Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	4,631,689	Δ1 bp	intergenic (+251/‑579)	emrB → / → RPPX_20645	multidrug resistance protein B/oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	4,631,689	0	G	.	100.0%	129.1 / NA	35	intergenic (+251/‑579)	emrB/RPPX_20645	multidrug resistance protein B/oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (10/25);  total (10/25)

GCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTG  >  CP009974/4631543‑4631712                                                                                                                                                   |                        gcgcAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCg                    >  1:1340265/1‑151 (MQ=255) gcgcAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCg                    <  2:787724/151‑1 (MQ=255)  cgcaGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGt                   >  2:4067199/4‑151 (MQ=255)  cGTAGGGCTGGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGt                   <  2:541925/151‑1 (MQ=255)   gTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGCGCGTAGCGCAGGGGCCGGATGATAGGAgcgagaggg                   >  1:238978/1‑145 (MQ=255)    caGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGttt                 >  1:3635831/2‑151 (MQ=255)     agagCTGGCGAAGGGAGGTGACGGGCATGGATGCACGAAAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCACTGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttt                <  1:1749790/148‑1 (MQ=11)     aGGGCTTGCGCAGGGACGGGACGGGCCTGGATGCCCATCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttt                <  2:4063780/151‑1 (MQ=11)     aGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttt                <  1:4637306/151‑1 (MQ=255)     aGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttt                <  1:673961/151‑1 (MQ=255)     aGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttt                >  1:4533901/1‑151 (MQ=255)     aGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttt                <  1:2527749/151‑1 (MQ=255)      gggCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGttttt               <  1:5259179/151‑1 (MQ=255)      gggCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGttttt               <  1:5368072/151‑1 (MQ=255)       ggCTTGGGAAGGGAGGTGCCGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttttt              <  2:4279162/151‑1 (MQ=255)       ggCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGTCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttttt              <  1:2106339/151‑1 (MQ=255)       ggCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttttt              <  1:1026962/151‑1 (MQ=255)        gCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTg             <  1:905430/151‑1 (MQ=255)         cTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTgg            >  2:4800035/1‑151 (MQ=255)          ttGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGt           >  2:3473759/1‑151 (MQ=255)          ttGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGt           <  2:2034849/151‑1 (MQ=16)          ttGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGCGTTTTGGt           >  2:1382850/1‑151 (MQ=255)          ggacGAAGGGAGGTGGCGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGGATGGGGCCTGCAGAGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGt           <  2:3201622/148‑1 (MQ=11)            gCGAAGGGAGGTGCCGGGCACGGATGCCCGCCAAGCGCTGAGGCCCCATGGATGGGGCCTGCCGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTa         <  2:917834/151‑1 (MQ=11)             ggAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTAc        <  1:4473060/150‑1 (MQ=16)             cGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTAc        <  1:5947040/151‑1 (MQ=255)             cGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTAc        >  1:3075767/1‑151 (MQ=255)              gAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACt       <  2:1650069/151‑1 (MQ=16)               aaGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACtt      <  1:2653372/151‑1 (MQ=12)               aaGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACtt      <  1:1696562/151‑1 (MQ=12)                  ggAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACttttt   >  1:2825174/1‑151 (MQ=9)                  ggAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACttttt   <  2:4476460/151‑1 (MQ=9)                   gAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACTTTTTg  <  2:1473986/151‑1 (MQ=2)                   gAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACTTTTTg  >  2:6166796/1‑151 (MQ=2)                   gAGGTGACGGGCAGGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACTTTTTg  <  1:2370589/151‑1 (MQ=2)                   gAGGTAACGGGCGTGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACTTTTTg  <  1:2088589/151‑1 (MQ=255)                                                                                                                                                   |                        GCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTG  >  CP009974/4631543‑4631712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑