BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	1,358,424	2 bp→TC	intergenic (+285/+2404)	RPPX_05970 → / ← RPPX_05980	hypothetical protein/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	1,358,424	0	C	T	98.8%	618.3 / NA	247	intergenic (+285/+2405)	RPPX_05970/RPPX_05980	hypothetical protein/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base C (1/1); major base T (141/100); minor base G (2/1); total (144/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP009974	1,358,425	0	T	C	99.6%	619.1 / NA	247	intergenic (+286/+2404)	RPPX_05970/RPPX_05980	hypothetical protein/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (144/102); minor base G (0/1); total (144/103)
Fisher's exact test for biased strand distribution p-value = 4.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 247 total aligned reads displayed.

CATTGAGCCAATGAGTTCCTCCCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCGGGACTGGCGGCTCCTTACGTCCGGGAGCAAGGGCATCTCATGATCTGGCCTCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCTTGGTCTG  >  CP009974/1358279‑1358569

||

cATTGAGCCAATGAGTTCCTCCCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTg                                                                                                                                              <  2:373469/151‑1 (MQ=25)

cATTGAGCCAATGAGTTCCTCCCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTg                                                                                                                                              >  1:2099204/1‑151 (MQ=25)

     aGCCAATGAGTTCCTCCCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCAc                                                                                                                                         <  2:233811/151‑1 (MQ=25)

     aGCCAATGAGTTCCTCCCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCAc                                                                                                                                         >  1:3827046/1‑151 (MQ=25)

      gCCAATGAGTTCCTCCCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACTGTGAGCAAGGGCATCTCATGAGCTGGCTCCCTGAGCGcc                                                                                                                                        >  2:1508586/1‑151 (MQ=255)

        cAATGAGTTCCTCCCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGt                                                                                                                                      >  1:5126176/1‑151 (MQ=21)

        cAATGAGTTCCTCCCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGt                                                                                                                                      >  1:2308346/1‑151 (MQ=21)

         aaTGAGTTCCTCCCAAGATCTAGTCCTAGCACAGACCTTATTTATGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGtt                                                                                                                                     >  1:3948192/1‑151 (MQ=21)

           tGAGTTCCTCCCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTAc                                                                                                                                   <  1:3509517/151‑1 (MQ=21)

             aGTTCCTCCCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCg                                                                                                                                 >  2:4038487/1‑151 (MQ=21)

             aGTTCCTCCCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCCGAGCACCGTTCCCg                                                                                                                                 >  2:4389181/1‑151 (MQ=21)

               ttCCTCCCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGt                                                                                                                               >  2:1305507/1‑151 (MQ=21)

                 cctccCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTgg                                                                                                                             >  1:1580716/1‑151 (MQ=21)

                 cctccCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTgg                                                                                                                             <  2:1255846/151‑1 (MQ=21)

                  ctccCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTggg                                                                                                                            >  1:1229207/1‑151 (MQ=21)

                   tccCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGc                                                                                                                           >  2:2774002/1‑151 (MQ=21)

                      cAAGATCTAGTGCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCggg                                                                                                                        <  2:4545651/151‑1 (MQ=18)

                       aaGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGt                                                                                                                       >  1:4021753/1‑151 (MQ=18)

                         gATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGTCGGGTgg                                                                                                                     >  2:3223727/1‑151 (MQ=18)

                         gATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTgg                                                                                                                     >  1:1775301/1‑151 (MQ=18)

                          aTCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGa                                                                                                                    <  2:3559536/151‑1 (MQ=18)

                          aTCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGa                                                                                                                    <  2:2905480/151‑1 (MQ=18)

                             tAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGc                                                                                                                 >  2:2612867/1‑151 (MQ=18)

                             tAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGc                                                                                                                 >  2:3321366/1‑151 (MQ=18)

                               gTCCTCGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTg                                                                                                               <  2:4766362/151‑1 (MQ=17)

                               gTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTg                                                                                                               <  1:2543211/151‑1 (MQ=18)

                               gTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTg                                                                                                               >  1:4466957/1‑151 (MQ=18)

                                tCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGTGCATCTCATGATCTGGCTCACTGAGCACCGTTACCGGGTGGCGGGCGGAGGCAGc                                                                                                              >  2:3702286/1‑151 (MQ=255)

                                 ccTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCa                                                                                                             <  2:4142864/151‑1 (MQ=18)

                                  cTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCAt                                                                                                            >  1:3099969/1‑151 (MQ=17)

                                  cTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCAt                                                                                                            >  2:2804404/1‑151 (MQ=18)

                                   tAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCAtt                                                                                                           >  1:4875472/1‑151 (MQ=17)

                                    aGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTg                                                                                                          <  2:1082112/151‑1 (MQ=18)

                                          gACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGAc                                                                                                    <  1:1024914/151‑1 (MQ=17)

                                          gACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGAc                                                                                                    <  1:57430/151‑1 (MQ=17)

                                          gACCTTATGTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGAc                                                                                                    <  2:3954558/151‑1 (MQ=17)

                                                   tAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGt                                                                                           <  1:2502242/151‑1 (MQ=17)

                                                   tAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGt                                                                                           >  1:3978143/1‑151 (MQ=17)

                                                    aGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACAGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGAACAGTg                                                                                          >  1:3383623/1‑151 (MQ=14)

                                                      ggATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGcc                                                                                        >  2:325569/1‑151 (MQ=17)

                                                        aTCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCcgc                                                                                      >  2:4582752/1‑151 (MQ=17)

                                                         tCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCcgcg                                                                                     >  2:597392/1‑151 (MQ=17)

                                                         tCAAGGCACATGCGAAATGTGGGAAATGTCCTCTACGCTGGGCTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGGGCACCGTTGACGGTGGGCGGGTGGCGGCTGCATTGGCTGACGAGACCAGGGCagcg                                                                                     <  2:2218249/151‑1 (MQ=14)

                                                           aaGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCgag                                                                                   >  1:2181000/1‑151 (MQ=17)

                                                           aaGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCgag                                                                                   >  2:1168188/1‑151 (MQ=17)

                                                            aGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCgaga                                                                                  <  1:5001078/151‑1 (MQ=17)

                                                            aGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCgaga                                                                                  <  1:651178/151‑1 (MQ=17)

                                                               cACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATcc                                                                               <  1:4787950/151‑1 (MQ=17)

                                                                aCCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGGCGAGACCAGTGCCGCGAGATCCt                                                                              >  2:5042960/1‑151 (MQ=14)

                                                                  cTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGCTCCTGa                                                                            >  1:1136295/1‑151 (MQ=14)

                                                                  cTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGa                                                                            <  2:1373719/151‑1 (MQ=14)

                                                                   tGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAg                                                                           >  1:800262/1‑151 (MQ=14)

                                                                     cGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGcc                                                                         <  1:2872223/151‑1 (MQ=14)

                                                                      gACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCg                                                                        <  1:120392/151‑1 (MQ=14)

                                                                       aCATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCgg                                                                       >  1:929788/1‑151 (MQ=14)

                                                                        cATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCggg                                                                      >  2:913913/1‑151 (MQ=14)

                                                                         aTGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGt                                                                     >  1:2678239/1‑151 (MQ=14)

                                                                            tgGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGtgca                                                                  <  2:4946987/151‑1 (MQ=14)

                                                                                 aaTGTCGTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATgcag                                                             <  2:5138544/151‑1 (MQ=14)

                                                                                  aTGTACTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATgcagc                                                            >  2:1783245/1‑151 (MQ=14)

                                                                                   tGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATgcagca                                                           <  2:2472082/151‑1 (MQ=14)

                                                                                     tCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACt                                                         >  2:2228160/1‑151 (MQ=14)

                                                                                       ctctACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGc                                                       >  2:2880513/1‑151 (MQ=14)

                                                                                        tctACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCg                                                      >  1:4069357/1‑151 (MQ=14)

                                                                                         ctACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGa                                                     >  2:645508/1‑151 (MQ=14)

                                                                                            cgctggACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGa                                                  <  2:1652552/147‑1 (MQ=14)

                                                                                             gctggACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGAc                                                 <  2:4681081/148‑1 (MQ=14)

                                                                                             gctggACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGAc                                                 <  1:5177650/148‑1 (MQ=255)

                                                                                              ctggACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGAca                                                >  2:2668755/3‑150 (MQ=14)

                                                                                              ctggACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGAca                                                <  2:1003857/149‑2 (MQ=14)

                                                                                                ggACTGGCGGCTCCTTAAGACCAGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAgg                                              <  2:255016/151‑1 (MQ=14)

                                                                                                   cTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGc                                           >  1:1865742/1‑151 (MQ=14)

                                                                                                   cTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGc                                           >  2:4188292/1‑151 (MQ=14)

                                                                                                      gcggcTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATg                                        <  2:1438453/151‑1 (MQ=14)

                                                                                                      gcggcTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATg                                        <  1:3244954/151‑1 (MQ=14)

                                                                                                        ggcTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGcc                                      >  1:4596264/1‑151 (MQ=14)

                                                                                                        ggcTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGcc                                      <  1:4103098/151‑1 (MQ=14)

                                                                                                         gcTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCt                                     <  1:1526057/151‑1 (MQ=14)

                                                                                                             cTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCCCTGCGATGACAGGGGCATGCCTGACt                                 >  1:3204733/1‑151 (MQ=14)

                                                                                                              ttaagaccGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTg                                >  1:703050/7‑151 (MQ=14)

                                                                                                                 agaccGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTgtca                             >  2:1772688/4‑151 (MQ=255)

                                                                                                                  gaccGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTgtcag                            <  2:1457447/149‑1 (MQ=255)

                                                                                                                  gaccGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTgtcag                            <  1:2842219/149‑1 (MQ=255)

                                                                                                                      gggAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGCGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGtcagtcag                        <  1:3075190/151‑1 (MQ=255)

                                                                                                                         ggCAAGGGCATCTCATGATGTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTg                     <  2:579379/150‑1 (MQ=255)

                                                                                                                           cAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCa                   <  2:3359594/151‑1 (MQ=255)

                                                                                                                            aaGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCAg                  >  1:3702997/1‑151 (MQ=255)

                                                                                                                             aGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCAGt                 >  2:1884289/1‑151 (MQ=255)

                                                                                                                             aGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCAGt                 >  1:3576720/1‑151 (MQ=255)

                                                                                                                                gCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTTAGGTGCAGTCCa              >  1:906359/1‑151 (MQ=255)

                                                                                                                                gCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCa              <  1:373701/151‑1 (MQ=255)

                                                                                                                                gCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCa              >  1:4262357/1‑151 (MQ=255)

                                                                                                                                gCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCa              <  1:1367009/151‑1 (MQ=255)

                                                                                                                                 cATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCAt             >  2:2011844/1‑151 (MQ=255)

                                                                                                                                   tctcGTGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTc           <  1:1676641/151‑1 (MQ=255)

                                                                                                                                   tctcATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTc           <  1:4612613/151‑1 (MQ=255)

                                                                                                                                       aTGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCTTg       >  1:1048399/1‑151 (MQ=255)

                                                                                                                                        tGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCTTgg      <  1:2000185/151‑1 (MQ=255)

                                                                                                                                        tGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCTTgg      >  2:842806/1‑151 (MQ=255)

                                                                                                                                          aTCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCTTGGTc    >  2:4351212/1‑151 (MQ=255)

||

CATTGAGCCAATGAGTTCCTCCCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCGGGACTGGCGGCTCCTTACGTCCGGGAGCAAGGGCATCTCATGATCTGGCCTCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCTTGGTCTG  >  CP009974/1358279‑1358569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`