Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 3,079,418 C→T intergenic (+46/+85) RPPX_13695 → / ← RPPX_13700 membrane protein/LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099743,079,4180CT99.7% 1195.9 / NA 326intergenic (+46/+85)RPPX_13695/RPPX_13700membrane protein/LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (167/158);  minor base G (0/1);  total (167/159)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 334 total aligned reads displayed.

TCAGAGCCGTGGCCTGGGCAGCAGCCAGCCGGTGGCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAACGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTCGCGGGTTTGCCCCATCAACAATGGCGCATTCGCCTCGGT  >  CP009974/3079270‑3079565
                                                                                                                                                    |                                                                                                                                                   
tCAGAGCCGTGGCCTGGGCAGCAGCCAGCCGGTGGCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAAtgc                                                                                                                                                   <  2:2266415/151‑1 (MQ=255)
  agagCCGTGGCCTGGGCAGCAGCCAGCCGGTGGCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCgg                                                                                                                                                 <  2:2066276/151‑1 (MQ=255)
    agCCGTGGCCTGGGCAGCAGCCAGCCGGTGGCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGcc                                                                                                                                               >  1:6041286/1‑151 (MQ=255)
      ccGTGGCCTGGGCAGCAGCCAGCCGGTGGCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTg                                                                                                                                             >  1:3658461/1‑151 (MQ=255)
          ggCCTGGGCAGCAGCCAGCCGGTGGCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCAc                                                                                                                                         <  1:1570688/151‑1 (MQ=255)
          ggCCTGGGCAGCAGCCAGCCGGTGGCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCAc                                                                                                                                         >  1:3097926/1‑151 (MQ=255)
           gCCTGGGCAGCAGCCAGCCGGTGGCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCAcc                                                                                                                                        >  1:2045166/1‑151 (MQ=255)
           gCCTGGGCAGCAGCCAGCCGGTGGCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCAcc                                                                                                                                        >  1:2118032/1‑151 (MQ=255)
                ggCAGCAGCCAGCCGGTGGCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCtt                                                                                                                                   <  1:4462884/151‑1 (MQ=255)
                   agcagcCAGCCGGTGGCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGc                                                                                                                                <  2:6358677/151‑1 (MQ=255)
                   agcagcCAGCCGGTGGCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGCGTCCACCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCCGCTTCGc                                                                                                                                >  2:6110163/1‑151 (MQ=255)
                     cagccagcCGGTGGCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCg                                                                                                                              >  2:3534133/1‑151 (MQ=255)
                       gccagccGGTGGCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGgt                                                                                                                            <  1:3019644/151‑1 (MQ=255)
                       gccagccGGTGGCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGgt                                                                                                                            <  1:1616929/151‑1 (MQ=255)
                       gccagccGGTGGCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGgt                                                                                                                            <  1:557620/151‑1 (MQ=255)
                        ccagccGGTGGCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGCGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGgtg                                                                                                                           >  2:2205505/1‑151 (MQ=255)
                         cagccGGTGGCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGgtgt                                                                                                                          <  1:4608360/151‑1 (MQ=255)
                         cagccGGTGGCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCGACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGgtgt                                                                                                                          <  2:609189/151‑1 (MQ=255)
                         cagccGGTGGCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGCGGTTGCGGCCTACTGCTAGTTGCCGCTCGGGCTGTCACATATCCGCTGTCGGCGCTGGCAATGCGGCCTGGCGCCGTCTTGGCCGgtgt                                                                                                                          >  2:3872522/1‑151 (MQ=255)
                          agccGGTGGCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAAtgc                                                                                                                                                   <  2:2164961/125‑1 (MQ=255)
                          agccGGTGGCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGCGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCACTGCGGCCTGGCCCCGGCTTCGCCGGTGtt                                                                                                                         >  2:3138893/1‑151 (MQ=255)
                             cGGTGGCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTcgc                                                                                                                      <  1:1020200/151‑1 (MQ=255)
                             cGGTGGCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTcgc                                                                                                                      <  1:3332334/151‑1 (MQ=255)
                              ggTGGAGGACAACAGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTcgcg                                                                                                                     <  1:2007498/151‑1 (MQ=255)
                               gTGGCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCgg                                                                                                                    >  1:613321/1‑151 (MQ=255)
                                tGGCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCggg                                                                                                                   >  2:5800735/1‑151 (MQ=255)
                                tGGCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCggg                                                                                                                   >  1:2480206/1‑151 (MQ=255)
                                  gCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCa                                                                                                                 <  1:5401074/151‑1 (MQ=255)
                                   cGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCaa                                                                                                                >  2:4182493/1‑151 (MQ=255)
                                       cAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGccc                                                                                                            >  2:5238491/1‑151 (MQ=255)
                                       cAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGccc                                                                                                            <  1:796729/151‑1 (MQ=255)
                                                      aGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCt                                                                                             >  2:769302/1‑151 (MQ=255)
                                                        ggCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCcgt                                                                                           >  1:6085288/1‑151 (MQ=255)
                                                          cGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCACTGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTgtgt                                                                                         >  2:3049802/1‑151 (MQ=255)
                                                            tACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTg                                                                                       <  1:1318880/151‑1 (MQ=255)
                                                             aCCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGc                                                                                      >  1:4907042/1‑151 (MQ=255)
                                                                 ggaACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTc                                                                                  <  2:4356827/150‑1 (MQ=255)
                                                                 agaACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCCCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGCGCTGCCTTc                                                                                  >  2:4846734/1‑151 (MQ=255)
                                                                  gaACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCg                                                                                 >  2:3745120/1‑151 (MQ=255)
                                                                   aaCCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCgg                                                                                >  2:3216684/1‑151 (MQ=255)
                                                                     ccGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTGGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGaa                                                                              <  2:1556767/151‑1 (MQ=255)
                                                                      cGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGaaa                                                                             >  1:5159422/1‑151 (MQ=255)
                                                                       gTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAAt                                                                            <  2:4236684/151‑1 (MQ=255)
                                                                       gTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGCGGTGCCTTCCGCAAt                                                                            >  2:5054960/1‑151 (MQ=255)
                                                                         cGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATgc                                                                          >  1:3944912/1‑151 (MQ=255)
                                                                          gggTGTCCATCGTGGTTGGGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGGAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATgcg                                                                         <  2:1144336/151‑1 (MQ=255)
                                                                            gtgtCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATgcgcc                                                                       >  2:5851310/1‑150 (MQ=255)
                                                                             tgtCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCGGTCACATATCCCCGGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAg                                                                      <  2:4944356/151‑1 (MQ=255)
                                                                             tggccATCGTGGTAGGGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAg                                                                      <  2:5548265/148‑1 (MQ=255)
                                                                                ccATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATgcgcgagcc                                                                   >  1:4314261/1‑146 (MQ=255)
                                                                                ccATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGcc                                                                   <  1:5914347/151‑1 (MQ=255)
                                                                                  aTCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCa                                                                 >  1:4774943/1‑151 (MQ=255)
                                                                                     gTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCa                                                              <  1:1120622/151‑1 (MQ=255)
                                                                                     gTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTGCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCa                                                              <  2:5882476/151‑1 (MQ=255)
                                                                                     gTGGTTGCGTCCTACTGAGCGTTGCCGCTCGGGCTGTCACATAGCCCCTGTAGGCGCTGGCAATGCGGCCTGGCCCCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACCGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCa                                                              <  2:1633735/151‑1 (MQ=255)
                                                                                      tGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCaa                                                             >  1:3562412/1‑150 (MQ=255)
                                                                                            cGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATCTCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTgg                                                       <  2:3934953/151‑1 (MQ=255)
                                                                                            cGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTgg                                                       <  1:5967941/151‑1 (MQ=255)
                                                                                             gTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTggg                                                      >  1:4731986/1‑151 (MQ=255)
                                                                                             gTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTggg                                                      >  1:5769278/1‑151 (MQ=255)
                                                                                              tCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGc                                                     <  1:4174828/151‑1 (MQ=255)
                                                                                              tCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGc                                                     <  2:5717477/151‑1 (MQ=255)
                                                                                                cTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCaa                                                   >  2:817884/1‑151 (MQ=255)
                                                                                                cTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCaa                                                   >  2:2657903/1‑151 (MQ=255)
                                                                                                     gATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTCGGCGCTGGCAATCCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCCGTGTTGCCTTCGGCAATCCGCAGGCCCATCCGTCCTTGGCAAAgcgc                                              >  2:6173550/1‑151 (MQ=38)
                                                                                                      aTCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCa                                             >  2:2346343/1‑151 (MQ=255)
                                                                                                       tCGTTGCCGCTCGGGCTGTCACATTTCCTCTGTAGGTGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGTAAAGTGCAt                                            >  1:2653338/1‑151 (MQ=38)
                                                                                                       tCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCAt                                            >  1:1498143/1‑151 (MQ=255)
                                                                                                          ttGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCACTGCCGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCAtctc                                         <  2:5023316/151‑1 (MQ=255)
                                                                                                          ttGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGACATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCAtctc                                         >  2:4571875/1‑151 (MQ=255)
                                                                                                          ttGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCAtctc                                         <  2:3337002/151‑1 (MQ=255)
                                                                                                          ttGCCGCGCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCAtctc                                         <  2:3035591/151‑1 (MQ=255)
                                                                                                             ccGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGTCAAGCCCGCTCCTACAGGGGCTGTTTTGCCTTCGTAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTtgcg                                      >  2:316040/1‑151 (MQ=255)
                                                                                                                 tCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTCGCGGGtt                                  >  1:3828113/1‑151 (MQ=255)
                                                                                                                  cGGGCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTCGCGGGttt                                 <  2:4774943/151‑1 (MQ=255)
                                                                                                                  cGGGCTGTCACAGATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTCGCGGGttt                                 <  2:2893369/151‑1 (MQ=255)
                                                                                                                   gggCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTCGCGGGTTTg                                <  2:3289574/151‑1 (MQ=255)
                                                                                                                    ggCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTCGCGGGTTTGc                               >  2:1773704/1‑151 (MQ=255)
                                                                                                                     gCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTCGCGGGTTTGcc                              >  1:509826/1‑151 (MQ=255)
                                                                                                                     gCTGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTCGCGGGTTTGcc                              >  1:5429076/1‑151 (MQ=255)
                                                                                                                       tGTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTCGCGGGTTTGcccc                            >  2:1811556/1‑151 (MQ=255)
                                                                                                                       ggTCACATATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCCGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTCGCGGGTTTGcccc                            <  2:4095289/150‑1 (MQ=255)
                                                                                                                          cacaTATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTCGCGGGTTTGCCCCATc                         <  1:6439937/151‑1 (MQ=255)
                                                                                                                           gcaTAGCCCCTGTAGGCGCGGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCGAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTCGCGGGTTTGCCCCATca                        <  2:4533679/150‑1 (MQ=255)
                                                                                                                           acaTATCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTCGCGGGTTTGCCCCATca                        <  1:2750521/151‑1 (MQ=255)
                                                                                                                                tCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTCGCGGGTTTGCCCCATCAACAAt                   <  2:5159422/151‑1 (MQ=255)
                                                                                                                                tCCCCTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTCGCGGGTTTGCCCCATCAACAAt                   >  1:339971/1‑151 (MQ=255)
                                                                                                                                  cccTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTCGCGGGTTTGCCCCATCAACAATgg                 <  2:2819533/151‑1 (MQ=255)
                                                                                                                                   ccTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTCGCGGGTTTGCCCCATCAACAATGgc                <  1:6227881/151‑1 (MQ=255)
                                                                                                                                   ccTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTCGCGGGTTTGCCCCATCAACAATGgc                >  2:1045113/1‑151 (MQ=255)
                                                                                                                                   ccTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTCGCGGGTTTGCCCCATCAACAATGgc                <  2:2520071/151‑1 (MQ=255)
                                                                                                                                    cTGTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTCGCGGGTTTGCCCCATCAACAATGgcg               <  1:5199371/151‑1 (MQ=255)
                                                                                                                                      gTAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTCGCGGGTTTGCCCCATCAACAATGGCGCa             >  1:933592/1‑151 (MQ=255)
                                                                                                                                       tAGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTCGCGGGTTTGCCCCATCAACAATGGCGCAt            >  1:2904961/1‑151 (MQ=255)
                                                                                                                                        aGGCGCTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTCGCGGGTTTGCCCCATCAACAATGGCGCAtt           <  1:628500/151‑1 (MQ=255)
                                                                                                                                            gcTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTCGCGGGTTTGCCCCATCAACAATGGCGCATTCGcc       <  1:3216684/151‑1 (MQ=255)
                                                                                                                                            gcTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTCGCGGGTTTGCCCCATCAACAATGGCGCATTCGcc       <  1:6537366/151‑1 (MQ=255)
                                                                                                                                             cTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTCCAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCGCATCAGCCCTGGGCAAAGCGCAGCTCGCGGGTTTGCCCCACCAACAAGGGCGCATGCGccc      >  2:6439930/1‑150 (MQ=38)
                                                                                                                                             cTGGCAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTCGCGGGTTTGCCCCATCAACAATGGCGCATTCGCCt      <  2:6316157/151‑1 (MQ=255)
                                                                                                                                                 cAATGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTCGCGGGTTTGCCCCATCAACAATGGCGCATTCGCCTCGGt  <  1:4839673/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCAGAGCCGTGGCCTGGGCAGCAGCCAGCCGGTGGCGGACAACCGCACCAGTGCAGGGCGTACCGAGAACCGTCGGGTGTCCATCGTGGTTGCGTCCTACTGATCGTTGCCGCTCGGGCTGTCACATATCCCCTGTAGGCGCTGGCAACGCGGCCTGGCACCGGCTTCGCCGGTGTTCGCGGGCAAGCCCGCTCCTACAGGGGCTGTGTTGCCTTCGGAAATGCGCAGGCCCATCAGTCCTGGGCAAAGCGCATCTCGCGGGTTTGCCCCATCAACAATGGCGCATTCGCCTCGGT  >  CP009974/3079270‑3079565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑