Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 3,721,374 A→G intergenic (+316/+51) RPPX_16570 → / ← RPPX_16575 glutathione S‑transferase/glutaredoxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099743,721,3740AG83.3% 297.3 / 48.7 144intergenic (+316/+51)RPPX_16570/RPPX_16575glutathione S‑transferase/glutaredoxin
Reads supporting (aligned to +/- strand):  ref base A (15/9);  new base G (65/55);  total (80/64)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

TTAGGGCCTGGCTTGAGCGGGTCAGCAGCCACCCGCGGCATGTGCCGATGGTGGGCTGAAAACCGACCTGTTTTCCAGGCGCTTGCGCAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCAGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGCCGGCTTCTTCAATGCGCAGGCATGGC  >  CP009974/3721228‑3721521
                                                                                                                                                  |                                                                                                                                                   
ttAGGGCCTGGCTTGAGCGGGTCAGCAGCCACCCGCGGCATGTGCCGATGGTGGGCTGAAAACCGACCTGTTTTCCAGGCGCTTGCGCAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGc                                                                                                                                                 <  1:4844703/151‑1 (MQ=18)
   gggCCTGGCTTGAGCGGGTCAGCAGCCACCCGCGGCATGTGCCGATGGTGGGCTGAAAACCGACCTGTTTTCCAGGCGCTTGCGCAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCtn                                                                                                                                              <  1:4340921/151‑2 (MQ=14)
     gCCTGGCTTGAGCGGGTCAGCAGCCACCCGCGGCATGTGCCGATGGTGGGCTGAAAACCGACCTGTTTTCCAGGCGCTTGCGCAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCt                                                                                                                                            >  2:3995339/1‑151 (MQ=17)
      ccTGGCTTGAGCGGGTCAGCAGCCACCCGCGGCATGTGCCGATGGTGGGCTGAAAACCGACCTGTTTTCCAGGCGCTTGCGCAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCtt                                                                                                                                           >  2:3157395/1‑151 (MQ=17)
        tGGCTTGAGCGGGTCAGCAGCCACCCGCGGCATGTGCCGATGGTGGGCTGAAAACCGACCTGTTTTCCAGGCGCTTGCGCAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTcg                                                                                                                                         >  2:2182071/1‑151 (MQ=17)
           cTTGAGCGGGTCAGCAGCCACCCGCGGCATGTGCCGATGGTGGGCTGAAAACCGACCTGTTTTCCAGGCGCTTGCGCAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCgg                                                                                                                                      >  2:1504684/1‑151 (MQ=14)
            ttGAGCGGGTCAGCAGCCACCCGCGGCATGTGCCGATGGTGGGCTGAAAACCGACCTGTTCTCCAGGCGCTTGCGCAATCCTTGTGGGAGCGGGCGTGCCCGCCAACACGGGCGCCGCCCGTGCCAACCACCACGGCCCCCGCTTCCCggg                                                                                                                                     >  2:408219/1‑151 (MQ=11)
             tGAGCGGGTCAGCAGCCACCCGCGGCATGTGCCGATGGTGGGCTGAAAACCGACCTGTTTTCCAGGCGCTTGCGCAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGc                                                                                                                                    >  1:5786081/1‑151 (MQ=11)
             tGAGCGGGTCAGCAGCCACCCGCGGCATGTGCCGATGGTGGGCTGAAAACCGACCTGTTTTCCAGGCGCTTGCGCAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGc                                                                                                                                    >  1:5786615/1‑151 (MQ=11)
                                                                   cTGTTTTCCAGGCGCTTGCGCAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTc                                                                              <  2:4138613/151‑1 (MQ=11)
                                                                    tGTTTTCCAGGCGCTTGCGCAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCa                                                                             <  2:1572962/151‑1 (MQ=14)
                                                                    tGTTTTCCAGGCGCTTGCGCAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCa                                                                             >  2:5706880/1‑151 (MQ=14)
                                                                     gTTTTCCAGGCGCTTGCGCAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCAGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACCAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCAt                                                                            >  2:1297352/1‑151 (MQ=14)
                                                                       tttCCAGGCGCTTGCGCAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATcc                                                                          <  2:4809598/151‑1 (MQ=18)
                                                                          ccAGGCGCTTGCGCAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAgg                                                                       >  2:1403373/1‑151 (MQ=25)
                                                                          ccAGGCGCTTGCGCAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAgg                                                                       <  1:6457920/151‑1 (MQ=25)
                                                                           cAGGCGCTTGCGCAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGTGCGCAGCCCGTGCCAACCACCGCGGTTCCTGCTTCGCGGGCTGGCCCGCTCCCACAAGGGATCTGCCGCGTCAGTCAGCTGGCGGCAAACCGCTCATGCAGGt                                                                      >  1:931617/1‑151 (MQ=11)
                                                                            aGGCGCTTGCGCAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTa                                                                     <  2:5831701/151‑1 (MQ=37)
                                                                              gcgcTTGCGCAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAg                                                                   >  1:3070462/1‑151 (MQ=255)
                                                                               cgcTTGCGCAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGc                                                                  <  1:1510091/151‑1 (MQ=255)
                                                                               cgcTTGCGCAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCGCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGc                                                                  >  2:3784715/1‑151 (MQ=255)
                                                                                gcTTGCGCAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCg                                                                 >  2:5979594/1‑151 (MQ=255)
                                                                                 cTTGCGCAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCga                                                                <  2:4861253/151‑1 (MQ=255)
                                                                                  ttGCGCAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCgat                                                               >  1:741444/1‑151 (MQ=255)
                                                                                   tGCGCAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCgatg                                                              <  2:582371/151‑1 (MQ=255)
                                                                                    gcgcAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCAGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCgatga                                                             <  2:5605989/151‑1 (MQ=255)
                                                                                     cgcAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCAGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCgatgat                                                            >  1:1120352/1‑151 (MQ=255)
                                                                                      gcAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATc                                                           >  1:1761616/1‑151 (MQ=255)
                                                                                      gcAATCCTTGTGGGAGCGGGCGTGCCCGCGAACAAGGGCGCCGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATc                                                           <  1:282074/151‑1 (MQ=255)
                                                                                        aaTCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAgg                                                                       <  1:4742139/137‑1 (MQ=37)
                                                                                        aaTCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCAGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAgg                                                                       >  2:4742139/1‑137 (MQ=37)
                                                                                           ccTTGTGGGAGCGGCCGTGCCCGCGAACACCGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCtt                                                      <  1:502068/151‑1 (MQ=255)
                                                                                               gtgGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGAc                                                  <  1:181776/151‑1 (MQ=255)
                                                                                                  ggAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCa                                               <  1:859170/151‑1 (MQ=255)
                                                                                                   gAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCAt                                              >  1:6158550/1‑151 (MQ=255)
                                                                                                    aGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATa                                             >  2:2121393/1‑151 (MQ=255)
                                                                                                     gCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATAc                                            >  2:2027084/1‑151 (MQ=255)
                                                                                                     gCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATAc                                            <  2:380542/151‑1 (MQ=255)
                                                                                                      cgcgCGAGCCCGCAAACACGGGCGCAGCCCGTGCCAACCACCCCGACGCGTGCTCCGCGGGCCCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACa                                           <  1:5632276/148‑1 (MQ=18)
                                                                                                      cGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGGTCATCCAGGTACGCGATGGTCGCCGTGGACTCCTACa                                           >  2:416848/1‑151 (MQ=14)
                                                                                                      cGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCAGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACa                                           <  1:4058136/151‑1 (MQ=255)
                                                                                                       gggCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACAt                                          <  2:5555523/151‑1 (MQ=255)
                                                                                                       gggCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCAGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTGAGCCGTGACCGCCTTGGCCTCATACac                                          >  2:4208386/1‑150 (MQ=14)
                                                                                                       gggCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCAGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACAt                                          <  1:1676763/151‑1 (MQ=255)
                                                                                                        ggCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATc                                         <  2:2973211/151‑1 (MQ=255)
                                                                                                          cGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGCTCGCCTTGGACTCATACATCCa                                       <  1:3388177/151‑1 (MQ=255)
                                                                                                           gTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAg                                      <  1:1935888/151‑1 (MQ=255)
                                                                                                           gTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAg                                      <  1:3564803/151‑1 (MQ=255)
                                                                                                           gTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAg                                      <  2:5268679/151‑1 (MQ=255)
                                                                                                           gTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAg                                      >  1:4173701/1‑151 (MQ=255)
                                                                                                            tGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAgg                                     >  2:364976/1‑151 (MQ=255)
                                                                                                             gCCAGCGAACACGGACGGCGCCCGCGCCGACCTCCGCGGTGCCCCCTCCGCGGGCTCGTCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGt                                    <  2:2876844/151‑1 (MQ=18)
                                                                                                              cccGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCTCTCATCCAGGTAAGCGATGATCGCCTTTGACTCATACATCCAGGTc                                   >  2:354653/1‑151 (MQ=255)
                                                                                                              cccGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATcc                                        <  1:5373466/146‑1 (MQ=255)
                                                                                                              cccGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATcc                                        >  2:5373466/1‑146 (MQ=255)
                                                                                                              cccGCGAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTc                                   >  1:2743723/1‑151 (MQ=255)
                                                                                                              cccGCGAACACGGGCGCAGCCCGTGCCAACCACCGCAGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCg                                                          <  1:6340327/128‑1 (MQ=255)
                                                                                                              cccGCGAACACGGGCGCAGCCCGTGCCAACCACCGCAGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCg                                                          >  2:6340327/1‑128 (MQ=255)
                                                                                                                cgcgAACACGGGCGCAGCCCGTGCCAACCACCGCAGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATAc                                            <  1:2079878/140‑1 (MQ=255)
                                                                                                                 gcgAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCAcc                                >  1:5726249/1‑151 (MQ=255)
                                                                                                                   gAACACGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCtt                              >  1:1744571/1‑151 (MQ=255)
                                                                                                                      cacGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGCTCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAACCGATGATCGCCTTGGACTCATCCCTCCACGTCACCTTccc                           >  2:4354419/1‑151 (MQ=18)
                                                                                                                      cacGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGcc                           >  1:245451/1‑151 (MQ=255)
                                                                                                                      cacGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGcc                           <  1:2527226/151‑1 (MQ=255)
                                                                                                                      cacGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGcc                           >  2:5699963/1‑151 (MQ=255)
                                                                                                                        cGGGCGCAGCGCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGCCgg                         <  2:3031916/151‑1 (MQ=255)
                                                                                                                        cGGGCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGAc                                                  <  2:2840513/126‑1 (MQ=255)
                                                                                                                         gggCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGCCGGc                        >  1:3109733/1‑151 (MQ=255)
                                                                                                                          ggCGCAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGCCGGct                       <  2:766308/151‑1 (MQ=255)
                                                                                                                            cgcAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGCCGGcttc                     >  1:1429950/1‑151 (MQ=255)
                                                                                                                             gcAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGCCGGcttct                    <  1:1268082/151‑1 (MQ=255)
                                                                                                                              cAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATTGCGGCAAACCGCTCATCCAGGTAAGCGATGATTGCCTTGGACTCATACATCCAGGTCACCTTGCCGGcttctt                   >  1:2834522/1‑151 (MQ=255)
                                                                                                                              cAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGCCGGcttctt                   <  2:4345793/151‑1 (MQ=255)
                                                                                                                              cAGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGCCGGcttctt                   >  1:4433099/1‑151 (MQ=255)
                                                                                                                               aGCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTTCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGCCGGCttcttc                  >  1:2846579/1‑151 (MQ=255)
                                                                                                                                gCCCGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGCCGGCTTCTTCa                 <  1:1125419/151‑1 (MQ=255)
                                                                                                                                 cccGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGCCGGCTTCTTCaa                >  2:1821622/1‑151 (MQ=255)
                                                                                                                                  ccGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGCCGGCTTCTTCAAt               >  2:5560949/1‑151 (MQ=255)
                                                                                                                                   cGTGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGCCGGCTTCTTCAATg              <  2:1429950/151‑1 (MQ=255)
                                                                                                                                   cGTGCCAACCACCGCAGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGCCGGCTTCTTCAATg              <  2:1120352/151‑1 (MQ=255)
                                                                                                                                    gTGCCAACCACCGCAGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACGGATACACCCCGGCCCCCTTGCCGGCTTCTTCAATgc             >  2:4579302/1‑151 (MQ=21)
                                                                                                                                     tGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGCCGGCTTCTTCAATgcg            <  1:3624714/151‑1 (MQ=255)
                                                                                                                                     tGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGCCGGCTTCTTCAATgcg            >  1:446400/1‑151 (MQ=255)
                                                                                                                                     tGCCAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGCCGGCTTCTTCAATgcg            >  1:2357357/1‑151 (MQ=255)
                                                                                                                                     tGCCAACCACCGCAGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCCGGTAGGCGAGGATCGCCTTGGGCGCATACACACAGGGCCGCGTGCTGGCTGCCTGAATgcc            >  2:3985983/1‑150 (MQ=14)
                                                                                                                                       ccAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGCCGGCTTCCTCACTgcccc          >  2:4601039/1‑148 (MQ=255)
                                                                                                                                        cAACCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGCCTCATACATCCAGGCCACCTTGCCGGCTTCTTCACTgcgccg         >  2:3486398/1‑149 (MQ=255)
                                                                                                                                         aaCCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGCCGGCTTCTTCAATGCGCAgg        >  1:1093454/1‑151 (MQ=255)
                                                                                                                                         aaCCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGCCGGCTTCTTCAATGCGCAgg        >  1:4580223/1‑151 (MQ=255)
                                                                                                                                         aaCCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGCCGGCTTCTTCAATGCGCAgg        >  1:5413481/1‑151 (MQ=255)
                                                                                                                                         aaCCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGCCGGCTTCTTCAATGCGCAgg        <  2:1190518/151‑1 (MQ=255)
                                                                                                                                         aaCCACCGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCCTGGACTCATACATCCAGGGCACCCTGCCGGCTACTTCAATGAGCAgg        >  2:5259405/1‑151 (MQ=21)
                                                                                                                                         aaCCACCGCAGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCt                               <  1:716583/128‑1 (MQ=255)
                                                                                                                                         aaCCACCGCAGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCt                               >  2:716583/1‑128 (MQ=255)
                                                                                                                                          accaccGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCACACCGCTCATCCAGGTAAGCGATGCTCGCCTTGGACTCATACATCCAGGTCACCTTGCCGCCCTCTTCAATGCGCAGGc       >  2:3288560/1‑151 (MQ=255)
                                                                                                                                           ccaccGCAGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCtt                                                      <  1:3389789/103‑1 (MQ=255)
                                                                                                                                           ccaccGCAGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCtt                                                      >  2:3389789/1‑103 (MQ=255)
                                                                                                                                             accGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGCCGGCTTCTTCAATGCGCAGGCATg    >  2:5460170/1‑151 (MQ=255)
                                                                                                                                              ccGCGGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGCCGGCTTCTTCAATGCGCAGGCATgg   <  1:3896991/151‑1 (MQ=255)
                                                                                                                                               cgcgGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGCCGGCTTCTTCAATGCGCAGGCATGGc  >  1:3669943/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TTAGGGCCTGGCTTGAGCGGGTCAGCAGCCACCCGCGGCATGTGCCGATGGTGGGCTGAAAACCGACCTGTTTTCCAGGCGCTTGCGCAATCCTTGTGGGAGCGGGCGTGCCCGCGAACACGGGCGCAGCCCGTGCCAACCACCGCAGTGCCTGCTTCGCGGGCTCGCCCGCTCCCACAAGGGATCTGCAGCGTCAGTCAGATGGCGGCAAACCGCTCATCCAGGTAAGCGATGATCGCCTTGGACTCATACATCCAGGTCACCTTGCCGGCTTCTTCAATGCGCAGGCATGGC  >  CP009974/3721228‑3721521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑