Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 4,384,773 A→G G174G (GGA→GGG)  RPPX_19530 → nucleoside hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099744,384,7730AG99.0% 1048.0 / 3.4 294coding (522/1002 nt)RPPX_19530nucleoside hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (149/142);  minor base . (2/1);  total (151/143)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 302 total aligned reads displayed.

TGCCGTGCAATACCTGGTCGATACCCTCGGCGCAGCCAAACCGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGAGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGCTGACCTACCTGCCGCTGGATGTCACGCACAAGCTGCTGACCAGTAACGCC  >  CP009974/4384623‑4384920
                                                                                                                                                       |                                                                                                                                                   
tGCCGTGCAATACCTGGTCGATACCCTCGGCGCAGCCAAACCGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGa                                                                                                                                                     >  1:5828153/1‑151 (MQ=255)
  ccGTGCAATACCTGGTCGATACCCTCGGCGCAGCCAAACCGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGgc                                                                                                                                                   <  1:1819646/151‑1 (MQ=255)
    gTGCAATCCCTGGTCGCTACCCGCGGCGCAGCCAAACCGCACAGCAGCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGgcgc                                                                                                                                                 <  2:4438490/151‑1 (MQ=255)
     tGCAATACCTGGTCGATACCCTCGGCGCAGCCAAACCGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCGT‑GGGGGGGGCGCa                                                                                                                                                >  1:6294878/1‑151 (MQ=255)
       cAATACCTGGTCGATACCCTCGCCGCAGCCAAACCGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCAtt                                                                                                                                              <  2:1095177/151‑1 (MQ=255)
         aTACCTGGTCGATACCCTCGGCGCAGCCAAACCGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTc                                                                                                                                            <  2:2556419/151‑1 (MQ=255)
         aTACCTGGTCGATACCCTCGGCGCAGCCAAACCGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTc                                                                                                                                            <  2:2086152/151‑1 (MQ=255)
           aCCTGGTCGATACCCTCGGCGCAGCCAAACCGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCaa                                                                                                                                          >  2:1535632/1‑151 (MQ=255)
              tGGTCGATACCCTCGGCGCAGCCAAACCGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGGGGCGGTCAT‑GGGGGGGGCGCATTTCAAcgg                                                                                                                                       >  2:3386537/1‑151 (MQ=255)
              tGGGCGAGACCCTCGGCGCAGCCAAACCGCACGGCATCACCGTCGCCAGGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAAcgg                                                                                                                                       <  2:3412967/151‑1 (MQ=255)
              tGGGCGAGACCCTCGGCGCAGCCAAACCGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAAcgg                                                                                                                                       <  2:2960283/151‑1 (MQ=255)
               ggTCGATACCCTCGGCGCAGCCAAACCGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAAcggc                                                                                                                                      <  2:3241167/151‑1 (MQ=255)
                gTCGATACCCTCGGCGCAGCCAAACCGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAAcggcg                                                                                                                                     <  2:1731666/151‑1 (MQ=255)
                gTCGATACCCTCGGCGCAGCCAAACCGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAAcggcg                                                                                                                                     <  2:6336811/151‑1 (MQ=255)
                gTCGATACCCTCGGCGCAGCCAAACCGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAAcggcg                                                                                                                                     >  1:3644814/1‑151 (MQ=255)
                 tCGATACCCTCGGCGCAGCCAAACCGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAAcggcgg                                                                                                                                    >  2:2491090/1‑151 (MQ=255)
                  cGATACCCTCGGCGCAGCCAAACCGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACggcggc                                                                                                                                   >  1:4103623/1‑151 (MQ=255)
                   gATACCCTCGGCGCAGCCAAACCGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCa                                                                                                                                  >  1:4786563/1‑151 (MQ=255)
                   gATACCCTCGGCGCAGCCAAACCGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCa                                                                                                                                  <  1:6512529/151‑1 (MQ=255)
                    aTACCCTCGGCGCAGCCAAACCGCACAGCATCCCCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCaa                                                                                                                                 <  1:785488/151‑1 (MQ=255)
                         cTCGGCGCAGCCAAACCGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCCGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATcc                                                                                                                            >  2:6173017/1‑150 (MQ=255)
                         cTCGGCGCAGCCAAACCGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCa                                                                                                                            >  1:3024710/1‑151 (MQ=255)
                         cTCGGCGCAGCCAAACCGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCa                                                                                                                            <  2:3967870/151‑1 (MQ=255)
                         cTCGGCGCAGCCAAACCGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCa                                                                                                                            >  1:6137744/1‑151 (MQ=255)
                          tCGGCGCAGCCAAACCGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCAc                                                                                                                           >  2:1357811/1‑151 (MQ=255)
                              cgcAGCCAAACCGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCa                                                                                                                       >  1:6150668/1‑151 (MQ=255)
                              cgcAGCCAAACCGCACAGCATCACCGTCGCCATGCGCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCa                                                                                                                       <  2:5504969/151‑1 (MQ=255)
                                      aaCCGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGa                                                                                                               <  1:4928238/151‑1 (MQ=255)
                                        ccGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCATGGGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAg                                                                                                              <  1:35030/151‑1 (MQ=255)
                                        ccGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAgg                                                                                                             >  2:4052591/1‑150 (MQ=255)
                                        ccGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGt                                                                                                             <  2:860638/151‑1 (MQ=255)
                                         cGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGtt                                                                                                            >  1:636389/1‑151 (MQ=255)
                                          gCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTc                                                                                                           >  1:2312270/1‑151 (MQ=255)
                                              aGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAAcc                                                                                                       >  2:2808035/1‑151 (MQ=255)
                                                cATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGTGCATCGCCAAAGGCATCAAAGAGGTGGCGGTCAT‑GGGGGGGGCGCATTTCTACGGCGGCAACAACACTGCAGTGGCGGAGTTCAACgtc                                                                                                     >  1:2851122/1‑151 (MQ=255)
                                                 aTCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGCTCAACctct                                                                                                    >  2:3175738/1‑151 (MQ=255)
                                                  tCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTa                                                                                                   >  1:820105/1‑151 (MQ=255)
                                                  tCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTa                                                                                                   >  1:758135/1‑151 (MQ=255)
                                                  tCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTa                                                                                                   >  2:833405/1‑151 (MQ=255)
                                                      cGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGcc                                                                                               >  1:698690/1‑151 (MQ=255)
                                                      cGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGcc                                                                                               >  2:1620153/1‑151 (MQ=255)
                                                       gTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCg                                                                                              >  2:5326180/1‑151 (MQ=255)
                                                        tCGCCCTGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGa                                                                                             <  1:2015900/151‑1 (MQ=255)
                                                        tCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAGGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGa                                                                                             <  1:1173517/151‑1 (MQ=255)
                                                          gCCATGCGCGGCCCGCAGGCCGACCGGGCCGTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGAcc                                                                                           <  2:1992427/151‑1 (MQ=255)
                                                           ccATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGAccc                                                                                          <  1:5043718/151‑1 (MQ=255)
                                                               gCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCAc                                                                                      <  1:1310053/151‑1 (MQ=255)
                                                               gCTCGGCCCGCACACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCAc                                                                                      >  2:4613542/1‑151 (MQ=255)
                                                                cTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCAcg                                                                                     <  1:3139335/151‑1 (MQ=255)
                                                                cTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCAcc                                                                                     >  2:5865242/1‑150 (MQ=255)
                                                                  cGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCAcgcc                                                                                   >  2:2392497/1‑151 (MQ=255)
                                                                  cGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCAcgcc                                                                                   <  2:483849/151‑1 (MQ=255)
                                                                   ggCCCGCAGACCAGCCGGGCCGTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCAcgccg                                                                                  <  2:2706207/151‑1 (MQ=255)
                                                                   ggCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAAGATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCAcgccg                                                                                  >  2:5723133/1‑151 (MQ=255)
                                                                   ggCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGGGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCAcgccg                                                                                  >  2:1410090/1‑151 (MQ=255)
                                                                     cccGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACgccgcc                                                                                <  1:1853152/151‑1 (MQ=255)
                                                                      ccGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGccgccg                                                                               >  1:5452872/1‑151 (MQ=255)
                                                                      ccGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGccgccg                                                                               >  2:1496241/1‑151 (MQ=255)
                                                                            aCCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAgtgg                                                                         >  2:1826920/1‑151 (MQ=255)
                                                                            aCCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAgtgg                                                                         >  1:1558987/1‑151 (MQ=255)
                                                                                aCCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGGTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGgcgcc                                                                     >  2:3339722/1‑150 (MQ=255)
                                                                                  ctggccctggcGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTgg                                                                   <  1:2919929/151‑1 (MQ=255)
                                                                                     gccctggcGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCa                                                                <  2:566631/151‑1 (MQ=255)
                                                                                       cctggcGCTGATCCAGCGCCCGCGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATGTCAACGCCGGCACCGTCACCCCCGTGGCGGCGCTCAACCTCTCCGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAgc                                                              <  2:4241620/151‑1 (MQ=255)
                                                                                       cctggcGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCGAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAgc                                                              <  2:5569660/151‑1 (MQ=255)
                                                                                       cctggcGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAgc                                                              >  1:5608743/1‑151 (MQ=255)
                                                                                        ctggcGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAgcg                                                             <  2:3780881/151‑1 (MQ=255)
                                                                                          ggcGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGGGGTGCTGGCCAgcggc                                                           >  1:530209/1‑151 (MQ=255)
                                                                                              cTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGc                                                       >  1:3682395/1‑151 (MQ=255)
                                                                                               tGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGCCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCa                                                      <  2:5043808/151‑1 (MQ=255)
                                                                                                gATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAg                                                     <  2:6538243/151‑1 (MQ=255)
                                                                                                 aTCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGc                                                    <  1:4090145/151‑1 (MQ=255)
                                                                                                   ccAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGCTg                                                  >  1:1734897/1‑151 (MQ=255)
                                                                                                    cAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGCTGa                                                 <  1:5245559/151‑1 (MQ=255)
                                                                                                        gcCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGCTGaccta                                             >  1:2582983/1‑151 (MQ=255)
                                                                                                        gcCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGCTGaccta                                             >  1:5923204/1‑151 (MQ=255)
                                                                                                         cccGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGCTGacctac                                            >  1:3792699/1‑151 (MQ=255)
                                                                                                           cGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGCTGacctaccc                                          >  2:1180709/1‑150 (MQ=255)
                                                                                                            gAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGCTGACCTACCTg                                         >  1:2732644/1‑151 (MQ=255)
                                                                                                             aGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGCTGACCTACCTGc                                        >  1:4249850/1‑151 (MQ=255)
                                                                                                                  cGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGCTGACCTACCTGCCGCTg                                   >  2:5010005/1‑151 (MQ=255)
                                                                                                                  cGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGCTGACCTACCTGCCGCTg                                   <  2:5152186/151‑1 (MQ=255)
                                                                                                                     cAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGCTGACCTACCTGCCGCTGGAt                                <  1:1763591/151‑1 (MQ=255)
                                                                                                                     cAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGACGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGCTGACCTACCTGCCGCTGGAt                                <  1:345641/151‑1 (MQ=255)
                                                                                                                      aaaGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCGCCCCAGTGGCGGAGTTCAACCGCTACGCCGACCCGCACGCCGCCGAAGCGGTGCTGGCCAGCGGCGTGCAGCTGACCTACCTGCCGCTGGATg                               <  2:412723/151‑1 (MQ=255)
                                                                                                                      aaaGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGCTGACCTACCTGCCGCTGGATg                               >  2:611858/1‑151 (MQ=255)
                                                                                                                       aggggaTCAGAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGCTGACCTACCTGCCGCTGGATGt                              <  2:2168381/146‑1 (MQ=255)
                                                                                                                       aaGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGCTGACCTACCTGCCGCTGGATGt                              >  2:1142337/1‑151 (MQ=255)
                                                                                                                            aTCAAAGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGCTGACCTACCTGCCGCTGGATGTCACGc                         >  1:1877074/1‑151 (MQ=255)
                                                                                                                              cAAAGAGGGGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGCTGACCTACCTGCCGCTGGATGTCACGcac                       <  2:3646656/151‑1 (MQ=255)
                                                                                                                               aaaGAGCTGGTGGTCATGGGGGGGGGCGCACTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAACTGGAGCTGCCCAGCGGCGTGCAGCTGACCTACCTGCCGCCGGATGCCACGcac                       >  2:6250919/1‑151 (MQ=255)
                                                                                                                                aaGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGCTGACCTACCTGCCGCTGGATGTCACGCACaa                     <  1:2265865/151‑1 (MQ=255)
                                                                                                                                aaGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGCTGACCTACCTGCCGCTGGATGTCACGCACaa                     <  1:1652919/151‑1 (MQ=255)
                                                                                                                                aaGAGGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGCTGACCTACCTGCCGCTGGATGTCACGCACaa                     <  1:4365702/151‑1 (MQ=255)
                                                                                                                                 agagGTGGTGGTCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGCTGACCTACCTGCCGCTGGATGTCACGCACAAg                    >  2:4883181/1‑151 (MQ=255)
                                                                                                                                    ggtggtggtCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGCTGACCTACCTGCCGCTGGATGTCACGCACAAgctg                 >  1:1055619/1‑151 (MQ=255)
                                                                                                                                      gggtggtCAG‑GGGGGAGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGAAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGCTGACCTACCTGCCGCTGGATGTCACGCACAAgctgct               <  2:700809/150‑1 (MQ=255)
                                                                                                                                            tCAT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGCTGACCTACCTGCCGCTGGATGTCACGCACAAGCTGCTGACCAg         >  2:3576398/1‑151 (MQ=255)
                                                                                                                                              aT‑GGGGGGGGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGCTGACCTACCTGCCGCTGGATGTCACGCACAAGCTGCTGACCAGTa       <  1:4613542/151‑1 (MQ=255)
                                                                                                                                                  ggggggGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGCTGACCTACCTGCCGCTGGATGTCACGCACAAGCTGCTGACCAGTAAcc    >  1:1419953/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                   
TGCCGTGCAATACCTGGTCGATACCCTCGGCGCAGCCAAACCGCACAGCATCACCGTCGCCATGCTCGGCCCGCAGACCAACCTGGCCCTGGCGCTGATCCAGCGCCCGAGCATCGCCAAAGGCATCAAAGAGGTGGTGGTCAT‑GGGGGGAGCGCATTTCAACGGCGGCAACATCACCCCAGTGGCGGAGTTCAACCTCTACGCCGACCCGCACGCCGCCGAAGTGGTGCTGGCCAGCGGCGTGCAGCTGACCTACCTGCCGCTGGATGTCACGCACAAGCTGCTGACCAGTAACGCC  >  CP009974/4384623‑4384920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑