ=255)

                                                                                                                                                agaACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGAAcc   >  1:575136/1‑151 (MQ=255)

                                                                                                                                                 gaACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGAACCg  >  2:909395/1‑151 (MQ=255)

CGTCCAGCAGCTTGCGGAACATCTCAACACCGGTACAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGCCCCGAGATCGAGAATACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGAACCG  >  CP009974/1554596‑1554891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`