Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 1,662,344 G→A L160L (CTG→TTG)  RPPX_07445 ← transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099741,662,3440GA94.0% 959.1 / 24.3 284L160L (CTG→TTG) RPPX_07445transporter
Reads supporting (aligned to +/- strand):  ref base G (8/9);  new base A (119/148);  total (127/157)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 284 total aligned reads displayed.

CATCGGGCAACAGCTCGCTCAACGCCTTAGCCGCCTCTGCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAGGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATGGTTGCGGCAACCGTCGAGGAAGGTGCCGAAGGT  >  CP009974/1662197‑1662491
                                                                                                                                                   |                                                                                                                                                   
cATCGGGCAACAGCTCGCTCAACGCCTTAGCCGCCTCTGCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTa                                                                                                                                                  <  2:2516536/151‑1 (MQ=255)
cATCGGGCAACAGCTCGCTCAACGCCTTAGCCGCCTCTGCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTa                                                                                                                                                  <  2:1036945/151‑1 (MQ=255)
cATCGGGCAACAACTCGCTCAACGCCTTAGCCGCCTCTGCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTa                                                                                                                                                  <  1:2480959/151‑1 (MQ=255)
  tCGGGCAACAGCTCGCTCAACGCCTTAGCCGCCTCTGCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACa                                                                                                                                                >  1:3285403/1‑151 (MQ=255)
    gggCAACAGCTCGCTCAACGCCTTAGCCGCCTCTGCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAAc                                                                                                                                              <  1:970368/151‑1 (MQ=255)
     ggCAACAGCTCGCTCAACGCCTTAGCCGCCTCTGCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCCTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACg                                                                                                                                             >  2:1549462/1‑151 (MQ=255)
      gCAACAGCTCGCTCAACGCCTTAGCCGCCTCTGCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACgg                                                                                                                                            <  1:1498612/151‑1 (MQ=255)
          cAGCTCGCTCAACGCCTTAGCCGCCTCTGCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCg                                                                                                                                        <  2:1734214/151‑1 (MQ=255)
           aGCTCGCTCAACGCCTTAGCCGCCTCTGCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCgg                                                                                                                                       >  2:4261093/1‑151 (MQ=255)
               cgctcAACGCCTTAGCCGCCTCTGCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATc                                                                                                                                   >  2:146793/1‑151 (MQ=255)
                gctcAACGCCTTAGCCGCCTCTGCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATcc                                                                                                                                  <  2:2706456/151‑1 (MQ=255)
                     aCGCCTTAGCCGCCTCTGCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCAc                                                                                                                             <  2:700110/151‑1 (MQ=255)
                      cGCCTTAGCCGCCTCTGCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACg                                                                                                                            <  1:921539/151‑1 (MQ=255)
                       gCCTTAGCCGCCTCTGCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGc                                                                                                                           <  2:2296114/151‑1 (MQ=255)
                         cTTAGCCGCCTCTGCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCg                                                                                                                         <  2:4706747/151‑1 (MQ=255)
                          ttAGCCGCCTCTGCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCCGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGa                                                                                                                        >  2:3485960/1‑151 (MQ=255)
                          ttAGCCGCCTCTGCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAGGTACAACGGGTCGGTATCCGGCACGCCGa                                                                                                                        <  2:2381821/151‑1 (MQ=255)
                             gccgccTCTGCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGacac                                                                                                                     >  2:2568082/1‑151 (MQ=255)
                              ccgccTCTGCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACAcc                                                                                                                    >  1:4616112/1‑151 (MQ=255)
                               cgccTCTGCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACAccc                                                                                                                   >  1:3379813/1‑151 (MQ=255)
                                   tctGCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGtt                                                                                                               >  2:1578013/1‑151 (MQ=255)
                                   tctGCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGtt                                                                                                               >  1:1789405/1‑151 (MQ=255)
                                    ctGCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTg                                                                                                              <  1:5070507/151‑1 (MQ=255)
                                      gCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCa                                                                                                                              <  2:561626/133‑1 (MQ=255)
                                      gCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCa                                                                                                                              >  1:561626/1‑133 (MQ=255)
                                      gCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGgc                                                                                                            >  2:893520/1‑151 (MQ=255)
                                      gCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGACGACACCCAGTTGgc                                                                                                            <  2:621085/151‑1 (MQ=255)
                                        cGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGgcgc                                                                                                          >  1:1203473/1‑151 (MQ=255)
                                            aCTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACa                                                                                                      <  1:3586259/151‑1 (MQ=255)
                                              tAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAgg                                                                                                    <  1:5125091/151‑1 (MQ=255)
                                                gTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGt                                                                                                  <  1:2223269/151‑1 (MQ=255)
                                                 tGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTg                                                                                                 <  2:1181988/151‑1 (MQ=255)
                                                      cGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCgg                                                                                            <  1:4544037/151‑1 (MQ=255)
                                                       gCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGc                                                                                           <  2:956045/151‑1 (MQ=255)
                                                        cTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCt                                                                                          >  1:1929532/1‑151 (MQ=255)
                                                         tGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTg                                                                                         >  2:904214/1‑151 (MQ=255)
                                                          gCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGc                                                                                        <  1:299162/151‑1 (MQ=255)
                                                          gCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGc                                                                                        >  1:4474155/1‑151 (MQ=255)
                                                           ccGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCa                                                                                       <  2:4514226/151‑1 (MQ=255)
                                                           ccGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCa                                                                                       <  1:2814869/151‑1 (MQ=255)
                                                            cGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAg                                                                                      >  1:3456457/1‑151 (MQ=255)
                                                               cGAGCAGGTGCAGTTGCGGGCCCTCATAGGACTGCCAGGCTTCACCGGCGTCCAGCTTGGCCAGCACGTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGgc                                                                                   <  2:3130375/151‑1 (MQ=255)
                                                                     ggTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTc                                                                             <  1:4169122/151‑1 (MQ=255)
                                                                      gTGCAGTTGCGGGCCGTCATGGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCg                                                                            <  2:2279891/151‑1 (MQ=255)
                                                                       tGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGc                                                                           >  1:907553/1‑151 (MQ=255)
                                                                        gCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCt                                                                          >  1:791855/1‑151 (MQ=255)
                                                                             tGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGcaca                                                                     <  2:4991751/151‑1 (MQ=255)
                                                                               cGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAgg                                                                   <  1:2508047/151‑1 (MQ=255)
                                                                                gggCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAggg                                                                  >  1:711986/1‑151 (MQ=255)
                                                                                  gCCATCATAGGCCTGCAAGGCTTCACGGTTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGTCCAGGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTa                                                                <  2:185199/151‑1 (MQ=255)
                                                                                   ccAGCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGCCCGGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTAc                                                               <  1:1050682/151‑1 (MQ=255)
                                                                                    catcatAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACg                                                              >  1:3406660/1‑151 (MQ=255)
                                                                                     atcatAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACgg                                                             >  2:4869528/1‑151 (MQ=255)
                                                                                     atcatAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACgg                                                             <  2:4350259/151‑1 (MQ=255)
                                                                                       catAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAGGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGaa                                                           >  1:5199747/1‑151 (MQ=255)
                                                                                          aGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAgcg                                                        <  2:1091859/151‑1 (MQ=255)
                                                                                          aGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAgcg                                                        >  1:3834927/1‑151 (MQ=255)
                                                                                            gCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCt                                                      >  1:331726/1‑151 (MQ=255)
                                                                                            gCCTGCAAGGCTTCACGGGGGTCCAGCTTGGCCAGCAGTTCAAGGCCAGTGGGCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCt                                                      <  2:2801352/151‑1 (MQ=255)
                                                                                             ccTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCtt                                                     <  2:347572/151‑1 (MQ=255)
                                                                                              cTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTg                                                    <  2:4384959/151‑1 (MQ=255)
                                                                                              cTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTg                                                    <  2:3528959/151‑1 (MQ=255)
                                                                                                 cAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAgg                                                 <  1:1373682/151‑1 (MQ=255)
                                                                                                  aaGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAggg                                                >  2:3606749/1‑151 (MQ=255)
                                                                                                     gCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTaa                                             <  2:2113236/151‑1 (MQ=255)
                                                                                                     gCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTaa                                             <  1:3874998/151‑1 (MQ=255)
                                                                                                       ttCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAAcc                                           <  1:4726122/151‑1 (MQ=255)
                                                                                                         cACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTg                                         <  2:1923931/151‑1 (MQ=255)
                                                                                                         cACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTg                                         >  1:2684560/1‑151 (MQ=255)
                                                                                                            gggTGTCCAGCTTGGCGAGCGCGGCCAGGGCAGGGGGCAAGGACAGCGGGTGGGTGTCCGGCCCGGCGACGCCGAGTTGGCGCAACAGGGGGGGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGt                                      <  2:4451183/151‑1 (MQ=255)
                                                                                                             ggTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTa                                     >  1:1073956/1‑151 (MQ=255)
                                                                                                               tgtCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCCGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGCGCGCCGACGCTGCCCAGGGGACGGCAGCGCTTGAGGGTAACCGGAGCATg                                   >  2:5045731/1‑151 (MQ=255)
                                                                                                                gtCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATgg                                  <  2:3931402/151‑1 (MQ=255)
                                                                                                                 tCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATGGt                                 >  2:3298163/1‑151 (MQ=255)
                                                                                                                  ccAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATGGtt                                <  2:5229839/151‑1 (MQ=255)
                                                                                                                  ccAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATGGtt                                >  1:3628118/1‑151 (MQ=255)
                                                                                                                  ccAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATGGtt                                <  2:2381159/151‑1 (MQ=255)
                                                                                                                     gCTTGGCCAGCACTTCAAGGCCAGTGGCCAGGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATGGTTGCg                             <  1:3468330/151‑1 (MQ=255)
                                                                                                                       ttGGCCAGCACTTCAAGGCCAGTGGCCAGGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATGGTTGCGGc                           >  1:3196374/1‑151 (MQ=255)
                                                                                                                        tGGCCAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATGGTTGCGGCa                          <  1:260254/151‑1 (MQ=255)
                                                                                                                           ccAGCACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATGGTTGCGGCAAcc                       <  2:3019085/151‑1 (MQ=255)
                                                                                                                               cACTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATGGTTGCGGCAACCGTCg                   <  2:4669556/151‑1 (MQ=255)
                                                                                                                                 cTTCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATGGTTGCGGCAACCGTCGAg                 >  2:2918362/1‑151 (MQ=255)
                                                                                                                                   tCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCCCAGGGTACGGAAGCGCGTGAGGGTAACCTGAGTATGGTTGCGGCAACCGTCGGGGa               >  2:2833627/1‑151 (MQ=255)
                                                                                                                                   tCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATGGTTGCGGCAACCGTCGAGGa               <  1:2909744/151‑1 (MQ=255)
                                                                                                                                   tCAAGGCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATGGTTGCGGCAACCGTCGAGGa               >  1:955259/1‑151 (MQ=255)
                                                                                                                                       ggCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCATGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATGGTTGCGGCAACCGTCGAGGAAGGt           <  2:3257035/151‑1 (MQ=255)
                                                                                                                                        gCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATGGTTGCGGCAACCGTCGAGGAAGGTg          >  1:2366101/1‑151 (MQ=255)
                                                                                                                                        gCCAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATGGTTGCGGCAACCGTCGAGGAAGGTg          >  1:4373550/1‑151 (MQ=255)
                                                                                                                                         ccAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATGGTTGCGGCAACCGTCGAGGAAGGTGc         >  2:2195337/1‑151 (MQ=255)
                                                                                                                                         ccAGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATGGTTGCGGCAACCGTCGAGGAAGGTGc         >  2:813014/1‑151 (MQ=255)
                                                                                                                                          cAGTGGCCAGGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATGGTTGCGGCAACCGTCGAGGAAGGTGcc        <  2:4580372/151‑1 (MQ=255)
                                                                                                                                           aGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATGGTTGCGGCAACCGTCGAGGAAGGTGCCg       <  1:468506/151‑1 (MQ=255)
                                                                                                                                           aGTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATGGTTGCGGCAACCGTCGAGGAAGGTGCCg       <  1:4804372/151‑1 (MQ=255)
                                                                                                                                            gTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATGGTTGCGGCAACCGTCGAGGAAGGTGCCGa      <  1:5219680/151‑1 (MQ=255)
                                                                                                                                            gTGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATGGTTGCGGCAACCGTCGAGGAAGGTGCCGa      <  1:3727141/151‑1 (MQ=255)
                                                                                                                                             tGGCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATGGTTGCGGCAACCGTCGAGGAAGGTGCCGaa     <  1:435174/151‑1 (MQ=255)
                                                                                                                                              ggCCAGGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATGGTTGCGGCAACCGTCGAGGAAGGTGCCGAAg    <  2:5199747/151‑1 (MQ=255)
                                                                                                                                               gCCAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATGGTTGCGGCAACCGTCGAGGAAGGTGCCGAAgg   <  1:1990791/151‑1 (MQ=255)
                                                                                                                                                ccAAGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATGGTTGCGGCAACCGTCGAGGAAGGTGCCGAAGgt  <  2:2838442/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CATCGGGCAACAGCTCGCTCAACGCCTTAGCCGCCTCTGCCGGCACTAGTGCATCGCTGCCGGCGAACAGGTGCAGTTGCGGGCCATCATAGGCCTGCAAGGCTTCACGGGTGTCCAGCTTGGCCAGCACTTCAAGGCCAGTGGCCAGGTACAACGGGTCGGTATCCGGCACGCCGACACCCAGTTGGCGCAACAGGGTGCGCGGCTGCAGGGCGCCGTCGCTGCACAGGGTACGGAAGCGCTTGAGGGTAACCTGAGTATGGTTGCGGCAACCGTCGAGGAAGGTGCCGAAGGT  >  CP009974/1662197‑1662491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑