Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 3,514,540 (G)6→5 coding (316/2754 nt) RPPX_15700 ← chemotaxis protein CheY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099743,514,5350G.98.8% 1796.1 / 7.0 342coding (321/2754 nt)RPPX_15700chemotaxis protein CheY
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base . (169/169);  total (170/172)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 377 total aligned reads displayed.

CAGCTCGATCTCTACCCAGCCCAGCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCTGGGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGCCATGGCGGGTG  >  CP009974/3514385‑3514683
                                                                                                                                                      |                                                                                                                                                    
  gCTCGATCTCTACCCAGCCCAGCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCTggg                                                                                                                                                    <  2:5846348/151‑1 (MQ=255)
  gCTCGATCTCTACCCAGCCCAGCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCTggg                                                                                                                                                    <  1:5588250/151‑1 (MQ=255)
      gATCTCTACCCAGCCCAGCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCTgggggca                                                                                                                                                >  1:2898796/1‑149 (MQ=255)
      gATCTCCACCCAGCCCAGCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCTGTACCGCCCCTgggggtc                                                                                                                                                >  1:1125237/1‑149 (MQ=255)
       aTCTCTACCCAGCCCAGCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCTgggggcag                                                                                                                                               <  2:5107981/151‑4 (MQ=255)
       aTCTCTACCCAGCCCAGCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCTgggggcag                                                                                                                                               >  1:2007876/1‑148 (MQ=255)
        tctcTACCCAGCCCAGCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCTgggggcagg                                                                                                                                              >  2:4581810/1‑147 (MQ=255)
        tctcTACCCAGCCCAGCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCTgggggcagg                                                                                                                                              <  1:5092686/151‑5 (MQ=255)
              cccagcccagCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGc                                                                                                                                       >  2:3263940/1‑151 (MQ=255)
              cccagcccagCAGGCGATGGGCTTCGGCGGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGc                                                                                                                                       <  1:1667484/151‑1 (MQ=255)
               ccagcccagcAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCa                                                                                                                                      >  2:2327834/1‑151 (MQ=255)
               ccagcccagcAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCa                                                                                                                                      <  1:3388601/151‑1 (MQ=255)
                   cccagcAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCt                                                                                                                                  <  1:6413025/151‑1 (MQ=255)
                   cccagcAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCt                                                                                                                                  >  1:2341558/1‑151 (MQ=255)
                   cccagcAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCt                                                                                                                                  <  2:355573/151‑1 (MQ=255)
                     cagcggGCGCTCGGCTGCGGCCGGGACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTg                                                                                                                                <  2:6355606/151‑1 (MQ=255)
                     cagcagGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTg                                                                                                                                >  2:5504144/1‑151 (MQ=255)
                       gcagGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTggg                                                                                                                              <  1:1786063/151‑1 (MQ=255)
                         agGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGcc                                                                                                                            >  1:4380190/1‑151 (MQ=255)
                               tCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTg                                                                                                                      >  1:5726159/1‑151 (MQ=255)
                                   cTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGcc                                                                                                                  >  1:4731975/1‑151 (MQ=255)
                                    ttCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGGGGGCCa                                                                                                                 >  2:6513011/1‑151 (MQ=255)
                                      cggccggcACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAgg                                                                                                               >  1:3186383/1‑151 (MQ=255)
                                        gccggcACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGgcg                                                                                                             <  1:1088988/151‑1 (MQ=255)
                                         ccggcACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGgcgc                                                                                                            <  1:101104/151‑1 (MQ=255)
                                          cggcACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGcgcg                                                                                                           >  1:4500600/1‑151 (MQ=255)
                                           ggcACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGCCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGt                                                                                                          <  1:1154185/151‑1 (MQ=255)
                                            gcACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTg                                                                                                         >  1:554181/1‑151 (MQ=255)
                                               cGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCgg                                                                                                      >  2:3197834/1‑151 (MQ=255)
                                                ggCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGt                                                                                                     >  1:253223/1‑151 (MQ=255)
                                                  cGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTcc                                                                                                   <  1:3959382/151‑1 (MQ=255)
                                                   gTCGGTGGCCGGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCCCTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCCGGGTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCg                                                                                                  <  1:2849662/151‑1 (MQ=255)
                                                   gTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCg                                                                                                  >  1:2554404/1‑151 (MQ=255)
                                                      ggTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCgggg                                                                                               <  2:4846917/151‑1 (MQ=255)
                                                      ggTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCgggg                                                                                               <  1:599607/151‑1 (MQ=255)
                                                      ggTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCgggg                                                                                               >  1:3678566/1‑151 (MQ=255)
                                                          gCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAg                                                                                           <  2:2158523/151‑1 (MQ=255)
                                                            cAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGaa                                                                                         <  1:1510503/151‑1 (MQ=255)
                                                               gTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACg                                                                                      >  1:5404623/1‑151 (MQ=255)
                                                               gTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACg                                                                                      <  2:682903/151‑1 (MQ=255)
                                                                  gcgGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCa                                                                                   >  1:876246/1‑151 (MQ=255)
                                                                    ggtggtGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACt                                                                                 <  1:4008095/151‑1 (MQ=255)
                                                                    ggtggtGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGAAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACt                                                                                 >  1:1875844/1‑151 (MQ=255)
                                                                     gtggtgGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTg                                                                                <  1:4466637/151‑1 (MQ=255)
                                                                     gtggtgGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTg                                                                                >  2:2627534/1‑151 (MQ=255)
                                                                      tggtggCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTgc                                                                               <  2:3953505/151‑1 (MQ=255)
                                                                      tggtggCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGCGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTgc                                                                               >  2:1707234/1‑151 (MQ=255)
                                                                        gtggCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTgcgc                                                                             >  1:1424917/1‑151 (MQ=255)
                                                                         tggCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTgcgc                                                                             <  1:1945914/150‑1 (MQ=255)
                                                                          ggCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGc                                                                                     <  1:3621139/141‑1 (MQ=255)
                                                                            ccGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGAGGCACGGGGCCACAGACGCCACTGCGCGcac                                                                         >  2:5024703/1‑151 (MQ=255)
                                                                             cGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGTGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGTGCCAGAAACGCCACTGCGCGcaca                                                                        >  2:2580742/1‑151 (MQ=255)
                                                                             cGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGcaca                                                                        >  1:5023049/1‑151 (MQ=255)
                                                                              gAACACTGGCATCCGGTACCAGGTGGCCTCGTTGCCACTGCGCTCCAGGAGCTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTCGGCCGGCGTGGGCCCGGCGCGCGCGGGGCGGGGCCCGCAGCGCCGCTGCGCGcactg                                                                       >  2:1498574/1‑149 (MQ=255)
                                                                               aaCACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATc                                                                      <  2:1963040/151‑1 (MQ=255)
                                                                               aaCACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATc                                                                      <  1:3122172/151‑1 (MQ=255)
                                                                                acacTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCg                                                                     <  2:4380190/151‑1 (MQ=255)
                                                                                 cacTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCgg                                                                    >  1:822915/1‑151 (MQ=255)
                                                                                  cctagCATCAGGTGGCGGGTGGCGTCGTTACCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGC‑GGGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGc                                                                   <  2:2870267/147‑1 (MQ=255)
                                                                                  acTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGc                                                                   >  2:1319653/1‑151 (MQ=255)
                                                                                   cTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGGGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGcc                                                                  <  2:1439/151‑1 (MQ=255)
                                                                                    tGGCATCAGGTAGCGGGGGGCGTCGTTGCCACTGCGCTGCAGGAGTCGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCt                                                                 <  2:5707850/151‑1 (MQ=255)
                                                                                     ggCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTg                                                                <  2:4468707/151‑1 (MQ=255)
                                                                                       cATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCt                                                              <  1:2436821/151‑1 (MQ=255)
                                                                                             gTAGCGGGTGGCGTCGTGGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAggg                                                        <  2:1807622/151‑1 (MQ=255)
                                                                                                gCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGttt                                                     <  2:3783932/151‑1 (MQ=255)
                                                                                                  gggTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTtgt                                                   <  1:2697276/151‑1 (MQ=255)
                                                                                                     tGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCg                                                >  2:2464362/1‑151 (MQ=255)
                                                                                                        cgtcgtTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCa                                             >  2:2372229/1‑151 (MQ=255)
                                                                                                        cgtcgtTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCa                                             >  1:4101544/1‑151 (MQ=255)
                                                                                                         gtcgtTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGGCACGCCACTGCGCGCACATCGGCCTGCTCCAGGGGGTGTGCGGcga                                            >  2:4118888/1‑149 (MQ=255)
                                                                                                            gtTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATtc                                         >  2:1014445/1‑151 (MQ=255)
                                                                                                            gtTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATtc                                         >  1:4299578/1‑151 (MQ=255)
                                                                                                             ttGCCGCGGCGCTGCAGGAGTTGGGTGCCGGTACCGGCGCT‑GGGGGCAGGCTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATtct                                        <  2:6278427/151‑1 (MQ=255)
                                                                                                               gCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATtctct                                      >  1:3260305/1‑151 (MQ=255)
                                                                                                               gCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATtctct                                      <  1:5288970/151‑1 (MQ=255)
                                                                                                                  aCTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCa                                   <  2:4299578/151‑1 (MQ=255)
                                                                                                                   cTGCGCTGCAGGAGGTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAg                                  <  2:5772319/151‑1 (MQ=255)
                                                                                                                     gcgcTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGtt                                >  2:1086562/1‑151 (MQ=255)
                                                                                                                     gcgcTGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGtt                                <  1:6182143/151‑1 (MQ=255)
                                                                                                                      ggcTGCAGGAGTGGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTg                               <  2:4683749/150‑1 (MQ=255)
                                                                                                                         tGCAGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTgcgc                            >  1:5929348/1‑151 (MQ=255)
                                                                                                                            aGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAgg                         >  2:6149602/1‑151 (MQ=255)
                                                                                                                            aGGAGTTGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAgg                         >  2:767200/1‑151 (MQ=255)
                                                                                                                              gAGTTGGGTGGCGGTACCGCCGCT‑GGGGGCGGGTTGAGCGTGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGACACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGgcg                       <  2:2799801/151‑1 (MQ=255)
                                                                                                                                  tGGGTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAg                   <  1:4581810/151‑1 (MQ=255)
                                                                                                                                     gTGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGGCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCg                <  2:5261374/151‑1 (MQ=255)
                                                                                                                                      tGCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCgg               >  1:4800522/1‑151 (MQ=255)
                                                                                                                                      ggCCGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCgg               <  2:1888080/150‑1 (MQ=255)
                                                                                                                                        ccGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGc             <  1:2103449/151‑1 (MQ=255)
                                                                                                                                         cGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGcc            >  2:3116602/1‑151 (MQ=255)
                                                                                                                                         cGGTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGcc            >  1:3937969/1‑151 (MQ=255)
                                                                                                                                          ggTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGCCa           >  1:3546836/1‑151 (MQ=255)
                                                                                                                                           gTACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGCCAt          <  2:4505989/151‑1 (MQ=255)
                                                                                                                                            tACCGCCGGT‑GGGGGCAGGTTGGGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGCCATg         <  2:1751636/151‑1 (MQ=255)
                                                                                                                                            tACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGCCATg         <  1:703036/151‑1 (MQ=255)
                                                                                                                                            tACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGCCATg         <  1:1588019/151‑1 (MQ=255)
                                                                                                                                            tACCGCCGCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGCCATg         <  1:1319653/151‑1 (MQ=255)
                                                                                                                                              ccgccgCT‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGCCATGGc       <  1:6543402/151‑1 (MQ=255)
                                                                                                                                               cgccgcT‑GGGGGCAGGTTGAGCAGGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGCCATGGCg      <  2:5544132/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
CAGCTCGATCTCTACCCAGCCCAGCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCTGGGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGCCATGGCGGGTG  >  CP009974/3514385‑3514683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑