Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	4,537,108	A→G	Y105Y (TAT→TAC)	RPPX_20270 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	4,537,108	0	A	G	100.0%	297.8 / NA	94	Y105Y (TAT→TAC)	RPPX_20270	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (59/35);  total (59/35)

TGGCGCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTG  >  CP009974/4536961‑4537159                                                                                                                                                    |                                                    tggcgCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAg                                                  <  1:6388461/151‑1 (MQ=255)  ggcgCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGt                                                 >  2:1603567/1‑151 (MQ=255)  ggcgCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGt                                                 <  2:386974/151‑1 (MQ=255)   gcgcTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTg                                                >  2:3324275/1‑151 (MQ=255)    cgcTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTgg                                               >  1:6099255/1‑151 (MQ=255)    cgcTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTgg                                               >  1:4178720/1‑151 (MQ=255)     gctgctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGgc                                              >  2:4416064/1‑151 (MQ=255)      ctgctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGgcg                                             >  1:3624603/1‑151 (MQ=255)       tgctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGgcgc                                            >  2:2767117/1‑151 (MQ=255)       tgctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGgcgc                                            >  2:305798/1‑151 (MQ=255)        gctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACATCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGcc                                           >  1:1961802/1‑151 (MQ=255)        gctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCTCGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGcc                                           >  1:2181033/1‑151 (MQ=255)        gctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGcc                                           >  1:2180964/1‑151 (MQ=255)        gctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGcc                                           >  1:1963893/1‑151 (MQ=255)         ctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCa                                          >  1:6027716/1‑151 (MQ=255)         ctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCa                                          >  2:1882683/1‑151 (MQ=255)         ctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCa                                          >  1:3705077/1‑151 (MQ=255)         cgcGGACGTTGAACGGGCGGCAGATGCCATACCAGCAGAGGGCGACGGGCAGGGTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCa                                          <  2:5581456/149‑1 (MQ=255)          tCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGTGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTTGCGCCac                                         >  1:944121/1‑150 (MQ=255)          tCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAg                                         >  1:1321407/1‑151 (MQ=255)          tCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAg                                         <  1:306798/151‑1 (MQ=255)          tCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAg                                         <  1:2643542/151‑1 (MQ=255)           cGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAgg                                        <  1:4760620/151‑1 (MQ=255)            ggACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGc                                       >  1:532067/1‑151 (MQ=255)            ggACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGc                                       >  2:3282197/1‑151 (MQ=255)            ggACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGc                                       >  1:3232876/1‑151 (MQ=255)              aCGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCTGGTTCGCCAGGGGGCGTTGTGGCGCCAGGCtt                                     >  2:3530194/1‑151 (MQ=255)              aCGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCtt                                     >  2:5323806/1‑151 (MQ=255)              aCGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCtt                                     >  1:3948001/1‑151 (MQ=255)              aCGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCtt                                     >  2:4484851/1‑151 (MQ=255)              aCGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCtt                                     <  1:3324275/151‑1 (MQ=255)               cGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTc                                    <  2:6380046/151‑1 (MQ=255)               cGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTc                                    >  1:697124/1‑151 (MQ=255)               cGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTc                                    <  1:5921442/151‑1 (MQ=255)                gTTGCACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCt                                   >  2:5378744/1‑151 (MQ=255)                gTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCt                                   <  1:212838/151‑1 (MQ=255)                  tGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCTTGCATTTCACCGGGCTCGCCCGTTCGTCAGGGGGCGTCGCGGCGGCAGCCTTCTGc                                 >  2:1712980/1‑151 (MQ=17)                  tGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGc                                 >  2:4979339/1‑151 (MQ=255)                  tGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGc                                 >  2:1712997/1‑151 (MQ=255)                  tGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGc                                 >  2:1243641/1‑151 (MQ=255)                    aaCAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCg                               <  2:3690942/151‑1 (MQ=255)                     aCAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCgg                              >  1:5444375/1‑151 (MQ=255)                       aGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCgggg                            <  2:5970663/151‑1 (MQ=255)                         gcggcgGATGCCATGCCAGCAGAGGGCGGCGGTCAGGTTGTAGGGGAACAGCACGGCCGAGAACGGCAATTGCCGGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGtg                          <  2:1329454/151‑2 (MQ=14)                         gcggcAGCTGCCAGAGCAGCGGGGGGCGACGGGCAGGTTGTAGGGGGACAGCACGGCGCAGAACGGCAATTGCCAGTGCTTTTTGCCCTGCATGTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGGTTCTGCCcggggg                          <  2:5796243/151‑3 (MQ=11)                          cggcAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGtga                         >  1:4877492/1‑149 (MQ=255)                          cggcAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGtga                         <  1:2223176/151‑3 (MQ=255)                          cggcAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGtga                         >  1:4384355/1‑149 (MQ=255)                           ggcAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGTGGTGAg                        >  2:538085/1‑151 (MQ=255)                           ggcAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAg                        <  1:6298794/151‑1 (MQ=255)                           ggcAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAg                        >  2:3858373/1‑151 (MQ=255)                            gcAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCt                                      <  2:2900818/136‑1 (MQ=255)                            gcAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCt                                      >  1:2900818/1‑136 (MQ=255)                            gcAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAgg                       <  2:643669/151‑1 (MQ=255)                            gcAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAgg                       <  1:3079513/151‑1 (MQ=255)                             cAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGc                      <  1:5139082/151‑1 (MQ=255)                             cAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGc                      >  2:4181657/1‑151 (MQ=255)                             cAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGc                      >  2:2377390/1‑151 (MQ=255)                             cAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGc                      <  1:3307879/151‑1 (MQ=255)                              aGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCa                     <  2:5519146/151‑1 (MQ=255)                              aGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCa                     <  2:390495/151‑1 (MQ=255)                              aGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCa                     <  1:2553206/151‑1 (MQ=255)                               gATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAg                    >  2:1042791/1‑151 (MQ=255)                               gATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAg                    >  1:3810490/1‑151 (MQ=255)                               gATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAg                    >  2:1647394/1‑151 (MQ=255)                                aTGCGATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGa                   >  2:5382319/1‑151 (MQ=255)                                aTGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGa                   >  1:306132/1‑151 (MQ=255)                                aTGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGa                   >  2:169197/1‑151 (MQ=255)                                 tGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGCACAGCGCGGCCCAGGACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGAt                  <  2:1785304/151‑1 (MQ=255)                                 tGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGAt                  <  2:1786368/151‑1 (MQ=255)                                 tGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGAt                  <  1:1770845/151‑1 (MQ=255)                                  gCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATg                 >  1:4896614/1‑151 (MQ=255)                                  gCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATg                 >  1:4896404/1‑151 (MQ=255)                                  gCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATg                 <  2:2857561/151‑1 (MQ=255)                                  gCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATg                 >  2:2039982/1‑151 (MQ=255)                                    cATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGc               >  1:6074279/1‑151 (MQ=255)                                    cATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGc               <  1:3642935/151‑1 (MQ=25)                                      tACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGt             >  1:6050660/1‑151 (MQ=255)                                      tACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGACGt             >  2:1765477/1‑151 (MQ=255)                                       aCCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTg            <  1:4139962/151‑1 (MQ=19)                                       aCCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTg            >  1:3205579/1‑151 (MQ=21)                                        ccAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGc           <  1:78710/151‑1 (MQ=255)                                         cagcagAGGGCGACGGTCAGGTTGCAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCCTCTGCCGGGGTGAGGCAGATCGCGTGcc          >  2:1829986/1‑150 (MQ=11)                                          agcagAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTGACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGGGCCAGCCTTCTGCCGGGGTGGGGCAGAGCGCGTGccc         >  2:5113493/1‑149 (MQ=11)                                          agcagAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAg         <  1:1748696/151‑1 (MQ=16)                                            cagAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTg       <  2:2048085/151‑1 (MQ=12)                                              gagGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGc     >  2:2251095/1‑151 (MQ=14)                                              gagGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGc     >  2:2250714/1‑151 (MQ=9)                                               agGGCGCCGGTCAGGTCGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTGACCGGGCCCGCCTGGCCCCCATGGGGCGTAGCGGCCCCAGTCTTCCGCCGGGAGGCGGCAGCTGCCGCGCAGTgccg    >  2:3745956/1‑148 (MQ=2)                                               agGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCt    >  1:5713699/1‑151 (MQ=14)                                               agGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCt    >  2:2233030/1‑151 (MQ=14)                                                gggCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCtt   <  1:381167/151‑1 (MQ=9)                                                gggCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCtt   >  1:6469902/1‑151 (MQ=14)                                                 ggCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCTtg  <  1:3475672/151‑1 (MQ=2)                                                                                                                                                    |                                                    TGGCGCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTG  >  CP009974/4536961‑4537159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑