Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 1,678,037 C→T L212L (CTC→CTT)  RPPX_07515 → TonB‑dependent receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099741,678,0370CT99.3% 1126.4 / NA 292L212L (CTC→CTT) RPPX_07515TonB‑dependent receptor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (168/122);  minor base G (0/2);  total (168/124)
Fisher's exact test for biased strand distribution p-value = 1.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 292 total aligned reads displayed.

CGCCACCCGCGAGCAAATGAAAGACCGCGGTGTACACAGCCTGGATGATGCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTCGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGACCCCACCTGGCGGCCTGCTGGACATGGTCAGCCGCCGC  >  CP009974/1677890‑1678184
                                                                                                                                                   |                                                                                                                                                   
cGCCACCCGCGAGCAAATGAAAGACCGCGGTGTACACAGCCTGGATGATGCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGAt                                                                                                                                                  >  2:446479/1‑151 (MQ=255)
  ccACCCGCGAGCAAATGAAAGACCGCGGTGTACACAGCCTGGATGATGCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATgg                                                                                                                                                >  2:2149389/1‑151 (MQ=255)
    aCCCGCGAGCAAATGAAAGACCGCGGTGTACACAGCCTGGATGATGCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGcc                                                                                                                                              <  2:1208917/151‑1 (MQ=255)
     cccGCGAGCAAATGAAAGACCGCGGTGTACACAGCCTGGATGATGCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCt                                                                                                                                             <  2:5126267/151‑1 (MQ=255)
        gcgAGCAAATGAAAGACCGCGGTGTACACAGCCTGGATGATGCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGcc                                                                                                                                          >  1:406824/1‑151 (MQ=255)
           aGCAAATGAAAGACCGCGGTGTACACAGCCTGGATGATGCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCt                                                                                                                                       <  2:3531642/151‑1 (MQ=255)
           aGCAAATGAAAGACCGCGGTGTACACAGCCTGGATGATGCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCt                                                                                                                                       <  1:4474420/151‑1 (MQ=255)
           aGCAAATGAAAGACCGCGGTGTACACAGCCTGGATGATGCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCt                                                                                                                                       >  2:1090270/1‑151 (MQ=255)
            gCAAATGAAAGACCGCGGTGTACACAGCCTGGATGATGCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTg                                                                                                                                      >  1:2591199/1‑151 (MQ=255)
             cAAATGAAAGACCGCGGTGTACACAGCCTGGATGATGCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGc                                                                                                                                     <  2:668118/151‑1 (MQ=255)
                  gAAAGACCGCGGTGTACACAGCCTGGATGATGCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCcgc                                                                                                                                <  2:1999632/151‑1 (MQ=255)
                   aaaGACCGCGGTGTACACAGCCTGGATGATGCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCcgcg                                                                                                                               <  2:2603730/151‑1 (MQ=255)
                     aGACCGCGGTGTACACAGCCTGGATGATGCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCgcggc                                                                                                                             >  2:2200762/1‑151 (MQ=255)
                          gcgGTGTACACAGCCTGGATGATGCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTa                                                                                                                        >  1:2009478/1‑151 (MQ=255)
                          gcgGTGTACACAGCCTGGATGATGCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTa                                                                                                                        <  1:4519092/151‑1 (MQ=255)
                           cgGTGTACACAGCCTGGATGATGCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTAc                                                                                                                       >  1:2418907/1‑151 (MQ=255)
                            ggTGTACACAGCCTGGATGATGCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACg                                                                                                                      >  2:1442159/1‑151 (MQ=255)
                             gtgtACACAGCCTGGATGATGCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGc                                                                                                                     >  1:3230533/1‑151 (MQ=255)
                              tgtACACAGCCTGGATGATGCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGcc                                                                                                                    >  2:1976227/1‑151 (MQ=255)
                               gtACACAGCCTGGATGATGCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGccc                                                                                                                   >  1:248803/1‑150 (MQ=255)
                                    caGCCTGGATGATGCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCg                                                                                                              >  1:3929526/1‑151 (MQ=255)
                                     aGCCTGGATGATGCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGa                                                                                                             >  1:666758/1‑151 (MQ=255)
                                      gCCTGGATGATGCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGaa                                                                                                            >  2:2585972/1‑151 (MQ=255)
                                       ccTGGATGATGCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGaaa                                                                                                           <  2:1393188/151‑1 (MQ=255)
                                        cTGGATGATGCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAg                                                                                                          >  2:3937114/1‑151 (MQ=255)
                                          ggATGATGCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGcc                                                                                                        >  2:2737412/1‑151 (MQ=255)
                                            atgatgCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGa                                                                                                      >  1:3683653/1‑151 (MQ=255)
                                                 gCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCt                                                                                                 >  2:3956618/1‑151 (MQ=255)
                                                 gCAGTGCGCGACATGCCAGGCATCGTCTCCGCCAGCTCCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGGCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCt                                                                                                 <  2:4101595/151‑1 (MQ=255)
                                                  cAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTg                                                                                                >  1:3357770/1‑151 (MQ=255)
                                                      gcgcTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAAc                                                                                            <  1:699736/151‑1 (MQ=255)
                                                       cgcTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAAcc                                                                                           <  1:3288119/151‑1 (MQ=255)
                                                       cgcTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAAcc                                                                                           >  1:2722006/1‑151 (MQ=255)
                                                        gcTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCt                                                                                          >  1:3376666/1‑151 (MQ=255)
                                                          tACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCg                                                                                        >  1:616317/1‑151 (MQ=255)
                                                           aCATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTAGAACCTCGa                                                                                       >  2:5078075/1‑151 (MQ=255)
                                                           aCATGCCAGGCATCGGCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGa                                                                                       <  2:2457590/151‑1 (MQ=255)
                                                            cATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGAc                                                                                      >  1:4788438/1‑151 (MQ=255)
                                                            cATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGAc                                                                                      >  2:407530/1‑151 (MQ=255)
                                                             aTGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGAcc                                                                                     >  1:865183/1‑151 (MQ=255)
                                                                 cAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCt                                                                                 >  2:4386406/1‑151 (MQ=255)
                                                                      aTCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGccc                                                                            >  1:973906/1‑151 (MQ=255)
                                                                      aTCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGccc                                                                            >  2:2320521/1‑151 (MQ=255)
                                                                      aTCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGccc                                                                            >  2:1785790/1‑151 (MQ=255)
                                                                       tCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCct                                                                           <  2:1856896/151‑1 (MQ=255)
                                                                          tctcCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCctgct                                                                        <  2:462967/151‑1 (MQ=255)
                                                                           ctcCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCctgctg                                                                       <  1:4137988/151‑1 (MQ=255)
                                                                            tcCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCtgctgc                                                                      <  1:4407241/151‑1 (MQ=255)
                                                                            tcCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCtgctgc                                                                      <  2:1128387/151‑1 (MQ=255)
                                                                             ccGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTCCGCCAACCCGAGAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTgcg                                                                     >  1:2689254/1‑151 (MQ=255)
                                                                               gCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGcgcg                                                                   <  2:1571072/151‑1 (MQ=255)
                                                                                  aGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGcggcc                                                                <  2:4849117/151‑1 (MQ=255)
                                                                                     ttCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCGGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGcggcccgg                                                             <  2:48014/151‑1 (MQ=255)
                                                                                       cGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGcggcccggcc                                                           <  1:3614527/151‑1 (MQ=255)
                                                                                        ggCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGcggcccggccc                                                          >  2:2971589/1‑150 (MQ=255)
                                                                                          cAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCctct                                                        >  2:3804297/1‑151 (MQ=255)
                                                                                           aGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCtt                                                       <  1:4976283/151‑1 (MQ=255)
                                                                                           aGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCtt                                                       <  2:2503733/151‑1 (MQ=255)
                                                                                            gCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTc                                                      <  2:810410/151‑1 (MQ=255)
                                                                                             ggACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGAGGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCg                                                     <  2:604562/150‑1 (MQ=255)
                                                                                              gACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGa                                                    >  1:756619/1‑151 (MQ=255)
                                                                                              gACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGa                                                    <  2:4471224/151‑1 (MQ=255)
                                                                                                   ccGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTAc                                               <  2:2924373/151‑1 (MQ=255)
                                                                                                      ttATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGc                                            <  1:4605120/151‑1 (MQ=255)
                                                                                                      ttATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGc                                            >  1:2851640/1‑151 (MQ=255)
                                                                                                        aTGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGcc                                                                             <  1:4676426/116‑1 (MQ=255)
                                                                                                        aTGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGcc                                                                             >  2:4676426/1‑116 (MQ=255)
                                                                                                        aTGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCa                                          >  2:3306286/1‑151 (MQ=255)
                                                                                                         tGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAg                                         >  1:1588402/1‑151 (MQ=255)
                                                                                                            cTGGATGCGCGTGCGCGGCTTCGAGCCAACGCGGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCATCCCGGAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGAcc                                      <  2:4432510/151‑1 (MQ=255)
                                                                                                            cTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGAcc                                      <  1:4283251/151‑1 (MQ=255)
                                                                                                            cTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGAcc                                      >  1:1091168/1‑151 (MQ=255)
                                                                                                             tGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGAccc                                     >  1:1190525/1‑151 (MQ=255)
                                                                                                             tGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGAccc                                     <  1:228016/151‑1 (MQ=255)
                                                                                                              ggATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGAcccc                                    <  1:4821986/151‑1 (MQ=255)
                                                                                                              ggATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGAcccc                                    >  1:1514000/1‑151 (MQ=255)
                                                                                                                   cgcgtgcgcgGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGACCCCACCTg                               <  1:2520931/151‑1 (MQ=255)
                                                                                                                      gtgcgcgGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGACCCCACCTggcg                            <  2:2541788/151‑1 (MQ=255)
                                                                                                                       tgcgcgGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGACCCCACCTggcgg                           <  2:3994723/151‑1 (MQ=255)
                                                                                                                        gcgcgGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGACCCCACCTggcggc                          >  1:1801460/1‑151 (MQ=255)
                                                                                                                          gcgGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGACCCCACCTGGCGGCct                        >  1:1962185/1‑151 (MQ=255)
                                                                                                                          gcgGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGACCCCACCTGGCGGCct                        <  1:5065702/151‑1 (MQ=255)
                                                                                                                           cgGCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGACCCCACCTGGCGGCctg                       >  1:2422082/1‑151 (MQ=255)
                                                                                                                             gCTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCCCGGCCCGGCCTCTTCGATCTACGGCCAGACCCCACCTGGCGGCctgct                     >  1:1033408/1‑151 (MQ=255)
                                                                                                                              cTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGACCCCACCTGGCGGCctgctg                    <  1:1725250/151‑1 (MQ=255)
                                                                                                                              cTTCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGACCCCACCTGGCGGCctgctg                    >  2:73439/1‑151 (MQ=255)
                                                                                                                                tCGAGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCCGACCCCACCTGGCCGCCTCCTGGa                  >  2:4007832/1‑151 (MQ=255)
                                                                                                                                   aGCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGACCCCCCCTGGCGGCCTGCTGCACAt               >  2:3734842/1‑151 (MQ=255)
                                                                                                                                    gCCAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGACCCCACCTGGCGGCCTGCTGGACATg              <  1:3513008/151‑1 (MQ=255)
                                                                                                                                      cAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGACCCCACCTGGCGGCCTGCTGGACATGGt            <  1:2801184/151‑1 (MQ=255)
                                                                                                                                      cAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGACCCCACCTGGCGGCCTGCTGGACATGGt            >  1:4241565/1‑151 (MQ=255)
                                                                                                                                      cAACGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCCCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCCTCGATCTACGGCCAGACCCCACCTCGCGGCCTCCTCGACATggc            >  2:28551/1‑150 (MQ=255)
                                                                                                                                       aaCGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGACCCCACCTGGCGGCCTGCTGGACATGGTc           <  2:3460646/151‑1 (MQ=255)
                                                                                                                                        aCGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGACCCCACCTGGCGGCCTGCTGGACATGGTCa          >  1:2776344/1‑151 (MQ=255)
                                                                                                                                         cGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGACCCCACCTGGCGGCCTGCTGGACATGGTCAg         <  2:2658571/151‑1 (MQ=255)
                                                                                                                                         cGCAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGACCCCACCTGGCGGCCTGCTGGACATGGTCAg         <  1:5137621/151‑1 (MQ=255)
                                                                                                                                           cAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGACCCCACCTGGCGGCCTGCTGGACATGGTCAgcc       <  1:1317605/151‑1 (MQ=255)
                                                                                                                                           cAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGACCCCACCTGGCGGCCTGCTGGACATGGTCAgcc       <  1:2378651/151‑1 (MQ=255)
                                                                                                                                           cAGTTCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGACCCCACCTGGCGGCCTGCTGGACATGGTCAgcc       >  1:287892/1‑151 (MQ=255)
                                                                                                                                              ttCCTTGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGACCCCACCTGGCGGCCTGCTGGACATGGTCAgccgcc    <  2:2422082/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGCCACCCGCGAGCAAATGAAAGACCGCGGTGTACACAGCCTGGATGATGCAGTGCGCTACATGCCAGGCATCGTCTCCGCCAGCTTCGGCAGCGACACCCGTTATGACTGGATGCGCGTGCGCGGCTTCGAGCCAACGCAGTTCCTCGATGGCCTGCCGCTGCCCCGCGGCGTGTACGCCAACCCGAAAGCCGAAACCTGGAACCTCGACCGCCTCGCCCTGCTGCGCGGCCCGGCCTCTTCGATCTACGGCCAGACCCCACCTGGCGGCCTGCTGGACATGGTCAGCCGCCGC  >  CP009974/1677890‑1678184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑