Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 4,147,416 C→G intergenic (+668/‑353) RPPX_18425 → / → RPPX_18430 enoyl‑CoA hydratase/conjugal transfer protein TraR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099744,147,4160CG77.0% 391.3 / 99.5 153intergenic (+668/‑353)RPPX_18425/RPPX_18430enoyl‑CoA hydratase/conjugal transfer protein TraR
Reads supporting (aligned to +/- strand):  ref base C (23/12);  new base G (81/36);  total (104/49)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 154 total aligned reads displayed.

AAAAAACGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTT  >  CP009974/4147277‑4147563
                                                                                                                                           |                                                                                                                                                   
                                      cgctCCGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatca                                                                                                   <  1:4247091/151‑1 (MQ=11)
                                      cgctCCGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatca                                                                                                   <  2:4107609/151‑1 (MQ=11)
                                       gctCCGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaa                                                                                                  >  1:4776184/1‑151 (MQ=11)
                                        ctCCGGGGGCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaag                                                                                                 <  1:2275792/151‑1 (MQ=11)
                                         tCCGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTCCGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaaga                                                                                                >  2:1731338/1‑151 (MQ=11)
                                         tCCGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaaga                                                                                                >  1:3105751/1‑151 (MQ=11)
                                         cccGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaaga                                                                                                <  1:1142955/150‑1 (MQ=11)
                                          ccGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagat                                                                                               <  1:1248904/151‑1 (MQ=11)
                                           cGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCCGAAGTCGCCaagatcaagatcaagatc                                                                                              >  2:5714002/1‑151 (MQ=11)
                                            ggggTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatca                                                                                             >  2:631190/1‑151 (MQ=11)
                                             gggTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaa                                                                                            >  2:1350073/1‑151 (MQ=11)
                                              ggTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaag                                                                                           <  2:4263890/151‑1 (MQ=11)
                                               gTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagaccaaga                                                                                          >  2:2352017/1‑151 (MQ=11)
                                                  cccTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatca                                                                                       >  1:5218294/1‑151 (MQ=11)
                                                     tctcTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaaga                                                                                    <  1:2952365/148‑1 (MQ=14)
                                                        cTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAagatcaagatcaagatcaagatcaagatca                                                                                 >  1:4971605/1‑151 (MQ=14)
                                                        cTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAagatcaagatcaagatcaagatcaagatca                                                                                 >  1:4476399/1‑151 (MQ=14)
                                                          ccGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaag                                                                               <  1:708779/151‑2 (MQ=11)
                                                          ccGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaag                                                                               >  1:3992670/1‑150 (MQ=11)
                                                           cGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaaga                                                                              >  2:310504/1‑149 (MQ=11)
                                                           cGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaaga                                                                              <  1:1512919/151‑3 (MQ=11)
                                                             gCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaagatc                                                                            >  2:2893333/1‑147 (MQ=11)
                                                              ccTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaagatca                                                                           >  2:6094661/1‑146 (MQ=11)
                                                               cTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggca                                                                           <  1:4484984/151‑1 (MQ=2)
                                                               cTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAACATCAAGATcaacggca                                                                           >  2:4062177/1‑151 (MQ=9)
                                                                  gCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacg                                                                        >  2:1715147/1‑151 (MQ=18)
                                                                    tCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggc                                                                      >  2:355622/1‑151 (MQ=21)
                                                                      ccGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaa                                                                    >  1:3591101/1‑151 (MQ=255)
                                                                        ggCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGCTCAAGATcaagggcaacggcaacg                                                                  >  1:6291127/1‑151 (MQ=255)
                                                                        ggCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaacg                                                                  >  1:4357953/1‑151 (MQ=255)
                                                                         gCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaacgg                                                                 >  2:1385835/1‑151 (MQ=255)
                                                                          cAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaacggc                                                                >  2:6226832/1‑151 (MQ=255)
                                                                              aCCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaacggcaacg                                                            >  2:5166213/1‑151 (MQ=255)
                                                                              aCCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaacggcaacg                                                            >  1:5366544/1‑151 (MQ=255)
                                                                                 gcgcCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAacggcaacggcaacggcaacggca                                                         >  2:1328644/1‑151 (MQ=255)
                                                                                 gcgcCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAacggcaacggcaacggcaacggca                                                         >  2:1334065/1‑151 (MQ=255)
                                                                                 gcgcCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCAGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGACCAAGATCAAGATCAAGATCAacggcaacggcaacggcaacggca                                                         >  1:4204095/1‑151 (MQ=255)
                                                                                  cgcCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAAcggcaacggcaacggcaacggcaa                                                        >  1:5741848/1‑151 (MQ=255)
                                                                                   gcCGAAGGCCCCAGCCGGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACggcaacggcaacggcaacggcaac                                                       <  2:4522831/151‑1 (MQ=255)
                                                                                    ccGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaaca                                                      >  1:3774431/1‑151 (MQ=255)
                                                                                    ccGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaaca                                                      >  2:1403822/1‑151 (MQ=255)
                                                                                     cGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacag                                                     >  1:2599255/1‑151 (MQ=255)
                                                                                     cGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacag                                                     <  1:4633095/151‑1 (MQ=255)
                                                                                       aaGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacagca                                                   >  1:1505544/1‑151 (MQ=255)
                                                                                        aGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacagcaa                                                  <  1:3436027/151‑1 (MQ=255)
                                                                                        aGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacagcaa                                                  >  2:1491585/1‑151 (MQ=255)
                                                                                         ggCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGACGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacagcacc                                                 >  2:3424982/1‑149 (MQ=255)
                                                                                          gCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacagcaaca                                                >  1:4903206/1‑151 (MQ=255)
                                                                                          gCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacagcaaca                                                >  2:4449059/1‑151 (MQ=255)
                                                                                              cATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCACcagcag                                            >  2:4898329/1‑151 (MQ=255)
                                                                                                tCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGc                                          >  1:2010986/1‑151 (MQ=255)
                                                                                                tCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGc                                          >  1:3431275/1‑151 (MQ=255)
                                                                                                 ccTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCg                                         >  2:4667705/1‑151 (MQ=255)
                                                                                                  cTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGa                                        >  1:6319752/1‑151 (MQ=255)
                                                                                                  cTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGa                                        <  2:5694074/151‑1 (MQ=255)
                                                                                                  cTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGa                                        <  2:4618435/151‑1 (MQ=255)
                                                                                                   tGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGaa                                       <  1:4010776/151‑1 (MQ=255)
                                                                                                   tGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGaa                                       >  1:967627/1‑151 (MQ=255)
                                                                                                   tGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGaa                                       >  1:967478/1‑151 (MQ=255)
                                                                                                   tGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGaa                                       <  1:5519052/151‑1 (MQ=255)
                                                                                                    ggggCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAAt                                      <  2:4924818/151‑1 (MQ=255)
                                                                                                    ggggCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAAt                                      >  1:6423674/1‑151 (MQ=255)
                                                                                                     gggCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATg                                     <  1:3192526/151‑1 (MQ=255)
                                                                                                     gggCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATg                                     <  2:296180/151‑1 (MQ=255)
                                                                                                     gggCCTCAGCGAGTGAGGGGCAACCATGCCCGTCACCTGCCTCCGCAAGGCCGGCGTTCGGCCTCATGAAGTCGCAAAGATCAAGATCAAGAACAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATg                                     <  2:3193494/151‑1 (MQ=14)
                                                                                                       gCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATggg                                   >  2:1323005/1‑151 (MQ=255)
                                                                                                        ccTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGc                                  >  1:4460648/1‑151 (MQ=255)
                                                                                                        ccTCAGCGCTTCACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCAGAAGTCGCGAAGATCAGGATCAAGATCAACATCAAGATCAACGGCAACGTCAACGACAACGGCAACAACAACAGCAGGCGAAGGGGc                                  >  2:6511050/1‑151 (MQ=14)
                                                                                                         cTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCa                                 >  2:2707576/1‑151 (MQ=255)
                                                                                                         cTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGCCGCGAAGCTCAAGATCAAGATCAAGATCCAGACCAACGGCAACGGCAACGGCAACGGCAACACCAACAGCAGGCGACTGGGcc                                 >  2:6492175/1‑150 (MQ=14)
                                                                                                           cAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACt                               >  2:1937085/1‑151 (MQ=255)
                                                                                                           cAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACt                               <  1:4803367/151‑1 (MQ=255)
                                                                                                           cAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCACGCGAATGGCCACt                               >  2:5239301/1‑151 (MQ=255)
                                                                                                              cgcTTGGCGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGcc                            <  1:5428957/151‑1 (MQ=255)
                                                                                                               gcTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCa                           <  1:4828053/151‑1 (MQ=255)
                                                                                                                cTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAg                          <  2:6317302/151‑1 (MQ=255)
                                                                                                                cTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAg                          >  2:3351071/1‑151 (MQ=255)
                                                                                                                 ttGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGa                         <  1:1385835/151‑1 (MQ=255)
                                                                                                                 ttGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGa                         >  2:2747283/1‑151 (MQ=255)
                                                                                                                   gACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAAt                       >  1:5883913/1‑151 (MQ=255)
                                                                                                                   gACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAAt                       <  1:1656249/151‑1 (MQ=255)
                                                                                                                   gACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAAt                       <  1:5294598/151‑1 (MQ=255)
                                                                                                                      gggCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATcac                    >  1:4710965/1‑151 (MQ=255)
                                                                                                                      gggCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATcac                    <  1:845282/151‑1 (MQ=255)
                                                                                                                       ggCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATcaca                   >  2:4763666/1‑151 (MQ=255)
                                                                                                                          aTCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGtt                >  1:2123893/1‑151 (MQ=255)
                                                                                                                          aTCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGtt                >  1:1353862/1‑151 (MQ=255)
                                                                                                                           tCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGttt               <  1:1906516/151‑1 (MQ=255)
                                                                                                                           tCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGttt               >  2:4521966/1‑151 (MQ=255)
                                                                                                                             cATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACAGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTtac             >  2:4813149/1‑151 (MQ=255)
                                                                                                                              aTGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTtact            >  1:6042146/1‑151 (MQ=255)
                                                                                                                               tGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTtacta           >  1:736871/1‑151 (MQ=255)
                                                                                                                                gCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTtactac          <  1:6226832/151‑1 (MQ=255)
                                                                                                                                 cccGCCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTactact         <  2:929698/151‑1 (MQ=255)
                                                                                                                                  ccGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAATATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTg        >  1:1582713/1‑151 (MQ=255)
                                                                                                                                  ccGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTg        >  1:2879370/1‑151 (MQ=255)
                                                                                                                                   cGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTgc       >  1:69863/1‑150 (MQ=255)
                                                                                                                                    gTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGc      >  1:3758972/1‑151 (MQ=255)
                                                                                                                                     tCACCTGCCACCGCAAGGCCTGCGTTCGGCCGCCTGATGTCGCGAAGATCAAGATCAAGATCAAGATCAAGACCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGcc     <  2:5115252/151‑1 (MQ=21)
                                                                                                                                      cACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGccc    >  1:3558280/1‑151 (MQ=255)
                                                                                                                                           |                                                                                                                                                   
AAAAAACGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTT  >  CP009974/4147277‑4147563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑