Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 1,611,819 C→T G121S (GGC→AGC)  RPPX_07240 ← aminoglycoside phosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099741,611,8190CT98.1% 942.6 / 1.6 267G121S (GGC→AGC) RPPX_07240aminoglycoside phosphotransferase
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base T (138/122);  total (142/125)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

Only 100 of 267 total aligned reads displayed.

AGGCTCAGGCCCAGCTCACGCCCCACGTACCATTCGGGGAACAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCCATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCAGCGCTGGCCAGCAGGTGGTCGATGGCGACGAACGG  >  CP009974/1611671‑1611966
                                                                                                                                                    |                                                                                                                                                   
 ggCTCAGGCCCAGCTCACGCCCCACGTACCATTCGGGGAACAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGCACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATc                                                                                                                                                  >  2:3688093/1‑151 (MQ=255)
 ggCTCAGGCCCAGCTCACGCCCCACGTACCATTCGGGGAACAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATc                                                                                                                                                  <  1:2697047/151‑1 (MQ=255)
 ggCTCAGGCCCAGCTCACGCCCCACGTACCATTCGGGGAACAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATc                                                                                                                                                  <  1:247336/151‑1 (MQ=255)
 ggCTCAGGCCCAGCTCACGCCCCACGTACCATTCGGGGAACAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATc                                                                                                                                                  >  2:3867529/1‑151 (MQ=255)
  gCTCAGGCCCAGCTCACGCCCCACGTACCATTCGGGGAACAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCg                                                                                                                                                 <  2:1210908/151‑1 (MQ=255)
  gCTCAGGCCCAGCTCACGCCCCACGTACCATTCGGGGAACAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACCTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCg                                                                                                                                                 >  2:3962393/1‑151 (MQ=255)
   cTCAGGCCCAGCTCACGCCCCACGTACCATTCGGGGAACAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGAGGTCGGCGGCGAACAGGCTATCgg                                                                                                                                                >  2:1034181/1‑151 (MQ=255)
   cTCAGGCCACACTCACGCCCCACGTACCAGTCGGGGAACAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAACGGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCgg                                                                                                                                                <  2:440208/151‑1 (MQ=255)
     cAGGCCCAGCTCACGCCCCACGTACCATTCGGGGAACAGTTCCACTTCACGGCGCAGCAGGTCGTCGGCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCgggg                                                                                                                                              >  2:2087531/1‑149 (MQ=255)
     cAGGCCCAGCTCACGCCCCACGTACCATTCGGGGAACAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCg                                                                                                                                              >  1:702586/1‑151 (MQ=255)
      aGGCCCAGCTCACGCCCCACGTACCATTCGGGGAACAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGt                                                                                                                                             >  2:400905/1‑151 (MQ=255)
           cAGCTCACGCCCCACGTACCATTCGGGGAACAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCg                                                                                                                                        <  1:3101479/151‑1 (MQ=255)
              cTCAAGCCACACGTACCATTCGGGGAACAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCCGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCa                                                                                                                                     <  2:488079/151‑1 (MQ=255)
                cACGCCCCACGTACCATTCGGGGAACAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCAtt                                                                                                                                   <  1:2365033/151‑1 (MQ=255)
                 aCGCCCCACGTACCATTCGGGGAACAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATtg                                                                                                                                  <  1:1509801/151‑1 (MQ=255)
                   ggtcgaCGGACCAGTGGGGGAACAGTTCCACTGCACGGCGCCGCAGGGCGTCGTCGTAGCTGGGCGGCGGCGCGTCCATCGGCCAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATtgat                                                                                                                                <  2:4729118/146‑1 (MQ=255)
                      ccACGTACCATTCGGGGAACAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTCCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTgatgat                                                                                                                             >  2:711743/1‑151 (MQ=255)
                         cGTACCATTCGGGGAACAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTc                                                                                                                          >  2:4222471/1‑151 (MQ=255)
                          gTACCATTCGGGGAACAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCa                                                                                                                         <  1:4553140/151‑1 (MQ=255)
                           tACCATTCGGGGAACAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCaa                                                                                                                        <  1:1041814/151‑1 (MQ=255)
                            aCCATTCAGGGAACAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGAATTGATGATGTCAAg                                                                                                                       <  2:3222737/151‑1 (MQ=255)
                            aCCATGCGGGGAACAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAg                                                                                                                       <  2:644997/151‑1 (MQ=255)
                                  cGGGGAACAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGGACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGt                                                                                                                 >  2:2002587/1‑151 (MQ=255)
                                  cGGGGAACAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGt                                                                                                                 <  2:3648188/151‑1 (MQ=255)
                                  cGGGGAACAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGt                                                                                                                 <  1:1724239/151‑1 (MQ=255)
                                  cGGGGAACAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGt                                                                                                                 >  1:3694885/1‑151 (MQ=255)
                                   ggggAACAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTc                                                                                                                >  1:21745/1‑151 (MQ=255)
                                      gAATAGCTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGa                                                                                                             <  1:258664/151‑1 (MQ=255)
                                      gAACGGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGa                                                                                                             <  2:2867136/151‑1 (MQ=255)
                                      gAACAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATtga                                                                                                                                 <  1:3927305/131‑1 (MQ=255)
                                       aaCAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAg                                                                                                            <  1:2760161/151‑1 (MQ=255)
                                        aCAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGt                                                                                                           <  2:1839198/151‑1 (MQ=255)
                                          aGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGc                                                                                                         <  2:2913015/151‑1 (MQ=255)
                                           gTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGcc                                                                                                        >  2:2447660/1‑151 (MQ=255)
                                               cACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGGCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAgg                                                                                                    >  2:3852547/1‑150 (MQ=255)
                                                aCTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGAt                                                                                                   <  2:1559295/151‑1 (MQ=255)
                                                 cTTCACGACGCAGCGGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATc                                                                                                  <  2:3203999/151‑1 (MQ=255)
                                                        gggcagcaGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAagc                                                                                           <  2:1403448/149‑1 (MQ=255)
                                                         cgcAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAAGAGGCTATCGGCGTCGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAagca                                                                                          >  2:5021268/1‑151 (MQ=255)
                                                               agGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCaaaa                                                                                    >  2:3518956/1‑151 (MQ=255)
                                                               agGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCaaaa                                                                                    >  2:561485/1‑151 (MQ=255)
                                                               agGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCaaaa                                                                                    >  1:4591831/1‑151 (MQ=255)
                                                                gggCGTCGTCGCAGCTGGGCAGCGGCGCGCCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCCGCGAACAGGCTATCGCCGTTGTCGGCATTGATGATGTCAAGGTATGCCCGAGCGCCCAGATCCCCAAGCAGCacaaa                                                                                   >  2:4791359/1‑151 (MQ=255)
                                                                  gCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGc                                                                                 >  1:4777820/1‑151 (MQ=255)
                                                                    gtcgtcgtAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCa                                                                               >  2:2109626/1‑151 (MQ=255)
                                                                    gtcgtcgtAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCa                                                                               >  2:4510960/1‑151 (MQ=255)
                                                                         cgtAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTc                                                                          <  2:361744/151‑1 (MQ=255)
                                                                         cgtAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTc                                                                          >  1:3642513/1‑151 (MQ=255)
                                                                          gtAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTcc                                                                         >  1:1257707/1‑151 (MQ=255)
                                                                          gtAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGCCGGCATCGACGACGCCAAGGCATGTCCGAGTGCCCAGACCCCCACGCACCACACACCCACgcccc                                                                         >  2:4492400/1‑147 (MQ=255)
                                                                               tGGGCCGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCCTCGATGGCGTCCGCGAACAGGCTATCGCCGTCGTCGGCACTGCAGATGCCAAGGTCTGTCTGAGCGCCCAGATCCCCAAGCAGCCAAACGCCACGTTCCAGGTc                                                                    >  2:4610084/1‑151 (MQ=255)
                                                                                gggCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTcc                                                                   <  1:2968487/151‑1 (MQ=255)
                                                                                  gCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCAAAAAAAAAAAAAAAGCCACGTTCCAGGTCCTg                                                                 <  2:748204/151‑1 (MQ=255)
                                                                                   cAGCGGCGCGTCCATAGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGCACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTgg                                                                <  2:767483/151‑1 (MQ=255)
                                                                                    aGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTggg                                                               >  2:3353743/1‑151 (MQ=255)
                                                                                    aGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTggg                                                               >  1:4285268/1‑151 (MQ=255)
                                                                                      cggcgCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCg                                                             >  1:4846181/1‑151 (MQ=255)
                                                                                          gcgTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGa                                                         >  1:1829299/1‑151 (MQ=255)
                                                                                           cgTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGAt                                                        >  2:2222943/1‑151 (MQ=255)
                                                                                             tCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCa                                                      <  2:3307130/151‑1 (MQ=255)
                                                                                             tCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCa                                                      <  1:3319669/151‑1 (MQ=255)
                                                                                             tCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCa                                                      <  1:2919305/151‑1 (MQ=255)
                                                                                               cATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGc                                                    >  1:3371156/1‑151 (MQ=255)
                                                                                                    gCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGcac                                               <  1:1442501/151‑1 (MQ=255)
                                                                                                      aaaCGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACAt                                             >  2:1947025/1‑151 (MQ=255)
                                                                                                      aaaCGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACAt                                             >  1:1842033/1‑151 (MQ=255)
                                                                                                       aaCGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATg                                            >  1:4084829/1‑151 (MQ=255)
                                                                                                         cGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTa                                          <  2:4045589/151‑1 (MQ=255)
                                                                                                          gCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTAc                                         >  1:4440144/1‑151 (MQ=255)
                                                                                                          gCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTAc                                         >  2:2018554/1‑151 (MQ=255)
                                                                                                            tGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGt                                       >  2:5197574/1‑151 (MQ=255)
                                                                                                              gAACTTGAGAAGGGGATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCa                                     <  2:4230401/151‑1 (MQ=255)
                                                                                                              gAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCa                                     <  1:5023662/151‑1 (MQ=255)
                                                                                                               aaCTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCAg                                    <  1:141647/151‑1 (MQ=255)
                                                                                                               aaCTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCAg                                    >  1:1716113/1‑151 (MQ=255)
                                                                                                                 cTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCAgcg                                  >  1:313607/1‑151 (MQ=255)
                                                                                                                    cAGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCAGCGCTg                               >  1:2243765/1‑151 (MQ=255)
                                                                                                                     aGAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCAGCGCTgg                              <  1:3851466/151‑1 (MQ=255)
                                                                                                                      gAAGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCAGCGCTGGc                             <  1:514209/151‑1 (MQ=255)
                                                                                                                       aaGGGCATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGAGGCACATGTACGTCACCGCTGGcc                            >  2:2988174/1‑151 (MQ=255)
                                                                                                                            cATCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCAGCGCTGGCcagcag                       <  1:4725511/151‑1 (MQ=255)
                                                                                                                             aTCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCAGCGCTGGCCAGCAgg                      >  1:4405480/1‑151 (MQ=255)
                                                                                                                             aTCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCAGCGCTGGCCAGCAgg                      <  1:3132468/151‑1 (MQ=255)
                                                                                                                             aTCGATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCAGCGCTGGCCAGCAgg                      >  1:879267/1‑151 (MQ=255)
                                                                                                                                gATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCAGCGCTGGCCAGCAggtgg                   >  1:1166090/1‑151 (MQ=255)
                                                                                                                                gATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCAGCGCTGGCCAGCAggtgg                   >  1:3489848/1‑151 (MQ=255)
                                                                                                                                gATGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCAGCGCTGGCCAGCAggtgg                   >  1:5053554/1‑151 (MQ=255)
                                                                                                                                 aTGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCAGCGCTGGCCAGCAggtggt                  >  1:3218386/1‑151 (MQ=255)
                                                                                                                                 aTGGCGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCAGCGCTGGCCAGCAggtggt                  >  2:1171283/1‑151 (MQ=255)
                                                                                                                                     cGTCGGCGGACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCAGCGCTGGCCAGCAGGTGGTCGAt              <  1:2002587/151‑1 (MQ=255)
                                                                                                                                     cGTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCAGCGCTGGCCAGCAGGTGGTCGAt              >  1:2671873/1‑151 (MQ=255)
                                                                                                                                      gTCGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCAGCGCTGGCCAGCAGGTGGTCGATg             >  1:1650721/1‑151 (MQ=255)
                                                                                                                                        cGGCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCAGCGCTGGCCAGCAGGTGGTCGATGGc           >  1:2327135/1‑151 (MQ=255)
                                                                                                                                         ggCGAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCAGCGCTGGCCAGCAGGTGGTCGATGGcg          <  1:4955287/151‑1 (MQ=255)
                                                                                                                                            gAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCAGCGCTGGCCAGCAGGTGGTCGATGGcgacg       >  2:2751914/1‑151 (MQ=255)
                                                                                                                                            gAACAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCAGCGCTGGCCAGCAGGTGGTCGATGGcgacg       >  2:2587482/1‑151 (MQ=255)
                                                                                                                                               cAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCAGCGCTGGCCAGCAGGTGGTCGATGGCGACGAAc    <  2:373914/151‑1 (MQ=255)
                                                                                                                                               cAGGCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCAGCGCTGGCCAGCAGGTGGTCGATGGCGACGAAc    <  1:3886924/151‑1 (MQ=255)
                                                                                                                                                aGGCCATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCCGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCAGCGCTGGCCAGCAGGTGGTCGATGGCGACGAACg   >  2:3115559/1‑151 (MQ=255)
                                                                                                                                                 ggCTATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCAGCGCTGGCCAGCAGGTGGTCGATGGCGACGAACgg  <  2:1724033/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AGGCTCAGGCCCAGCTCACGCCCCACGTACCATTCGGGGAACAGTTCCACTTCACGGCGCAGCAGGGCGTCGTCGTAGCTGGGCAGCGGCGCGTCCATCGGCAAACGCTGGAACTTCAGAAGGGCATCGATGGCGTCGGCGAACAGGCCATCGGCGTTGTCGGCATTGATGATGTCAAGGTATGTCTGAGTGCCCAGATCCCCAAGCAGCAAAAAGCCACGTTCCAGGTCCTGGGCGTGGATCAGCGGCACATGTACGTCAGCGCTGGCCAGCAGGTGGTCGATGGCGACGAACGG  >  CP009974/1611671‑1611966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑