Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 2,825,986 G→A A69V (GCC→GTC)  RPPX_12545 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099742,825,9860GA99.7% 1172.1 / NA 322A69V (GCC→GTC) RPPX_12545hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (155/165);  total (157/165)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 326 total aligned reads displayed.

CCAGAGCTGGCGGGGCGTGGGTGTGTACTTGGCAGGTGGCGGCAGCAGCCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGGCGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCGGGCCCTTGGGCGCAGCAGAAGACAGCGGCATGGCCAGC  >  CP009974/2825838‑2826135
                                                                                                                                                    |                                                                                                                                                     
ccAGAGCTGGCGGGGCGTGGGTGTGTACGTGGCAGGTGGCGGCAGCAGCCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGacg                                                                                                                                                     <  1:1289474/151‑1 (MQ=255)
 cAGAGCTGGCGGGGCGTGGGTGTGTACTTGGCAGGTGGCGGCAGCAGCCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGacgc                                                                                                                                                    >  1:5572927/1‑151 (MQ=255)
   gagCTGGCGGGGCGTGGGTGTGTACTTGGCAGGTGGCGGCAGCAGCCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTg                                                                                                                                                  <  1:5901424/151‑1 (MQ=255)
     gCTGGCGGGGCGTGGGTGTGTACTTGGCAGGTGGCGGCAGCAGCCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTgtc                                                                                                                                                <  2:5555952/151‑1 (MQ=255)
       tGGCGGGGCGTGGGTGTGTACTTGGCAGGTGGCGGCAGCAGCCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTgtcgt                                                                                                                                              <  1:3766135/151‑1 (MQ=255)
       tGGCGGGGCGTGGGTGTGTACTTGGCAGGTGGCGGCAGCAGCCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTgtcgt                                                                                                                                              >  1:4733127/1‑151 (MQ=255)
       tGGCGGGGCGTGGGTGTGTACTTGGCAGGTGGCGGCAGCAGCCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTgtcgt                                                                                                                                              <  2:2752870/151‑1 (MQ=255)
         gCGGGGCGTGGGTGTGTACTTGGCAGGTGGCGGCAGCAGCCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCa                                                                                                                                            <  2:486488/151‑1 (MQ=255)
          cGGGGCGTGGGTGTGTACTTGGCAGGTGGCGGCAGCAGCCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCAc                                                                                                                                           >  2:1182255/1‑151 (MQ=255)
           ggggCGTGGGTGTGTACTTGGCAGGTGGCGGCAGCAGCCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCCCt                                                                                                                                          >  2:3694421/1‑151 (MQ=255)
            gggCGTGGGTGTGTACTTGGCAGGTGGCGGCAGCAGCCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACtt                                                                                                                                         >  1:5371945/1‑151 (MQ=255)
             ggCGTGGGTGTGTACTTGGCAGGTGGCGGCAGCAGCCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACttt                                                                                                                                        >  1:5553495/1‑151 (MQ=255)
             ggCGTGGGTGTGTACTTGGCAGGTGGCGGCAGCAGCCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACttt                                                                                                                                        <  2:2972034/151‑1 (MQ=255)
                  gggTGTGTACTTGGCAGGTGGCGGCAGCAGCCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCggg                                                                                                                                   >  1:4331048/1‑151 (MQ=255)
                    gtgtgtACTTGGCAGGTGGCGGCAGCAGCCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGcc                                                                                                                                 <  2:4018221/151‑1 (MQ=255)
                        gtACTTGGCAGGTGGCGGCAGCAGCCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAgg                                                                                                                             >  1:1393137/1‑151 (MQ=255)
                          aCTTGGCAGGTGGCGGCAGCAGCCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTc                                                                                                                           >  1:6359883/1‑151 (MQ=255)
                          aCTTGGCAGGTGGCGGCAGCAGCCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTc                                                                                                                           <  2:46713/151‑1 (MQ=255)
                             tGGCAGGTGGCGGCAGCAGCCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGAt                                                                                                                        <  2:5253779/151‑1 (MQ=255)
                                cAGGTGGCGGCAGCAGCCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCtt                                                                                                                     <  1:482620/151‑1 (MQ=255)
                                 aGGTGGCGGCAGCAGCCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTg                                                                                                                    <  1:4898573/151‑1 (MQ=255)
                                 aGGTGGCGGCAGCAGCCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTg                                                                                                                    <  1:1127019/151‑1 (MQ=255)
                                  ggTGGCGGCAGCAGCCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCCTGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTgg                                                                                                                   >  2:5942164/1‑151 (MQ=255)
                                   gTGGCGGCAGCAGCCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGc                                                                                                                  <  2:4740235/151‑1 (MQ=255)
                                    gggcggcAGCAGCGCGCTGAGCGGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGcc                                                                                                                 <  2:3150702/150‑1 (MQ=255)
                                     ggcggcAGCAGCCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCa                                                                                                                <  2:874186/151‑1 (MQ=255)
                                       cggcAGCAGCCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAgg                                                                                                              >  1:417443/1‑151 (MQ=255)
                                            gcagcCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTg                                                                                                         <  1:3020149/151‑1 (MQ=255)
                                              agcCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCa                                                                                                                >  2:5005146/1‑142 (MQ=255)
                                               gcCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAAc                                                                                                      <  1:1796766/151‑1 (MQ=255)
                                                  cGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTc                                                                                                   >  1:1976714/1‑151 (MQ=255)
                                                   gCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCg                                                                                                  >  1:5402643/1‑151 (MQ=255)
                                                    cTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGa                                                                                                 >  2:6170623/1‑151 (MQ=255)
                                                     tGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGAc                                                                                                <  1:4030402/151‑1 (MQ=255)
                                                     ggAGCAGTGTCGGCGGGTTGTGCAACTGGGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGAc                                                                                                <  1:3420528/150‑1 (MQ=255)
                                                        gCAGTGGCGGCGAGTTGTGGCACTGTGGCGGGTAGCCGGGCAAAGCCAGCAGCGGCACGACACCGAAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGtt                                                                                             <  1:569155/151‑1 (MQ=255)
                                                            tgtCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGcc                                                                                         <  1:740566/151‑1 (MQ=255)
                                                             gtCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCt                                                                                        <  2:2092892/151‑1 (MQ=255)
                                                              tCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCtt                                                                                       <  2:609598/151‑1 (MQ=255)
                                                              tCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCtt                                                                                       <  2:4449800/151‑1 (MQ=255)
                                                               cggcggGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCttg                                                                                      >  1:2564303/1‑151 (MQ=255)
                                                               cggcggGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGCCGATCTTGGCCAGGGCCTGAACGTCGACGTTGCCCttg                                                                                      >  1:6177871/1‑151 (MQ=255)
                                                                 gcggGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCttgtt                                                                                    >  2:2888582/1‑151 (MQ=255)
                                                                  cggGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTg                                                                                                         <  2:1758399/129‑1 (MQ=255)
                                                                   gggTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTgg                                                                                  >  1:3306020/1‑151 (MQ=255)
                                                                     gTTGTGCAACTGTGGTGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGcc                                                                                <  1:1024263/151‑1 (MQ=255)
                                                                      ttGTGCAAGTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACAGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCa                                                                               <  2:6280019/151‑1 (MQ=255)
                                                                       tgtgCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCAt                                                                              <  1:1682674/151‑1 (MQ=255)
                                                                        gtgCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGGCGTTGGCCTTGTTGGCCATg                                                                             >  1:6486469/1‑151 (MQ=255)
                                                                        gtgCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATg                                                                             <  1:2403224/151‑1 (MQ=255)
                                                                          gCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCt                                                                           >  2:4281096/1‑151 (MQ=255)
                                                                           cAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTg                                                                          >  1:4277714/1‑151 (MQ=255)
                                                                            aaCTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTgg                                                                         <  2:4140179/151‑1 (MQ=255)
                                                                               tgtgGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAAc                                                                      >  1:6028802/1‑151 (MQ=255)
                                                                               tgtgGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAAc                                                                      <  1:228345/151‑1 (MQ=255)
                                                                               ggtgGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTGCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAAc                                                                      <  2:5507081/150‑1 (MQ=255)
                                                                                 tgGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAg                                                                    >  2:2530475/1‑151 (MQ=255)
                                                                                       gTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGcc                                                              >  1:146584/1‑151 (MQ=255)
                                                                                         aGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGcccc                                                            >  1:3855327/1‑151 (MQ=255)
                                                                                         aGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGcccc                                                            <  1:1167797/151‑1 (MQ=255)
                                                                                          gCCGAGCAGGGCCAGCAGCGTCACGGGACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGccccc                                                           <  2:5184002/151‑1 (MQ=255)
                                                                                            cGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCca                                                         <  2:3517510/151‑1 (MQ=255)
                                                                                               gCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAg                                                      >  1:2841489/1‑151 (MQ=255)
                                                                                                   ggCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACg                                                                                               <  1:4969604/106‑1 (MQ=255)
                                                                                                     ccAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTg                                                <  2:5847250/151‑1 (MQ=255)
                                                                                                       agcagcGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGGCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCt                                              >  1:3137349/1‑151 (MQ=255)
                                                                                                        gcagcGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTc                                             <  2:3287894/151‑1 (MQ=255)
                                                                                                         cagcGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCGAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACGGCTCg                                            <  1:4494727/151‑1 (MQ=255)
                                                                                                          agcGGCACGGCACCGGAGCAGCACCATCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTAAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCgg                                           <  2:6073659/151‑1 (MQ=255)
                                                                                                           gcGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGc                                          >  2:2656573/1‑151 (MQ=255)
                                                                                                           gcGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGc                                          <  1:956689/151‑1 (MQ=255)
                                                                                                             ggcacggcacCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCg                                        >  2:5737006/1‑151 (MQ=255)
                                                                                                              gcacggcacCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCgg                                       >  1:4374160/1‑151 (MQ=255)
                                                                                                                acggcacCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCGGGc                                     <  1:5949786/151‑1 (MQ=255)
                                                                                                                    cacCGGAGCAGCACGAGCAGTTCATCATGCCGACGCTGTCGTCGCTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCGGGCCCtt                                 <  2:2428633/151‑1 (MQ=255)
                                                                                                                    cacCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGCCCGGGCCCtt                                 >  2:6387822/1‑151 (MQ=255)
                                                                                                                     acCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCGGGCCCTTg                                >  2:5298659/1‑151 (MQ=255)
                                                                                                                     acCGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCGGGCCCTTg                                >  2:2179144/1‑151 (MQ=255)
                                                                                                                      ccGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCGGGCCCttcg                               >  1:3784413/1‑149 (MQ=255)
                                                                                                                      ccGGAGCAGCACCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAAAGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCGGGCCCTTgg                               <  1:2941539/151‑1 (MQ=255)
                                                                                                                      caggAGCAGCATCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCGGGCCCTTgg                               <  1:2500387/149‑1 (MQ=255)
                                                                                                                           gcagcaCCATCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCTCCCCACAGCAACTGCTCGGCCGGGCCCTTGGGCgca                          <  2:5127551/151‑1 (MQ=255)
                                                                                                                           gcagcaCCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCGGGCCCTTGGGCgca                          >  1:5249939/1‑151 (MQ=255)
                                                                                                                           gcagcaCCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCGGGCCCTTGGGCgca                          <  2:1976714/151‑1 (MQ=255)
                                                                                                                             ggctccAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCGGGCCCTTGGGCgcagc                        <  1:5065253/147‑1 (MQ=255)
                                                                                                                              gcaCCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCGGGCCCTTGGGCgcagca                       >  1:3489579/1‑151 (MQ=255)
                                                                                                                                aCCAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCGGGCCCTTGGGCGCAGCaga                     <  2:3216416/151‑1 (MQ=255)
                                                                                                                                 ccAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCGGGCCCTTGGGCGCAGCagaa                    >  1:2660299/1‑151 (MQ=255)
                                                                                                                                 ccAGCAGTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCGGGCCCTTGGGCGCAGCagaa                    <  2:3055049/151‑1 (MQ=255)
                                                                                                                                   agcagTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCGGGCCCTTGGGCGCAGCagaaga                  <  2:1336093/151‑1 (MQ=255)
                                                                                                                                     cagTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCGGGCCCTTGGGCGCAGCAGAAGACa                <  1:3245043/151‑1 (MQ=255)
                                                                                                                                     cagTTCATCATGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCGGGCCCTTGGGCGCAGCAGAAGACa                <  2:3791730/151‑1 (MQ=255)
                                                                                                                                         tcatcaTGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCGGGCCCTTGGGCGCAGCAGAAGACAGCgg            >  1:4117994/1‑151 (MQ=255)
                                                                                                                                          catcatGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCGGGCCCTTGGGCGCAGCAGAAGACAGCGGc           >  2:1894053/1‑151 (MQ=255)
                                                                                                                                          catcatGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCGGGCCCTTGGGCGCAGCAGAAGACAGCGGc           <  1:2089419/151‑1 (MQ=255)
                                                                                                                                            tcatGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCGGGCCCTTGGGCGCAGCAGAAGACAGCGGCAt         >  1:147917/1‑151 (MQ=255)
                                                                                                                                             catGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCGGGCCCTTGGGCGCAGCAGAAGACAGCGGCATg        <  2:147917/151‑1 (MQ=255)
                                                                                                                                              gtGCCGACGGTGTCGGCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCGTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCGGGCCCTTGGGCGCAGCAGAAGACAGCGGCATgg       <  2:1624040/150‑1 (MQ=255)
                                                                                                                                               tGCCGACGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCGGGCCCTTGGGCGCAGCAGAAGACAGCGGCATGGc      >  2:3395556/1‑151 (MQ=255)
                                                                                                                                                   ggCGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCGGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCGGGCCCTTGGGCGCAGCAGAAGACAGCGGCATGGCcagc  <  2:3427541/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CCAGAGCTGGCGGGGCGTGGGTGTGTACTTGGCAGGTGGCGGCAGCAGCCCGCTGAGCAGTGTCGGCGGGTTGTGCAACTGTGGCGGGTAGCCGGGCAGGGCCAGCAGCGGCACGGCACCGGAGCAGCACCAGCAGTTCATCATGCCGGCGCTGTCGTCACTTTGCGGGCCTAGGTCGATCTTGGCCAGGGCCTGAACGTCGACGTTGGCCTTGTTGGCCATGCTGGAACAGAAAGCCCCCCACAGCAACTGCTCGGCCGGGCCCTTGGGCGCAGCAGAAGACAGCGGCATGGCCAGC  >  CP009974/2825838‑2826135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑