Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 3,297,192 T→G T53P (ACC→CCC)  RPPX_14685 ← AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099743,297,1920TG99.7% 1051.2 / NA 293T53P (ACC→CCC) RPPX_14685AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (134/158);  total (134/159)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 315 total aligned reads displayed.

TCGGGGCTGGCATCCAGCCTCACGGCACTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑A‑‑GGGT‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCTCCAGGCGCTGCAGTTCCAGGGTCGGTTCGGTGGACGTGGC  >  CP009974/3297044‑3297339
                                                                                                                                                       |                                                                                                                                                    
 cGGGGCTGGCATCCAGCCTCACGGCACTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑A‑‑gggg‑ggg                                                                                                                                                  >  2:3096687/1‑151 (MQ=255)
    ggCTGGCATCCAGCCTCACGGCACTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑A‑‑gggg‑ggggta                                                                                                                                               <  1:5556244/151‑4 (MQ=255)
    ggCTGGCATCCAGCCTCACGGCACTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑A‑‑gggg‑ggggta                                                                                                                                               <  1:3740937/151‑4 (MQ=255)
    ggCTGGCATCCAGCCTCACGGCACTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑A‑‑gggg‑ggggta                                                                                                                                               <  2:544926/151‑4 (MQ=255)
    ggCTGGCATCCAGCCTCACGGCACTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑A‑‑gggg‑ggggta                                                                                                                                               <  2:5690062/151‑4 (MQ=255)
     gCTGGCATCCAGCCTCACGGCACTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑A‑‑gggg‑ggggtac                                                                                                                                              >  1:3444988/1‑147 (MQ=255)
      cTGGCATCCAGCCTCACGGCACTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑A‑‑gggg‑ggggtaca                                                                                                                                             <  1:905469/151‑6 (MQ=255)
      cTGGCATCCAGCCTCACGGCACTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑A‑‑gggg‑ggggtaca                                                                                                                                             <  2:2219475/151‑6 (MQ=255)
        ggCATCCAGCCTCACGGCACTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGa                                                                                                                                            <  2:2118242/151‑1 (MQ=255)
        ggCATCCAGCCTCACGGCACTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGa                                                                                                                                            <  2:4398726/151‑1 (MQ=255)
          cATCCAGCCTCACGGCACTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAAc                                                                                                                                          <  2:5938112/151‑1 (MQ=255)
           aTCCAGCCTCACGGCACTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACg                                                                                                                                         <  1:6034391/151‑1 (MQ=255)
            tCCAGCCTCACGGCACTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑A‑‑GGGG‑GGGTACAGAACGca                                                                                                                                       <  2:5346040/151‑1 (MQ=255)
                 ccTCACGGCACTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACAtt                                                                                                                                   <  2:4802017/151‑1 (MQ=255)
                  cTCACGGCACTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑A‑‑GGGGGGGGTACAGAACGCACAttt                                                                                                                                  >  1:1814500/1‑151 (MQ=255)
                   tCACGGCACTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACAtttt                                                                                                                                 <  1:5904716/151‑1 (MQ=255)
                     aCGGCACTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCg                                                                                                                               >  1:4360750/1‑151 (MQ=255)
                      cGGCACTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCgg                                                                                                                              <  2:2466460/151‑1 (MQ=255)
                      cGGCACTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCgg                                                                                                                              <  1:394440/151‑1 (MQ=255)
                       ggCACTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGgt                                                                                                                             <  1:6407261/151‑1 (MQ=255)
                        gcaacgAGTGGCAAGGTCACCGAAACGACCTTGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAGCAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGGACGCACATTTTCGgtg                                                                                                                            <  2:1442071/146‑1 (MQ=255)
                         cACTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGgtgt                                                                                                                           <  2:3632138/151‑1 (MQ=255)
                         cACTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGgtgt                                                                                                                           >  2:1211161/1‑151 (MQ=255)
                           cTGAGTGGCAAGGTCACCGCAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTaa                                                                                                                         <  2:6453254/151‑1 (MQ=255)
                           cTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCTTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTaa                                                                                                                         <  1:221901/151‑1 (MQ=255)
                            tGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAAc                                                                                                                        <  1:4545177/151‑1 (MQ=255)
                            tGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAAc                                                                                                                        >  1:2477732/1‑151 (MQ=255)
                            tGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAAc                                                                                                                        <  1:2088966/151‑1 (MQ=255)
                                tGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCAc                                                                                                                    <  2:2912015/151‑1 (MQ=255)
                                   cAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAg                                                                                                                 <  2:2863438/151‑1 (MQ=255)
                                    aaGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAgg                                                                                                                <  2:323696/151‑1 (MQ=255)
                                        tCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACa                                                                                                            >  1:3819170/1‑151 (MQ=255)
                                         cACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTCCGGTGTAACGCACCAGGTACAg                                                                                                           >  2:5144099/1‑151 (MQ=255)
                                             gAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCa                                                                                                       >  1:2953941/1‑151 (MQ=255)
                                               aaCGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATc                                                                                                     <  2:1814500/151‑1 (MQ=255)
                                                aCGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCg                                                                                                    <  1:5357187/151‑1 (MQ=255)
                                                   aCCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATg                                                                                                 <  1:1258238/151‑1 (MQ=255)
                                                      aTGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AGGGGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGc                                                                                               >  2:5881573/1‑151 (MQ=255)
                                                          aaggCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAGCAGGCTTGTGCGGCCTTGGGCAAGGTTGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATg                                                                                          <  2:5654704/148‑1 (MQ=255)
                                                          aaTGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATg                                                                                          >  1:1103776/1‑151 (MQ=255)
                                                          aaTGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATg                                                                                          >  1:1132989/1‑151 (MQ=255)
                                                              cAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTgg                                                                                      >  1:1984887/1‑151 (MQ=255)
                                                               aGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTggg                                                                                     <  1:2698352/151‑1 (MQ=255)
                                                                 tGGGTCGGACGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGcc                                                                                   <  2:6081868/151‑1 (MQ=255)
                                                                  gggTGGTAGGCGTACTGGTCGTCACCCGGGAACGGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCa                                                                                  <  2:5233907/151‑1 (MQ=255)
                                                                  gggTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCa                                                                                  >  1:4981225/1‑151 (MQ=255)
                                                                   gggcGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCaa                                                                                 <  1:4061537/148‑1 (MQ=255)
                                                                    gTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCaaa                                                                                >  1:74335/1‑151 (MQ=255)
                                                                    gTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCaaa                                                                                >  1:3525943/1‑151 (MQ=255)
                                                                       gTAGGCGTACTGCTCGGCACCCAGGAACAGGGTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AGGGGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAgg                                                                              <  1:5893051/151‑1 (MQ=255)
                                                                            cGTACTGCTCGTCAGCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCGGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGc                                                                        <  2:6535979/151‑1 (MQ=255)
                                                                            cGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGc                                                                        <  1:4496604/151‑1 (MQ=255)
                                                                             gTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGcc                                                                       <  1:845758/151‑1 (MQ=255)
                                                                             gTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGcc                                                                       <  2:1899863/151‑1 (MQ=255)
                                                                                 tGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCa                                                                   >  1:4935808/1‑151 (MQ=255)
                                                                                   cTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATg                                                                 >  1:2315421/1‑151 (MQ=255)
                                                                                     ggTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATCCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGc                                                               <  2:5018256/150‑1 (MQ=255)
                                                                                     cGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGc                                                               >  1:414523/1‑151 (MQ=255)
                                                                                      gTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCg                                                              <  1:4628855/151‑1 (MQ=255)
                                                                                      gTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCg                                                              <  1:5596105/151‑1 (MQ=255)
                                                                                       tCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCgg                                                             <  1:4109885/151‑1 (MQ=255)
                                                                                          cgcAGGAACAGGCTTTTGCTGCCTTGGGCAAGAATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTg                                                          <  1:3666499/149‑1 (MQ=255)
                                                                                          cccAGGAACAGGCTTGTGCTGCCTTGGGCAAGGAGCCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTg                                                          <  2:3917036/151‑1 (MQ=255)
                                                                                            cAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGAt                                                        <  1:4322446/151‑1 (MQ=255)
                                                                                            cAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGAt                                                        >  1:2576700/1‑151 (MQ=255)
                                                                                            cAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGAt                                                        >  2:5386558/1‑151 (MQ=255)
                                                                                             aGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATc                                                       <  2:1092263/151‑1 (MQ=255)
                                                                                              ggAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCa                                                      <  2:1280769/151‑1 (MQ=255)
                                                                                               gAACAGGCTTTTGCTGCGTTGGGCAAGGATGCGCAGGGGAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAg                                                     <  2:1698566/151‑1 (MQ=255)
                                                                                               gAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAg                                                     <  1:1461749/151‑1 (MQ=255)
                                                                                               gAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAg                                                     <  1:3394083/151‑1 (MQ=255)
                                                                                                aaCAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAGc                                                    >  1:262928/1‑151 (MQ=255)
                                                                                                 aCAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCt                                                   >  2:1389778/1‑151 (MQ=255)
                                                                                                    ggCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCgg                                                <  1:5773443/151‑1 (MQ=255)
                                                                                                      ggtttGGTGCCTTGGGCAAGGATGCACAGGGCAGGCTGGGCC‑A‑‑GGGGGGGGTACAGAACGCAGATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAGGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGcc                                              <  2:2632527/149‑1 (MQ=255)
                                                                                                       ttttGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCa                                             <  1:468429/151‑1 (MQ=255)
                                                                                                         ttGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑A‑‑GGGGGGGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGc                                           <  1:5209218/151‑1 (MQ=255)
                                                                                                          tgctgcCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGAACATTTTCGGTGTAACGCACCAGGTACAGGTCCTCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCt                                          <  1:1045013/151‑1 (MQ=255)
                                                                                                            ctgcCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCTcc                                        >  2:2243633/1‑151 (MQ=255)
                                                                                                             tgcCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCTCCa                                       <  1:3177531/151‑1 (MQ=255)
                                                                                                               ccTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCTCCAgg                                     <  2:366651/151‑1 (MQ=255)
                                                                                                                 ttGGGCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACAAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCTCCAGgcg                                   >  1:1649238/1‑151 (MQ=255)
                                                                                                                     gCAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCTCCAGGCGCTGc                               >  2:6473001/1‑151 (MQ=255)
                                                                                                                      cAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAg                                                                               >  2:1485465/1‑102 (MQ=255)
                                                                                                                      cAAGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCTCCAGGCGCTGCa                              >  2:159597/1‑151 (MQ=255)
                                                                                                                       aaGGATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCTCCAGGCGCTGCAg                             >  1:6165030/1‑151 (MQ=255)
                                                                                                                          gATGCACAGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCTCCAGGCGCTGCAGTTc                          >  2:3428265/1‑151 (MQ=255)
                                                                                                                           gtGCACAGGGGAGGCTGCGCC‑A‑‑GGGGGGGGTACAGAACGAGCATTTGGGGTGTGACGCACCAGGTGCAGGTCATCGATGGCCGGTGCTGGGCCAGAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCTCCAGGCGCTGCAGTTcc                         <  2:4292342/150‑1 (MQ=255)
                                                                                                                            gggacaGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCTCCAGGCGCTGCAGTTCCa                        <  2:6418058/148‑1 (MQ=255)
                                                                                                                              aacaGGGCAGGCTGCGCCGG‑‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCTCCAGGCGCTGCAGTTCCAgg                      <  1:979248/150‑1 (MQ=255)
                                                                                                                               acaGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCTCCAGGCGCTGCAGTTCCAggg                     >  2:4683033/1‑151 (MQ=255)
                                                                                                                                caGGGCAGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCTCCCGGCGCTGCAGTTCCAGCGt                    >  2:6206704/1‑151 (MQ=255)
                                                                                                                                      aGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCTCCAGGCGCTGCAGTTCCAGGGtcggttc              <  1:1389778/151‑1 (MQ=255)
                                                                                                                                      aGGCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGATGATCAGCTCGGCCAGCTCCAGGCGCTGCAGGTCCAGGGtcggttc              >  1:4858953/1‑151 (MQ=255)
                                                                                                                                        gCTGCGCC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCTCCAGGCGCTGCAGTTCCAGGGtcggttcgg            >  1:3020981/1‑151 (MQ=255)
                                                                                                                                           gcgcC‑AG‑GGGG‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCTCCAGGCGCTGCAGGTCCAGGGTCGGTTCGGTgg         >  2:252225/1‑151 (MQ=255)
                                                                                                                                            cgcC‑A‑‑GGGGGGGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCTCCAGGCGCTGCAGTTCCAGGGTCGGTTCGGTGGa        >  1:1942411/1‑151 (MQ=255)
                                                                                                                                            cgcC‑A‑‑GGGGGGGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCTCCAGGCGCTGCAGTTCCAGGGTCGGTTCGGTGGa        <  1:5129016/151‑1 (MQ=255)
                                                                                                                                                  gggggg‑gggtACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCTCCAGGCGCTGCAGTTCCAGGGTCGGTTCGGTGGACGTg    <  2:3791659/149‑1 (MQ=255)
                                                                                                                                                    gggg‑gggtACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCGCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCTCCAGGCGCTGCAGGTCCAGGGGCGGTTCGGGGGACGTGGc  >  2:2793460/1‑151 (MQ=255)
                                                                                                                                                       |                                                                                                                                                    
TCGGGGCTGGCATCCAGCCTCACGGCACTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCTTGGGCAAGGATGCACAGGGCAGGCTGCGCC‑A‑‑GGGT‑GGGTACAGAACGCACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGGCCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCTCCAGGCGCTGCAGTTCCAGGGTCGGTTCGGTGGACGTGGC  >  CP009974/3297044‑3297339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑