Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 4,242,144 G→A G443D (GGC→GAC)  RPPX_18895 → aconitate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099744,242,1440GA99.1% 1152.3 / ‑5.8 323G443D (GGC→GAC) RPPX_18895aconitate hydratase
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base A (152/167);  total (153/170)
Fisher's exact test for biased strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 323 total aligned reads displayed.

CGGACGGCGCGGTAATCATCGCTGCCATCACCAGTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGGCTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTGCAACGGTATGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGA  >  CP009974/4241997‑4242291
                                                                                                                                                   |                                                                                                                                                   
cGGACGGCGCGGTAATCATCGCTGCCATCACCAGTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTc                                                                                                                                                  >  1:4494368/1‑151 (MQ=255)
      gcgcGGTAATCATCGCTGCCATCACCAGTTGCACCAACACCAGCACCCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGc                                                                                                                                            <  2:4041043/151‑1 (MQ=255)
      gcgcGGTAATCATCGCTGCCATCACCAGTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGc                                                                                                                                            <  1:2808878/151‑1 (MQ=255)
        gcgGTAATCATCGCTGCCATCACCAGTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCg                                                                                                                                          >  1:1585547/1‑151 (MQ=255)
         cgGTAATCATCGCTGCCATCACCGGTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGt                                                                                                                                         <  2:3037191/151‑1 (MQ=255)
         cgGTAATCATCGCTGCCATCACCAGTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGt                                                                                                                                         <  1:781082/151‑1 (MQ=255)
         cgGTAATCATCGCTGCCATCACCAGTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGGCAAGTCGTCGCTGGCACCCGACTCCAAAGCCGt                                                                                                                                         >  1:3319029/1‑151 (MQ=255)
         cgGTAATCATCGCTGCCATCACCAGTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAAGGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGt                                                                                                                                         <  1:1124590/151‑1 (MQ=255)
          ggTAATCATCGCTGCCATCACCAGTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTg                                                                                                                                        >  1:1813530/1‑151 (MQ=255)
          ggTAATCATCGCTGCCATCACCAGTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTg                                                                                                                                        >  1:4219261/1‑151 (MQ=255)
          ggTAATCATCGCTGCCATCACCAGTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTg                                                                                                                                        >  1:6184970/1‑151 (MQ=255)
           gTAATCATCGCTGCCATCACCAGTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGc                                                                                                                                       >  1:1427680/1‑151 (MQ=255)
             aaTCATCGCTGCCATCACCAGTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAg                                                                                                                                     >  1:3046363/1‑151 (MQ=255)
              atcatcGCTGCCATCACCAGTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGc                                                                                                                                    >  2:4309556/1‑151 (MQ=255)
               tcatcGCTGCCATCACCAGTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCt                                                                                                                                   <  2:5793461/151‑1 (MQ=255)
                 atcGCTGCCATCACCAGTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGt                                                                                                                                 <  2:3059289/151‑1 (MQ=255)
                 atcGCTGCCATCACCAGTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCCGt                                                                                                                                 >  2:4331337/1‑151 (MQ=255)
                   cGCTGCCATCACCAGTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTAc                                                                                                                               >  1:1998149/1‑151 (MQ=255)
                   cGCTGCCATCACCAGTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGCAc                                                                                                                               >  1:3348833/1‑151 (MQ=255)
                       gCCATCACCAGTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTgg                                                                                                                           >  1:5431285/1‑151 (MQ=255)
                       gCCATCACCAGTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTgg                                                                                                                           >  2:3460254/1‑151 (MQ=255)
                       gCCATCACCAGTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTgg                                                                                                                           <  2:645045/151‑1 (MQ=255)
                           tCACCAGTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTggagga                                                                                                                       <  2:5406515/151‑1 (MQ=255)
                             aCCAGTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAg                                                                                                                     <  1:5168501/151‑1 (MQ=255)
                             aCCAGTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAg                                                                                                                     >  2:2898291/1‑151 (MQ=255)
                                 gTTGCACCAACACCAGCAACCCGCGGAACGGGATTGCCGCCGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAgg                                                                                                                 <  2:4995710/151‑1 (MQ=255)
                                 gTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAgg                                                                                                                 <  2:2621046/151‑1 (MQ=255)
                                 gTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAgg                                                                                                                 <  1:2208505/151‑1 (MQ=255)
                                 gTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGAAGCTGTACCTGGAGGAAGCAgg                                                                                                                 <  2:2037885/151‑1 (MQ=255)
                                  ttGCACCAACACCGGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAggg                                                                                                                <  2:5665788/151‑1 (MQ=255)
                                   tGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGc                                                                                                               >  2:2833867/1‑151 (MQ=255)
                                    gCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCCGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGCACGCAGGGCt                                                                                                              >  2:5256348/1‑151 (MQ=255)
                                        cAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGgag                                                                                                          <  2:1090090/151‑1 (MQ=255)
                                          acacCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACGCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCCGGAGaa                                                                                                        >  2:359129/1‑151 (MQ=255)
                                              cAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAg                                                                                                    >  2:5700573/1‑151 (MQ=255)
                                               agcagcCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGc                                                                                                   <  2:4903715/151‑1 (MQ=255)
                                                gCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCt                                                                                                  <  2:2521843/151‑1 (MQ=255)
                                                 cAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTg                                                                                                 <  1:3797073/151‑1 (MQ=255)
                                                 cAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTg                                                                                                 <  2:4546402/151‑1 (MQ=255)
                                                   aCCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGGGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGa                                                                                               <  2:4451879/151‑1 (MQ=255)
                                                       gcgcAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCaa                                                                                           >  1:1179424/1‑151 (MQ=255)
                                                       gcgcAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCaa                                                                                           <  1:2360488/151‑1 (MQ=255)
                                                         gcAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACt                                                                                         >  2:883565/1‑151 (MQ=255)
                                                         aaaaCGTAATTGCAGCAAGCCTGATTGCGAGCAACGCCAACAAGATGGGCCTGACCCGCAAGCCGTAGATCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTATACCTGAAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACt                                                                                         <  2:1260090/149‑1 (MQ=255)
                                                           aaCGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTCCCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTg                                                                                       >  2:3850887/1‑151 (MQ=255)
                                                                ggctGCCGCCGGCCGGGTGGCGTACAAAGCCAGCAAGCCCCGCCCAACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTc                                                                                  <  2:3959772/148‑1 (MQ=255)
                                                                gATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTc                                                                                  <  1:4310909/151‑1 (MQ=255)
                                                                 aTTTCCGCCGTCCTGGTTGCGCGCAATGCCAACATGCTGGGCCTGGCTCGCAAGACGTGGGCCACGTCGTCCCTGGCACCCGACTCCCAGGCCGTGCAGCTGTACCCGGAGGAAGCAGGGCTGGAGAAGGTGCTGGAGCCCCTTGGCTTCg                                                                                 >  2:3164794/1‑151 (MQ=255)
                                                                 aTTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCg                                                                                 <  1:3456006/151‑1 (MQ=255)
                                                                    gCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCa                                                                              <  1:1457608/151‑1 (MQ=255)
                                                                     ccGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGTCCAACTTGGCTTCGGCAt                                                                             >  2:230616/1‑151 (MQ=255)
                                                                      cGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCAtc                                                                            >  1:368335/1‑151 (MQ=255)
                                                                      cGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCAtc                                                                            <  1:1568155/151‑1 (MQ=255)
                                                                        cAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAGGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCAtcgt                                                                          <  2:2056574/151‑1 (MQ=255)
                                                                        cAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCAtcgt                                                                          <  1:2498188/151‑1 (MQ=255)
                                                                         aGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCAtcgtc                                                                         <  1:1018193/151‑1 (MQ=255)
                                                                         aGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCAtcgtc                                                                         <  1:6145015/151‑1 (MQ=255)
                                                                         aGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCAtcgtc                                                                         <  2:1715331/151‑1 (MQ=255)
                                                                           gCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGtcgc                                                                       <  1:3513870/151‑1 (MQ=255)
                                                                            ccTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGtcgcc                                                                      <  2:5930428/151‑1 (MQ=255)
                                                                              tGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGtcgcctt                                                                    >  1:6094915/1‑151 (MQ=255)
                                                                              tGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGtcgcctt                                                                    <  1:5234618/151‑1 (MQ=255)
                                                                               gATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGtcgccttc                                                                   >  1:5603844/1‑151 (MQ=255)
                                                                                 ttGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGtcgccttcgc                                                                 >  1:866348/1‑151 (MQ=255)
                                                                                   gcgcgcAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGtcgccttcgcct                                                               <  1:3967207/151‑1 (MQ=255)
                                                                                    ggcgcAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGGCTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTg                                                              <  2:5836522/150‑1 (MQ=255)
                                                                                    cgcgcAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTg                                                              <  2:5226803/151‑1 (MQ=255)
                                                                                     gcgcAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGc                                                             >  1:584880/1‑151 (MQ=255)
                                                                                     gcgcAATGCCAACAAGCGGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCGGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGc                                                             <  2:3786922/151‑1 (MQ=255)
                                                                                      cgcAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGca                                                            >  1:2065103/1‑151 (MQ=255)
                                                                                             ccAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTg                                                     <  2:1143595/151‑1 (MQ=255)
                                                                                                 caaGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTGCAAc                                                 <  1:2533595/151‑1 (MQ=255)
                                                                                                      tGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCTGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTGCAACGGTAt                                            <  1:1871578/151‑1 (MQ=255)
                                                                                                      tGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTGCAACGGTAt                                            >  1:6280856/1‑151 (MQ=255)
                                                                                                      tGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTGCAACGGTAt                                            <  2:1971471/151‑1 (MQ=255)
                                                                                                       gggCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTGCAACGGTATg                                           <  2:3388760/151‑1 (MQ=255)
                                                                                                         gCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTGCAACGGTATGTc                                         >  1:2862340/1‑151 (MQ=255)
                                                                                                         gCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTGCAACGGTATGTc                                         <  1:3198660/151‑1 (MQ=255)
                                                                                                             gACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTGCAACGGTATGTCCGgc                                     <  2:2133127/151‑1 (MQ=255)
                                                                                                                  gCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTGCAACGGTATGTCCGGCGCCCt                                >  1:6493326/1‑151 (MQ=255)
                                                                                                                  gCAAGCCGGGCGTCAGGCCGTCGCTCGCCCCCGGGCGCTAGGCCGTGCAGGGGGAGCTGGAGGAAGCAGGACTGGAGCAGGGGCTGGAGGAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCCCCTGCAACGGGATGTCCGGCGCCCt                                <  2:5891193/151‑1 (MQ=255)
                                                                                                                     aGCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTGCAACGGTATGTCCGGCGCCCTGGa                             >  2:1607714/1‑151 (MQ=255)
                                                                                                                      gCCGTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTGCAACGGTATGTCCGGCGCCCTGGAc                            >  2:2217352/1‑151 (MQ=255)
                                                                                                                         gTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTGCAACGGTATGTCCGGCGCCCTGGACCCg                         <  2:2353661/151‑1 (MQ=255)
                                                                                                                         gTGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTGCAACGGTATGTCCGGCGCCCTGGACCCg                         >  2:1877011/1‑151 (MQ=255)
                                                                                                                         gTGGGTCAAGTCGGCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTGCAACGGTATGTCCGGCGCCCTGGACCCg                         <  2:3315257/151‑1 (MQ=255)
                                                                                                                          tGGGTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTGCAACGGTATGTCCGGCGCCCTGGACCCgg                        >  2:6344090/1‑151 (MQ=255)
                                                                                                                            ggTCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTGCAACGGTATGTCCGGCGCCCTGGACCCGGTg                      >  1:4825810/1‑151 (MQ=255)
                                                                                                                              tCAAGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTGCAACGGTATGTCCGGCGCCCTGGACCTGGTGAt                    >  2:1862993/1‑151 (MQ=255)
                                                                                                                                aaGTCGTCGCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTGCAACGGTATGTCCGGCGCCCTGGACCCGGTGATcc                  <  1:999510/151‑1 (MQ=255)
                                                                                                                                    cgtcgCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTGCAACGGTATGTCCGGCGCCCTGGACCCGGTGATCCAGCa              <  1:2922729/151‑1 (MQ=255)
                                                                                                                                       cgCTGGCACCGGACTCGCGGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAAATTGGCTTAGGCATCGTAGCCTTCGCCTGCACCACCTGCAACGGTATGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGa           <  2:664083/151‑1 (MQ=255)
                                                                                                                                       cgCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTGCAACGGTATGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGa           <  2:4738353/151‑1 (MQ=255)
                                                                                                                                        gCTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTGCAACGGTATGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGaa          <  1:652163/151‑1 (MQ=255)
                                                                                                                                         cTGGCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTGCAACGGTATGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGaaa         <  1:3620659/151‑1 (MQ=255)
                                                                                                                                          gggCACCGGACTGCAAGGCCGGGCAGGTGTAGCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCGTCGTCGCCTTCGCCTGCACCACCTGCAACGGTATGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAat        <  2:6202061/150‑1 (MQ=255)
                                                                                                                                           ggCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTGCAACGGTATGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAatc       <  1:1210053/151‑1 (MQ=255)
                                                                                                                                            gCACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTGCAACGGTATGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAatca      <  1:2614535/151‑1 (MQ=255)
                                                                                                                                             cACCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTGCAACGGTATGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAatcat     <  1:433446/151‑1 (MQ=255)
                                                                                                                                              aCCCGACTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTGCAACGGTATGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAatcatc    >  1:1312992/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGGACGGCGCGGTAATCATCGCTGCCATCACCAGTTGCACCAACACCAGCAACCCGCGCAACGTGATTGCCGCAGGCCTGATTGCGCGCAATGCCAACAAGCTGGGCCTGACCCGCAAGCCGTGGGTCAAGTCGTCGCTGGCACCCGGCTCCAAGGCCGTGCAGCTGTACCTGGAGGAAGCAGGGCTGGAGAAGGAGCTGGAGCAACTTGGCTTCGGCATCGTCGCCTTCGCCTGCACCACCTGCAACGGTATGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGA  >  CP009974/4241997‑4242291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑