Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	4,631,683	G→A	intergenic (+245/‑585)	emrB → / → RPPX_20645	multidrug resistance protein B/oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	4,631,683	0	G	A	100.0%	158.9 / NA	52	intergenic (+245/‑585)	emrB/RPPX_20645	multidrug resistance protein B/oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/32);  total (20/32)

AGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTG  >  CP009974/4631536‑4631712                                                                                                                                                    |                              gggcggggCGTGGGGCTGGCGAAGGGGGGGGACGGGCATGGATGCCCGGCAAGCGCTGAGGCCCGATGGGGGGGGCCGGCGGCGCGTACTCCGGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGa                            <  2:2818399/144‑1 (MQ=11) gggCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGa                            <  2:4620135/150‑1 (MQ=255) aggCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGa                            >  1:5481715/1‑151 (MQ=255) aggCCGAGCGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACTGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGa                            >  2:5707798/1‑151 (MQ=255) aggCCGAGCGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGa                            <  2:3645183/151‑1 (MQ=25)  ggCCGAGCGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAg                           <  1:4236404/151‑1 (MQ=25)  ggCCGAGCGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAg                           >  1:3057055/1‑151 (MQ=255)    ccGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAgcc                         >  2:3294633/1‑150 (MQ=255)    ccGAGCGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAgcc                         >  2:2257593/1‑150 (MQ=255)    ccGAGCGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAgcc                         >  1:3188630/1‑150 (MQ=255)     cGAGCGTGGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAgcca                        <  1:1532800/151‑3 (MQ=255)     cGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAgcca                        >  2:3106460/1‑149 (MQ=255)     cGAGCGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAgcca                        >  1:781222/1‑149 (MQ=255)     cGAGCGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAgcca                        >  1:883798/1‑149 (MQ=255)      gAGCGGAGGGCTTGCGAAGGGAGGTGACGGGGATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAgccag                       <  1:6312362/151‑4 (MQ=255)      gAGCGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAgccag                       <  1:6387779/151‑4 (MQ=255)        gcgcAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCg                    <  2:787724/151‑1 (MQ=255)        gcgcAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCg                    >  1:1340265/1‑151 (MQ=255)         cgcaGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGt                   >  2:4067199/4‑151 (MQ=255)         cGTAGGGCTGGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGt                   <  2:541925/151‑1 (MQ=255)          gTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGCGCGTAGCGCAGGGGCCGGATGATAGGAgcgagaggg                   >  1:238978/1‑145 (MQ=255)           caGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGttt                 >  1:3635831/2‑151 (MQ=255)            agagCTGGCGAAGGGAGGTGACGGGCATGGATGCACGAAAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCACTGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttt                <  1:1749790/148‑1 (MQ=11)            aGGGCTTGCGCAGGGACGGGACGGGCCTGGATGCCCATCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttt                <  2:4063780/151‑1 (MQ=11)            aGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttt                <  1:673961/151‑1 (MQ=255)            aGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttt                <  1:4637306/151‑1 (MQ=255)            aGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttt                >  1:4533901/1‑151 (MQ=255)            aGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttt                <  1:2527749/151‑1 (MQ=255)             gggCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGttttt               <  1:5368072/151‑1 (MQ=255)             gggCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGttttt               <  1:5259179/151‑1 (MQ=255)              ggCTTGGGAAGGGAGGTGCCGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttttt              <  2:4279162/151‑1 (MQ=255)              ggCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGTCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttttt              <  1:2106339/151‑1 (MQ=255)              ggCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttttt              <  1:1026962/151‑1 (MQ=255)               gCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTg             <  1:905430/151‑1 (MQ=255)                cTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTgg            >  2:4800035/1‑151 (MQ=255)                 ttGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGt           <  2:2034849/151‑1 (MQ=16)                 ttGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGt           >  2:3473759/1‑151 (MQ=255)                 ttGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGCGTTTTGGt           >  2:1382850/1‑151 (MQ=255)                 ggacGAAGGGAGGTGGCGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGGATGGGGCCTGCAGAGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGt           <  2:3201622/148‑1 (MQ=11)                   gCGAAGGGAGGTGCCGGGCACGGATGCCCGCCAAGCGCTGAGGCCCCATGGATGGGGCCTGCCGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTa         <  2:917834/151‑1 (MQ=11)                    ggAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTAc        <  1:4473060/150‑1 (MQ=16)                    cGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTAc        >  1:3075767/1‑151 (MQ=255)                    cGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTAc        <  1:5947040/151‑1 (MQ=255)                     gAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACt       <  2:1650069/151‑1 (MQ=16)                      aaGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACtt      <  1:2653372/151‑1 (MQ=12)                      aaGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACtt      <  1:1696562/151‑1 (MQ=12)                         ggAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACttttt   >  1:2825174/1‑151 (MQ=9)                         ggAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACttttt   <  2:4476460/151‑1 (MQ=9)                          gAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACTTTTTg  <  2:1473986/151‑1 (MQ=2)                          gAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACTTTTTg  >  2:6166796/1‑151 (MQ=2)                          gAGGTGACGGGCAGGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACTTTTTg  <  1:2370589/151‑1 (MQ=2)                          gAGGTAACGGGCGTGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACTTTTTg  <  1:2088589/151‑1 (MQ=255)                                                                                                                                                    |                              AGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTG  >  CP009974/4631536‑4631712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑