Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 1,154,773 2 bp→GG coding (1473‑1474/2580 nt) RPPX_05115 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099741,154,7730AG99.0% 324.4 / NA 102A491A (GCA→GCG) RPPX_05115hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (55/46);  total (55/47)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0099741,154,7740CG99.0% 332.2 / ‑4.8 102L492V (CTG→GTG) RPPX_05115hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (55/46);  total (55/47)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 102 total aligned reads displayed.

TCAGCCAGGCGAGCAGTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCACTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACGCTGATTGGCAGTG  >  CP009974/1154713‑1154918
                                                            ||                                                                                                                                                
acAGCCAGGCGAGCAGTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACcgcg                                                         <  1:367960/150‑1 (MQ=11)
acAGCCAGGCGAGCAGTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACcgcg                                                         <  1:1666933/150‑1 (MQ=2)
acAGCCAGGCGAGCAGTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACcgcg                                                         >  2:2795787/2‑151 (MQ=2)
 cAGCCAGGCGAGCAGTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCgcgc                                                        >  1:1037988/1‑151 (MQ=9)
 cAGCCAGGCGAGCAGTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCgcgc                                                        >  2:4613008/1‑151 (MQ=9)
  aGCCAGGCGAGCAGTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCgcc                                                       <  1:2772523/151‑1 (MQ=9)
  aGCCAGGCGAGCAGTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCgcc                                                       >  2:1628024/1‑151 (MQ=9)
    ccAGGCGAGCAGTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGACGACGGCAGCAGCATCAGCGGTACCGCgccgg                                                     >  2:4364224/1‑151 (MQ=255)
    ccAGGCGAGCAGTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCgccgg                                                     <  1:2064666/151‑1 (MQ=16)
     cAGGCGAGCAGTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCgccggc                                                    >  1:5158333/1‑151 (MQ=16)
     cAGGCGAGCAGTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCgccggc                                                    >  1:2505980/1‑151 (MQ=16)
      aGGCGAGCAGTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCgccggcc                                                   >  1:229163/1‑151 (MQ=19)
      aGGCGAGCAGTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCgccggcc                                                   >  1:4580095/1‑151 (MQ=19)
       ggCGAGCAGTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCgccggccg                                                  <  2:1521766/151‑1 (MQ=255)
       ggCGAGCAGTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTGCCTCGGCGGTGAAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCgccggccg                                                  <  1:3195661/151‑1 (MQ=255)
        gCGAGCAGTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGccggccgg                                                 <  1:2528469/151‑1 (MQ=255)
         cGAGCAGTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCcggccggc                                                <  2:1541116/151‑1 (MQ=255)
          gAGCAGTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCa                                               >  1:1897380/1‑151 (MQ=25)
          gAGCAGTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCa                                               <  2:1173792/151‑1 (MQ=255)
          gAGCAGTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCa                                               <  1:1141347/151‑1 (MQ=255)
           ggCAGTTGGGCCTGGTGCAGACCGACCCAGGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCCCCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGGGCCGGCCGGCAc                                              <  2:940386/150‑1 (MQ=11)
           aGCAGTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCAc                                              <  2:2730973/151‑1 (MQ=255)
             cAGTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCAccc                                            <  2:345904/151‑1 (MQ=255)
             cAGTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCAccc                                            <  2:320436/151‑1 (MQ=255)
              aGTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCCGCGGTACCGCGCCGGCCGGCACCCg                                           >  2:4751770/1‑151 (MQ=255)
               gTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCgt                                          <  2:5150546/151‑1 (MQ=255)
               gTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCgt                                          <  2:1293462/151‑1 (MQ=255)
               gTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCgt                                          >  1:2652523/1‑151 (MQ=255)
               attGAGCCGGGTACGGACCGACCCAAGCGGCAACGCTTCCTCGGCACTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCgt                                          <  2:214287/150‑1 (MQ=255)
                 tGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCgtgt                                        <  1:3105261/151‑1 (MQ=255)
                 tGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCgtgt                                        >  2:2283482/1‑151 (MQ=255)
                 tGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCgtgt                                        <  1:3353665/151‑1 (MQ=255)
                  gAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGtgtg                                       >  2:1052140/1‑151 (MQ=255)
                   aGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTgg                                      <  2:2527531/151‑1 (MQ=255)
                    gCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGa                                     >  1:3811290/1‑151 (MQ=255)
                    gCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGa                                     >  1:1355331/1‑151 (MQ=255)
                     ccTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGaa                                    >  1:2676257/1‑151 (MQ=255)
                      cTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAg                                   >  1:4794611/1‑151 (MQ=255)
                      cTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAg                                   >  2:1929671/1‑151 (MQ=255)
                       tGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGt                                  <  2:352153/151‑1 (MQ=255)
                       tGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGt                                  >  1:586337/1‑151 (MQ=255)
                       tGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGt                                  <  1:1392777/151‑1 (MQ=255)
                       tGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGt                                  >  1:1037777/1‑151 (MQ=255)
                       tGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGCGTGGAAGt                                  >  2:2954333/1‑151 (MQ=255)
                         gcgcgccccgcccCAAGCGGCAACGCTTCCTCGGCGGTGCACTTCGACGTCCCGGCCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGc                                <  2:3581374/144‑1 (MQ=11)
                         gTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGc                                <  2:3678021/151‑1 (MQ=255)
                         gTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGGGGAAGTGc                                >  2:3964645/1‑151 (MQ=255)
                         gTGCAGACCGACCCAAGCGGCAACGCTTCCGCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGc                                <  2:4399070/151‑1 (MQ=255)
                           gCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCAt                              <  1:1383319/151‑1 (MQ=255)
                           gCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCAt                              <  2:2673586/151‑1 (MQ=255)
                           gCAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCAt                              >  1:2874698/1‑151 (MQ=255)
                            cAGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATg                             >  1:4895685/1‑151 (MQ=255)
                             aGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGa                            >  1:4189400/1‑151 (MQ=255)
                             aGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGa                            >  1:3045078/1‑151 (MQ=255)
                             aGACCGACCCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGa                            >  2:3737789/1‑151 (MQ=255)
                              gaccgaccCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGAc                           <  2:2272100/151‑1 (MQ=255)
                              gaccgaccCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGAc                           <  1:1270387/151‑1 (MQ=255)
                              gaccgaccCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGAc                           >  1:3266259/1‑151 (MQ=255)
                              gaccgaccCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGAc                           >  1:1169053/1‑151 (MQ=255)
                                ccgaccCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGc                         >  1:885212/1‑151 (MQ=255)
                                ccgaccCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGc                         >  1:3017917/1‑151 (MQ=255)
                                ccgaccCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGc                         <  1:4408425/151‑1 (MQ=255)
                                 cgaccCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGcc                        >  2:2303841/1‑151 (MQ=255)
                                  gaccCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCa                       >  1:80952/1‑151 (MQ=255)
                                  gaccCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCa                       <  1:2452105/151‑1 (MQ=255)
                                  gaccCAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGCGCGGAAGTGCATGACGCCa                       >  2:5209345/1‑151 (MQ=255)
                                    cccAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAAc                     <  1:3333421/151‑1 (MQ=255)
                                     ccAAGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACg                    >  2:2705010/1‑151 (MQ=255)
                                     ccAAGCGGCAACGCTGCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACg                    <  2:903589/151‑1 (MQ=255)
                                       aaGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGTTTGACGCCAACGGc                  >  2:1801840/1‑151 (MQ=255)
                                       aaGCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCGAACGGc                  >  2:4540049/1‑151 (MQ=255)
                                         gCGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACGGCAc                >  2:3931972/1‑151 (MQ=255)
                                          cGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACg               >  2:2331567/1‑151 (MQ=255)
                                          cGGCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACg               >  1:3048681/1‑151 (MQ=255)
                                           ggCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACGc              >  1:4543339/1‑151 (MQ=255)
                                           ggCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACGc              >  2:5212778/1‑151 (MQ=255)
                                           ggCAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACGc              >  1:543381/1‑151 (MQ=255)
                                             cAACGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCCACGGCACGCTg            >  2:3007477/1‑151 (MQ=255)
                                              aaCGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATTACGCCAACTGCACGCTGa           >  1:3466434/1‑151 (MQ=255)
                                              aaCGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACGCTGa           >  2:2059058/1‑151 (MQ=255)
                                              aaCGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACGCTGa           >  1:432032/1‑151 (MQ=255)
                                               aCGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACGCTGAt          <  1:4139195/151‑1 (MQ=255)
                                               aCGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACGCTGAt          <  1:938278/151‑1 (MQ=255)
                                               aCGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGCGTGGAAGTGCACGACGCCAACGGCACGCTGAt          >  2:2640509/1‑151 (MQ=255)
                                                cGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACGCTGAtt         >  1:1172183/1‑151 (MQ=255)
                                                cGCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACAACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACGCTGAtt         <  1:697747/151‑1 (MQ=255)
                                                 gcgtCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACGCTGATTg        <  1:2094786/148‑1 (MQ=255)
                                                 gCTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACGCTGATTg        <  1:344247/151‑1 (MQ=255)
                                                 gCTGCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCGGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACGCTGATTg        <  2:3184176/151‑1 (MQ=255)
                                                 ccTTCCTCGTCGGTGCAACTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCACAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACGCTGATTg        <  1:343931/150‑1 (MQ=14)
                                                  cTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGCAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCAAACGGCACGCTGATTgg       >  2:1817475/1‑151 (MQ=255)
                                                  cTTCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACGCTGATTgg       >  1:2635082/1‑151 (MQ=255)
                                                   ttCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACGCTGATTGGc      >  1:3303062/1‑151 (MQ=255)
                                                   ttCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACGCTGATTGGc      >  2:1938075/1‑151 (MQ=255)
                                                   ttCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACGCTGATTGGc      <  1:892136/151‑1 (MQ=255)
                                                    tCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCGGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACGCTGATTGGCa     <  1:88329/151‑1 (MQ=255)
                                                    tCCTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACGCTGATTGGCa     <  1:88433/151‑1 (MQ=255)
                                                    cccTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACGCTGATTGGCa     >  1:3097075/2‑151 (MQ=255)
                                                     ccTCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACGCTGATTGGCAg    <  1:758921/151‑1 (MQ=255)
                                                       tCGGCGGTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACGCTGATTGGCAgtg  <  2:3688720/151‑1 (MQ=255)
                                                            ||                                                                                                                                                
TCAGCCAGGCGAGCAGTTGAGCCTGGTGCAGACCGACCCAAGCGGCAACGCTTCCTCGGCACTGCAATTCGAAGTCCCGGTCACCACTGCGCCTGCCAGCCCCAGCGACCTGGTGTTCGCCGAAGACGGCAGCAGCATCAGCGGTACCGCGCCGGCCGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACGCTGATTGGCAGTG  >  CP009974/1154713‑1154918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑