Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 1,657,996 G→A A200A (GCC→GCT)  RPPX_07420 ← acyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099741,657,9960GA99.2% 1005.8 / ‑5.3 268A200A (GCC→GCT) RPPX_07420acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (138/128);  total (139/129)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 268 total aligned reads displayed.

TCACGCCGTTGCGCTCGCCCACACCGCCTTCGGCGTTGGGCAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCGGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGCACATGGTGCCGGTCCACTCGCCGGACACCAGCTTGGTC  >  CP009974/1657848‑1658143
                                                                                                                                                    |                                                                                                                                                   
tCACGCCGTTGCGCTCGCCCACACCGCCTTCGGCGTTGGGCAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGc                                                                                                                                                   <  1:2793624/151‑1 (MQ=255)
 cACGCCGTTGCGCTCGCCCACACCGCCTTCGGCGTTGGGCAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGcc                                                                                                                                                  >  1:712542/1‑151 (MQ=255)
    gCCGTTGCGCTCGCCCACACCGCCTTCGGCGTTGGGCAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGaa                                                                                                                                               <  1:2614655/151‑1 (MQ=255)
      cGTTGCGCTCGCCCACACCGCCTTCGGCGTTGGGCAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAAt                                                                                                                                             <  1:3050602/151‑1 (MQ=255)
       gTTGCGCTCGCCCACACCGCCTTCGGCGTTGGGCAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATg                                                                                                                                            >  1:1870387/1‑151 (MQ=255)
        ttGCGCTCGCCCACACCGCCTTCGGCGTTGGGCAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGa                                                                                                                                           >  1:4645280/1‑151 (MQ=255)
           cgcTCGCCCACACCGCCTTCGGCGTTGGGCAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGa                                                                                                                                        >  2:1246285/1‑151 (MQ=255)
            gcTCGCCCACACCGCCTTCGGCGTTGGGCAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGAt                                                                                                                                       >  1:248511/1‑151 (MQ=255)
             cTCGCCCACACCGCCTTCGGCGTTGGGCAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATc                                                                                                                                      <  2:2259572/151‑1 (MQ=255)
               ggcacacgCCGCCTTCGGGGTTGGGGAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCtt                                                                                                                                    <  2:2991029/147‑1 (MQ=255)
                  ccACACCGCCTTCGGCGTTGGGCAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGt                                                                                                                                 >  2:3126049/1‑151 (MQ=255)
                   cacaCCGCCTTCGGCGTTGGGCAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTg                                                                                                                                <  1:3743806/151‑1 (MQ=255)
                    acacCGCCTTCGGCGTTGGGCAGGAACTGCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGc                                                                                                                               <  2:4485434/151‑1 (MQ=255)
                        cGCCTTCGGCGTTGGGCAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGa                                                                                                                           <  1:4752158/151‑1 (MQ=255)
                        cGCCTTCGGCGTTGGGCAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGa                                                                                                                           >  2:1626094/1‑151 (MQ=255)
                         gCCTTCGGCGTTGGGCAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGac                                                                                                                          <  2:2143832/151‑1 (MQ=255)
                         gCCTTCGGCGTTGGGCAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGac                                                                                                                          >  2:4324599/1‑151 (MQ=255)
                           cTTCGGCGTTGGGCAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGacac                                                                                                                        >  2:1499051/1‑151 (MQ=255)
                            ttCGGCGTTGGGCAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACt                                                                                                                       <  1:2130936/151‑1 (MQ=255)
                               ggCGTTGGGCAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTg                                                                                                                    <  1:383487/151‑1 (MQ=255)
                                gCGTTGGGCAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGt                                                                                                                   >  2:3615361/1‑151 (MQ=255)
                                  ttttggCAGGAACTTCGGCACGATGAGCAGGGAATTACCCTTGGGGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAg                                                                                                                 <  2:1759728/147‑1 (MQ=255)
                                  gTTGGGCAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAg                                                                                                                 >  2:1136162/1‑151 (MQ=255)
                                   ttGGGCAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGc                                                                                                                >  1:530856/1‑151 (MQ=255)
                                    tGGGCAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCt                                                                                                               <  2:1349784/151‑1 (MQ=255)
                                       gCAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTAcc                                                                                                            >  1:3092344/1‑151 (MQ=255)
                                       gCAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTAcc                                                                                                            <  1:4088380/151‑1 (MQ=255)
                                        cAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCa                                                                                                           <  2:1425664/151‑1 (MQ=255)
                                        cAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCa                                                                                                           >  1:88006/1‑151 (MQ=255)
                                         aGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCAt                                                                                                          >  1:3476747/1‑151 (MQ=255)
                                         aGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCAt                                                                                                          <  1:1546913/151‑1 (MQ=255)
                                             aCTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTATCTACCATCGGc                                                                                                      >  2:329589/1‑151 (MQ=255)
                                              cTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGcc                                                                                                     <  1:4019768/151‑1 (MQ=255)
                                               ttCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCt                                                                                                    <  1:4303844/151‑1 (MQ=255)
                                               ttCGGCACGATAAATACTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCAAGAACGATATGGACGATGTTGTCGGTCATGTCGTGCTCACCAGCCGACATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCCTCGGCCt                                                                                                    <  2:4185567/151‑1 (MQ=255)
                                               gtcggggcGATGAACAGTGAAGTACGCTTGGTGCCGACCGGTGCGTCCGGCAGGCGGGCGAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCt                                                                                                    <  2:4596056/144‑1 (MQ=255)
                                                tCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCtg                                                                                                   >  1:2233475/1‑151 (MQ=255)
                                                 cGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCtgt                                                                                                  >  1:610754/1‑151 (MQ=255)
                                                  ggCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCtgtg                                                                                                 <  2:1705246/151‑1 (MQ=255)
                                                   gaaCGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTgg                                                                                                <  2:169793/149‑1 (MQ=255)
                                                   gCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTgg                                                                                                >  2:712184/1‑151 (MQ=255)
                                                    cACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGc                                                                                               >  2:3170242/1‑151 (MQ=255)
                                                      cGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTc                                                                                             <  1:837131/151‑1 (MQ=255)
                                                              aGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGCTCTTGGTGCCAGACACCTTGTAGATACCATCGGCCTGTGGCTCGGCCTTgg                                                                                     >  2:3999493/1‑151 (MQ=255)
                                                              aGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTgg                                                                                     >  1:4881797/1‑151 (MQ=255)
                                                                tGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTg                                                                                   >  2:949369/1‑151 (MQ=255)
                                                                    aTACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCgca                                                                               <  1:2483968/151‑1 (MQ=255)
                                                                          ttGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGc                                                                         >  1:1789412/1‑151 (MQ=255)
                                                                          ttGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGc                                                                         <  1:241861/151‑1 (MQ=255)
                                                                           tGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGACATGAAGATCTTGGTGCCCGACACTTTGTAGCTATCATCGGACTGTGGCTCGGCATTGTTGCGCAGCATGcc                                                                        >  2:2743847/1‑151 (MQ=255)
                                                                             gTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCa                                                                      <  2:392658/151‑1 (MQ=255)
                                                                               gccggccgGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAgg                                                                    <  1:774140/151‑1 (MQ=255)
                                                                                ccggccggTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAggt                                                                   >  1:3582828/1‑151 (MQ=255)
                                                                                 cggccggTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAACCGAAATGAAGCTCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTTCGCAGCATGCCCAggtc                                                                  >  2:1822552/1‑151 (MQ=255)
                                                                                  ggccggTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAggtcg                                                                 >  1:4452937/1‑151 (MQ=255)
                                                                                    ccggTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAggtcggt                                                               >  1:1587551/1‑151 (MQ=255)
                                                                                    ccggTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAggtcggt                                                               <  2:237955/151‑1 (MQ=255)
                                                                                      ggTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTcc                                                             >  2:728837/1‑151 (MQ=255)
                                                                                      ggTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTcc                                                             <  2:2321286/151‑1 (MQ=255)
                                                                                      ggTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCACGCCCAGGTCGGccc                                                             >  2:2125916/1‑151 (MQ=255)
                                                                                       gTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGAAGCATGCCCAGGTCGGTccc                                                            <  1:23921/151‑1 (MQ=255)
                                                                                        tGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGTTTCGCAGCATGCCCACGTCGGTCCCg                                                           >  1:2187738/1‑151 (MQ=255)
                                                                                           gTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAg                                                        >  1:238684/1‑151 (MQ=255)
                                                                                             ccGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTg                                                      >  2:4623086/1‑151 (MQ=255)
                                                                                               ggCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCg                                                    >  2:2006165/1‑151 (MQ=255)
                                                                                                 cAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCCTGCCCAGGTCGGTCCCGCAGTGCggg                                                  >  2:4063447/1‑150 (MQ=255)
                                                                                                  aGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGtt                                                 >  1:3994392/1‑151 (MQ=255)
                                                                                                   ggCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTtc                                                >  2:373023/1‑151 (MQ=255)
                                                                                                     cGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCCGTGCGGTtcag                                              >  1:905825/1‑151 (MQ=255)
                                                                                                      gggCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTtcagt                                             >  1:4266905/1‑151 (MQ=255)
                                                                                                         ccAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTtcagtcag                                          <  1:3344654/151‑1 (MQ=255)
                                                                                                          cAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAgg                                         <  1:1265409/151‑1 (MQ=255)
                                                                                                           aGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGc                                        >  1:3434659/1‑151 (MQ=255)
                                                                                                               cGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGCACAt                                    <  1:4128598/151‑1 (MQ=255)
                                                                                                                  tatGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGCACATGGt                                 <  2:535619/151‑1 (MQ=255)
                                                                                                                    tGGACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGCACATGGTGc                               <  2:4726681/151‑1 (MQ=255)
                                                                                                                     ggACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGCACATGGTGcc                              <  1:3170242/151‑1 (MQ=255)
                                                                                                                      gACGATGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGCACATGGTGCCg                             <  1:3705096/151‑1 (MQ=255)
                                                                                                                          aTGTTGTCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGCACATGGTGCCGGTcc                         >  1:5175678/1‑151 (MQ=255)
                                                                                                                            gttgtCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGCACATGGTGCCGGTCCAc                       <  2:504242/151‑1 (MQ=255)
                                                                                                                             ttgtCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGCACATGGTGCCGGTCCACt                      <  2:3241095/151‑1 (MQ=255)
                                                                                                                              tgtCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGCACATGGTGCCGGTCCACTc                     <  2:1088791/151‑1 (MQ=255)
                                                                                                                              tgtCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGCACATGGTGCCGGTCCACTc                     <  1:2006505/151‑1 (MQ=255)
                                                                                                                               gtCGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGCACATGGTGCCGGTCCACTCg                    <  2:758766/151‑1 (MQ=255)
                                                                                                                                 cGGCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGCACATGGTGCCGGTCCACTCGcc                  <  2:3224592/151‑1 (MQ=255)
                                                                                                                                  ggCCATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGCACATGGTGCCGGTCCACTCGCCg                 <  1:2937995/151‑1 (MQ=255)
                                                                                                                                    ccATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGCACATGGTGCCGGTCCACTCGCCGGa               <  1:1075246/151‑1 (MQ=255)
                                                                                                                                     cATGTCGTGCTCACCGGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGCACATGGTGCCGGTCCACTCGCCGGac              >  1:1895312/1‑151 (MQ=255)
                                                                                                                                     cATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGCACATGGTGCCGGTCCACTCGCCGGac              >  1:4325186/1‑151 (MQ=255)
                                                                                                                                     cATGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGGGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGCACATGGTGCCGGTCCACTCGCCGGag              >  2:4346933/1‑150 (MQ=255)
                                                                                                                                      gtGTCGTGCTCACCAGCCGAAATGAAGATCGTGGTGGCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGCACATGGTGCCGGTCCACTCGCCGGaca             <  2:2671742/150‑1 (MQ=255)
                                                                                                                                       tGTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGCACATGGTGCCGGTCCACTCGCCGGacac            <  1:3928901/151‑1 (MQ=255)
                                                                                                                                        gTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGCACATGGTGCCGGTCCACTCGCCGGACAcc           <  1:1338420/151‑1 (MQ=255)
                                                                                                                                        gTCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGCACATGGTGCCGGTCCACTCGCCGGACAcc           >  2:2538948/1‑151 (MQ=255)
                                                                                                                                         tCGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGCACATGGTGCCGGTCCACTCGCCGGACACCa          <  2:16478/151‑1 (MQ=255)
                                                                                                                                          cGTGCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGCACATGGTGCCGGTCCACTCGCCGGACACCAg         >  1:2562355/1‑151 (MQ=255)
                                                                                                                                             gCTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGCACATGGTGCCGGTCCACTCGCCGGACACCAGCtt      >  1:984783/1‑151 (MQ=255)
                                                                                                                                              cTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGCACATGGTGCCGGTCCACTCGCCGGACACCAGCTTg     >  2:3855670/1‑151 (MQ=255)
                                                                                                                                              cTCACCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGATCCCGCAGTGCGGTTCAGTCAGGCACATGGTGCCGGTCCACTCGCCGGACACCAGCtcg     >  2:4975609/1‑149 (MQ=255)
                                                                                                                                                 aCCAGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGCACATGGTGCCGGTCCACTCGCCGGACACCAGCTTGGTc  <  1:3913302/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCACGCCGTTGCGCTCGCCCACACCGCCTTCGGCGTTGGGCAGGAACTTCGGCACGATGAACAGTGAAATACCCTTGGTGCCGGCCGGTGCGTCCGGCAGGCGGGCCAGAACGATATGGACGATGTTGTCGGCCATGTCGTGCTCACCGGCCGAAATGAAGATCTTGGTGCCCGACACTTTGTAGCTACCATCGGCCTGTGGCTCGGCCTTGGTGCGCAGCATGCCCAGGTCGGTCCCGCAGTGCGGTTCAGTCAGGCACATGGTGCCGGTCCACTCGCCGGACACCAGCTTGGTC  >  CP009974/1657848‑1658143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑