BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	4,541,636	C→T	P66P (CCG→CCA)	RPPX_20280 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	4,541,636	0	C	T	99.5%	542.1 / NA	197	P66P (CCG→CCA)	RPPX_20280	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (101/94); total (102/95)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 197 total aligned reads displayed.

CGGTGCTCTGGCCGGACAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCCGGGCGCAAAGCACGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCGGCAGGATAGGCACGCGT  >  CP009974/4541488‑4541783

  gTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGgc                                                                                                                                                 <  2:2749005/151‑1 (MQ=25)

  gTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGgc                                                                                                                                                 >  2:5818162/1‑151 (MQ=25)

  gTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCGGGgc                                                                                                                                                 >  1:6159173/1‑151 (MQ=25)

   tGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGgcg                                                                                                                                                <  1:4660261/151‑1 (MQ=255)

    gCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGgcgc                                                                                                                                               >  2:3314104/1‑151 (MQ=21)

    gCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGgcgc                                                                                                                                               >  1:5905850/1‑151 (MQ=19)

     ctctGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCa                                                                                                                                              >  1:3955466/1‑151 (MQ=21)

      tctGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCaa                                                                                                                                             <  1:1512368/151‑1 (MQ=255)

       ctGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATAAACACGTAGACATAGCCTGGGCGCaaa                                                                                                                                            >  1:5774663/1‑151 (MQ=16)

        tGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAg                                                                                                                                           >  1:2768/1‑151 (MQ=255)

        tGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAg                                                                                                                                           <  2:4709837/151‑1 (MQ=21)

         ggCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGc                                                                                                                                          <  2:2497357/151‑1 (MQ=25)

          gCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGcg                                                                                                                                         <  2:4843189/151‑2 (MQ=19)

           ccGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGcgc                                                                                                                                        >  2:6364117/1‑149 (MQ=255)

            cGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGcgcg                                                                                                                                       <  1:3690106/151‑1 (MQ=255)

              gataGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGa                                                                                                                                     <  1:6146795/148‑1 (MQ=255)

              gataGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGa                                                                                                                                     >  2:2062224/4‑151 (MQ=255)

               ataGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAACGCGCGGAg                                                                                                                                    >  2:4368569/3‑151 (MQ=255)

                  ggTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGgt                                                                                                                                 >  1:4386639/1‑151 (MQ=21)

                    tAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGgtgt                                                                                                                               >  1:5436969/1‑151 (MQ=255)

                      gcgcTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAg                                                                                                                             >  1:6040144/1‑151 (MQ=255)

                      gcgcTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAg                                                                                                                             <  1:186683/151‑1 (MQ=25)

                      gcgcTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAg                                                                                                                             >  1:253762/1‑151 (MQ=255)

                        gcTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGc                                                                                                                           >  1:6329294/1‑151 (MQ=255)

                        gcTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGc                                                                                                                           >  2:5587764/1‑151 (MQ=25)

                         cTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGTGGGTGTAGGcc                                                                                                                          >  1:2009459/1‑151 (MQ=255)

                           cACGGCGGTGGTAGTGCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGa                                                                                                                        <  2:4870657/151‑1 (MQ=255)

                           cACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGa                                                                                                                        >  1:5663255/1‑151 (MQ=255)

                           cACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGa                                                                                                                        >  1:775389/1‑151 (MQ=18)

                            aCGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAg                                                                                                                       <  1:881886/151‑1 (MQ=255)

                              ggcggTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGtg                                                                                                                     >  1:4713742/1‑151 (MQ=255)

                                cggTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGtgct                                                                                                                   <  1:2992956/151‑1 (MQ=25)

                                cggTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGtgct                                                                                                                   >  1:866784/1‑151 (MQ=25)

                                   gggtAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCa                                                                                                                <  2:5507920/150‑1 (MQ=255)

                                    ggtAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAg                                                                                                               >  1:740425/1‑151 (MQ=25)

                                    ggtAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAg                                                                                                               >  1:727685/1‑151 (MQ=255)

                                     gtAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGc                                                                                                              <  2:6104916/151‑1 (MQ=25)

                                     gtAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGc                                                                                                              <  1:2088164/151‑1 (MQ=255)

                                      tAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCg                                                                                                             <  1:3294438/151‑1 (MQ=19)

                                       aGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACCTAGCCTGCGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGt                                                                                                            >  2:3189579/1‑151 (MQ=255)

                                       aGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGt                                                                                                            <  2:5715070/151‑1 (MQ=255)

                                       aGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGt                                                                                                            >  2:2752482/1‑151 (MQ=255)

                                             ccAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGaa                                                                                                      <  1:2350113/151‑1 (MQ=255)

                                             ccAGCCCTTGGTAGCTCAGCGCTTTGTGGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGaa                                                                                                      <  2:6087766/151‑1 (MQ=255)

                                                gCCCTTGGGAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGcc                                                                                                   <  1:2106294/151‑1 (MQ=255)

                                                  ccTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCtt                                                                                                 <  1:3132270/151‑1 (MQ=18)

                                                  ccTTGGGAGCTCAGCTCTTTGTACAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATCGTCTGGGCGCAAAGCGAGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCtt                                                                                                 <  2:1371129/151‑1 (MQ=11)

                                                   cTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTg                                                                                                <  1:3464455/151‑1 (MQ=16)

                                                   cTTGGTAGCTCAGCTCTGTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTg                                                                                                <  2:4504542/151‑1 (MQ=255)

                                                    ttGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGt                                                                                               >  1:869022/1‑151 (MQ=19)

                                                     tGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGtt                                                                                              >  1:5060688/1‑151 (MQ=19)

                                                      ggTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTc                                                                                             >  2:308960/1‑151 (MQ=16)

                                                        tAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCa                                                                                           <  1:4816585/151‑1 (MQ=9)

                                                        tAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGGGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCa                                                                                           >  1:5579398/1‑151 (MQ=9)

                                                         agctcagctcTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAg                                                                                          >  1:1260393/1‑151 (MQ=9)

                                                         agctcagctcTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAg                                                                                          <  2:1737176/151‑1 (MQ=9)

                                                          gctccgctcTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAgg                                                                                         <  2:2991763/151‑1 (MQ=9)

                                                                                                gagtgaCCAGCTTTTCGCCCTGGGGGCCTTTCATGGACGCGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTc                                                   <  2:5656372/148‑1 (MQ=1)

                                                                                                       cAGCTTTTCGCCCTGAGGGCCTTTCATGCACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCCGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGGCGCCGGTg                                            >  2:4111997/1‑151 (MQ=11)

                                                                                                       cAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTg                                            >  2:768600/1‑151 (MQ=9)

                                                                                                        aGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGc                                           >  2:4281630/1‑151 (MQ=9)

                                                                                                         gCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCg                                          <  2:3071058/151‑1 (MQ=14)

                                                                                                          cgtttCGCGCTGGGGGCCGTTCCTGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGGGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCgg                                         <  1:3849453/149‑1 (MQ=11)

                                                                                                          cTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCgg                                         >  2:683294/1‑151 (MQ=14)

                                                                                                            tttCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCgggg                                       >  2:2153441/1‑151 (MQ=9)

                                                                                                             ttCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGc                                      <  2:1904889/151‑1 (MQ=17)

                                                                                                                gCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACg                                   >  1:2310005/1‑151 (MQ=9)

                                                                                                                 cccTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGa                                  <  2:3787884/151‑1 (MQ=17)

                                                                                                                   cTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATg                                >  1:3378223/1‑151 (MQ=9)

                                                                                                                   cTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATg                                >  1:3566452/1‑151 (MQ=12)

                                                                                                                    tGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATgg                               >  2:2205755/1‑151 (MQ=9)

                                                                                                                     gAGGGCCGTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGc                              <  2:6304087/151‑1 (MQ=9)

                                                                                                                      aGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCg                             >  2:4187563/1‑151 (MQ=9)

                                                                                                                      aGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCg                             >  1:950031/1‑151 (MQ=14)

                                                                                                                        ggCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTa                           <  1:4557611/151‑1 (MQ=12)

                                                                                                                          ccTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAgc                         >  1:1352544/1‑151 (MQ=9)

                                                                                                                          ccTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAgc                         <  2:3654223/151‑1 (MQ=17)

                                                                                                                           cTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAgcg                        >  2:2891522/1‑151 (MQ=14)

                                                                                                                             ttCATGAACACGTAGACATGGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCa                      <  2:2613319/151‑1 (MQ=12)

                                                                                                                                gtGAACACGGAGACCTGGGCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGCGTGCTGCAGCGTGGGGAGGCCTTGTTACTGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCg                   <  2:2290640/150‑1 (MQ=11)

                                                                                                                                aTGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCg                   >  2:4815194/1‑151 (MQ=16)

                                                                                                                                 tGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCgg                  >  1:5637874/1‑151 (MQ=16)

                                                                                                                                  gAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCGGc                 >  1:4238732/1‑151 (MQ=18)

                                                                                                                                  gAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCGGc                 >  2:3043698/1‑151 (MQ=16)

                                                                                                                                    acacGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCGGCAg               >  1:446184/1‑151 (MQ=255)

                                                                                                                                      acGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCGGCAGGa             >  1:1589899/1‑151 (MQ=19)

                                                                                                                                       cGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCGGCAGGAt            >  2:3249095/1‑151 (MQ=16)

                                                                                                                                       cGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCGGCAGGAt            <  1:189284/151‑1 (MQ=25)

                                                                                                                                         tAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCGGCAGGATAg          <  1:1108648/151‑1 (MQ=255)

                                                                                                                                          aGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCGGCAGGATAgg         <  1:797533/151‑1 (MQ=255)

                                                                                                                                           gACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCGGCAGGAGAGGc        >  2:1276891/1‑151 (MQ=255)

                                                                                                                                            aCATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCGGCAGGATAGGCa       >  1:1130671/1‑151 (MQ=255)

                                                                                                                                             cATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCGGCAGGATAGGCAc      >  1:1035262/1‑151 (MQ=255)

                                                                                                                                             cATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCGGCAGGATAGGCAc      <  1:5216161/151‑1 (MQ=255)

                                                                                                                                             cATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCGGCAGGATAGGCAc      <  1:4504985/151‑1 (MQ=255)

                                                                                                                                               tAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCGGCAGGATAGGCAcgc    >  1:2505192/1‑151 (MQ=255)

                                                                                                                                               tAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCGGCAGGATAGGCAcgc    <  2:5109279/151‑1 (MQ=255)

                                                                                                                                               tAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCGGCAGGATAGGCAcgc    >  1:1052336/1‑151 (MQ=255)

                                                                                                                                                aGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCGGCAGGATAGGCAcgcg   <  1:6249794/151‑1 (MQ=255)

                                                                                                                                                 gCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCGGCAGGATAGGCACGCGt  <  2:3199592/151‑1 (MQ=255)

CGGTGCTCTGGCCGGACAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCCGGGCGCAAAGCACGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCGGCAGGATAGGCACGCGT  >  CP009974/4541488‑4541783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`