BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	4,544,682	T→G	S147S (TCA→TCC)	RPPX_20290 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	4,544,682	0	T	G	98.2%	525.8 / ‑1.3	171	S147S (TCA→TCC)	RPPX_20290	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/3); new base G (85/83); total (85/86)
Fisher's exact test for biased strand distribution p-value = 2.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 171 total aligned reads displayed.

AGGCCAGGTTGCGCAGGGCGGCAATCCGCCGTTCCAGCGCGTCGCGTTCGGCGAGGAAGCGCCGGTATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGTGACTCGGCCGGCGACAGGGCGGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGG  >  CP009974/4544540‑4544829

                                 ccggcgcGTCGCGTTCGGCGAGGAAGCGCCGGTATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGTGACTCGGCCGGCGACAGGGCGGGCCCCCTGCGTGAAGTGcc                                                                                                            <  2:5901077/148‑1 (MQ=1)

                                                       gaggcgcGGGTATTTGTCGAGATCGAGGCGCTGTGCGAGGCGCGCGCCATTGGGGGAGTGATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTcc                                                                                      <  2:5981875/148‑1 (MQ=1)

                                                             ccGGTATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAg                                                                                <  2:2401940/151‑1 (MQ=2)

                                                              gggTATTTGTCGACGTCGAGGCGCTGGGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAgg                                                                               <  2:6083696/150‑1 (MQ=255)

                                                               ggTATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGCGAggg                                                                              >  1:326451/1‑151 (MQ=9)

                                                                gTATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAgggg                                                                             >  1:3597986/1‑151 (MQ=12)

                                                                gTATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAgggg                                                                             <  1:3662893/151‑1 (MQ=12)

                                                                  atgtGTCGCCATCGAGGCGCTGTGCGAGGCGCGCGCCGTTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGCCTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGc                                                                           <  2:5630396/148‑1 (MQ=11)

                                                                  aTTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGAGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGc                                                                           >  1:1791894/1‑151 (MQ=255)

                                                                   tttGTCGACAGCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCa                                                                          <  2:2835296/151‑1 (MQ=16)

                                                                     tGTCGACATCGAGGCGCTGTGGCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGGGAGGTGCCCAGCCTGAACGGGGGCGGTGCCGGTGAGGGGGCAtg                                                                        >  2:731507/1‑150 (MQ=11)

                                                                     tGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATc                                                                        <  1:5217306/151‑1 (MQ=255)

                                                                     tGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATc                                                                        <  1:4737401/151‑1 (MQ=255)

                                                                     tGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATc                                                                        >  2:735608/1‑151 (MQ=21)

                                                                      gtggACATCGAGGCGCGGGGCCGGGCGTGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATcc                                                                       <  2:4858629/148‑1 (MQ=11)

                                                                      gTCGACATCGAGGCGCTGTGCCAGGCGGGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATcc                                                                       <  2:4951258/151‑1 (MQ=255)

                                                                      gTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCCTcc                                                                       >  2:5029212/1‑151 (MQ=255)

                                                                      gTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATcc                                                                       >  1:4163716/1‑151 (MQ=255)

                                                                      gTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATcc                                                                       <  1:64922/151‑1 (MQ=255)

                                                                      gTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATcc                                                                       <  1:1117866/151‑1 (MQ=255)

                                                                        cGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATccgc                                                                     >  1:1148299/1‑151 (MQ=255)

                                                                        cGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATccgc                                                                     >  1:710688/1‑151 (MQ=255)

                                                                          aCATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATccgccg                                                                   >  2:4214691/1‑151 (MQ=16)

                                                                          aCATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATccgccg                                                                   >  2:2945132/1‑151 (MQ=18)

                                                                          aCATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATccgccg                                                                   <  2:467774/151‑1 (MQ=255)

                                                                           cATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCgg                                                                  >  2:888917/1‑151 (MQ=18)

                                                                           cATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCgg                                                                  <  1:3862705/151‑1 (MQ=255)

                                                                            aTCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGGTCCGGTGAGGGGGCATCCGCCggg                                                                 >  2:1648747/1‑151 (MQ=255)

                                                                             gcGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGt                                                                <  2:4317570/150‑1 (MQ=255)

                                                                              cGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTa                                                               >  1:3295404/1‑151 (MQ=255)

                                                                               gAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTAc                                                              >  1:4197760/1‑151 (MQ=255)

                                                                               gAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTAc                                                              >  2:240512/1‑151 (MQ=19)

                                                                               gAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTAc                                                              >  1:5814311/1‑151 (MQ=255)

                                                                               gAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTAc                                                              >  2:2400982/1‑151 (MQ=19)

                                                                                aGGCGCTGTGCCAGGCGCGCGCCATTGGGGGCGTAATCTTCATGGGCGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTCCCGGCGCGGGGGCATCCGCCGGGTAcc                                                             >  2:5138000/1‑150 (MQ=11)

                                                                                aGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACa                                                             <  2:5576166/151‑1 (MQ=25)

                                                                                   cgcTGTGCCAGGCGCGCGCCCTTGGGGCAGCAGTCTTCAGGGGCGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGGGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGGCGGGTACAGGc                                                          >  2:2232571/1‑151 (MQ=11)

                                                                                    gcTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGct                                                         >  1:4360758/1‑151 (MQ=255)

                                                                                     cTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGctc                                                        <  1:4258949/151‑1 (MQ=255)

                                                                                     cTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGctc                                                        <  1:479566/151‑1 (MQ=255)

                                                                                       gtgCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCtctc                                                      <  2:215803/151‑1 (MQ=255)

                                                                                       gtgCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCtctc                                                      <  2:6123597/151‑1 (MQ=255)

                                                                                         gCCAGGCGCGCGCCATTGGGGTAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCa                                                    >  2:414222/1‑151 (MQ=255)

                                                                                          ccAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAg                                                   <  2:6166873/151‑1 (MQ=255)

                                                                                          ccAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCCGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGGACAGGCTCTCCac                                                   >  2:1432247/1‑150 (MQ=255)

                                                                                            aGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCGGCg                                                 >  2:120288/1‑151 (MQ=255)

                                                                                            aGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCg                                                 >  1:1087098/1‑151 (MQ=255)

                                                                                              gcgcgcgtCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTc                                               <  2:4022080/151‑1 (MQ=255)

                                                                                                gcgcgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCtt                                             <  1:6053085/151‑1 (MQ=255)

                                                                                                 cgcggCATTGGGGGAGTAATCTTAATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTg                                            <  2:1683192/151‑1 (MQ=255)

                                                                                                   cgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGtt                                          >  1:5431835/1‑151 (MQ=255)

                                                                                                   cgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGtt                                          <  1:2492881/151‑1 (MQ=255)

                                                                                                   cgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGtt                                          <  1:3303856/151‑1 (MQ=255)

                                                                                                    gcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTc                                         >  1:531831/1‑151 (MQ=255)

                                                                                                    gcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTc                                         >  2:1188757/1‑151 (MQ=255)

                                                                                                    gcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTc                                         >  1:244750/1‑151 (MQ=255)

                                                                                                    gcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTc                                         <  1:3697050/151‑1 (MQ=255)

                                                                                                     ccATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGCAGTGCCCAGCCTGAACGCGGCCAGGTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGCCCTGTTca                                        >  2:3508572/1‑151 (MQ=11)

                                                                                                     ccATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTca                                        <  2:1267970/151‑1 (MQ=255)

                                                                                                      cATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTcag                                       >  1:4623788/1‑151 (MQ=255)

                                                                                                        ttGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTcagca                                     >  1:1869851/1‑151 (MQ=255)

                                                                                                         tGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTcagcag                                    >  2:3835206/1‑151 (MQ=255)

                                                                                                         tGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTcagcag                                    >  2:3748789/1‑151 (MQ=255)

                                                                                                         tGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCACCCTGCGCGGAGTGCCCGGCCTGGACGCGGCCAGGTCCGGGGAGGTGGCATCCGCCGGGTACAGCCTCTCCAGCGCCTTGTTcaacag                                    >  2:5281569/1‑151 (MQ=11)

                                                                                                          gggggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCagcagc                                   >  1:2849852/1‑151 (MQ=255)

                                                                                                          gggggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCagcagc                                   <  1:2638575/151‑1 (MQ=255)

                                                                                                           ggggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAgcagca                                  <  2:52683/151‑1 (MQ=255)

                                                                                                             ggAGTAATCTTCATGGGTGAGGGGCTGGAAATGGGGCTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATg                                <  2:4275774/151‑1 (MQ=255)

                                                                                                             ggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGACCCCTGCGTGAAGTGCCCAGCCTGCACGCGGTCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTACAGCAGCATg                                >  2:1825009/1‑151 (MQ=11)

                                                                                                               aGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGc                              <  1:6011923/151‑1 (MQ=255)

                                                                                                                gTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCg                             <  2:96377/151‑1 (MQ=255)

                                                                                                                gTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCg                             >  2:4809271/1‑151 (MQ=255)

                                                                                                                gTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCg                             <  1:4043022/151‑1 (MQ=255)

                                                                                                                gTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCCGCCAGTCCCGGTGAGGGGGCATCCGCCGGGTACAGGCGCTCCAGCGTCGTGTTCCGCAGCATGGCg                             >  2:268078/1‑151 (MQ=11)

                                                                                                                  aaTCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGAc                           <  1:3337295/151‑1 (MQ=255)

                                                                                                                   aTCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGAcc                          <  1:1454566/151‑1 (MQ=255)

                                                                                                                   aTCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGAcc                          >  1:71811/1‑151 (MQ=255)

                                                                                                                    tCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCa                         >  1:1018616/1‑151 (MQ=255)

                                                                                                                    tCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCa                         >  1:1018462/1‑151 (MQ=255)

                                                                                                                    tCTTCATGGGTGAGGGCCGGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACGGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCa                         <  2:2553815/151‑1 (MQ=255)

                                                                                                                     cTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAg                        <  1:2822740/151‑1 (MQ=255)

                                                                                                                     cTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAg                        <  2:1087098/151‑1 (MQ=255)

                                                                                                                         aggggTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTg                    <  1:6310081/149‑1 (MQ=255)

                                                                                                                           gggTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCg                  >  1:4965296/1‑151 (MQ=255)

                                                                                                                            ggTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGa                 <  1:57160/151‑1 (MQ=255)

                                                                                                                            ggTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGCTTGCGa                 >  2:2407724/1‑151 (MQ=255)

                                                                                                                             gTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTGGTTCAGCAGCATGGCGACCAGGTTGCGAt                <  2:3844742/151‑1 (MQ=255)

                                                                                                                               gAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATgg              >  1:23700/1‑151 (MQ=255)

                                                                                                                               gAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCAGCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATgg              <  2:3039109/151‑1 (MQ=255)

                                                                                                                               gAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCAGGTGAGGGGGCATCCGCCGGGTACAGGCTCCCCCGCGTCCTGTTCAGCAGCATGGCGACCAGGTTGCGATgg              >  1:1491305/1‑151 (MQ=11)

                                                                                                                                aGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGt             >  2:4135589/1‑151 (MQ=255)

                                                                                                                                  ggCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGa           >  1:1834564/1‑151 (MQ=255)

                                                                                                                                     cTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAg        >  1:2140460/1‑151 (MQ=255)

                                                                                                                                      tGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCCCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGt       <  1:5553303/151‑1 (MQ=255)

                                                                                                                                       ggAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTc      <  1:5060423/151‑1 (MQ=255)

                                                                                                                                       ggAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTc      >  2:5777897/1‑151 (MQ=255)

                                                                                                                                       ggAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTc      >  1:4360975/1‑151 (MQ=255)

                                                                                                                                       ggAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTc      >  1:3071808/1‑151 (MQ=255)

                                                                                                                                         aaaTGGGACTCGGCCGGCGACAGGGCAGGCTCCCGGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCAGGGTACAGGCGCGCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTcgc    <  2:3413416/151‑1 (MQ=11)

                                                                                                                                           aTGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCgg  >  1:1385216/1‑151 (MQ=255)

AGGCCAGGTTGCGCAGGGCGGCAATCCGCCGTTCCAGCGCGTCGCGTTCGGCGAGGAAGCGCCGGTATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGTGACTCGGCCGGCGACAGGGCGGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGG  >  CP009974/4544540‑4544829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`