Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 4,147,431 A→G intergenic (+683/‑338) RPPX_18425 → / → RPPX_18430 enoyl‑CoA hydratase/conjugal transfer protein TraR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099744,147,4310AG81.8% 444.3 / 84.0 177intergenic (+683/‑338)RPPX_18425/RPPX_18430enoyl‑CoA hydratase/conjugal transfer protein TraR
Reads supporting (aligned to +/- strand):  ref base A (20/12);  new base G (95/49);  total (116/61)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 177 total aligned reads displayed.

GCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCAGCTGAGCTGGGGT  >  CP009974/4147311‑4147578
                                                                                                                        |                                                                                                                                                   
    cgctCCGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatca                                                                                                                  <  1:4247091/151‑1 (MQ=11)
    cgctCCGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatca                                                                                                                  <  2:4107609/151‑1 (MQ=11)
      ctCCGGGGGCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaag                                                                                                                <  1:2275792/151‑1 (MQ=11)
       tCCGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTCCGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaaga                                                                                                               >  2:1731338/1‑151 (MQ=11)
       tCCGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaaga                                                                                                               >  1:3105751/1‑151 (MQ=11)
       cccGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaaga                                                                                                               <  1:1142955/150‑1 (MQ=11)
        ccGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagat                                                                                                              <  1:1248904/151‑1 (MQ=11)
         cGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCCGAAGTCGCCaagatcaagatcaagatc                                                                                                             >  2:5714002/1‑151 (MQ=11)
          ggggTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatca                                                                                                            >  2:631190/1‑151 (MQ=11)
           gggTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaa                                                                                                           >  2:1350073/1‑151 (MQ=11)
            ggTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaag                                                                                                          >  1:565868/1‑151 (MQ=11)
             gTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagaccaaga                                                                                                         >  2:2352017/1‑151 (MQ=11)
               ccccTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTGGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatc                                                                                                       <  2:6250756/151‑1 (MQ=14)
                   tctcTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaaga                                                                                                   <  1:2952365/148‑1 (MQ=14)
                    ctcTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagagcaagatcaagat                                                                                                  >  1:4439/3‑151 (MQ=11)
                      cTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAagatcaagatcaagatcaagatcaagatca                                                                                                >  1:4971605/1‑151 (MQ=14)
                       tCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGTCCCCATCCT‑TGGTCTCAGCGCTTGACGGGCATCCAGGCCCGTCACCTCCCTCCGCAAGTCCTACGTTCGGCCTCCTTACGTCGCGAAaattaagatcaagatcacgatcaagatgaa                                                                                               >  1:1826265/1‑151 (MQ=11)
                       tCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaa                                                                                               >  2:1330586/1‑151 (MQ=11)
                         cGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaaga                                                                                             <  1:1512919/151‑3 (MQ=11)
                          ggCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaagat                                                                                            <  2:4878946/151‑4 (MQ=11)
                           gCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaagatc                                                                                           >  2:2893333/1‑147 (MQ=11)
                            ccTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaagatca                                                                                          >  2:6094661/1‑146 (MQ=11)
                             cTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggca                                                                                          <  1:4484753/151‑1 (MQ=2)
                               tGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGGCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaac                                                                                        >  2:6400260/1‑151 (MQ=9)
                                  tCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggc                                                                                     <  2:5490232/151‑1 (MQ=16)
                                     cGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaac                                                                                  <  1:3629621/151‑1 (MQ=255)
                                      ggCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGCTCAAGATcaagggcaacggcaacg                                                                                 >  1:6291127/1‑151 (MQ=255)
                                        cAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaacggc                                                                               >  1:4704107/1‑151 (MQ=255)
                                         aGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaacggca                                                                              >  2:3223495/1‑151 (MQ=255)
                                          ggACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaacggcaa                                                                             >  1:4792569/1‑151 (MQ=255)
                                            aCCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaacggcaacg                                                                           >  1:5366544/1‑151 (MQ=255)
                                               gcgcCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAacggcaacggcaacggcaacggca                                                                        >  2:1328644/1‑151 (MQ=255)
                                               gcgcCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCAGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGACCAAGATCAAGATCAAGATCAacggcaacggcaacggcaacggca                                                                        >  1:4204095/1‑151 (MQ=255)
                                               gcaccGAAGGCCCCATCCTGGAGCCTCATCGCTTGACGGACATCCATGCCCGTCACATACCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCAAAGATCAAGATCAAAATCAAGATCAAGATCAacggcaacggcaacggcaacggca                                                                        <  2:1465720/148‑1 (MQ=14)
                                                  ccGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaaca                                                                     >  2:1403822/1‑151 (MQ=255)
                                                  ccGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaaca                                                                     >  2:4166300/1‑151 (MQ=255)
                                                  ccGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaaca                                                                     >  1:3774431/1‑151 (MQ=255)
                                                   cGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacag                                                                    <  1:4633095/151‑1 (MQ=255)
                                                   cGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacag                                                                    >  1:5993350/1‑151 (MQ=255)
                                                   cGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacag                                                                    >  1:2599255/1‑151 (MQ=255)
                                                     aaGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacagca                                                                  >  1:1505544/1‑151 (MQ=255)
                                                      aGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCCACGGCAACGGCAACGGCAACGgcaacagcaa                                                                 >  2:5102256/1‑151 (MQ=255)
                                                      aGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacagcaa                                                                 <  1:3436027/151‑1 (MQ=255)
                                                        gCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacagcaaca                                                               >  2:4449059/1‑151 (MQ=255)
                                                            cATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCACcagcag                                                           >  2:4898329/1‑151 (MQ=255)
                                                              tCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGc                                                         >  1:3431275/1‑151 (MQ=255)
                                                                cTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGa                                                       <  2:4617081/151‑1 (MQ=255)
                                                                cTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGa                                                       <  2:4618435/151‑1 (MQ=255)
                                                                cTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGa                                                       >  1:6319752/1‑151 (MQ=255)
                                                                 tGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGaa                                                      <  1:4010776/151‑1 (MQ=255)
                                                                 tGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGaa                                                      >  1:967478/1‑151 (MQ=255)
                                                                  ggggCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAAt                                                     >  1:6423674/1‑151 (MQ=255)
                                                                   gggCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATg                                                    >  2:1700847/1‑151 (MQ=255)
                                                                   gggCCTCAGCGAGTGAGGGGCAACCATGCCCGTCACCTGCCTCCGCAAGGCCGGCGTTCGGCCTCATGAAGTCGCAAAGATCAAGATCAAGAACAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATg                                                    <  2:3193494/151‑1 (MQ=14)
                                                                      ccTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGc                                                 >  1:4460648/1‑151 (MQ=255)
                                                                      ccTCAGCGCTTCACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCAGAAGTCGCGAAGATCAGGATCAAGATCAACATCAAGATCAACGGCAACGTCAACGACAACGGCAACAACAACAGCAGGCGAAGGGGc                                                 >  2:6511050/1‑151 (MQ=14)
                                                                       cTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCa                                                >  1:2076116/1‑151 (MQ=255)
                                                                       cTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCa                                                >  2:2707576/1‑151 (MQ=255)
                                                                         cAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACt                                              >  2:1937085/1‑151 (MQ=255)
                                                                         cAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCACGCGAATGGCCACt                                              >  2:5239301/1‑151 (MQ=255)
                                                                            cgcTTGGCGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGcc                                           <  1:5428957/151‑1 (MQ=255)
                                                                             gcTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCa                                          <  1:4828070/151‑1 (MQ=255)
                                                                              cTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAg                                         >  2:3351071/1‑151 (MQ=255)
                                                                               ttGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGa                                        <  1:1385835/151‑1 (MQ=255)
                                                                                ggACGGGCATGGATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGaa                                       <  1:729258/150‑1 (MQ=255)
                                                                                 gACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAAt                                      >  1:5883913/1‑151 (MQ=255)
                                                                                 gACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAAt                                      >  1:5260463/1‑151 (MQ=255)
                                                                                   cGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATca                                    <  2:2847039/151‑1 (MQ=255)
                                                                                    gggCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATcac                                   <  1:845282/151‑1 (MQ=255)
                                                                                    gggCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATcac                                   >  1:4710965/1‑151 (MQ=255)
                                                                                    gggCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATcac                                   >  1:5390438/1‑151 (MQ=255)
                                                                                    gggCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATcac                                   <  2:3090874/151‑1 (MQ=255)
                                                                                        aTCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGtt                               >  1:1353862/1‑151 (MQ=255)
                                                                                         tCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGttt                              >  2:4521966/1‑151 (MQ=255)
                                                                                         tCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGttt                              <  1:1906516/151‑1 (MQ=255)
                                                                                           cATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTtac                            >  2:4704354/1‑151 (MQ=255)
                                                                                            aTGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTtact                           <  2:3471034/151‑1 (MQ=255)
                                                                                            aTGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTtact                           >  1:3803168/1‑151 (MQ=255)
                                                                                            aTGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTtact                           >  2:1039896/1‑151 (MQ=255)
                                                                                              gCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTtactac                         <  1:6226832/151‑1 (MQ=255)
                                                                                              gCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTtactac                         >  2:1359404/1‑151 (MQ=255)
                                                                                               cccGCCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTactact                        <  2:929698/151‑1 (MQ=255)
                                                                                                ccGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAATATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTg                       >  1:1582713/1‑151 (MQ=255)
                                                                                                ccGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTg                       >  1:2879370/1‑151 (MQ=255)
                                                                                                 cGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTgc                      >  1:69863/1‑150 (MQ=255)
                                                                                                 cGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTCTACTACTgg                      >  2:5136269/1‑151 (MQ=255)
                                                                                                    cACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGccc                   >  1:3558280/1‑151 (MQ=255)
                                                                                                       ctgcctccGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGGACTGCCAGAATCACAGTTTACTACTGGCCCTTc                >  2:5631567/4‑151 (MQ=255)
                                                                                                       ctgcctccGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTc                >  1:1682873/4‑151 (MQ=255)
                                                                                                         gcctccGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCag              >  2:4045756/2‑151 (MQ=255)
                                                                                                          cctccGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCagc             >  1:4821317/1‑151 (MQ=255)
                                                                                                             ccGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCagctga          >  2:277165/1‑151 (MQ=255)
                                                                                                             ccGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCagctga          <  2:1505544/151‑1 (MQ=255)
                                                                                                              cGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCagctgag         >  1:752950/1‑151 (MQ=255)
                                                                                                                cAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCagctgagct       >  2:48026/1‑151 (MQ=255)
                                                                                                                 aaGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCagctgagctg      >  2:3681426/1‑151 (MQ=255)
                                                                                                                   ggCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCAGCTGAGCTggg    <  1:3357997/151‑1 (MQ=255)
                                                                                                                   ggCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCAGCTGAGCTggg    <  1:5166213/151‑1 (MQ=255)
                                                                                                                    gCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCAGCTGAGCTgggg   <  1:5986142/151‑1 (MQ=255)
                                                                                                                     ccTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCAGCTGAGCTGGGGt  >  1:4858886/1‑151 (MQ=255)
                                                                                                                        |                                                                                                                                                   
GCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCAGCTGAGCTGGGGT  >  CP009974/4147311‑4147578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑