Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 1,586,415 G→A G53S (GGC→AGC)  RPPX_07115 → membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099741,586,4150GA86.1% 759.0 / 83.5 274G53S (GGC→AGC) RPPX_07115membrane protein
Reads supporting (aligned to +/- strand):  ref base G (16/22);  new base A (113/122);  total (130/144)
Fisher's exact test for biased strand distribution p-value = 6.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.78e-01

Only 100 of 280 total aligned reads displayed.

CCTATGGATCAAAGCGCTGCATATCGTCAGCGTGGTCTGCTGGTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCGGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTGGTCATCCTGCTGACCGGCTACCATCACGTATGCGGTGCCCA  >  CP009974/1586267‑1586563
                                                                                                                                                    |                                                                                                                                                    
 cTATGGATCAAAGCGCTGCATATCGTCAGCGTGGTCTGCTGGTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCgcagca                                                                                                                                                   <  2:3321625/151‑1 (MQ=255)
  tATGGATCAAAGCGCTGCATATCGTCAGCGTGGTCTGCTGGTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGcat                                                                                                                                                  <  2:3092276/151‑1 (MQ=255)
   aTGGATCAAAGCGCTGCATATCGTCAGCGTGGTCTGCTGGTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGcatc                                                                                                                                                 <  1:5153937/151‑1 (MQ=255)
    tGGATCAAAGCGCTGCATATCGTCAGCGTGGTCTGCTGGTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGcatca                                                                                                                                                >  1:1053008/1‑151 (MQ=255)
    tGGATCAAAGCGCTGCATATCGTCAGCGTGGTCTGCTGGTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGcatca                                                                                                                                                >  1:1965812/1‑151 (MQ=255)
        tCAAAGCGCTGCATATCGTCAGCGTGGTCTGCTGGTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCGGCATCATGaa                                                                                                                                            >  2:1260600/1‑151 (MQ=255)
        tCAAAGCGCTGCATATCGTCAGCGTGGTCTGCTGGTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGaa                                                                                                                                            <  2:2469870/151‑1 (MQ=255)
          aaaGCGCTGCATATCGTCAGCGTGGTCTGCTGGTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAAcc                                                                                                                                          <  1:465453/151‑1 (MQ=255)
           aaGCGCTGCATATCGTCAGCGTGGTCTGCTGGTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAAccc                                                                                                                                         <  1:2470930/151‑1 (MQ=255)
               gcTGGAAATCTTGAGCGTTGTTTGCTGGTTCGCATGCCTGTCCTGCATGCCCCGGCTGTTCGTCTCCCCTGCCCAGAGCCAGGACAGCATCCGCCAGGAACGCTTAGTTACGATGGGGCGCATGCTGTACCGCGGCATCTTGATCCCGGCg                                                                                                                                     <  2:4782553/151‑1 (MQ=255)
               gcTGCATATCGTCAGCGTGGTCTGCTGGTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCg                                                                                                                                     <  1:4782099/151‑1 (MQ=255)
                    atatCGTCAGCGTGGTCTGCTGGTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGCGCCAGGACAGCCTCAGCCAGGAACGCTTCTTTACCATGGAGCGCAAGCTGTACCGCAGCCTCATGAACCCGGCgacgat                                                                                                                                >  2:1793160/1‑151 (MQ=255)
                      atCGTCAGCGTGGTCTGCTGGTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCg                                                                                                                              <  2:1002952/151‑1 (MQ=255)
                      atCGTCAGCGTGGTCTGCTGGTTCGCCGGCCTGTTCTACCCGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCg                                                                                                                              <  2:1122112/151‑1 (MQ=255)
                       tCGTCAGCGTGGTCTGCTGGTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCGGCATCATGAACCCGGCGATGATCGc                                                                                                                             >  2:4440214/1‑151 (MQ=255)
                            aGCGTGGTCTGCTGGTTCGCCGGCCTTTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCacat                                                                                                                        <  2:4754667/151‑1 (MQ=255)
                            aGCGTGGTCTGCTGGTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCacat                                                                                                                        >  1:5172621/1‑151 (MQ=255)
                             gCGTGGTCTGCTGGTTCGCCGGTCTGTTCTACCTGCCCAGGATGTTCGTCTACCACGCCCAGAGCGAGGACAGCATCAGCCAGGAACGCTTCGTTACCACGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCacata                                                                                                                       <  1:127628/151‑1 (MQ=255)
                              cGTGGTCTGCTGGTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCGGCATCATGAACCCGGCGATGATCGCCacatac                                                                                                                      >  2:202380/1‑151 (MQ=255)
                              cGTGGTCTGCTGGTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCacatac                                                                                                                      <  1:1839885/151‑1 (MQ=255)
                               gTGGTCTGCTGGTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCGGCATCATGAACCCGGCGATGATCGCCacataca                                                                                                                     <  1:379387/151‑1 (MQ=255)
                               gTGGTCTGCTGGTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCcacacaca                                                                                                                     >  1:607908/1‑151 (MQ=255)
                               gTGGTCTGCTGGTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCacataca                                                                                                                     >  1:5057266/1‑151 (MQ=255)
                               gTGGTCTGCTGGTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCacataca                                                                                                                     <  2:1981156/151‑1 (MQ=255)
                                tGGTCTGCTGGTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCacatacat                                                                                                                    <  2:192005/151‑1 (MQ=255)
                                  gTCTGCTGGTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACAttt                                                                                                                  <  1:1538462/151‑1 (MQ=255)
                                  gTCTGCTGGTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACAttt                                                                                                                  >  1:3923105/1‑151 (MQ=255)
                                   tCTGCTGGTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACAtttt                                                                                                                 <  2:2790437/151‑1 (MQ=255)
                                       gtgGTTCGCCGGCCTTTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCGGCATCAGGAACCCGGCGATGATCGCCACATACATTTTTGgc                                                                                                             <  2:4242545/150‑1 (MQ=255)
                                        tgGTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGgcg                                                                                                            <  2:1463285/151‑1 (MQ=255)
                                        tgGTGCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCCCGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGgcg                                                                                                            <  1:3601602/151‑1 (MQ=255)
                                         ggTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGgcgc                                                                                                           >  1:766869/1‑151 (MQ=255)
                                         ggTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGgcgc                                                                                                           <  1:1808535/151‑1 (MQ=255)
                                            tCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTg                                                                                                        <  2:1214424/151‑1 (MQ=255)
                                            tCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTg                                                                                                        >  2:4222528/1‑151 (MQ=255)
                                             cGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTgg                                                                                                       <  1:1478091/151‑1 (MQ=255)
                                                  gCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCt                                                                                                  >  1:1735390/1‑151 (MQ=255)
                                                    cTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCGGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCttt                                                                                                >  2:2241693/1‑149 (MQ=255)
                                                    cTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGt                                                                                                <  1:904188/151‑1 (MQ=255)
                                                      gTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTAc                                                                                              <  1:2043614/151‑1 (MQ=255)
                                                          tACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCa                                                                                          <  1:4407853/151‑1 (MQ=255)
                                                              tGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCAcccc                                                                                      <  1:444711/151‑1 (MQ=255)
                                                               gCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCAccccc                                                                                     >  1:3370605/1‑151 (MQ=255)
                                                               gCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCAccccc                                                                                     >  1:3369353/1‑151 (MQ=255)
                                                                 ccAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCgg                                                                                   <  1:3675973/151‑1 (MQ=255)
                                                                   aGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCACCggct                                                                                 >  2:5150904/1‑151 (MQ=255)
                                                                     gCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCggctgg                                                                               <  2:2381352/151‑1 (MQ=255)
                                                                     gCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTACGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCggctgg                                                                               <  2:5032648/151‑1 (MQ=255)
                                                                      cTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCggctggc                                                                              >  2:1830745/1‑151 (MQ=255)
                                                                      cTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCggctggc                                                                              <  1:4238191/151‑1 (MQ=255)
                                                                       tGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCggctggct                                                                             >  1:4039181/1‑151 (MQ=255)
                                                                            gTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGcc                                                                        >  2:1166382/1‑151 (MQ=255)
                                                                             tCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCGGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCa                                                                       >  1:1103684/1‑151 (MQ=255)
                                                                             tCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCa                                                                       >  1:1699484/1‑151 (MQ=255)
                                                                                   aCGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTg                                                                 >  1:2859221/1‑151 (MQ=255)
                                                                                      cccAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCt                                                              <  2:4347575/151‑1 (MQ=255)
                                                                                        cAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGc                                                            <  1:488591/151‑1 (MQ=255)
                                                                                         agagCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTGCCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTCGATGCAGTACCTCACCCCCGTCTGGCTGAGCCAGGGGTGGCTGcc                                                           >  1:3452765/1‑150 (MQ=255)
                                                                                          gtggcAGGACAGCATGAGCGTGGATCGCGTGGTTAACGTGGAGAGCAAGCTGAACCGCAGGATCATGAACCCGGCGATGATCGCCACATACATTTATGGCGCCTGGATGCTGTACCTAACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCAt                                                          <  2:681649/147‑1 (MQ=255)
                                                                                          gagCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCAt                                                          <  1:3174500/151‑1 (MQ=255)
                                                                                          gagCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTCCac                                                          >  2:3506939/1‑150 (MQ=255)
                                                                                              cAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCa                                                      >  1:2771696/1‑151 (MQ=255)
                                                                                              cAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCa                                                      <  1:192587/151‑1 (MQ=255)
                                                                                                ggACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAg                                                    >  1:4897477/1‑151 (MQ=255)
                                                                                                 gACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCGGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGc                                                   <  1:2704254/151‑1 (MQ=255)
                                                                                                  aCAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCt                                                  >  1:1575634/1‑151 (MQ=255)
                                                                                                  aCAGCATCAGCCAGGAACGCTTCGCTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCt                                                  >  2:2883759/1‑151 (MQ=255)
                                                                                                     gCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCCAGCTCAc                                               >  2:3011060/1‑151 (MQ=255)
                                                                                                     gCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCAc                                               <  2:959605/151‑1 (MQ=255)
                                                                                                     gCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCAc                                               <  2:2741266/151‑1 (MQ=255)
                                                                                                      cATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCAcc                                              >  1:3013043/1‑151 (MQ=255)
                                                                                                        tCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGCTGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCt                                            >  2:2142762/1‑151 (MQ=255)
                                                                                                          aGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTgg                                          >  2:2184702/1‑151 (MQ=255)
                                                                                                            ccAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTGGTc                                        <  2:4584852/151‑1 (MQ=255)
                                                                                                              aGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTGGTCAt                                      <  1:1385463/151‑1 (MQ=255)
                                                                                                                ggaCGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTGGTCATcc                                    <  1:2283780/149‑1 (MQ=255)
                                                                                                                gAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTGGTCATcc                                    <  2:1053008/151‑1 (MQ=255)
                                                                                                                 aaCGCATCGTTACAGTGGAGCGCAAGCTGTACCGAAGCATTATGTACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCGCCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTGGTCATCct                                   <  1:123154/151‑1 (MQ=255)
                                                                                                                  aCGCTTCGTTACCATGGAGCGCAAGCTGTACCGCGGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGCCTGCCTGCGCCAGCCGTGGCTGCATGCCCCCCTCACCCCTGTCATCctt                                  >  2:3896615/1‑150 (MQ=255)
                                                                                                                     cTTCGTTACCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTGGTCATCctgctg                               <  1:3979358/151‑1 (MQ=255)
                                                                                                                        cggtACCAGGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGAGGATCGCCACATACATATTTGGCGCCTGGATGCTGTACCTCGCCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTGGGCATCCTGATGAcc                            <  2:788363/148‑1 (MQ=255)
                                                                                                                          ttACCATGGAGCGCAAGCTGTCCCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTGGTCATCCTGCTGACCgg                          <  1:4337663/151‑1 (MQ=255)
                                                                                                                            aCCATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTGGTCATCCTGCTGACCGGCt                        >  2:3952722/1‑151 (MQ=255)
                                                                                                                             ccATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTGGTCATCCTGCTGACCGGCTa                       <  2:1428848/151‑1 (MQ=255)
                                                                                                                             ccATGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTGGTCATCCTGCTGACCGGCTa                       >  1:3275511/1‑151 (MQ=255)
                                                                                                                               aTGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTGGTCATCct                                   <  2:1518639/137‑1 (MQ=255)
                                                                                                                                tGGAGCGCAAGGTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTGGTCATCCTGCTGACCGGCTACCa                    <  2:2589769/151‑1 (MQ=255)
                                                                                                                                tGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTGGTCATCCTGCTGACCGGCTACCa                    >  1:2864762/1‑151 (MQ=255)
                                                                                                                                tGGAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTGGTCATCCTGCTGACCGGCTACCa                    >  2:3553659/1‑151 (MQ=255)
                                                                                                                                 ggAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTGGTCATCCTGCTGACCGGCTACCAt                   >  1:4769968/1‑151 (MQ=255)
                                                                                                                                 ggAGCGCAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTGGTCATCCTGCTGACCGGCTACCAt                   <  2:2293404/151‑1 (MQ=255)
                                                                                                                                      gcAAGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTGGTCATCCTGCTGACCGGCTACCATCACGt              >  2:4542839/1‑151 (MQ=255)
                                                                                                                                         aGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTGGTCATCCTGCTGACCGGCTACCATCACGTATg           >  1:2169838/1‑151 (MQ=255)
                                                                                                                                         aGCTGTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTGGTCATCCTGCTGACCGGCTACCATCACGTATg           >  1:677838/1‑151 (MQ=255)
                                                                                                                                          gCTGTACGGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTGGTCATCCTGCTGACCGGCTACCATCACGTATGc          <  2:3216284/151‑1 (MQ=255)
                                                                                                                                             gTACCGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTGGCCATCCTGCTGACCGGCTACCATCACGTATGCgct       >  2:5196941/1‑149 (MQ=255)
                                                                                                                                                ccGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTGGTCATCCTGCTGACCGGCTACCATCACGTATGCGGTGcc    <  2:4065576/151‑1 (MQ=255)
                                                                                                                                                ccGCAGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTGGTCATCCTGCTGACCGGCTACCATCACGTATGCGGTGcc    <  1:938253/151‑1 (MQ=255)
                                                                                                                                                 cgcaGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTTGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTGGTCATCCTGCTGACCGGCTACCATCACGTATGCGGTGccc   >  1:3496718/1‑151 (MQ=255)
                                                                                                                                                  gcgGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTGGTCATCCTGCTGACCGGCTACCATCACGTATGCGGTGCCCa  >  2:4149639/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCTATGGATCAAAGCGCTGCATATCGTCAGCGTGGTCTGCTGGTTCGCCGGCCTGTTCTACCTGCCCAGGCTGTTCGTCTACCACGCCCAGAGCCAGGACAGCATCAGCCAGGAACGCTTCGTTACCATGGAGCGCAAGCTGTACCGCGGCATCATGAACCCGGCGATGATCGCCACATACATTTTTGGCGCCTGGATGCTGTACCTCACCCCCGGCTGGCTGAGCCAGGGGTGGCTGCATGCCAAGCTCACCCTGGTCATCCTGCTGACCGGCTACCATCACGTATGCGGTGCCCA  >  CP009974/1586267‑1586563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑