Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 2,982,248 T→G intergenic (‑327/+619) RPPX_13295 ← / ← RPPX_13300 integrase/integrase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099742,982,2480TG99.7% 1076.0 / ‑6.7 305intergenic (‑327/+619)RPPX_13295/RPPX_13300integrase/integrase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (158/146);  total (158/147)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 307 total aligned reads displayed.

CCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCTTGGTCTGCGGCACCATCATCTACATCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGCACAGGTTGGCGCAATATTC  >  CP009974/2982101‑2982395
                                                                                                                                                   |                                                                                                                                                   
  ggTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTg                                                                                                                                                >  1:1019381/1‑151 (MQ=255)
    tGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGtt                                                                                                                                              <  2:4915504/151‑1 (MQ=255)
    tGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGACATGGGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGCCTGCGGCACCATCATCTACCGCGCTGtt                                                                                                                                              >  2:4218100/1‑151 (MQ=14)
     gggCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTg                                                                                                                                             >  1:528090/1‑151 (MQ=255)
     gggCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTg                                                                                                                                             >  1:298453/1‑151 (MQ=255)
        cGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTg                                                                                                                                          >  1:4464811/1‑151 (MQ=255)
          ggTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGt                                                                                                                                        <  2:3574916/151‑1 (MQ=255)
              gAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACa                                                                                                                                    <  1:2651169/151‑1 (MQ=255)
              gAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGGGACa                                                                                                                                    >  2:1361276/1‑151 (MQ=255)
               aGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGCTGACCGGGGCATGCCTCACTGTCAGTCAGGTGCAGTCCATTCGGTGGTCTGCGGCACCCTCATCTCGAGCGCTGTTGCTGTTGAcct                                                                                                                                   >  2:3968832/1‑149 (MQ=14)
                  cTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCgg                                                                                                                                <  1:3378080/151‑1 (MQ=255)
                   tGCATTGGCTGACGAGACCAGTGCCGCGAGAACCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGc                                                                                                                               >  1:4260303/1‑151 (MQ=255)
                    gCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATc                                                                                                                                  >  2:4617635/1‑147 (MQ=255)
                      aTTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGtt                                                                                                                            >  1:1628460/1‑151 (MQ=255)
                       ttGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGttt                                                                                                                           >  2:2445489/1‑151 (MQ=255)
                            tGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCAc                                                                                                                      >  1:3147255/1‑151 (MQ=255)
                            tGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCAc                                                                                                                      <  2:3425130/151‑1 (MQ=255)
                                gagaCCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCg                                                                                                                  >  2:1835711/1‑151 (MQ=255)
                                  gaCCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGAt                                                                                                                >  2:447316/1‑151 (MQ=255)
                                  gaCCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGGTGCTGGTGACAGCGGCGTCTGTCACGCCGAt                                                                                                                >  2:4294951/1‑151 (MQ=14)
                                      aGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTc                                                                                                            >  2:1020356/1‑151 (MQ=255)
                                      aGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTc                                                                                                            >  1:6364308/1‑151 (MQ=255)
                                         gCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACg                                                                                                         <  2:5716259/151‑1 (MQ=255)
                                            gcgAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAgg                                                                                                      >  1:435209/1‑151 (MQ=255)
                                            gcgAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAgg                                                                                                      <  1:450384/151‑1 (MQ=255)
                                              gagaTCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAgggg                                                                                                    >  1:5906622/1‑151 (MQ=255)
                                              gagaTCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAgggg                                                                                                    >  1:901257/1‑151 (MQ=255)
                                              gagaTCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACCAgggg                                                                                                    >  2:4723551/1‑151 (MQ=255)
                                                gaTCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAgggggn                                                                                                  <  1:2456484/151‑2 (MQ=255)
                                                gaTCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGa                                                                                                  <  1:1734036/151‑1 (MQ=255)
                                                 aTCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGaa                                                                                                 >  1:4903043/1‑151 (MQ=255)
                                                  tCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAAc                                                                                                >  2:6004565/1‑151 (MQ=255)
                                                     tGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAAcgcc                                                                                             >  1:755580/1‑151 (MQ=255)
                                                        gcgaTGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCa                                                                                          <  2:1217619/148‑1 (MQ=255)
                                                         ccATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCaa                                                                                         <  2:5229041/151‑1 (MQ=255)
                                                              tgCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGATGGGGAACGCCGCAAAGCCa                                                                                    >  1:4292794/1‑151 (MQ=255)
                                                                 aaGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCAtgt                                                                                 >  1:2294665/1‑151 (MQ=255)
                                                                 aaGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCAtgt                                                                                 <  2:6093751/151‑1 (MQ=255)
                                                                   gcagcaATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCCATTGTCACGCGGGGGCACGCCGCAAAGCCATGTGc                                                                               >  2:2180091/1‑151 (MQ=255)
                                                                    cagcaATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCg                                                                              >  1:3156016/1‑151 (MQ=255)
                                                                        aaTGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATcc                                                                          >  2:6450042/1‑150 (MQ=255)
                                                                           gCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCTATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCACAGCCATGTGCGATCAggg                                                                       >  1:5065947/1‑151 (MQ=255)
                                                                           gCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAggg                                                                       <  2:4570263/151‑1 (MQ=255)
                                                                            cGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCAGCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGc                                                                      <  2:6217677/151‑1 (MQ=255)
                                                                             gATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCt                                                                     >  2:3095134/1‑151 (MQ=255)
                                                                             gATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCt                                                                     <  2:5787211/151‑1 (MQ=255)
                                                                              aTGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAAcgccgc                                                                                           >  2:5446692/1‑128 (MQ=255)
                                                                              aTGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAAcgccgc                                                                                           <  1:5446692/128‑1 (MQ=255)
                                                                               tGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTgc                                                                   <  1:4200339/151‑1 (MQ=255)
                                                                                caCCGGCGCATGCCTGACTCTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTGGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTgca                                                                  <  2:4382515/150‑1 (MQ=14)
                                                                                 aCCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTgcag                                                                 <  2:6212232/151‑1 (MQ=255)
                                                                                  ccGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTgcagc                                                                >  2:2939046/1‑151 (MQ=255)
                                                                                    ggggCATGCCTGGCTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGcagcag                                                              <  2:249533/151‑1 (MQ=255)
                                                                                      ggCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTg                                                            <  1:366668/151‑1 (MQ=255)
                                                                                        cATGCTTGACTGTCAGGCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGt                                                          <  2:5139782/151‑1 (MQ=255)
                                                                                        cATGCCTGACTGTCGGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGt                                                          <  2:875836/151‑1 (MQ=255)
                                                                                        cATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGt                                                          >  2:632891/1‑151 (MQ=255)
                                                                                         aTGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTa                                                         >  2:3478837/1‑151 (MQ=255)
                                                                                           gCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGa                                                       >  1:353567/1‑151 (MQ=255)
                                                                                             cTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAg                                                     <  1:6015390/151‑1 (MQ=255)
                                                                                             cTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAg                                                     <  1:1190633/151‑1 (MQ=255)
                                                                                                aCTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGc                                                  <  1:4617296/151‑1 (MQ=255)
                                                                                                 cTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGcc                                                 <  1:5339617/151‑1 (MQ=255)
                                                                                                   gtcagtcaGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGCTCAGGGCTGCAGCAGTGGTAGAAGTGCCCg                                               >  2:4773661/1‑151 (MQ=255)
                                                                                                   gtcagtcaGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCg                                               <  1:651625/151‑1 (MQ=255)
                                                                                                        tcagGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTctct                                          >  1:4691396/1‑151 (MQ=255)
                                                                                                        ccagGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTctct                                          <  1:1433014/150‑1 (MQ=255)
                                                                                                         cagGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCtt                                         <  2:1488420/151‑1 (MQ=255)
                                                                                                         cagGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCtt                                         >  2:4062493/1‑151 (MQ=255)
                                                                                                          agGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCttt                                        <  2:2640233/151‑1 (MQ=255)
                                                                                                            gTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTcc                                      <  1:6282597/151‑1 (MQ=255)
                                                                                                            gTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTcc                                      >  1:1095851/1‑151 (MQ=255)
                                                                                                             tGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCa                                     >  2:906620/1‑151 (MQ=255)
                                                                                                               cAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCaag                                   >  1:2797764/1‑151 (MQ=255)
                                                                                                                  tCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCaagaga                                <  2:5906622/151‑1 (MQ=255)
                                                                                                                   ccATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCaagagaa                               <  1:254484/151‑1 (MQ=255)
                                                                                                                   ccATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCaagagaa                               >  2:1392095/1‑151 (MQ=255)
                                                                                                                     aTTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCaagagaaag                             <  2:3721344/151‑1 (MQ=255)
                                                                                                                       tccgtGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCaagagaaagag                           <  2:2730364/147‑1 (MQ=255)
                                                                                                                          gtGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACg                        <  1:6177068/150‑1 (MQ=255)
                                                                                                                          gtGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACg                        <  1:3886387/150‑1 (MQ=255)
                                                                                                                          gtGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACg                        >  2:1088827/2‑151 (MQ=255)
                                                                                                                          gtGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACg                        <  2:740210/150‑1 (MQ=255)
                                                                                                                          gtGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACg                        <  1:1328107/150‑1 (MQ=255)
                                                                                                                           tGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACgg                       <  1:1578488/151‑1 (MQ=255)
                                                                                                                           gggTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACgg                       <  2:657536/150‑1 (MQ=255)
                                                                                                                             gTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGCGACGGGc                     >  2:1702912/1‑151 (MQ=255)
                                                                                                                              tCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGca                    >  2:3014472/1‑151 (MQ=255)
                                                                                                                              tCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGca                    >  1:197461/1‑151 (MQ=255)
                                                                                                                                  cGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGCACAgg                <  1:5960568/151‑1 (MQ=255)
                                                                                                                                  cGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGCACAgg                <  1:4588288/151‑1 (MQ=255)
                                                                                                                                     cACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGCACAGGTTg             >  1:2514098/1‑151 (MQ=255)
                                                                                                                                     cACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGCACAGGTTg             >  1:2180817/1‑151 (MQ=255)
                                                                                                                                       ccATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGCACAGGTTGgc           <  2:2341782/151‑1 (MQ=255)
                                                                                                                                        catcatCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGCACAGGTTGgcg          >  1:4034994/1‑151 (MQ=255)
                                                                                                                                             tcTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGCACAGGTTGGCGCAata     >  2:3245915/1‑151 (MQ=255)
                                                                                                                                             tcTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGCACAGGTTGGCGCAata     >  2:1549957/1‑151 (MQ=255)
                                                                                                                                               tACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGcaca                  >  1:5776964/1‑136 (MQ=255)
                                                                                                                                               tACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGCACAGGTTGGCGCAATAtt   <  2:4790919/151‑1 (MQ=255)
                                                                                                                                               tACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGCACAGGTTGGCGCAATAtt   <  2:1189416/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCTTGGTCTGCGGCACCATCATCTACATCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGCACAGGTTGGCGCAATATTC  >  CP009974/2982101‑2982395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑