Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 1,639,093 G→A A18T (GCC→ACC)  RPPX_07350 → AsnC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099741,639,0930GA99.6% 986.9 / NA 262A18T (GCC→ACC) RPPX_07350AsnC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (125/135);  total (125/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 262 total aligned reads displayed.

TGCCAAGCCGGCGCGTCAACGGTGATCGACTGGACCTGGCCTTGCCCTGATTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCGCCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCGGCTATGGCGTGCGCCTGGGCCAGAGTGTGA  >  CP009974/1638946‑1639240
                                                                                                                                                   |                                                                                                                                                   
 gCCAAGCCGGCGCGTCAACGGTGATCGACTGGACCTGGCCTTGCCCTGATTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCct                                                                                                                                                 <  2:859443/151‑1 (MQ=255)
 gCCAAGCCGGCGCGTCAACGGTGATCGACTGGACCTGGCCTTGCCCTGATTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCct                                                                                                                                                 <  1:766327/151‑1 (MQ=255)
 gCCAAGCCGGCGCGTCAACGGTGATCGACTGGACCTGGCCTTGCCCTGATTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCct                                                                                                                                                 <  2:3954024/151‑1 (MQ=255)
     aGCCGGCGCGTCAACGGTGATCGACTGGACCTGGCCTTGCCCTGATTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCctgctg                                                                                                                                             >  1:1313386/1‑151 (MQ=255)
        cGGCGCGTCAACGGTGATCGACTGGACCTGGCCTTGCCCTGATTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCCCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAg                                                                                                                                          >  2:5011790/1‑151 (MQ=255)
         ggCGCGTCAACGGTGATCGACTGGACCTGGCCTTGCCCTGATTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGa                                                                                                                                         >  1:4926951/1‑151 (MQ=255)
         ggCGCGTCAACGGTGATCGACTGGACCTGGCCTTGCCCTGATTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGa                                                                                                                                         >  1:2527100/1‑151 (MQ=255)
          gcgcGTCAACGGTGATCGACTGGACCTGGCCTTGCCCTGATTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGAt                                                                                                                                        >  2:3695821/1‑151 (MQ=255)
           cgcgTCAACGGTGATCGACTGGACCTGGCCTTGCCCTGATTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATc                                                                                                                                       <  2:924672/151‑1 (MQ=255)
            gcgTCAACGGTGATCGACTGGACCTGGCCTTGCCCTGATTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCa                                                                                                                                      >  1:3359003/1‑151 (MQ=255)
             cgTCAACGGTGATCGACTGGACCTGGCCTTGCCCTGATTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCaa                                                                                                                                     >  2:3315558/1‑151 (MQ=255)
              gTCAACGGTGATCGACTGGACCTGGCCTTGCCCTGATTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAAc                                                                                                                                    <  1:3691564/151‑1 (MQ=255)
               tCAACGGTGATCGACTGGACCTGGCCTTGCCCTGATTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACg                                                                                                                                   <  1:1804373/151‑1 (MQ=255)
                cAACGGTGATCGACTGGACCTGGCCTTGCCCTGATTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGc                                                                                                                                  <  1:262634/151‑1 (MQ=255)
                      tGATCGACTGGACCTGGCCTTGCCCTGATTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGa                                                                                                                            >  2:3361134/1‑151 (MQ=255)
                      tGATCGACTGGACCTGGCCTTGCCCTGATTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGa                                                                                                                            <  2:1775732/151‑1 (MQ=255)
                             cTGGACCTGGCCTTGCCCTGATTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTc                                                                                                                     <  2:480708/151‑1 (MQ=255)
                               ggACCTGGCCTTGCCCTGATTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGc                                                                                                                   >  2:686782/1‑151 (MQ=255)
                                 aCCTGGCCTTGCCCTGATTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCa                                                                                                                 <  1:4210816/151‑1 (MQ=255)
                                     ggCCTTGCCCTGATTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCt                                                                                                             <  2:1197693/151‑1 (MQ=255)
                                        cTTGCCCTGATTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTcgc                                                                                                          >  2:1616616/1‑151 (MQ=255)
                                        cTTGCCCTGATTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTACAGATCAACGCCCGCGAAAGCGTCGCCACCCTcgc                                                                                                          >  2:3128168/1‑151 (MQ=255)
                                          tGCCCTGATTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTcgccc                                                                                                        <  2:4313111/151‑1 (MQ=255)
                                          tGCCCTGATTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTcgccc                                                                                                        <  1:85902/151‑1 (MQ=255)
                                          tGCCCTGATTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTcgccc                                                                                                        <  1:4062668/151‑1 (MQ=255)
                                          tGCCCTGATTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTcgccc                                                                                                        <  1:91219/151‑1 (MQ=255)
                                                 aTTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCt                                                                                                 >  1:2253108/1‑151 (MQ=255)
                                                 aTTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCt                                                                                                 <  1:20238/151‑1 (MQ=255)
                                                   tGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTgg                                                                                               >  2:4875709/1‑151 (MQ=255)
                                                    ggCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTggg                                                                                              >  1:1076091/1‑151 (MQ=255)
                                                     gCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGc                                                                                             <  2:1414591/151‑1 (MQ=255)
                                                       acaGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCac                                                                                           >  2:2844369/1‑150 (MQ=255)
                                                       acaGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCAt                                                                                           >  2:4137420/1‑151 (MQ=255)
                                                       acaGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCAt                                                                                           <  1:1644849/151‑1 (MQ=255)
                                                         aGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCg                                                                                         >  2:4595800/1‑151 (MQ=255)
                                                         aGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCg                                                                                         <  1:5086899/151‑1 (MQ=255)
                                                            ctggctAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGccc                                                                                      <  1:1058288/151‑1 (MQ=255)
                                                             tggctAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCg                                                                                     >  1:5003275/1‑151 (MQ=255)
                                                                  aggctagCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTa                                                                                <  1:719729/151‑1 (MQ=255)
                                                                    gctagCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTAcc                                                                              <  1:3753609/151‑1 (MQ=255)
                                                                      tagCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCgg                                                                            >  1:4839322/1‑150 (MQ=255)
                                                                         cGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCaa                                                                         >  2:1286032/1‑151 (MQ=255)
                                                                         cGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCaa                                                                         >  1:619381/1‑151 (MQ=255)
                                                                          gggCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAAt                                                                        >  2:4046268/1‑151 (MQ=255)
                                                                           ggCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAAtt                                                                       >  2:3659259/1‑151 (MQ=255)
                                                                                 aCGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGcc                                                                 >  2:3996392/1‑151 (MQ=255)
                                                                                    tAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTgg                                                              >  2:5128164/1‑151 (MQ=255)
                                                                                     agagACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGa                                                             >  1:985552/1‑151 (MQ=255)
                                                                                     agagACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGa                                                             <  1:3150220/151‑1 (MQ=255)
                                                                                     agagACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGa                                                             >  2:2979126/1‑151 (MQ=255)
                                                                                      gagaCTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAg                                                            >  1:1739092/1‑151 (MQ=255)
                                                                                       agaCTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGc                                                           <  1:1252142/151‑1 (MQ=255)
                                                                                       agaCTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGc                                                           <  2:577253/151‑1 (MQ=255)
                                                                                        gaCTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCg                                                          <  1:1390688/151‑1 (MQ=255)
                                                                                        gaCTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCg                                                          >  2:4743070/1‑151 (MQ=255)
                                                                                          cTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGc                                                        >  1:3703888/1‑151 (MQ=255)
                                                                                          cTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGc                                                        >  1:2157382/1‑151 (MQ=255)
                                                                                          atCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGACATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGc                                                        <  2:119436/150‑1 (MQ=255)
                                                                                            cGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTg                                                      <  2:3399185/151‑1 (MQ=255)
                                                                                              ccATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGga                                                    <  2:612589/151‑1 (MQ=255)
                                                                                                aTGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGgaga                                                  <  2:208142/151‑1 (MQ=255)
                                                                                                   tCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGaaaa                                               <  2:5058001/151‑1 (MQ=255)
                                                                                                     ggATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAAc                                             >  1:2760306/1‑151 (MQ=255)
                                                                                                      gATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACa                                            >  2:2459061/1‑151 (MQ=255)
                                                                                                       aTTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACaa                                           <  1:1879727/151‑1 (MQ=255)
                                                                                                        ttCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAg                                          >  1:2607500/1‑151 (MQ=255)
                                                                                                           ccGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGtt                                       <  1:1926757/151‑1 (MQ=255)
                                                                                                              cTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCTACTGTAGCGGCTGGAGAAAAACACGGCTAtc                                    >  2:3611332/1‑151 (MQ=255)
                                                                                                               tCCATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTAtcc                                   >  2:1523823/1‑150 (MQ=255)
                                                                                                                ccATTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTAtctc                                  <  1:3629064/151‑1 (MQ=255)
                                                                                                                  tttACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCg                                <  2:1353769/150‑1 (MQ=255)
                                                                                                                  aTTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCg                                <  1:2534109/151‑1 (MQ=255)
                                                                                                                  aTTACGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCCCCg                                >  2:4381750/1‑151 (MQ=255)
                                                                                                                     aCGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCGGCt                             <  2:4916096/151‑1 (MQ=255)
                                                                                                                     aCGCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCGGCt                             >  2:4654752/1‑151 (MQ=255)
                                                                                                                       gCTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATTTCCGGCTAt                           >  1:4702015/1‑151 (MQ=255)
                                                                                                                        cTGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCGGCTATg                          >  1:3299148/1‑151 (MQ=255)
                                                                                                                        cTGGATGAAATCGATCGCCAGCTGAACACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCGGCTATg                          <  1:2907659/151‑1 (MQ=255)
                                                                                                                         tGGATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCGGCTATgg                         >  2:4954229/1‑151 (MQ=255)
                                                                                                                          ggATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCGGCTATGGc                        <  1:412135/151‑1 (MQ=255)
                                                                                                                           gATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCGGCTATGGCg                       >  1:3812325/1‑151 (MQ=255)
                                                                                                                           gATGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCGGCTATGGCg                       <  1:4970219/151‑1 (MQ=255)
                                                                                                                            aTGAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCGGCTATGGCGt                      >  1:4821280/1‑151 (MQ=255)
                                                                                                                              gAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCGGCTATGGCGTgc                    <  2:837434/151‑1 (MQ=255)
                                                                                                                              gAAATCGATCGCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCGGCTATGGCGTgc                    >  2:2056092/1‑151 (MQ=255)
                                                                                                                                   cgatcgCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCGGCTATGGCGTGCGCCTg               <  2:3532062/151‑1 (MQ=255)
                                                                                                                                   cgatcgCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCGGCTATGGCGTGCGCCTg               <  1:2334315/151‑1 (MQ=255)
                                                                                                                                   cgatcgCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCGGCTATGGCGTGCGCCTg               <  1:1594986/151‑1 (MQ=255)
                                                                                                                                    gatcgCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCGGCTATGGCGTGCGCCTgg              <  2:455783/151‑1 (MQ=255)
                                                                                                                                    gatcgCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCGGCTATGGCGTGCGCCCgg              >  2:3790733/1‑151 (MQ=255)
                                                                                                                                      tcgCCAGCTGATCACCCTGCTGCAGATCAACGCCCGAGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGTACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAGAAACAGGGTTATCTCCGGCTATGGCGTGCGCCTGGGc            >  2:4096966/1‑151 (MQ=255)
                                                                                                                                       cgCCAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCGGCTATGGCGTGCGCCTGGGcc           >  1:3992695/1‑151 (MQ=255)
                                                                                                                                         ccAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCGGCTATGGCGTGCGCCTGGGCCag         >  1:2929949/1‑151 (MQ=255)
                                                                                                                                          cAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCGGCTATGGCGTGCGCCTGGGCCaga        <  2:2613211/151‑1 (MQ=255)
                                                                                                                                          cAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCGGCTATGGCGTGCGCCTGGGCCaga        <  2:3067865/151‑1 (MQ=255)
                                                                                                                                          cAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCGGCTATGGCGTGCGCCTGGGCCaga        >  1:2619411/1‑151 (MQ=255)
                                                                                                                                          cAGCTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCGGCTATGGCGTGCGCCTGGGCCaga        >  1:3168433/1‑151 (MQ=255)
                                                                                                                                             cTGATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCGGCTATGGCGTGCGCCTGGGCCAGAgtg     >  1:3485858/1‑151 (MQ=255)
                                                                                                                                               gATCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCGGCTATGGCGTGCGCCTGGGCCAGAGtgtg   <  1:4743070/151‑1 (MQ=255)
                                                                                                                                                aTCACCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCGGCTATGGCGTGCGCCTGGGCCAGAGTGTGa  >  1:5124548/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGCCAAGCCGGCGCGTCAACGGTGATCGACTGGACCTGGCCTTGCCCTGATTGGCACAGGCTGGCTAGGCTAGCGGGCCAAACGTAGAGACTCGCCATGTCGGATTCCCGCTCCATTACGCTGGATGAAATCGATCGCCAGCTGATCGCCCTGCTGCAGATCAACGCCCGCGAAAGCGTCGCCACCCTCGCCCGCCAGCTGGGCATCGCCCGCACTACCGTCAATTCGCGCCTGGAGCGGCTGGAGAAAAACAAGGTTATCTCCGGCTATGGCGTGCGCCTGGGCCAGAGTGTGA  >  CP009974/1638946‑1639240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑