Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 542,098 A→T intergenic (‑359/+170) argD ← / ← RPPX_02300 acetylornithine aminotransferase/AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009974542,0980AT88.2% 81.2 / 8.7 51intergenic (‑359/+170)argD/RPPX_02300acetylornithine aminotransferase/AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/6);  new base T (3/42);  total (3/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTCCCTCCTGTGCCTGGCGCGCGCGACCGCCTTGTTTGTACGACGCGATATCGAGGCATGCGCCAAGGCGTTCGCGCGCAAATGCCACAGGCATAACTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAA  >  CP009974/541950‑542193
                                                                                                                                                    |                                                                                               
gttGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTtcg                                                                                               <  2:3180494/150‑1 (MQ=18)
 ttGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTtcga                                                                                              <  2:5540772/151‑1 (MQ=255)
 ttGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTtcga                                                                                              <  1:6409941/151‑1 (MQ=255)
  tgtgAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTtcgac                                                                                             >  1:3553026/1‑151 (MQ=18)
  ggtgAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTtcgac                                                                                             <  2:2128398/150‑1 (MQ=18)
   gtgAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGCCCGCCTTGTTTGTtcgacg                                                                                            >  2:3828930/1‑151 (MQ=18)
   gtgAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTtcgacg                                                                                            <  2:2049086/151‑1 (MQ=18)
   gtgAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTtcgacg                                                                                            <  1:968381/151‑1 (MQ=18)
    tgAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTtcgacgg                                                                                           <  1:2488163/151‑2 (MQ=18)
      aGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGa                                                                                         <  1:262799/151‑1 (MQ=18)
       ggggACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGat                                                                                        <  2:2749944/151‑1 (MQ=18)
         ggACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGatat                                                                                      <  2:3083254/151‑1 (MQ=18)
         ggACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGatat                                                                                      <  1:5345295/151‑1 (MQ=18)
         ggACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGatat                                                                                      <  1:5427732/151‑1 (MQ=18)
           aCAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCg                                                                                    >  2:2902108/1‑151 (MQ=17)
             aGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAg                                                                                  <  1:5259976/151‑1 (MQ=17)
              ggATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAgg                                                                                 <  1:1809061/151‑1 (MQ=17)
                 tGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggtgt                                                                              <  2:3280355/151‑4 (MQ=17)
                 tGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggtgt                                                                              <  2:3281194/151‑4 (MQ=17)
                 tGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggtgt                                                                              <  1:5240683/151‑4 (MQ=17)
                 tGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggtgt                                                                              <  1:4394828/151‑4 (MQ=17)
                 ggTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggtgt                                                                              <  2:3346110/150‑4 (MQ=17)
                  gTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggtgtg                                                                             <  1:3777902/151‑5 (MQ=17)
                  gTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggtgtg                                                                             <  1:1220467/151‑5 (MQ=17)
                    aCGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggtgtgcg                                                                           <  1:2686686/151‑7 (MQ=17)
                    aCGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggtgtgcg                                                                           <  1:3536528/151‑7 (MQ=17)
                       gCGATGTTGATGTGTTGGCGGGGAGATCGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCa                                                                        <  1:2712364/151‑1 (MQ=14)
                         gATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAg                                                                      <  1:536255/151‑1 (MQ=14)
                         gATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAg                                                                      <  1:5617892/151‑1 (MQ=14)
                         gATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAg                                                                      <  1:6289258/151‑1 (MQ=14)
                         gATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAg                                                                      <  1:2833822/151‑1 (MQ=14)
                         gATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAg                                                                      <  1:688319/151‑1 (MQ=14)
                          gtGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAgg                                                                     <  2:464662/150‑1 (MQ=14)
                          aTGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAgg                                                                     <  1:5530348/151‑1 (MQ=14)
                           ggTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGc                                                                    <  2:808804/150‑1 (MQ=14)
                             ttGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGt                                                                  <  1:223373/151‑1 (MQ=14)
                             ttGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGt                                                                  <  1:4516514/151‑1 (MQ=14)
                              tGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGtt                                                                 <  2:4202900/151‑1 (MQ=14)
                              ggATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGtt                                                                 <  2:4215284/150‑1 (MQ=14)
                               gATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTCCGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTc                                                                <  1:6774/151‑1 (MQ=14)
                               gATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTc                                                                <  2:4474404/151‑1 (MQ=14)
                               gATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTc                                                                <  1:6051069/151‑1 (MQ=14)
                                 tgtgTCGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTcgc                                                              <  1:3231737/151‑1 (MQ=11)
                                  gtgtTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTcgcg                                                             <  1:3654188/151‑1 (MQ=11)
                                   tgtTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTcgcgc                                                            <  1:2821053/151‑1 (MQ=11)
                                      tGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCa                                                         <  1:1943197/151‑1 (MQ=11)
                                        gCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCaaa                                                       <  1:3725168/151‑1 (MQ=11)
                                         cGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCAAAt                                                      >  2:5617581/1‑151 (MQ=11)
                                           tagtgaTCGAGCGCCGCCCGAGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTCCCTCCTGCACCAGTCGCGCGCGACCGCCTTGTTTGTACGACGCGATATCGAGACATGCGCCAAGGCGGTCGCGCGCAAAtcc                                                    <  2:2039311/147‑3 (MQ=1)
                                           gggAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCAAATGc                                                    <  1:3578539/151‑1 (MQ=11)
                                            ggAGCTCGAGGGCCGCCCGGGCGGCGCTTCGCGGGTGAGCCCGCTCCTACGCGTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCAAATGcc                                                   <  2:6244409/151‑1 (MQ=11)
                                                 gcgggcgcCGCCCGCGCGGCGCTTCGCGGGTAAGCCCGCTCCTACGTTTGTTTCGGGCCAGTCCATCCTGTGCCTGGCGCGCGCGACCGCCTTGTTTGTACGACGCGATATCGAGGCATGCACCAAGGCGTTCGCGCGCAAATCCCACAgg                                              <  2:5775045/147‑1 (MQ=1)
                                                                               gaagcccGCGCCGGCGCTTGGTTCGGGCGAGTCCCGCCTGCGGCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGGGTGCGCCAAGGCGTTCGCGCGCAAATGCCACAGGCATAACTGGCCCGAAACAAACGTAGGAGca                <  2:5083287/147‑2 (MQ=1)
                                                                                aaccccGCTCCTCCGTGTGTTTCGGGCCAGTCCCTCCTGTGCCTGGCGCGCGCGACCGCCTTGTTGGTACGACGCGATATCGAGACAGGCACCAAGGCGTTCGCGCGCGAATCCCACAGGCATAATTGGCCCGAAACAAACGTAGGAGCgg               <  2:3798679/148‑1 (MQ=1)
                                                                                aaccccGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCAAATGCCACAGGCATAACTGGCCCGAAACAAACGTAGGAGcag               <  1:4786093/148‑3 (MQ=1)
                                                                                 aatccGCTCCTACGTTTGTTTCGGGCCAGTCCATCCTGTGCCTGGCGCGCGCGACCGCCTTGTTTGTACGACGCGTTATCGAGGCATGCACCAAGGCGTTCGCGCGCAAATCCCACAGGCATAATTGGCCTGTTACAATCGTAGGTGCggg              <  2:5147399/148‑1 (MQ=1)
                                                                                      gccccgaCGTTTGTGCCGGGCCACCCCCCCCTGCGCCAGTCGCGCGCGACCGCCTTGTTTGTACGACGCCACATCGAGACATGCGCCAAGGCGGTCGCGCGCAAATCCCACAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTAc         <  1:1663145/145‑1 (MQ=1)
                                                                                         cccaCGTTTTTTCCGGGCCAGCCCCTCCTGTGCCTGGCGGGCGCGCCCGCCTTGTTGGTACGACGCGATATCGAGACAGGCACCAAGGCGTTCGCGCGCGAATCCCACAGGCATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCcg      <  1:5799229/148‑1 (MQ=1)
                                                                                             cgcttgtttCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCAAATGCCACAGGCATAACTGGCCCGAAACAAACGTAGGAGCAGGTTTACCCGCGaa  <  2:4757867/148‑1 (MQ=1)
                                                                                             cgcttgtttCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCAAATGCCACAGGCATAACTGGCCCGAAACAAACGTAGGAGCAGGTTTACCCGCGaa  >  1:2408678/4‑151 (MQ=1)
                                                                                                                                                    |                                                                                               
ATTGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTCCCTCCTGTGCCTGGCGCGCGCGACCGCCTTGTTTGTACGACGCGATATCGAGGCATGCGCCAAGGCGTTCGCGCGCAAATGCCACAGGCATAACTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAA  >  CP009974/541950‑542193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑