Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 4,541,504 C→T L110L (CTG→CTA)  RPPX_20280 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099744,541,5040CT99.3% 973.7 / NA 287L110L (CTG→CTA) RPPX_20280hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base T (143/141);  total (146/141)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 287 total aligned reads displayed.

GGCGCATGTGCCGTTTGCGCAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGACAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCCGGGCGCAAAGCACGG  >  CP009974/4541356‑4541651
                                                                                                                                                    |                                                                                                                                                   
ggCGCATGTGCCGTTTGCGCAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAg                                                                                                                                                   <  2:2976858/151‑1 (MQ=38)
   gcATGTGCCGTTTGCGCAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTa                                                                                                                                                <  2:1264903/151‑1 (MQ=255)
     aTGTGCCGTTTGCGCAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGCGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAgc                                                                                                                                              >  2:5871044/1‑151 (MQ=255)
     aTGTGCCGTTTGCGCAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCATTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAgc                                                                                                                                              <  2:6285277/151‑1 (MQ=255)
         gCCGTTTGCGCAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCCGGTGTCGGCCCACACCCAGCCGGTGCTCGGGCCGGACAGGCAgcgccc                                                                                                                                          >  2:3398596/1‑149 (MQ=255)
         gCCGTTTGCGCAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTc                                                                                                                                          >  2:2350113/1‑151 (MQ=38)
          ccGTTTGCGCAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCa                                                                                                                                         >  1:3991312/1‑151 (MQ=255)
          ccGTTTGCGCAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCa                                                                                                                                         <  1:2132827/151‑1 (MQ=38)
           cGTTTGCGCAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCAc                                                                                                                                        >  1:6104916/1‑151 (MQ=38)
             tttGCGCAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCAcgg                                                                                                                                      >  2:758630/1‑151 (MQ=38)
                gcgcAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCAcggcgg                                                                                                                                   >  1:5253568/1‑151 (MQ=38)
                 cgcAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCggt                                                                                                                                  <  1:1365764/151‑1 (MQ=38)
                  gcAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCggtg                                                                                                                                 >  2:489544/1‑151 (MQ=38)
                   cAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCggtgg                                                                                                                                >  2:3687229/1‑151 (MQ=38)
                   cAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCggtgg                                                                                                                                >  2:582348/1‑151 (MQ=38)
                    aGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGCGCGGGCTGTAGCCGATCCACACTTCCTTGGCCCTGTCCTGGCAGGGGTCCGCCCACACCCCCCCCGGGCTCTCGCCCCATCGGTCGCGCTCAcggccgctgc                                                                                                                               >  2:3571730/1‑145 (MQ=11)
                     gggCTGCCGAACCGGTGTTACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTa                                                                                                                              <  1:5787631/151‑1 (MQ=255)
                        cTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGtc                                                                                                                           >  1:4192245/1‑151 (MQ=37)
                         tGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGtcc                                                                                                                          >  2:17550/1‑151 (MQ=37)
                             gAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCa                                                                                                                      >  2:512258/1‑151 (MQ=38)
                             gAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCa                                                                                                                      >  2:5176684/1‑151 (MQ=38)
                             gAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCa                                                                                                                      <  1:527047/151‑1 (MQ=255)
                                ccGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCCCTGGCCGGATAGGGAGCGCGCACGGCGGTGGTAGTCCTCCAGcc                                                                                                                   >  2:1078028/1‑151 (MQ=255)
                                 cGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGccc                                                                                                                  >  1:1737176/1‑151 (MQ=38)
                                  ggTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCt                                                                                                                 >  1:1875988/1‑151 (MQ=38)
                                  ggTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTCTCCTGGCAGGTGTCGGCCCACACCCAGCCGCTGCTCTGGCCGGATAGGTAGCGCGCCCGGCGGTGGCAGTCCCCCAccccc                                                                                                                 >  2:5638192/1‑150 (MQ=11)
                                   gTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCtt                                                                                                                >  2:4107569/1‑151 (MQ=38)
                                   gTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCtt                                                                                                                <  1:5879627/151‑1 (MQ=38)
                                   gTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCtt                                                                                                                <  2:5970807/151‑1 (MQ=38)
                                    tGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTg                                                                                                               <  1:1933231/151‑1 (MQ=255)
                                     gATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTgg                                                                                                              >  2:2716973/1‑151 (MQ=38)
                                           ggCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTagctc                                                                                                        <  1:3839337/151‑1 (MQ=255)
                                           ggCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTagctc                                                                                                        <  1:489544/151‑1 (MQ=255)
                                             cGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTagctcag                                                                                                      >  1:2274933/1‑151 (MQ=255)
                                                tGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTagctcagctc                                                                                                   >  1:3654223/1‑151 (MQ=255)
                                                   tGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCttt                                                                                                >  1:1506309/1‑151 (MQ=255)
                                                     gCGTTGGTCCACAGGTGCGGGCGGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGt                                                                                              <  2:3631521/151‑1 (MQ=255)
                                                       gTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTag                                                                                            <  2:2931885/151‑1 (MQ=255)
                                                       gTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTag                                                                                            <  1:5049212/151‑1 (MQ=255)
                                                         tGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTagag                                                                                          <  1:976796/151‑1 (MQ=255)
                                                          ggTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAgg                                                                                         <  2:6415469/151‑1 (MQ=255)
                                                           gTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGc                                                                                        <  2:2568286/151‑1 (MQ=255)
                                                            tCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGTGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGcc                                                                                       >  1:3033694/1‑151 (MQ=255)
                                                            tCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGcc                                                                                       <  2:4216809/151‑1 (MQ=255)
                                                             ccACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGccc                                                                                      <  1:369072/151‑1 (MQ=255)
                                                             ccACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGccc                                                                                      >  1:3056676/1‑151 (MQ=255)
                                                              cacaGGTGCGGGCTGTAGCCGATCCACCCTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCCCGCCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCcg                                                                                     <  1:857804/151‑1 (MQ=255)
                                                               acaGGTGCGGGCTGTAGCCGATCCACACTTCCTGGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCcgc                                                                                    <  1:3352239/151‑1 (MQ=255)
                                                                     gCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGAc                                                                              <  2:4456664/151‑1 (MQ=255)
                                                                      cgcgCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACg                                                                             <  1:2588379/148‑1 (MQ=255)
                                                                       gggCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGt                                                                            >  1:2749005/1‑151 (MQ=255)
                                                                        ggCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACATg                                                                           <  1:2448324/151‑1 (MQ=255)
                                                                           tGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTc                                                                        <  2:1497214/151‑1 (MQ=255)
                                                                           tGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTc                                                                        >  1:4702367/1‑151 (MQ=255)
                                                                            gTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCa                                                                       <  1:790547/151‑1 (MQ=255)
                                                                             tAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCat                                                                      <  1:453931/151‑1 (MQ=255)
                                                                              aGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCatg                                                                     >  1:3542842/1‑151 (MQ=255)
                                                                                ccGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCatgaa                                                                   <  1:1574361/151‑1 (MQ=255)
                                                                                 cGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCatgaat                                                                  <  2:2033325/151‑1 (MQ=255)
                                                                                      cacaCTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACAGCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCa                                                             <  1:555319/151‑1 (MQ=255)
                                                                                       acacTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAg                                                            >  1:2831673/1‑151 (MQ=255)
                                                                                       acacTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGCCGGGATAGGTAGCGCTCACGGCGGGGGGAGTCCTCAAGCCCTTGGGAGCTCAGCTCTTGGTAGAGGCCCGGGCCGACGTGCTCATGAATGCCCAg                                                            >  1:4637828/1‑151 (MQ=14)
                                                                                            tCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCtttt                                                       >  2:466906/1‑151 (MQ=255)
                                                                                             ccTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTc                                                      <  2:140500/151‑1 (MQ=255)
                                                                                               ttGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGc                                                    >  2:3876468/1‑151 (MQ=255)
                                                                                               ttGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGCGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGCGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGc                                                    >  2:189446/1‑151 (MQ=255)
                                                                                                tGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGcc                                                   <  2:3725027/151‑1 (MQ=255)
                                                                                                tGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGcc                                                   >  1:5546112/1‑151 (MQ=255)
                                                                                                   ccGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTg                                                <  2:3267688/151‑1 (MQ=37)
                                                                                                     gtgtCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAg                                              <  2:1162723/151‑1 (MQ=37)
                                                                                                      tgtCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAgg                                             <  1:2311010/151‑1 (MQ=25)
                                                                                                        tCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGc                                           <  2:1587094/151‑1 (MQ=25)
                                                                                                          cTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCt                                         >  2:2131711/1‑151 (MQ=25)
                                                                                                          cTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCt                                         >  2:6088664/1‑151 (MQ=25)
                                                                                                                ggTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATg                                   <  2:6344810/151‑1 (MQ=25)
                                                                                                                 gtgtCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGa                                  <  1:1143929/151‑1 (MQ=25)
                                                                                                                   gtCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAac                                >  2:2807840/1‑151 (MQ=25)
                                                                                                                      ggCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACg                             >  2:948520/1‑151 (MQ=25)
                                                                                                                      ggCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACg                             <  1:3332696/151‑1 (MQ=21)
                                                                                                                       gCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGCCGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCACGACCACGt                            >  2:3977153/1‑151 (MQ=255)
                                                                                                                          cacaCCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGCCCAGCTTTTCGCCCTGAGGGCCTTTCATGAACCCGTCGa                         >  2:6155131/1‑151 (MQ=255)
                                                                                                                          cacaCCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGa                         <  1:4254015/151‑1 (MQ=25)
                                                                                                                           acacCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGAc                        <  1:758630/151‑1 (MQ=25)
                                                                                                                              cccAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATa                     <  1:5435592/151‑1 (MQ=25)
                                                                                                                               ccAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTg                                                >  1:6215629/1‑123 (MQ=37)
                                                                                                                               ccAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAg                    <  1:2466522/151‑1 (MQ=25)
                                                                                                                               ccAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAg                    <  1:6329390/151‑1 (MQ=21)
                                                                                                                               ccAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAg                    >  1:3949431/1‑151 (MQ=25)
                                                                                                                                 aGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGcc                  <  1:1027319/151‑1 (MQ=25)
                                                                                                                                  gCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGcct                 <  1:4377243/151‑2 (MQ=21)
                                                                                                                                  gCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGcct                 >  1:2980117/1‑150 (MQ=18)
                                                                                                                                    cGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTgg               <  1:6152739/151‑1 (MQ=25)
                                                                                                                                    cGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGGGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGTGCCTTTCATGAACACGGAGACATAGCCTgg               >  2:3194758/1‑151 (MQ=255)
                                                                                                                                      gTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGgc             >  1:5581221/1‑151 (MQ=25)
                                                                                                                                        gCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGgcgc           >  2:3314104/1‑151 (MQ=21)
                                                                                                                                           ctGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCaaa        >  1:2531742/1‑151 (MQ=25)
                                                                                                                                             ggCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGc      <  1:2178848/151‑1 (MQ=19)
                                                                                                                                             ggCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGc      >  1:201801/1‑151 (MQ=25)
                                                                                                                                             ggCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTCTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATCGCCTGGGCGCAAAGc      >  2:4289988/1‑151 (MQ=255)
                                                                                                                                              gCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGcg     <  2:4843189/151‑2 (MQ=19)
                                                                                                                                                    |                                                                                                                                                   
GGCGCATGTGCCGTTTGCGCAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGACAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCCGGGCGCAAAGCACGG  >  CP009974/4541356‑4541651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑