Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 380,918 C→T T135I (ACC→ATC)  RPPX_01535 → guanine permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009974380,9180CT92.4% 725.4 / 30.4 226T135I (ACC→ATC) RPPX_01535guanine permease
Reads supporting (aligned to +/- strand):  ref base C (6/11);  new base T (109/99);  total (115/111)
Fisher's exact test for biased strand distribution p-value = 2.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.26e-01

Only 100 of 226 total aligned reads displayed.

CCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATGGGGCTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGACCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGCAGGCATCGTCGTCGACAGCCCCGCCACCCTGATCAAGCT  >  CP009974/380772‑381065
                                                                                                                                                  |                                                                                                                                                   
ccTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATGGGGCTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTg                                                                                                                                                 <  1:4440440/151‑1 (MQ=255)
  tGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATGGGGCTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTc                                                                                                                                               <  1:2263704/151‑1 (MQ=255)
   gTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATGGGGCTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCg                                                                                                                                              <  2:627645/151‑1 (MQ=255)
           aCTGGCCGGTGGGCCTGGCGCCGGGCATGGGGCTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTgcg                                                                                                                                      <  1:1393634/151‑1 (MQ=255)
           aCTGGCCGGTGGGCCTGGCGCCGGGCATGGGGCTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTgcg                                                                                                                                      <  2:454250/151‑1 (MQ=255)
            cTGGCCGGTGGGCCTGGCGCCGGGCATGGGGCTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTgcgc                                                                                                                                     <  2:2953299/151‑1 (MQ=255)
             tGGCCGGTGGGCCTGGCGCCGGGCATGGGGCTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGcgcg                                                                                                                                    <  1:4280288/151‑1 (MQ=255)
                 cGGTGGGCCTGGCGCCGGGCATGGGGCTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATg                                                                                                                                >  1:2700503/1‑151 (MQ=255)
                  ggTGGGCCTGGCGCCGGGCATGGGGCTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATgg                                                                                                                               >  1:4076849/1‑151 (MQ=255)
                  ggTGGGCCTGGCGCCGGGCATGGGGCTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATgg                                                                                                                               >  1:2527388/1‑151 (MQ=255)
                   gTGGGCCTGGCGCCGGGCATGGGGCTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGACCTTGTCGAAAGTGCGCGAATGgc                                                                                                                              <  1:5230743/151‑1 (MQ=255)
                    tGGGCCTGGCGCCGGGCATGGGGCTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGgct                                                                                                                             >  1:3968604/1‑151 (MQ=255)
                    tGGGCCTGGCGCCGGGCATGGGGCTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGgct                                                                                                                             <  2:4833769/151‑1 (MQ=255)
                      ggCCTGGCGCCGGGCATGGGGCTCAATGCCTTCTTTACCTGCACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGgctgc                                                                                                                           <  1:395740/151‑1 (MQ=255)
                           ggCGCCGGGCATGGGGCTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAAc                                                                                                                      <  1:1900590/151‑1 (MQ=255)
                           ggCGCCGGGCATGGGGCTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAAc                                                                                                                      <  1:1473155/151‑1 (MQ=255)
                             cgcCGGGCATGGGGCTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAg                                                                                                                    >  1:1992948/1‑151 (MQ=255)
                             cgcCGGGCATGGGGCTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAg                                                                                                                    <  2:1230764/151‑1 (MQ=255)
                               ccGGGCATGGGGCTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTTGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGTTCAACATCa                                                                                                                  >  2:2748787/1‑151 (MQ=255)
                                cGGGCATGGGGCTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCAt                                                                                                                 <  2:2310675/151‑1 (MQ=255)
                                cGGGCATGGGGCTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCAt                                                                                                                 >  1:609996/1‑151 (MQ=255)
                                 gggCATGGGGCTCAATGCCTTCTCTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGACCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATc                                                                                                                <  1:955755/151‑1 (MQ=255)
                                  ggCATGGGGCTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATcc                                                                                                               <  1:4191667/151‑1 (MQ=255)
                                    cATGGGGCTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCg                                                                                                             <  1:2794589/151‑1 (MQ=255)
                                      tGGGGCTCAATGCCTTCTTTACCTACAGCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGt                                                                                                           <  1:4343093/151‑1 (MQ=255)
                                        gggCTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCa                                                                                                         >  2:513048/1‑151 (MQ=255)
                                        gggCTCAATGCCTTCTGTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAGCGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCa                                                                                                         <  2:3609706/151‑1 (MQ=255)
                                         ggCTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAg                                                                                                        >  2:1244602/1‑151 (MQ=255)
                                         ggCTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGACCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAg                                                                                                        <  1:2640661/151‑1 (MQ=255)
                                           cTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGcc                                                                                                      <  1:1969748/151‑1 (MQ=255)
                                           cTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGACCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGcc                                                                                                      >  2:3368804/1‑151 (MQ=255)
                                             cAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTg                                                                                                    >  2:4229778/1‑151 (MQ=255)
                                              aaTGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTgc                                                                                                   >  1:3573155/1‑151 (MQ=255)
                                              aaTGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTgc                                                                                                   <  1:2750463/151‑1 (MQ=255)
                                              aaTGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTgc                                                                                                   >  2:2124458/1‑151 (MQ=255)
                                               aTGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTgcg                                                                                                  >  2:1976927/1‑151 (MQ=255)
                                                 gCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCgcc                                                                                                >  2:1106623/1‑151 (MQ=255)
                                                   cttcttTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAAc                                                                                                                      <  1:5004238/127‑1 (MQ=255)
                                                   cttcttTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAAc                                                                                                                      >  2:5004238/1‑127 (MQ=255)
                                                   cttcttTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTTAGCCTGCgccat                                                                                              >  2:2062368/1‑151 (MQ=255)
                                                   cttcttTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCgccat                                                                                              <  1:3484364/151‑1 (MQ=255)
                                                   cttcttTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCgccat                                                                                              >  2:391031/1‑151 (MQ=255)
                                                     tcttGACCTACGCCGTGGTCGGCACCATGGGCTAAAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCgccatgc                                                                                            <  2:1846568/151‑1 (MQ=255)
                                                      cttTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCgccatgcc                                                                                           >  2:1347026/1‑151 (MQ=255)
                                                       tttACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCgccatgcca                                                                                          >  1:4068750/1‑151 (MQ=255)
                                                        ttACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCgccatgccat                                                                                         <  2:1914140/151‑1 (MQ=255)
                                                          aCCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATgg                                                                                       <  1:1234841/151‑1 (MQ=255)
                                                           ccTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATggg                                                                                      >  1:812692/1‑151 (MQ=255)
                                                               cacCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGcc                                                                                  <  1:3716802/151‑1 (MQ=255)
                                                               cacCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGcc                                                                                  >  2:1022791/1‑151 (MQ=255)
                                                               cacCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGACCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGcc                                                                                  <  2:2885499/151‑1 (MQ=255)
                                                                cccGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCg                                                                                 <  2:4135467/150‑1 (MQ=255)
                                                                acCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCg                                                                                 >  2:2434523/1‑151 (MQ=255)
                                                                       ttGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCgg                                                                          >  2:1526219/1‑151 (MQ=255)
                                                                       ttGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCgg                                                                          >  1:1018433/1‑151 (MQ=255)
                                                                        gggCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGACCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGa                                                                         <  2:4451445/150‑1 (MQ=255)
                                                                           cACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGGCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTg                                                                      <  2:1217294/151‑1 (MQ=255)
                                                                            aCCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGACCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGt                                                                     >  2:4319849/1‑151 (MQ=255)
                                                                              cATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGttt                                                                   >  1:984134/1‑151 (MQ=255)
                                                                               aTGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTtc                                                                  >  1:4969166/1‑151 (MQ=255)
                                                                                tGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTtct                                                                 >  1:1190311/1‑151 (MQ=255)
                                                                                tGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTtct                                                                 <  1:1699813/151‑1 (MQ=255)
                                                                                 gggCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTtctc                                                                >  1:3021638/1‑151 (MQ=255)
                                                                                     tACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGACCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGc                                                            >  2:606286/1‑151 (MQ=255)
                                                                                           tGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCg                                                      >  2:74727/1‑151 (MQ=255)
                                                                                              gAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGcn                                                   <  1:3670850/151‑2 (MQ=255)
                                                                                              gAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGcc                                                   >  2:406053/1‑151 (MQ=255)
                                                                                                aaCGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTg                                                 <  1:3703928/151‑1 (MQ=255)
                                                                                                   ggCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAg                                              <  2:5171308/151‑1 (MQ=255)
                                                                                                     cgcTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGAc                                            >  2:3630388/1‑151 (MQ=255)
                                                                                                        tGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGc                                         >  2:3699703/1‑151 (MQ=255)
                                                                                                         gggggCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGCa                                        >  1:2832415/1‑151 (MQ=255)
                                                                                                          ggggCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGACCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGCAg                                       <  2:3501513/151‑1 (MQ=255)
                                                                                                            ggCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGCAGGc                                     >  2:5075036/1‑151 (MQ=255)
                                                                                                             gCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGGTTCTCGGGCTGATCGGCCTGACGACTGCAGGCa                                    >  2:341698/1‑151 (MQ=255)
                                                                                                              cGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGCAGGCAt                                   >  1:3709327/1‑151 (MQ=255)
                                                                                                                gTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGCAGGCAtcg                                 >  2:3578535/1‑151 (MQ=255)
                                                                                                                 tATTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGCAGGCAtcgt                                >  1:1832114/1‑151 (MQ=255)
                                                                                                                  aTTCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGCAGGCAtcgtc                               <  2:3202052/151‑1 (MQ=255)
                                                                                                                    tcgtcgCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGCAGGCAtcgtcgt                             <  2:3297837/151‑1 (MQ=255)
                                                                                                                    tCGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGCAGGCAtcgtcgt                             <  2:459888/151‑1 (MQ=255)
                                                                                                                     cGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGCAGGCAtcgtcgtc                            <  2:952030/151‑1 (MQ=255)
                                                                                                                     cGTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCCGCAGCCTGCAGGCAtcgtcgtc                            >  2:3985598/1‑151 (MQ=255)
                                                                                                                      gTCTCGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGCAGGCAtcgtcgtcg                           >  1:2065081/1‑151 (MQ=255)
                                                                                                                       tctcGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGCAGGCATCGTCGTCGa                          >  2:1523030/1‑151 (MQ=255)
                                                                                                                         tcGGGCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGCAGGCATCGTCGTCGACa                        >  1:4495051/1‑151 (MQ=255)
                                                                                                                           gggCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGCAGGCATCGTCGTCGACAGc                      <  2:2190769/151‑1 (MQ=255)
                                                                                                                             gCGTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGCAGGCATCGTCGTCGACAGccc                    <  2:2182418/151‑1 (MQ=255)
                                                                                                                             gCGTGCTGTTCATGTTCCTGACCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGCAGGCATCGTCGTCGACAGccc                    <  1:460188/151‑1 (MQ=255)
                                                                                                                               gTGCTGTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGCAGGCATCGTCGTCGACAGCCCCg                  <  2:799318/151‑1 (MQ=255)
                                                                                                                                    gTTCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGCAGGCATCGTCGTCGACAGCCCTGCcccc             >  2:98721/1‑151 (MQ=255)
                                                                                                                                     ttCATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGCAGGCATCGTCGTCGACAGCCCCGCCAccc            >  1:2953645/1‑151 (MQ=255)
                                                                                                                                       cATGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGCAGGCATCGTCGTCGACAGCCCCGCCACCCTg          >  2:962165/1‑151 (MQ=255)
                                                                                                                                        aTGTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGCAGGCATCGTCGTCGACAGCCCCGCCACCCTGa         >  1:2581142/1‑151 (MQ=255)
                                                                                                                                          gTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGCAGGCATCGTCGTCGACAGCCCCGCCACCCTGATc       >  1:4240625/1‑151 (MQ=255)
                                                                                                                                          gTTCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGCAGGCATCGTCGTCGACAGCCCCGCCACCCTGATc       >  1:1417154/1‑151 (MQ=255)
                                                                                                                                           ttCCTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGCCCGGCGCCGGATTGTTTCTCGGGCTGCTCGGCCTGAAGACTGCAGGCAACGGCGTCGACCGCCCCGCCAACCtcatca      >  2:4760161/1‑151 (MQ=255)
                                                                                                                                             ccTGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGCAGGCATCGTCGTCGACAGCCCCGCCACCCTGATCAAg    >  1:3618745/1‑151 (MQ=255)
                                                                                                                                               tGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGCAGGCATCGTCGTCGACAGCCCCGCCACCCTGATCAAGCt  >  1:3226175/1‑151 (MQ=255)
                                                                                                                                               tGATCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGCAGGCATCGTCGTCGACAGCCCCGCCACCCTGATCAAGCt  >  1:1087652/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATGGGGCTCAATGCCTTCTTTACCTACACCGTGGTTGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGCTGTTCATGTTCCTGACCTTGTCGAAAGTGCGCGAATGGCTGCTCAACAGCATCCCGGTCAGCCTGCGCCATGCCATGGGGGCCGGCGTCGGATTGTTTCTCGGGCTGATCGGCCTGAAGACTGCAGGCATCGTCGTCGACAGCCCCGCCACCCTGATCAAGCT  >  CP009974/380772‑381065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑