| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009974 | 4,031,017 | Δ1 bp | intergenic (+159/‑625) | RPPX_17905 → / → RPPX_17915 | 23S rRNA methyltransferase/sulfurtransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009974 | 4,031,017 | 0 | A | . | 89.7% | 111.1 / 16.2 | 40 | intergenic (+159/‑625) | RPPX_17905/RPPX_17915 | 23S rRNA methyltransferase/sulfurtransferase |
| Reads supporting (aligned to +/- strand): ref base A (1/3); new base . (14/21); total (16/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGG > CP009974/4030871‑4031167 | gtTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGccc > 2:4726782/1‑151 (MQ=255) ttctgcttctgcttctgcttctgcttctgcttctgcTTTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGcccc > 1:5640218/1‑151 (MQ=255) tctgcttctgcttctgcttctgcttctgcttctgctTTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCa < 1:1559876/151‑1 (MQ=12) tctgcttctgcttctgcttctgcttctgcttctgctTTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCa < 1:4562159/151‑1 (MQ=16) gctgctgctgcttctgcctctgcttctgcttctgccgTTGCCTCTACGCGGGAGATAGTCCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAGGGGAGGTGACGGGCATGCATGCCCGTCAAGCGCTG‑GGCCCCa < 2:3917900/150‑1 (MQ=11) ttgcttctgcttctgcgtctgcctctgcttctggttTGGCTTGTAAGCGGGCGATGGTCCAGCCTACGCATATTGCGACTTCAGGAGGCCGAGTGGAGGGCTTGCGAAGGGAGGTGACGGCCATGGATGCCCGTCAAGCGCTGAGGCCCCa < 2:4049084/150‑1 (MQ=11) ctgcttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAg > 1:859387/1‑151 (MQ=255) tgcttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAgg > 2:3282437/1‑151 (MQ=12) tgcttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAgg < 1:6248101/151‑1 (MQ=19) tgcttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAgg < 1:6248100/151‑1 (MQ=16) tgcttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAgg < 1:1546503/151‑1 (MQ=12) cttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGa < 1:4530336/150‑1 (MQ=255) cttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGa > 2:4530336/1‑150 (MQ=255) ttctgcttctgcttctgcttctgcttctgcttTTGCTTCTCAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGACCCAGGATg < 2:2529919/151‑1 (MQ=255) ttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATg < 1:3683921/151‑1 (MQ=16) ttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATg > 1:1950784/1‑151 (MQ=255) tctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATgg < 1:4090221/151‑1 (MQ=12) ctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATggg < 1:864851/151‑1 (MQ=16) gcttctgcttctgctgctgcttctgcttTTGCTGCAAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACGTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGc < 2:2755958/151‑1 (MQ=11) cttctgcttcttcttctgcttctgcttTTGATTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGcc < 1:436604/151‑1 (MQ=255) cttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGcc < 2:2130052/151‑1 (MQ=16) cttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGcc > 1:3334901/1‑151 (MQ=12) tctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGcccc < 2:680441/138‑1 (MQ=255) tctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGcccc > 1:680441/1‑138 (MQ=255) tctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtg < 1:4089164/151‑2 (MQ=16) tctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtg > 1:6472640/1‑150 (MQ=16) tctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtg < 1:4602315/151‑2 (MQ=255) ctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGCAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCT‑CGGCCCCAGGATGGGGCCtgc > 2:3709867/1‑149 (MQ=11) ctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGCTGGGGcgtgc > 2:676060/1‑147 (MQ=255) ctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtgc > 2:3686264/1‑149 (MQ=9) tgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtgca < 2:1835848/151‑4 (MQ=255) tgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtgca > 1:435104/1‑148 (MQ=255) gcttctgcttctgcttctgcttTTGGTTCTAAGCGGGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtgcag < 1:5427682/151‑5 (MQ=1) gcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtgcag > 2:2794366/1‑147 (MQ=255) cttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtgcagc < 1:3317236/151‑6 (MQ=255) cttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtgcagc > 2:4436173/1‑146 (MQ=255) ttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtgcagcg < 2:180719/151‑7 (MQ=0) gaggggAATTGATCCACGTAAAGCGCTGAGGCCCGAGGATGGGGCGTTCGGGGCGGTGCTGCCGGGAGCAAGGCCGAAGTGAGGGGACCCCGGAGCGAAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTcgc < 1:437105/148‑1 (MQ=1) gAGGCCCGAGGATGGGGCCGTCGGCGCGGTCCGGCCGGGAGCAAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGCGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTAGCGAAAGACAAAAAGTGACTCGCCGTAAg < 2:1708737/151‑1 (MQ=11) gAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAg > 1:6498490/1‑151 (MQ=11) aGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAgg < 2:4224923/151‑1 (MQ=31) aGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAgg > 1:4224923/1‑151 (MQ=31) | GTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGG > CP009974/4030871‑4031167 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |