BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	4,540,460	C→T	R458R (CGG→CGA)	RPPX_20280 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	4,540,460	0	C	T	99.3%	988.9 / NA	303	R458R (CGG→CGA)	RPPX_20280	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (149/151); total (151/152)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 303 total aligned reads displayed.

CCCCGCCAATCCCTCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAACGCCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAGGGGTTATCGATG  >  CP009974/4540313‑4540604

  ccGCCAATCCCTCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTc                                                                                                                                             <  1:6247480/151‑1 (MQ=255)

   cGCCAATCCCTCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCa                                                                                                                                            <  1:3313129/151‑1 (MQ=255)

    gCCAATCCCTCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTCGAAGCGTCGGTCAt                                                                                                                                           >  2:3446910/1‑151 (MQ=255)

      cAATCCCTCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGa                                                                                                                                         >  1:373346/1‑151 (MQ=255)

      cAATCCCTCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGa                                                                                                                                         >  2:5320928/1‑151 (MQ=255)

       aaTCCCTCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGAt                                                                                                                                        >  1:2530295/1‑151 (MQ=255)

           ccTCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTc                                                                                                                                    <  2:4369047/151‑1 (MQ=255)

           ccTCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTc                                                                                                                                    >  2:3364076/1‑151 (MQ=255)

             tCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCgg                                                                                                                                  >  1:2463007/1‑151 (MQ=255)

              cGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTCCGGt                                                                                                                                 <  2:5367569/151‑1 (MQ=255)

              cGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCCTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGt                                                                                                                                 <  2:5717401/151‑1 (MQ=255)

                agtaggTTGTCGCGGGCGCCGAACAAGGAGCGGTTGGCGTCGGCTTTGTCGTTGAACGGGTTGGACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCGGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGc                                                                                                                               <  2:2812868/146‑1 (MQ=14)

                aTCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGc                                                                                                                               <  2:4205328/151‑1 (MQ=255)

                aTCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGc                                                                                                                               <  2:2894175/151‑1 (MQ=255)

                  cACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCg                                                                                                                             >  1:3570025/1‑151 (MQ=255)

                    cGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCg                                                                                                                             >  1:5176755/1‑149 (MQ=255)

                       tGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGgtgt                                                                                                                        >  1:1450021/1‑151 (MQ=255)

                        gTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTc                                                                                                                       <  2:778120/151‑1 (MQ=255)

                            cTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCggac                                                                                                                   >  1:2928137/1‑149 (MQ=255)

                            cTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCt                                                                                                                   >  1:4460875/1‑151 (MQ=255)

                            cTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCt                                                                                                                   <  1:1887305/151‑1 (MQ=255)

                            cTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCt                                                                                                                   <  2:2188781/151‑1 (MQ=255)

                             tGGCGCCGAACAAGGAGCCGACGGCGTCGGCTTTGTCCTTGACCGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTGTGAAGCGTGGGTCATGCCCTCCGGTGCCGGGTGTCGGCTg                                                                                                                  >  2:5148443/1‑151 (MQ=14)

                              ggCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTgg                                                                                                                 <  2:275264/151‑1 (MQ=255)

                                 gcCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGccc                                                                                                              <  2:4153879/151‑1 (MQ=255)

                                   cGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCAt                                                                                                            <  1:3964237/151‑1 (MQ=255)

                                   cGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGGAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCAt                                                                                                            >  1:2783465/1‑151 (MQ=255)

                                        aaGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGt                                                                                                       >  1:2864313/1‑151 (MQ=255)

                                         aGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTg                                                                                                      <  1:3634037/151‑1 (MQ=255)

                                         aGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTg                                                                                                      >  1:2540019/1‑151 (MQ=255)

                                         aGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTg                                                                                                      >  1:534878/1‑151 (MQ=255)

                                          ggAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGa                                                                                                     <  1:1952145/151‑1 (MQ=255)

                                            aGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAAt                                                                                                   >  2:3235053/1‑151 (MQ=255)

                                               cGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAg                                                                                                <  2:2606710/151‑1 (MQ=255)

                                               cGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAg                                                                                                <  2:643270/151‑1 (MQ=255)

                                               cGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAg                                                                                                >  1:4609756/1‑151 (MQ=255)

                                                gATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGc                                                                                               >  1:569012/1‑151 (MQ=255)

                                                   ggCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAAc                                                                                            >  2:3561599/1‑151 (MQ=255)

                                                      gTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGACTCAGCAAcccc                                                                                         >  2:4754018/1‑151 (MQ=255)

                                                      gTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGcc                                                                                         >  2:1544593/1‑151 (MQ=17)

                                                      gTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGcc                                                                                         <  1:5964840/151‑1 (MQ=16)

                                                       tCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCa                                                                                        <  1:3857996/151‑1 (MQ=12)

                                                       tCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCa                                                                                        >  1:4330335/1‑151 (MQ=9)

                                                         ggCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGt                                                                                      >  1:6328064/1‑151 (MQ=9)

                                                         ggCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGt                                                                                      <  1:1613280/151‑1 (MQ=17)

                                                         ggCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGt                                                                                      <  1:5320928/151‑1 (MQ=12)

                                                           cTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTga                                                                                    <  1:4119209/151‑1 (MQ=12)

                                                            tttGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTgag                                                                                   >  1:3008887/1‑151 (MQ=9)

                                                               gTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGAtt                                                                                >  2:2103543/1‑151 (MQ=14)

                                                                tCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCACTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCCGTGCCGCGTGTCGGCTGGCCCATTGCGCGAATCAGCAACGCCAGTGCGGttt                                                                               >  2:5967095/1‑151 (MQ=11)

                                                                      aaCGGGTTGAGCGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGAc                                                                         <  2:4561165/151‑1 (MQ=12)

                                                                         gggTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACcgc                                                                      >  1:5738623/1‑151 (MQ=16)

                                                                           gTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAACCAGCAACGCCAGTGCGAGTTCCAGACCccgg                                                                    >  2:442018/1‑151 (MQ=11)

                                                                           gTTGAACGGCACAAAGGGAGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTAGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCgcgg                                                                    <  2:2029078/151‑1 (MQ=11)

                                                                              gAACGGCGCCAAGGCGGTGTAGAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCgcgggcg                                                                 >  2:1447442/1‑151 (MQ=255)

                                                                              gAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCGCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCgcgggcg                                                                 >  2:6310970/1‑151 (MQ=255)

                                                                              gAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACCCCAGTGAGATTTCCAGACCgcgggcg                                                                 >  1:4523878/1‑151 (MQ=255)

                                                                                aCGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGa                                                               <  1:5060481/151‑1 (MQ=255)

                                                                                aCGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTGCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGa                                                               <  2:6154770/151‑1 (MQ=255)

                                                                                   gcgcGAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGt                                                            <  2:5553985/151‑1 (MQ=255)

                                                                                     gcCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGc                                                          >  1:3492562/1‑151 (MQ=21)

                                                                                     gcCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGc                                                          <  2:511541/151‑1 (MQ=21)

                                                                                      ccAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCg                                                         <  2:6244925/151‑1 (MQ=255)

                                                                                           gCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGt                                                    >  2:5924238/1‑151 (MQ=21)

                                                                                           gCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGt                                                    <  1:1751363/151‑1 (MQ=255)

                                                                                             ggTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCa                                                  <  1:4754725/151‑1 (MQ=25)

                                                                                               tgtAACGCGGGCTGTCGTGCTGGTCCAGCCAATGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCAGCTGGCCCATAGGGTGAAACAGCAACGCCAGTGAGATTTCCAGTCCGCGGGCGGAGGTGCGTTCGTCaag                                                <  1:1091475/151‑3 (MQ=11)

                                                                                                gtAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACgg                                               <  1:2142907/151‑1 (MQ=255)

                                                                                                 tAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGt                                              >  1:552813/1‑151 (MQ=255)

                                                                                                  aaaGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTc                                             <  1:2731754/151‑1 (MQ=255)

                                                                                                   aaGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAGGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGTGTTCGTCACGGTCg                                            <  2:1743202/151‑1 (MQ=255)

                                                                                                       gggCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAAc                                        >  2:184188/1‑151 (MQ=255)

                                                                                                        ggCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACa                                       <  2:4909288/151‑1 (MQ=255)

                                                                                                        ggCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACa                                       <  1:653709/151‑1 (MQ=255)

                                                                                                            gTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGcc                                   >  2:5816874/1‑151 (MQ=255)

                                                                                                              cGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTCACAGGCCaa                                 >  2:3712367/1‑151 (MQ=255)

                                                                                                              cGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCaa                                 <  2:3113964/151‑1 (MQ=255)

                                                                                                               gTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAg                                >  2:3383116/1‑151 (MQ=255)

                                                                                                                tGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAgg                               <  1:768305/151‑1 (MQ=255)

                                                                                                                 gCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAggc                              >  1:811019/1‑151 (MQ=255)

                                                                                                                     gTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCaa                                 <  1:1505490/144‑1 (MQ=255)

                                                                                                                        cAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGcc                       <  2:4009577/151‑1 (MQ=255)

                                                                                                                        cAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGcc                       <  1:3141988/151‑1 (MQ=255)

                                                                                                                          gCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAg                     <  1:1740493/151‑1 (MQ=255)

                                                                                                                            cATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGc                   >  1:2383192/1‑151 (MQ=255)

                                                                                                                             aTTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCt                  >  1:127833/1‑151 (MQ=255)

                                                                                                                              ttGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTg                 <  2:2540019/151‑1 (MQ=255)

                                                                                                                              ttGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTg                 <  2:2421900/151‑1 (MQ=255)

                                                                                                                               tGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGt                <  1:999671/151‑1 (MQ=255)

                                                                                                                               tGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGt                <  1:1000558/151‑1 (MQ=255)

                                                                                                                                gCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTa               >  2:860219/1‑151 (MQ=255)

                                                                                                                                gCTCCAGGCGTTTGAAGCGCCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGGCCATTGGGTGAATCAGCAACGCCAGTGCGACTTACAGACCGCGGGCGGAGCTGCGTTCGTCACGGTTGTAAGCGGCCAAGGCGGCTGGCAGGCAGTa               >  2:5790147/1‑151 (MQ=11)

                                                                                                                                  tCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCGGTAgg             >  1:4989180/1‑151 (MQ=255)

                                                                                                                                      ggCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAGGGGtt         <  1:5582966/151‑1 (MQ=255)

                                                                                                                                      ggCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAGGGGtt         >  2:1256077/1‑151 (MQ=255)

                                                                                                                                      ggCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAGGGGtt         >  2:1923847/1‑151 (MQ=255)

                                                                                                                                        cGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAGGGGTTAt       <  1:6169178/151‑1 (MQ=255)

                                                                                                                                         gTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAGGGGTTATc      <  2:3315824/151‑1 (MQ=255)

                                                                                                                                           ttGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCGCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAGGGGTTATCGa    <  1:5936416/151‑1 (MQ=255)

                                                                                                                                             gAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAGGGGTTATCGAtg  <  2:3616582/151‑1 (MQ=255)

CCCCGCCAATCCCTCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAACGCCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAGGGGTTATCGATG  >  CP009974/4540313‑4540604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`