Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 542,104 C→G intergenic (‑365/+164) argD ← / ← RPPX_02300 acetylornithine aminotransferase/AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009974542,1040CG88.5% 81.3 / 9.3 52intergenic (‑365/+164)argD/RPPX_02300acetylornithine aminotransferase/AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/6);  new base G (4/42);  total (4/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTCCCTCCTGTGCCTGGCGCGCGCGACCGCCTTGTTTGTACGACGCGATATCGAGGCATGCGCCAAGGCGTTCGCGCGCAAATGCCACAGGCATAACTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATTTGCGCCCCAACATCAACCTCAAGGCATAC  >  CP009974/541956‑542246
                                                                                                                                                    |                                                                                                                                              
aGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGa                                                                                                                                              <  1:262799/151‑1 (MQ=18)
 ggggACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGat                                                                                                                                             <  2:2749944/151‑1 (MQ=18)
   ggACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGatat                                                                                                                                           <  1:5427732/151‑1 (MQ=18)
   ggACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGatat                                                                                                                                           <  1:5345295/151‑1 (MQ=18)
   ggACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGatat                                                                                                                                           <  2:3083254/151‑1 (MQ=18)
     aCAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCg                                                                                                                                         >  2:2902108/1‑151 (MQ=17)
       aGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAg                                                                                                                                       <  1:5259976/151‑1 (MQ=17)
        ggATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAgg                                                                                                                                      <  1:1809061/151‑1 (MQ=17)
           tGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggtgt                                                                                                                                   <  2:3281194/151‑4 (MQ=17)
           tGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggtgt                                                                                                                                   <  1:5240683/151‑4 (MQ=17)
           tGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggtgt                                                                                                                                   <  2:3280355/151‑4 (MQ=17)
           tGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggtgt                                                                                                                                   <  1:4394828/151‑4 (MQ=17)
           ggTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggtgt                                                                                                                                   <  2:3346110/150‑4 (MQ=17)
            gTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggtgtg                                                                                                                                  <  1:1220467/151‑5 (MQ=17)
            gTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggtgtg                                                                                                                                  <  1:3777902/151‑5 (MQ=17)
              aCGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggtgtgcg                                                                                                                                <  1:3536528/151‑7 (MQ=17)
              aCGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggtgtgcg                                                                                                                                <  1:2686686/151‑7 (MQ=17)
                 gCGATGTTGATGTGTTGGCGGGGAGATCGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCa                                                                                                                             <  1:2712364/151‑1 (MQ=14)
                   gATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAg                                                                                                                           <  1:688319/151‑1 (MQ=14)
                   gATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAg                                                                                                                           <  1:2833822/151‑1 (MQ=14)
                   gATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAg                                                                                                                           <  1:6289258/151‑1 (MQ=14)
                   gATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAg                                                                                                                           <  1:5617892/151‑1 (MQ=14)
                   gATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAg                                                                                                                           <  1:536255/151‑1 (MQ=14)
                    gtGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAgg                                                                                                                          <  2:464662/150‑1 (MQ=14)
                    aTGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAgg                                                                                                                          <  1:5530348/151‑1 (MQ=14)
                     ggTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGc                                                                                                                         <  2:808804/150‑1 (MQ=14)
                       ttGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGt                                                                                                                       <  1:4516514/151‑1 (MQ=14)
                       ttGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGt                                                                                                                       <  1:223373/151‑1 (MQ=14)
                        tGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGtt                                                                                                                      <  2:4202900/151‑1 (MQ=14)
                        ggATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGtt                                                                                                                      <  2:4215284/150‑1 (MQ=14)
                         gATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTCCGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTc                                                                                                                     <  1:6774/151‑1 (MQ=14)
                         gATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTc                                                                                                                     <  1:6051069/151‑1 (MQ=14)
                         gATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTc                                                                                                                     <  2:4474404/151‑1 (MQ=14)
                           tgtgTCGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTcgc                                                                                                                   <  1:3231737/151‑1 (MQ=11)
                            gtgtTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTcgcg                                                                                                                  <  1:3654188/151‑1 (MQ=11)
                             tgtTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTcgcgc                                                                                                                 <  1:2821053/151‑1 (MQ=11)
                                tGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCa                                                                                                              <  1:1943197/151‑1 (MQ=11)
                                  gCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCaaa                                                                                                            <  1:3725168/151‑1 (MQ=11)
                                   cGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCAAAt                                                                                                           >  2:5617581/1‑151 (MQ=11)
                                     tagtgaTCGAGCGCCGCCCGAGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTCCCTCCTGCACCAGTCGCGCGCGACCGCCTTGTTTGTACGACGCGATATCGAGACATGCGCCAAGGCGGTCGCGCGCAAAtcc                                                                                                         <  2:2039311/147‑3 (MQ=1)
                                     gggAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCAAATGc                                                                                                         <  1:3578539/151‑1 (MQ=11)
                                      ggAGCTCGAGGGCCGCCCGGGCGGCGCTTCGCGGGTGAGCCCGCTCCTACGCGTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCAAATGcc                                                                                                        <  2:6244409/151‑1 (MQ=11)
                                           gcgggcgcCGCCCGCGCGGCGCTTCGCGGGTAAGCCCGCTCCTACGTTTGTTTCGGGCCAGTCCATCCTGTGCCTGGCGCGCGCGACCGCCTTGTTTGTACGACGCGATATCGAGGCATGCACCAAGGCGTTCGCGCGCAAATCCCACAgg                                                                                                   <  2:5775045/147‑1 (MQ=1)
                                                                         gaagcccGCGCCGGCGCTTGGTTCGGGCGAGTCCCGCCTGCGGCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGGGTGCGCCAAGGCGTTCGCGCGCAAATGCCACAGGCATAACTGGCCCGAAACAAACGTAGGAGca                                                                     <  2:5083287/147‑2 (MQ=1)
                                                                          aaccccGCTCCTCCGTGTGTTTCGGGCCAGTCCCTCCTGTGCCTGGCGCGCGCGACCGCCTTGTTGGTACGACGCGATATCGAGACAGGCACCAAGGCGTTCGCGCGCGAATCCCACAGGCATAATTGGCCCGAAACAAACGTAGGAGCgg                                                                    <  2:3798679/148‑1 (MQ=1)
                                                                          aaccccGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCAAATGCCACAGGCATAACTGGCCCGAAACAAACGTAGGAGcag                                                                    <  1:4786093/148‑3 (MQ=1)
                                                                           aatccGCTCCTACGTTTGTTTCGGGCCAGTCCATCCTGTGCCTGGCGCGCGCGACCGCCTTGTTTGTACGACGCGTTATCGAGGCATGCACCAAGGCGTTCGCGCGCAAATCCCACAGGCATAATTGGCCTGTTACAATCGTAGGTGCggg                                                                   <  2:5147399/148‑1 (MQ=1)
                                                                                gccccgaCGTTTGTGCCGGGCCACCCCCCCCTGCGCCAGTCGCGCGCGACCGCCTTGTTTGTACGACGCCACATCGAGACATGCGCCAAGGCGGTCGCGCGCAAATCCCACAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTAc                                                              <  1:1663145/145‑1 (MQ=1)
                                                                                   cccaCGTTTTTTCCGGGCCAGCCCCTCCTGTGCCTGGCGGGCGCGCCCGCCTTGTTGGTACGACGCGATATCGAGACAGGCACCAAGGCGTTCGCGCGCGAATCCCACAGGCATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCcg                                                           <  1:5799229/148‑1 (MQ=1)
                                                                                       cgcttgtttCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCAAATGCCACAGGCATAACTGGCCCGAAACAAACGTAGGAGCAGGTTTACCCGCGaa                                                       <  2:4757867/148‑1 (MQ=1)
                                                                                       cgcttgtttCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCAAATGCCACAGGCATAACTGGCCCGAAACAAACGTAGGAGCAGGTTTACCCGCGaa                                                       >  1:2408678/4‑151 (MQ=1)
                                                                                                                                            gttcgacgGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCAAATGCCACAGGCATAACTGGCCCGACACAAACGTAGGAGCAGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATTTGCGCCCCAACATCAACCTCAAGGCATac  >  2:2201793/4‑151 (MQ=1)
                                                                                                                                                    |                                                                                                                                              
AGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTCCCTCCTGTGCCTGGCGCGCGCGACCGCCTTGTTTGTACGACGCGATATCGAGGCATGCGCCAAGGCGTTCGCGCGCAAATGCCACAGGCATAACTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATTTGCGCCCCAACATCAACCTCAAGGCATAC  >  CP009974/541956‑542246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑