Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	418,441	Δ1 bp	intergenic (‑20/+631)	RPPX_01715 ← / ← RPPX_01720	hypothetical protein/lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	418,441	0	A	.	100.0%	103.0 / NA	48	intergenic (‑20/+631)	RPPX_01715/RPPX_01720	hypothetical protein/lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (21/27);  total (21/27)

TGTTGCTGTTGCTGTTGCTGTTGCTGTTGCTGTTGCTGTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGC‑GTACTCCCGGGA  >  CP009974/418295‑418479                                                                                                                                                   |                                        tgttgctgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGccc                                    >  1:793758/1‑151 (MQ=255) tgttgctgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGccc                                    >  1:202437/1‑151 (MQ=255)  gttgctgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGcccc                                   >  2:2632282/1‑151 (MQ=255)   ttgctgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCa                                  >  1:4443130/1‑151 (MQ=255)     gctgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCagg                                >  2:2441097/1‑149 (MQ=255)      ctgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCagga                               <  2:96502/151‑4 (MQ=255)       tgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCaggat                              >  2:2659055/1‑147 (MQ=14)       tgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCaggat                              <  1:2906707/151‑5 (MQ=14)       tgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCaggat                              >  1:4782010/1‑147 (MQ=14)       tgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGATGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCaggat                              >  1:3754622/1‑147 (MQ=11)       tgttgctgttgctgttgctgttgctgttgctgttgATCTTGATATTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGACGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCaggat                              <  2:2308271/151‑5 (MQ=11)        gttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCca‑ggatg                            >  1:1779741/1‑151 (MQ=14)        gttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCca‑ggatg                            >  1:2344765/1‑151 (MQ=14)         ttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCca‑ggatgg                           >  2:4106422/1‑151 (MQ=14)         ttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCca‑ggatgg                           >  1:1506658/1‑151 (MQ=14)          tgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATggg                          <  2:170193/151‑1 (MQ=11)           gctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATgggg                         <  2:2221042/151‑1 (MQ=11)           gctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATgggg                         >  1:4204985/1‑151 (MQ=11)           gctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATgggg                         >  1:1330301/1‑151 (MQ=11)            ctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGc                        <  1:1165225/151‑1 (MQ=11)            ctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGc                        <  1:1164919/151‑1 (MQ=11)            ctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGc                        >  1:1538576/1‑151 (MQ=11)               ttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTg                     <  2:133295/151‑1 (MQ=11)               ttgctgttgctgttgctgttgctgttgATCTTGATCTTGAGCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTg                     <  2:132057/151‑1 (MQ=11)                tgctgttgctgttgctgttgctgttgATCTTGAGCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGACCGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGc                    <  1:3330462/151‑1 (MQ=11)                 gctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCa                   >  2:3506308/1‑151 (MQ=14)                 gctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCa                   <  2:2670819/151‑1 (MQ=14)                  gtggtggtgtggctgttgctgttgGTGTTGATCTTGATCTTGATCTTGATATTGATCGGCGCGACTTCAGGAGGCCGAACGTAGGCCTGGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAg                  <  2:5180247/144‑1 (MQ=1)                  ctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCGCCA‑GGATGGGGCCTGCAg                  >  1:755766/1‑151 (MQ=14)                  ctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCtcagc                  <  1:2441097/151‑5 (MQ=11)                   tgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAgc                 <  2:1530892/151‑1 (MQ=14)                    gttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAgcg                <  1:4922080/151‑1 (MQ=14)                    gttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAgcg                >  2:6194109/1‑151 (MQ=14)                    gttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAgcg                >  2:1862200/1‑151 (MQ=14)                    gttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAgcg                <  1:5145710/151‑1 (MQ=14)                    ggtgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCGTCGCGACGTCAGGAGGCCGAACGTAGGCCTTGCGGCGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAgcg                <  2:5876530/149‑1 (MQ=14)                      tgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑g             <  1:4621128/151‑1 (MQ=14)                      tgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑g             <  1:4622147/151‑1 (MQ=14)                         tgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggtc          >  1:5596094/1‑148 (MQ=11)                         tgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggtc          <  1:4662012/151‑4 (MQ=11)                          gttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggtcc         <  1:3950988/151‑5 (MQ=11)                          gttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggtcc         <  1:5194262/151‑5 (MQ=11)                           ttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggtcct        <  1:497516/151‑6 (MQ=11)                            tgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCACGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggtcctc       <  1:3207654/151‑7 (MQ=11)                               tgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCg     >  2:4294264/1‑151 (MQ=11)                                gttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCgg    <  2:2093012/151‑1 (MQ=11)                                 ttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCggg   <  1:3867250/151‑1 (MQ=11)                                  tgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGa  <  1:6336114/151‑1 (MQ=11)                                                                                                                                                   |                                        TGTTGCTGTTGCTGTTGCTGTTGCTGTTGCTGTTGCTGTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGC‑GTACTCCCGGGA  >  CP009974/418295‑418479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑