Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 1,654,943 G→A A22A (GCC→GCT)  RPPX_07410 ← acyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099741,654,9430GA99.6% 988.9 / NA 260A22A (GCC→GCT) RPPX_07410acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (111/148);  total (112/148)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 260 total aligned reads displayed.

CGTTGTCCCAGTGGCAGCCCTCTTCGTCGGCGGCGCGGCTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCGGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGAACCGCGACACTGGGGTTACTGGCAGTGTAACCGAACAACTTTT  >  CP009974/1654795‑1655090
                                                                                                                                                    |                                                                                                                                                   
   tGTCCCAGTGGCAGCCCTCTTCGTCGGCGGCGCGGCTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCAc                                                                                                                                                <  1:1064338/151‑1 (MQ=255)
   tGTCCCAGTGGCAGCCCTCTTCGTCGGCGGCGCGGCTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCAc                                                                                                                                                >  2:2524855/1‑151 (MQ=255)
    gTCCCAGTGGCAGCCCTCTTCGTCGGCGGCGCGGCTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACg                                                                                                                                               <  1:3182298/151‑1 (MQ=255)
     tCCCAGTGGCAGCCCTCTTCGTCGGCGGCGCGGCTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGt                                                                                                                                              >  1:5143073/1‑151 (MQ=255)
      cccAGTGGCAGCCCTCTTCGTCGGCGGCGCGGCTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGtt                                                                                                                                             <  2:4887465/151‑1 (MQ=255)
      cccAGTGGCAGCCCTCTTCGTCGGCGGCGCGGCTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGtt                                                                                                                                             >  1:1438856/1‑151 (MQ=255)
           tGGCAGCCCTCTTCGTCGGCGGCGCGGCTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTCAAGa                                                                                                                                        >  1:2547886/1‑151 (MQ=255)
               aGCCCTCTTCGTCGGCGGCGCGGCTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACttc                                                                                                                                    <  1:4484084/151‑1 (MQ=255)
                 cccTCTTCGTCGGCGGCGCGGCTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACttcat                                                                                                                                  >  2:3402451/1‑151 (MQ=255)
                 cccTCTTCGTCGGCGGCGCGGCTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACttcat                                                                                                                                  >  1:4504649/1‑151 (MQ=255)
                  ccTCTTCGTCGGCGGCGCGGCTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACttcatt                                                                                                                                 <  2:4133976/151‑1 (MQ=255)
                    tctTCGTCGGCGGCGCGGCTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACttcattca                                                                                                                               <  2:1930997/151‑1 (MQ=255)
                     ctTCGTCGGCGGCGCGGCTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAg                                                                                                                              <  1:2200300/151‑1 (MQ=255)
                       tcgtcgGCGGCGCGGCTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTa                                                                                                                            <  2:1634676/151‑1 (MQ=255)
                       tcgtcgGCGGCGCGGCTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTa                                                                                                                            >  1:4634650/1‑151 (MQ=255)
                        cgtcgGCGGCGCGGCTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTAc                                                                                                                           <  1:2871314/151‑1 (MQ=255)
                         gtcgGCGGCGCGGCTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCACGGGCAACTGCGTCCACTGCTCAGCCACGTTGACGACTTCATTCAGTACg                                                                                                                          >  2:5037754/1‑151 (MQ=255)
                          tcgGCGGCGCGGCTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAGCTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGa                                                                                                                         <  1:3947516/151‑1 (MQ=255)
                          tcgGCGGCGCGGCTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGa                                                                                                                         <  2:1331328/151‑1 (MQ=255)
                           cggcggCGCGGCTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGaa                                                                                                                        >  1:4942682/1‑151 (MQ=255)
                           cggcggCGCGGCTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGaa                                                                                                                        <  2:3135426/151‑1 (MQ=255)
                            ggcggcGCGGCTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAg                                                                                                                       >  2:1827897/1‑151 (MQ=255)
                             gcggcgcggcTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAgc                                                                                                                      <  1:487614/151‑1 (MQ=255)
                              cggcgcggcTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAgcg                                                                                                                     <  2:941202/151‑1 (MQ=255)
                               ggcgcggcTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAgcgc                                                                                                                    >  1:5008736/1‑151 (MQ=255)
                                gcgcggcTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCa                                                                                                                   <  1:1250158/151‑1 (MQ=255)
                                 cgcggcTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCTTCCACTGCGCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCan                                                                                                                  <  1:200713/151‑2 (MQ=255)
                                 cgcggcTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCAt                                                                                                                  >  1:3448882/1‑151 (MQ=255)
                                   cggcTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGt                                                                                                                <  1:4877057/151‑1 (MQ=255)
                                    ggcTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTc                                                                                                               <  1:3636623/151‑1 (MQ=255)
                                     gcTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTcg                                                                                                              >  2:2423794/1‑151 (MQ=255)
                                        gAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCgcgc                                                                                                           >  1:3476099/1‑151 (MQ=255)
                                        gAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCgcgc                                                                                                           >  2:149858/1‑151 (MQ=255)
                                         aGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCa                                                                                                          <  1:4164574/151‑1 (MQ=255)
                                         aGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCa                                                                                                          <  2:2207988/151‑1 (MQ=255)
                                           cggcgCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAgg                                                                                                        <  1:4325068/151‑1 (MQ=255)
                                            ggcgCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCGGCCACGTTGAAGACTTCATTCAGTACCAAGCGCATGTCGCGCAggg                                                                                                       >  2:1034455/1‑151 (MQ=255)
                                            ggcgCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAggg                                                                                                       <  1:1569124/151‑1 (MQ=255)
                                             gcgcAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAgggg                                                                                                      >  2:5221269/1‑151 (MQ=255)
                                              cgcAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGgc                                                                                                     >  2:2838022/1‑151 (MQ=255)
                                                cAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGgcgc                                                                                                   <  1:977355/151‑1 (MQ=255)
                                                 aaTGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCt                                                                                                  <  2:1279185/151‑1 (MQ=255)
                                                    ggTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTta                                                                                               >  2:2730355/1‑151 (MQ=255)
                                                    ggTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTta                                                                                               <  2:3998394/151‑1 (MQ=255)
                                                     gTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTtat                                                                                              <  2:2442957/151‑1 (MQ=255)
                                                       ccTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAg                                                                                            <  1:1875743/151‑1 (MQ=255)
                                                         tGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTc                                                                                          <  1:1139185/151‑1 (MQ=255)
                                                           gCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAg                                                                                        <  2:1068408/151‑1 (MQ=255)
                                                             ggTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGcc                                                                                      <  2:4462508/151‑1 (MQ=255)
                                                             ggTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGcc                                                                                      >  2:4936908/1‑151 (MQ=255)
                                                              gTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCa                                                                                     <  2:3471509/151‑1 (MQ=255)
                                                               ttACCTTGCCGGCTTCTTCCCGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCAt                                                                                    <  2:3565346/151‑1 (MQ=255)
                                                                  ccTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCCGGGGCGCTTTATAGTCAGCCATGAc                                                                                 >  2:4191273/1‑151 (MQ=255)
                                                                  ccTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGAc                                                                                 <  1:1358872/151‑1 (MQ=255)
                                                                  ccTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGAc                                                                                 >  1:2706778/1‑151 (MQ=255)
                                                                  ccTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGAc                                                                                 <  1:4939108/151‑1 (MQ=255)
                                                                  ccTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTCCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTGGAGAATCAGCCATgga                                                                                 >  2:1344284/1‑149 (MQ=255)
                                                                   cTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACa                                                                                <  2:3050769/151‑1 (MQ=255)
                                                                    ttGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACaa                                                                               >  1:2669427/1‑151 (MQ=255)
                                                                            ttcttcCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGc                                                                       >  2:3942185/1‑151 (MQ=255)
                                                                            ttcttcCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGc                                                                       >  2:375676/1‑151 (MQ=255)
                                                                              cttcCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTa                                                                     <  1:179961/151‑1 (MQ=255)
                                                                              cttcCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTa                                                                     >  1:935634/1‑151 (MQ=255)
                                                                                    gCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTACTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAgtcggg                                                               >  1:3550052/1‑151 (MQ=255)
                                                                                       cGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAgtcgggtcg                                                            <  2:4339135/151‑1 (MQ=255)
                                                                                           gATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGc                                                        >  1:2081635/1‑151 (MQ=255)
                                                                                           gATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGc                                                        >  1:26367/1‑151 (MQ=255)
                                                                                            aTGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCg                                                       <  1:1216307/151‑1 (MQ=255)
                                                                                             tGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCgg                                                      <  1:2961747/151‑1 (MQ=255)
                                                                                               gCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGtc                                                    <  2:1430806/151‑1 (MQ=255)
                                                                                                 tgtgTCGGCATCGCCAACCTCGGCCAGCCCGGGCAACGGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGtctc                                                  <  1:237051/151‑1 (MQ=255)
                                                                                                 tgggtCGGCATCGGCACCCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCGCTCGCTAGTCGGGTCGGGGCGGtctc                                                  <  2:4709969/148‑1 (MQ=255)
                                                                                                      cGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGa                                             <  1:182159/151‑1 (MQ=255)
                                                                                                      cGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGa                                             <  2:746675/151‑1 (MQ=255)
                                                                                                         cATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGAAcc                                          >  1:649370/1‑151 (MQ=255)
                                                                                                         cATCGACAACCTCGACCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGAAcc                                          <  2:2308884/151‑1 (MQ=255)
                                                                                                          aTCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGAACcg                                         <  1:1710656/151‑1 (MQ=255)
                                                                                                          aTCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGAACcg                                         >  2:3937077/1‑151 (MQ=255)
                                                                                                           tCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGAACcgc                                        <  2:2141993/151‑1 (MQ=255)
                                                                                                            cGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGAACcgcg                                       <  1:1509593/151‑1 (MQ=255)
                                                                                                            cGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGAACcgcg                                       >  1:3762293/1‑151 (MQ=255)
                                                                                                                aaCCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGAACCGCGacac                                   >  1:5230084/1‑151 (MQ=255)
                                                                                                                 aCCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGAACCGCGACACt                                  >  1:2421067/1‑151 (MQ=255)
                                                                                                                 aCCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGAACCGCGACACt                                  >  1:5167084/1‑151 (MQ=255)
                                                                                                                  ccTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGGTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGGCCGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGAACCGCGACACTg                                 <  2:547165/151‑1 (MQ=255)
                                                                                                                   cTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGAACCGCGACACTgg                                >  1:227795/1‑151 (MQ=255)
                                                                                                                     cGGCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGAACCGCGACACTgggg                              >  1:4084259/1‑151 (MQ=255)
                                                                                                                      ggCCAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGCCTTCATTCCGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGAACCGCGACACTGGGGt                             <  2:4616602/151‑1 (MQ=255)
                                                                                                                        ccAGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGAACCGCGACACTGGGGTTa                           <  1:608459/151‑1 (MQ=255)
                                                                                                                          aGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGAACCGCGACACTGGGGTTACt                         >  1:1446575/1‑151 (MQ=255)
                                                                                                                          aGCCCGGGCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGAACCGCGACACTGGGGTTACt                         >  1:4590781/1‑151 (MQ=255)
                                                                                                                                ggCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGAACCGCGACACTGGGGTTACTGGCAgt                   <  1:2678886/151‑1 (MQ=255)
                                                                                                                                ggCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGAACCGCGACACTGGGGTTACTGGCAgt                   >  2:783327/1‑151 (MQ=255)
                                                                                                                                ggCAACTGCGTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCAAGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGAACCGCGACACTGGGGTTACTGGCAgt                   <  1:3067528/151‑1 (MQ=255)
                                                                                                                                         gTCCGCTGCTCAGCCACGTTGAAGACTTCATTCAGTCCGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGAACCGCGACACTGGGGTTACTGGCAGTGTAACCGaa          <  2:3824242/151‑1 (MQ=255)
                                                                                                                                         gTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGAACCGCGACACTGGGGTTACTGGCGGTGTAACCGaa          >  1:4734784/1‑151 (MQ=255)
                                                                                                                                         gTCCACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGGCGGGGCGGTCTCGGGGAACCGCGACACTGGGGTTACTGGCAGTGTAACCGaa          >  1:3686949/1‑151 (MQ=255)
                                                                                                                                            cACTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGAACCGCGACACTGGGGTTACTGGCAGTGTAACCGaacaa       >  2:4288025/1‑151 (MQ=255)
                                                                                                                                             aCTGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGAACCGCGACACTGGGGTTACTGGCAGTGTAACCGaacaac      >  1:2779646/1‑151 (MQ=255)
                                                                                                                                               tGCTCAGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGAACCGCGACACTGGGGTTACTGGCAGTGTAACCGAACAACtt    >  1:3341975/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGTTGTCCCAGTGGCAGCCCTCTTCGTCGGCGGCGCGGCTGAGCGGCGCAATGGTCCTGGCGGTTACCTTGCCGGCTTCTTCCAGCACGGCGATGGCTGTGTCGGCATCGACAACCTCGGCCAGCCCGGGCAACTGCGTCCACTGCTCGGCCACGTTGAAGACTTCATTCAGTACGAAGCGCATGTCGCGCAGGGGCGCTTTATAGTCAGCCATGACAACCTCTCGCTAGTCGGGTCGGGGCGGTCTCGGGGAACCGCGACACTGGGGTTACTGGCAGTGTAACCGAACAACTTTT  >  CP009974/1654795‑1655090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑