BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	4,957,191	G→A	A208A (GCG→GCA)	RPPX_21945 →	ATPase AAA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	4,957,191	0	G	A	99.6%	550.3 / NA	242	A208A (GCG→GCA)	RPPX_21945	ATPase AAA
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (106/135); minor base T (1/0); total (107/135)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 242 total aligned reads displayed.

GACTGACCTGCTGATCTTGGACGACTGGGGCCTGGCACCGTTTACCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTCACCAGCCAGATGCCGGTGGACAAATGGCATGCGCTGATCGGCGATCCGACCTTGGGCGACGCGATCCTCGACCGTCTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGACTTCAGACTAACAATGCAACCCTGC  >  CP009974/4957047‑4957337

gACTGACCTGCTGATCTTGGACGACTGGGGCCTGGCACCGTTTACCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATc                                                                                                                                              <  1:366141/151‑1 (MQ=255)

gACTGACCTGCTGATCTTGGACGACTGGGGCCTGGCACCGTTTACCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGAGGATCAATGGCATGCACTGATc                                                                                                                                              >  2:4676598/1‑151 (MQ=255)

    gACCTGCTGATCTTGGACGACTGGGGCCTGGCACCGTTTACCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCg                                                                                                                                          <  2:1885366/151‑1 (MQ=255)

     aCCTGCTGATCTTGGACGACTGGGGCCTGGCACCGTTTACCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGa                                                                                                                                         <  1:3099024/151‑1 (MQ=255)

      ccTGCTGATCTTGGACGACTGGGGCCTGGCACCGTTTACCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGAt                                                                                                                                        <  1:2584425/151‑1 (MQ=255)

       ctgctgATCTTGGACGACTGGGGCCTGGCACCGTTTACCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATc                                                                                                                                       <  2:3360402/151‑1 (MQ=255)

          ctgATCTTGGACGACTGGGGCCTGGCACCGTTTACCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGa                                                                                                                                    >  1:6009542/1‑151 (MQ=255)

          ctgATCTTGGACGACTGGGGCCTGGCACCGTTTACCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGa                                                                                                                                    <  2:2852881/151‑1 (MQ=255)

           tgATCTTGGACGACTGGGGCCTGGCACCGTTTACCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGAc                                                                                                                                   <  2:2292160/151‑1 (MQ=255)

            gATCTTGGACGACTGGGGCCTGGCACCGTTTACCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGAcc                                                                                                                                  <  1:85692/151‑1 (MQ=255)

             aTCTTGGACGACTGGGGCCTGGCACCGTTTACCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCt                                                                                                                                 <  1:5533458/151‑1 (MQ=255)

              tCTTGGACGACTGGGGCCTGGCACCGTTTACCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCtt                                                                                                                                >  1:1102969/1‑151 (MQ=255)

                    acgacTGGGGCCTGGCACCGTTTACCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGa                                                                                                                          <  1:5462805/151‑1 (MQ=255)

                    acgacTGGGGCCTGGCACCGTTTACCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGa                                                                                                                          <  1:3909860/151‑1 (MQ=255)

                     cgacTGGGGCCTGGCACCGTTTACCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGat                                                                                                                         <  1:5218310/151‑2 (MQ=255)

                       ccTGGGGCCTGGCACCGTTTACCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGAtgc                                                                                                                       <  2:5969762/150‑1 (MQ=11)

                       acTGGGGCCTGGCACCGTTTACCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGatgc                                                                                                                       >  1:4426118/1‑148 (MQ=255)

                         tGGGGCCTGGCACCGTTTACCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGa                                                                                                                     >  1:1589644/1‑151 (MQ=14)

                           gggCCTGGCACCGTTTACCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCACCCGCTCGACAATGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATc                                                                                                                   <  1:4036318/151‑1 (MQ=11)

                             gCCTGGCACCGTTTACCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCt                                                                                                                 <  2:3144340/151‑1 (MQ=11)

                                tGGCACCGTTTACCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGa                                                                                                              >  1:5611685/1‑151 (MQ=11)

                                 ggCACCGTTTACCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGACCGGCGATCCGACCTTGGGCGATGCGATCCTCGAc                                                                                                             >  2:5707628/1‑151 (MQ=11)

                                   cACCGTTTACCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCg                                                                                                           >  2:1669826/1‑151 (MQ=255)

                                      cGTTTACCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCggtt                                                                                                        <  1:2515956/151‑4 (MQ=11)

                                       gTTTACCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGAGATCATCGACCggttg                                                                                                       >  1:976387/1‑147 (MQ=11)

                                        tttACCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCggttgg                                                                                                      <  2:922946/151‑6 (MQ=11)

                                           aCCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGc                                                                                                   >  1:6128989/1‑151 (MQ=11)

                                              gcagcaCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCTCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGcaca                                                                                                <  1:6255764/151‑1 (MQ=11)

                                                aGCGGACCGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAAc                                                                                              <  2:197949/151‑1 (MQ=11)

                                                aGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAAc                                                                                              <  2:295623/151‑1 (MQ=11)

                                                 gcgcAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACg                                                                                             <  1:2486682/151‑1 (MQ=11)

                                                   gcAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGCTGCCGGGGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCt                                                                                           >  2:3332465/1‑151 (MQ=11)

                                                   gcAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCt                                                                                           >  1:6534482/1‑151 (MQ=11)

                                                   gcAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCt                                                                                           >  2:5576757/1‑151 (MQ=11)

                                                    cAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCtt                                                                                          >  1:17051/1‑151 (MQ=11)

                                                    cAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCtt                                                                                          <  2:4262748/151‑1 (MQ=11)

                                                    cAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCtt                                                                                          <  1:3364085/151‑1 (MQ=11)

                                                     aaCGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTa                                                                                         >  1:267309/1‑151 (MQ=11)

                                                     aaCGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTa                                                                                         <  1:5631867/151‑1 (MQ=11)

                                                     aaCGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTa                                                                                         <  2:309742/151‑1 (MQ=11)

                                                      aCGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTAt                                                                                        >  2:3569098/1‑151 (MQ=11)

                                                      aCGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTAt                                                                                        <  1:4289349/151‑1 (MQ=11)

                                                                                      gCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCaa                                                        <  1:4324731/151‑1 (MQ=11)

                                                                                      gCTACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCaa                                                        >  2:4584031/1‑151 (MQ=11)

                                                                                        tACGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGc                                                      <  2:6330237/151‑1 (MQ=11)

                                                                                         aCGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCg                                                     <  2:2742840/151‑1 (MQ=11)

                                                                                         aCGGCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCg                                                     <  2:1242374/151‑1 (MQ=11)

                                                                                            gCAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGc                                                  <  1:802851/151‑1 (MQ=11)

                                                                                             cAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGGGATCCGACCTGGGGCGATGCGCTCCCCGACCGGCTGGTGCACAACGCTTATCGGACAGAACGGAAGGGCGAGTCGATGCGCAGGCGTGcg                                                 >  2:5516057/1‑151 (MQ=11)

                                                                                             cAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGcg                                                 <  1:1341652/151‑1 (MQ=11)

                                                                                             cAACCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGcg                                                 <  1:429652/151‑1 (MQ=11)

                                                                                              aaCCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGcga                                                >  2:5523394/1‑151 (MQ=11)

                                                                                               aCCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGcgac                                               <  1:2679585/151‑1 (MQ=11)

                                                                                               aCCGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGcgac                                               >  1:241417/1‑151 (MQ=11)

                                                                                                ccGCTCTACCCTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGGTCGAACTGGAGGGCGAATCGATGGGCGAGCGGGcgacg                                              >  2:5293142/1‑151 (MQ=11)

                                                                                                 cGCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGGGcgacga                                             >  2:5070377/1‑151 (MQ=11)

                                                                                                  gCTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGaa                                            <  1:1178153/151‑1 (MQ=14)

                                                                                                   cTCGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGaaa                                           <  2:4495085/151‑1 (MQ=14)

                                                                                                   cTCGACACTGGTGACCAGACAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGaaa                                           <  1:3155829/151‑1 (MQ=11)

                                                                                                     cGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAAtt                                         <  1:1669826/151‑1 (MQ=14)

                                                                                                     cGACACTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAAtt                                         <  1:5490497/151‑1 (MQ=14)

                                                                                                       acacTGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGa                                       <  2:6452297/151‑1 (MQ=14)

                                                                                                         acTGGTGACCAGCCAGATGCCGGTGGATAAATGGTATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGAcg                                     >  2:2513491/1‑151 (MQ=11)

                                                                                                           tGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGAcggc                                   <  1:3839118/151‑1 (MQ=14)

                                                                                                            ggTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGAcggcg                                  >  1:6247199/1‑151 (MQ=11)

                                                                                                            ggTGACCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGAcggcg                                  >  1:4694164/1‑151 (MQ=11)

                                                                                                              tgaCCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACggcggc                                >  1:3371996/3‑151 (MQ=14)

                                                                                                               gaCCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCa                               >  1:3211860/2‑151 (MQ=14)

                                                                                                               gaCCAGCCAGATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCa                               <  2:409835/150‑1 (MQ=14)

                                                                                                                 ccagccagATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAgg                             <  1:1398727/151‑1 (MQ=14)

                                                                                                                   agccagATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGa                           >  1:3542494/1‑151 (MQ=14)

                                                                                                                    gccagATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGAc                          >  1:4674778/1‑151 (MQ=14)

                                                                                                                    gccagATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGAc                          >  2:479304/1‑151 (MQ=14)

                                                                                                                     ccagATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGACt                         <  2:6075380/151‑1 (MQ=14)

                                                                                                                      cagATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCACGCGTGCGACGAAATTGACGGCGGCAGGGACtt                        >  1:4776839/1‑151 (MQ=14)

                                                                                                                       agATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGACTTc                       >  1:5925568/1‑151 (MQ=14)

                                                                                                                        gATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGACTTCa                      <  2:4214196/151‑1 (MQ=14)

                                                                                                                        gATGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGACTTCa                      >  2:1184690/1‑151 (MQ=14)

                                                                                                                         gtGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGACTTCAg                     <  2:127536/150‑1 (MQ=14)

                                                                                                                         aTGCCGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGACTTCAg                     >  1:5319462/1‑151 (MQ=14)

                                                                                                                            ccGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGACTTCAGACt                  <  1:6429987/151‑1 (MQ=14)

                                                                                                                             nggTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGACTTCAGACTa                 >  1:1190930/2‑151 (MQ=14)

                                                                                                                             cGGTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGACTTCAGACTa                 >  1:5067238/1‑151 (MQ=14)

                                                                                                                              ggTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGACTTCAGACTaa                <  1:2145188/151‑1 (MQ=14)

                                                                                                                              ggTGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGACTTCAGACTaa                <  1:6354143/151‑1 (MQ=14)

                                                                                                                                tGGATAAATGGCGTGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGACTTCAGACTAACa              <  2:5647076/151‑1 (MQ=14)

                                                                                                                                tGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGACTTCAGACTAACa              <  1:1864861/151‑1 (MQ=14)

                                                                                                                                tGGATAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGACTTCAGACTAACa              >  2:879401/1‑151 (MQ=14)

                                                                                                                                   atacacggCATGCACTGACGGGCGTTCCCACCTTGGGCGATGCGATCGTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGGAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGACTTCAGACTAACAATg           <  2:5152635/145‑1 (MQ=14)

                                                                                                                                      aaTGGCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGACTTCAGACTAACAATGCaa        >  2:2471409/1‑151 (MQ=255)

                                                                                                                                       aTGGCATGCACTGATCGGCGATCCGACCTTGGGCGTTGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGACTTCAGACTAACAATGCAAc       <  2:3907457/151‑1 (MQ=14)

                                                                                                                                        gggCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGACTTCAGACTAACAATGCAAcc      <  2:3965182/150‑1 (MQ=255)

                                                                                                                                         ggCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGACTTCAGACTAACAATGCAAccc     >  2:1921079/1‑151 (MQ=14)

                                                                                                                                         ggCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGACTTCAGACTAACAATGCAAccc     <  1:4135704/151‑1 (MQ=14)

                                                                                                                                          gCATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGACTTCAGACTAACAATGCAACCCt    <  1:3606727/151‑1 (MQ=255)

                                                                                                                                           cATGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGACTTCAGACTAACAATGCAACCCTg   <  1:19423/151‑1 (MQ=14)

                                                                                                                                           cATGCACTGACCGCCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAAGCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGACTTCAGACTAACAATGCAACCCTg   <  2:3846073/151‑1 (MQ=14)

                                                                                                                                            aTGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGACTTCAGACTAACAATGCAACCCTGc  >  1:4237245/1‑151 (MQ=255)

                                                                                                                                             tGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGACTTCAGACTAACAAt            >  2:4946749/1‑140 (MQ=14)

                                                                                                                                             tGCACTGATCGGCGATCCGACCTTGGGCGATGCGATCCTCGACCGGTTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGACTTCAGACTAACAAt            <  1:4946749/140‑1 (MQ=14)

GACTGACCTGCTGATCTTGGACGACTGGGGCCTGGCACCGTTTACCGCAGCGCAACGCCGCGACATGCTCGAACTGCTGGACGACCGCTACGGCAACCGCTCGACACTGGTCACCAGCCAGATGCCGGTGGACAAATGGCATGCGCTGATCGGCGATCCGACCTTGGGCGACGCGATCCTCGACCGTCTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAATCGATGCGCAAGCGTGCGACGAAATTGACGGCGGCAGGGACTTCAGACTAACAATGCAACCCTGC  >  CP009974/4957047‑4957337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`