Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 3,971,951 G→T S9S (TCC→TCA)  RPPX_17675 ← hemolysin D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099743,971,9510GT98.5% 1165.3 / NA 341S9S (TCC→TCA) RPPX_17675hemolysin D
Reads supporting (aligned to +/- strand):  ref base G (4/1);  new base T (174/160);  total (180/161)
Fisher's exact test for biased strand distribution p-value = 3.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 347 total aligned reads displayed.

GTAGGCATCGTCGGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGGGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCCGCGAACACGGGCGA  >  CP009974/3971801‑3972098
                                                                                                                                                      |                                                                                                                                                   
   ggCATCGTCGGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAg                                                                                                                                                  <  2:2303361/151‑1 (MQ=255)
      aTCGTCGGTGGTTTCGAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGt                                                                                                                                               <  2:1518871/151‑1 (MQ=255)
      aTCGTCGGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGt                                                                                                                                               >  1:2128864/1‑151 (MQ=255)
        cgtcgGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGttt                                                                                                                                             >  1:5522779/1‑151 (MQ=255)
              tggtTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTAc                                                                                                                                       <  1:3538483/151‑1 (MQ=255)
               ggtTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACg                                                                                                                                      <  1:1203304/151‑1 (MQ=255)
               ggtTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACg                                                                                                                                      <  1:565946/151‑1 (MQ=255)
               ggtTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACg                                                                                                                                      >  2:313054/1‑151 (MQ=255)
                 tttCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGc                                                                                                                                    <  1:3806547/151‑1 (MQ=255)
                  ttCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCa                                                                                                                                   >  2:2625968/1‑151 (MQ=255)
                  ttCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCa                                                                                                                                   >  2:2134057/1‑151 (MQ=255)
                  ttCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGGGAGGGTTTGTCTACGGCa                                                                                                                                   >  2:602695/1‑151 (MQ=255)
                   tCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCaa                                                                                                                                  >  2:5076915/1‑151 (MQ=255)
                       gATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACAtt                                                                                                                              <  1:2234605/151‑1 (MQ=255)
                        aTAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTc                                                                                                                             >  1:2564175/1‑151 (MQ=255)
                          aGCGGCCGGTGCTGAACCGGAAGCTGGTGACGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCGGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCAt                                                                                                                           <  2:2882086/151‑1 (MQ=255)
                           gCGGCCGGTGCTGAACCGGTAGGTGGTGAGGGCCGGCAGGGTGGTTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCAtg                                                                                                                          <  2:5306593/151‑1 (MQ=255)
                             ggCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCAtggt                                                                                                                        >  1:1597056/1‑151 (MQ=255)
                              gCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCAtggtt                                                                                                                       >  1:4189133/1‑151 (MQ=255)
                                     cTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTcc                                                                                                                <  1:1734136/151‑1 (MQ=255)
                                      tGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCa                                                                                                               >  1:3415093/1‑151 (MQ=255)
                                        aaCCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCATGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCAtt                                                                                                             <  2:4994241/151‑1 (MQ=255)
                                        aaCCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCAtt                                                                                                             >  1:5260771/1‑151 (MQ=255)
                                         aCCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATtc                                                                                                            <  2:822143/151‑1 (MQ=255)
                                          ccAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCCACCTTCATGGTTGGTTTCCATtct                                                                                                           >  2:5744159/1‑151 (MQ=255)
                                           cAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCTAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGGTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCACCATGCATGGTTGGATTGGATtctt                                                                                                          >  1:1876685/1‑150 (MQ=255)
                                           cAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATtctc                                                                                                          <  1:698098/151‑1 (MQ=255)
                                            aGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCg                                                                                                         <  2:1888189/151‑1 (MQ=255)
                                             gTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGa                                                                                                        >  1:6213885/1‑151 (MQ=255)
                                              tAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTCGGTTTCCATTCTCGaa                                                                                                       >  2:5185318/1‑151 (MQ=255)
                                               aGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAGCATTCATGGTTGGTTTCCATTCTCGAAt                                                                                                      <  2:2755722/151‑1 (MQ=255)
                                                   ggTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTggg                                                                                                  <  1:4705133/151‑1 (MQ=255)
                                                   ggTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTggg                                                                                                  >  1:2699503/1‑151 (MQ=255)
                                                    gTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGc                                                                                                 >  2:4065833/1‑151 (MQ=255)
                                                     tGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGcc                                                                                                <  2:102832/151‑1 (MQ=255)
                                                        aGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCctgc                                                                                             <  1:4418761/151‑1 (MQ=255)
                                                           ccAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATGCATGGTTGGTTTCCCTTCTCGAATTGGGCCGgctgg                                                                                          >  2:4625520/1‑151 (MQ=255)
                                                            cagcagGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTgctggc                                                                                         >  2:5663793/1‑151 (MQ=255)
                                                            cagcagGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTgctggc                                                                                         >  1:639600/1‑151 (MQ=255)
                                                             agcagGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTgctggct                                                                                        <  1:533877/151‑1 (MQ=255)
                                                              gcagGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTgctggctg                                                                                       <  1:5724739/151‑1 (MQ=255)
                                                              gcagGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTgctggctg                                                                                       <  2:3514676/151‑1 (MQ=255)
                                                              gcagGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTgctggctg                                                                                       <  2:5542431/151‑1 (MQ=255)
                                                                 gggTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAGCATTCATGGTTGGTTTCCCTTCTCGAAATGGGCCTGCTGGCTGTCg                                                                                    >  2:6321648/1‑151 (MQ=255)
                                                                 gggTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTTGCTGTCg                                                                                    <  2:1609987/151‑1 (MQ=255)
                                                                 gggTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCg                                                                                    >  1:5803634/1‑151 (MQ=255)
                                                                 gggTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCg                                                                                    <  2:4846460/151‑1 (MQ=255)
                                                                     gATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTtg                                                                                <  2:5838500/151‑1 (MQ=255)
                                                                      aTTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTtgt                                                                               <  1:920529/151‑1 (MQ=255)
                                                                      aTTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTtgt                                                                               <  2:1170998/151‑1 (MQ=255)
                                                                       ttGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTTTCGGTtgtg                                                                              >  1:2044302/1‑151 (MQ=255)
                                                                          cGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGa                                                                           >  1:2901665/1‑151 (MQ=255)
                                                                             ccAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGcc                                                                        >  2:6492826/1‑151 (MQ=255)
                                                                               aGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCAc                                                                      <  1:312489/151‑1 (MQ=255)
                                                                               aGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCAc                                                                      >  2:670377/1‑151 (MQ=255)
                                                                                 gCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTg                                                                    <  2:1967340/151‑1 (MQ=255)
                                                                                 gCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTg                                                                    >  1:1069190/1‑151 (MQ=255)
                                                                                     ccGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCtt                                                                <  2:2487423/151‑1 (MQ=255)
                                                                                      cGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTg                                                               >  2:5261615/1‑151 (MQ=255)
                                                                                      cGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTg                                                               <  1:6053616/151‑1 (MQ=255)
                                                                                        cTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCt                                                             <  1:1617600/151‑1 (MQ=255)
                                                                                        cTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCt                                                             <  1:1412763/151‑1 (MQ=255)
                                                                                        cTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCt                                                             <  2:3376998/151‑1 (MQ=255)
                                                                                          ggCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCg                                                           <  1:4994155/151‑1 (MQ=255)
                                                                                           gCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGa                                                          <  1:4527758/151‑1 (MQ=255)
                                                                                                 gttGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTGTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTa                                                    <  2:383311/150‑1 (MQ=255)
                                                                                                   tGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTaaa                                                  <  2:132420/151‑1 (MQ=255)
                                                                                                     ccAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAg                                                >  2:3853386/1‑151 (MQ=255)
                                                                                                      cAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGc                                               >  2:1446557/1‑151 (MQ=255)
                                                                                                       aGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCt                                              <  2:2976794/151‑1 (MQ=255)
                                                                                                        ggcggcGACGAGTTTTAGCGCTAGGTCGGTGGTCAGTTGTGGCGGGTGAGGGTTTGTCTCTGGTATCATTCTTGGTTGGTTTCCATTCTGGGATTGGGGCTGCTGGCGGTCGGTTGTGGGAGCCACTGTCTTGCTGGCTTTCTAGGAGCTg                                             <  2:1711297/151‑1 (MQ=255)
                                                                                                          cggcgACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGgc                                           <  2:590160/151‑1 (MQ=255)
                                                                                                           ggcgACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAACAGCTGgcg                                          >  1:5899612/1‑151 (MQ=255)
                                                                                                              gACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGa                                       >  1:2090732/1‑151 (MQ=255)
                                                                                                                cGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATc                                     >  1:4056270/1‑151 (MQ=255)
                                                                                                                cGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATc                                     <  1:3853386/151‑1 (MQ=255)
                                                                                                                cGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATc                                     >  1:2461309/1‑151 (MQ=255)
                                                                                                                 gggTTTTAGCGCGCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGCATTGGGCCTGCTGGCTGTCGGTTCTGGGAGCCACTGTCTTGTCCTATTTCTACAAGCTGGCGCGATcc                                    >  2:4488013/1‑151 (MQ=255)
                                                                                                                       tAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTgg                              >  2:3935754/1‑151 (MQ=255)
                                                                                                                        ggcgcTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTggg                             <  2:4949226/150‑1 (MQ=255)
                                                                                                                        aGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTggg                             >  1:3365916/1‑151 (MQ=255)
                                                                                                                         gcgcTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGa                            <  1:3251366/151‑1 (MQ=255)
                                                                                                                          cgcTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGgcg                                          <  2:2546228/136‑1 (MQ=255)
                                                                                                                          cgcTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAg                           >  1:2971165/1‑151 (MQ=255)
                                                                                                                          cgcTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAg                           >  1:2345745/1‑151 (MQ=255)
                                                                                                                            cTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCg                         <  1:3489962/151‑1 (MQ=255)
                                                                                                                             tCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCgg                        >  2:4173363/1‑151 (MQ=255)
                                                                                                                              cGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCggg                       >  1:4843939/1‑151 (MQ=255)
                                                                                                                                 ccGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGTGAGTCACTGTCTTGCCTGATTTCTAAAAGCTGGCGCGCTCCGTGTGGGAGCGCGCtt                    >  2:6157695/1‑151 (MQ=255)
                                                                                                                                 ccGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCtt                    >  1:5432890/1‑151 (MQ=255)
                                                                                                                                     tGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGccc                <  2:4381607/151‑1 (MQ=255)
                                                                                                                                     tGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTAGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGccc                >  1:3695080/1‑151 (MQ=255)
                                                                                                                                      gTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTCAAAGCTGGCGCGCTCCGTGTGGGAGCGGCCTGGCCcg               >  2:4333651/1‑151 (MQ=255)
                                                                                                                                      gTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCcg               <  2:5449279/151‑1 (MQ=255)
                                                                                                                                      gTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCcg               <  1:1795793/151‑1 (MQ=255)
                                                                                                                                          aGTTTCGGGGGGTGACGGTCTGTCGACGGCAGGATTCATGGTTGGTTTCCATTCTCGACTTGGGCCTGCTGGCTGTCGGTTGCGGGAGGCCCTCCCTTGCTCGATTTCTCAAAGCTGGCGCGATCCGTGTGGAATCGTGCTTGCCCGCGaa           >  2:3975411/1‑151 (MQ=255)
                                                                                                                                            tttCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCCGCGAaca         >  1:88585/1‑151 (MQ=255)
                                                                                                                                             ttCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCCGCGAacac        <  2:2358574/151‑1 (MQ=255)
                                                                                                                                               cggcggGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCCGCGAACACgg      <  1:4657938/151‑1 (MQ=255)
                                                                                                                                                   gggtgAGGGTTTGTCTACGGCAACACTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCCGCGAACACGGgaga  >  1:4331157/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
GTAGGCATCGTCGGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGGGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCCGCGAACACGGGCGA  >  CP009974/3971801‑3972098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑