Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	4,543,833	C→G	S430S (TCG→TCC)	RPPX_20290 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	4,543,833	0	C	G	100.0%	53.9 / NA	39	S430S (TCG→TCC)	RPPX_20290	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (24/15);  total (24/15)

CTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAAGCCGTCTTGAGCGATAGGATGTTGAACCACAGCATCCCCGCGTTTAAACCACCACCACCGCCCGAGGTGAGCAACCCAAGGAAGTTGGGTTTCACACGCGCAGTGGTGGTCAGCTCGATAGACACCGTGCGGCTACCCGAGATGCGCAACGCTTTCATCTCTTCAACCTGTGCCATGCCGCTCTTCAAATACCGCTGCACCTCCTGGCTAA  >  CP009974/4543685‑4543981                                                                                                                                                     |                                                                                                                                                     ctgccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGa                                                                                                                                                    >  1:4138017/1‑151 (MQ=11) ctgccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGa                                                                                                                                                    >  1:636036/1‑151 (MQ=11)  tgccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAg                                                                                                                                                   <  2:826590/151‑1 (MQ=11)  tgccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAg                                                                                                                                                   <  2:6186600/151‑1 (MQ=11)  tgccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAg                                                                                                                                                   >  2:5174599/1‑151 (MQ=11)   gccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAgg                                                                                                                                                  >  1:4279747/1‑151 (MQ=11)    ccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGt                                                                                                                                                 >  1:5470981/1‑151 (MQ=11)     cgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTg                                                                                                                                                >  2:1188581/1‑151 (MQ=11)      gctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGa                                                                                                                                               >  2:1828586/1‑151 (MQ=11)      gctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGa                                                                                                                                               <  1:5888457/151‑1 (MQ=11)      gctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGa                                                                                                                                               <  1:863222/151‑1 (MQ=11)       ctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAg                                                                                                                                              >  1:4407117/1‑151 (MQ=11)       ctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTCAACCACAGCATCCCTGCGCTTAAACCCCCACCACCGCCGGAggcgcg                                                                                                                                              >  2:516149/1‑147 (MQ=11)         gcgcCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCATGCAGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGAAGGTGAGCa                                                                                                                                            >  2:3943642/1‑151 (MQ=11)         gcgcCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGcc                                                                                                                                            >  2:348083/1‑150 (MQ=11)         gcgcCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCa                                                                                                                                            >  2:1664907/1‑151 (MQ=11)          cgcCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCaa                                                                                                                                           <  1:1962322/151‑1 (MQ=11)           gcCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCACTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAAc                                                                                                                                          >  1:3993272/1‑151 (MQ=11)            ccAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAAcc                                                                                                                                         >  2:1500033/1‑151 (MQ=11)              aaTCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGCGGTGAGCAACCCa                                                                                                                                       >  2:3081989/1‑151 (MQ=11)              aaTCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCa                                                                                                                                       <  2:3921481/151‑1 (MQ=1)               aTCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCaa                                                                                                                                      >  1:6122565/1‑151 (MQ=11)                 cACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAgg                                                                                                                                    >  2:4955369/1‑151 (MQ=11)                 cACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAgg                                                                                                                                    <  1:5405317/151‑1 (MQ=11)                  aCCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGa                                                                                                                                   <  1:4932378/151‑1 (MQ=11)                   ccccGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGaa                                                                                                                                  <  1:896394/151‑1 (MQ=11)                    cccGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAg                                                                                                                                 <  2:4168596/151‑1 (MQ=11)                      cGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAGtt                                                                                                                               <  2:5187206/151‑1 (MQ=11)                      cGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAGtt                                                                                                                               <  1:5282819/151‑1 (MQ=11)                       gAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAGTTg                                                                                                                              >  2:1120153/1‑151 (MQ=11)                       gAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAGTTg                                                                                                                              <  2:5629991/151‑1 (MQ=11)                        aaCACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCACCCCCAGGAAGTTgg                                                                                                                             >  2:4424779/1‑151 (MQ=11)                         acacAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAGTTggg                                                                                                                            >  2:2953783/1‑151 (MQ=11)                         acacAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAGTTggg                                                                                                                            >  2:1942157/1‑151 (MQ=11)                         acacAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAGTTggg                                                                                                                            >  2:1747239/1‑151 (MQ=11)                          cacaGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAGTTGGGt                                                                                                                           >  1:4630785/1‑151 (MQ=1)                          cacaGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAGTTGGGt                                                                                                                           <  1:5851121/151‑1 (MQ=1)                          cacaGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAGTTGGGt                                                                                                                           >  1:884812/1‑151 (MQ=11)                          cacaGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAGTTGGGt                                                                                                                           <  1:1127720/151‑1 (MQ=1)                                                                                                                                                   cccGAGGTGAGCAACCCAAGGAAGTTGGGTTTCACACGCGCAGTGGTGGTCAGCTCGATAGACACCGTGCGGCTACCCGAGATGCGCAACGCTTTCATCTCTTCAACCTGCGCCATGCCGCTCTTCAAATACCGCTGCACCTCCTGCCTaa  >  2:802389/1‑151 (MQ=14)                                                                                                                                                     |                                                                                                                                                     CTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAAGCCGTCTTGAGCGATAGGATGTTGAACCACAGCATCCCCGCGTTTAAACCACCACCACCGCCCGAGGTGAGCAACCCAAGGAAGTTGGGTTTCACACGCGCAGTGGTGGTCAGCTCGATAGACACCGTGCGGCTACCCGAGATGCGCAACGCTTTCATCTCTTCAACCTGTGCCATGCCGCTCTTCAAATACCGCTGCACCTCCTGGCTAA  >  CP009974/4543685‑4543981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑