Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 5,007,323 T→A F82Y (TTC→TAC)  RPPX_22185 → phenylacetate‑‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099745,007,3230TA99.1% 1129.4 / NA 320F82Y (TTC→TAC) RPPX_22185phenylacetate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  major base A (156/158);  minor base C (2/1);  total (158/162)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 320 total aligned reads displayed.

TGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTACCCCTTCACCGGCAAGAACGATCTGCGCGACAACTACCCCTTCGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGCGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGG  >  CP009974/5007175‑5007470
                                                                                                                                                    |                                                                                                                                                   
 ggAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACgg                                                                                                                                                  <  2:1117973/151‑1 (MQ=255)
    gCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCAt                                                                                                                                               <  1:5933558/151‑1 (MQ=255)
        gAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGttt                                                                                                                                           <  1:5383567/151‑1 (MQ=255)
          aGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGc                                                                                                                                         >  2:857466/1‑151 (MQ=255)
           gCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGcc                                                                                                                                        <  1:1157662/151‑1 (MQ=255)
            cACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGTCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGCTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCCACGGCACGTTTCCCg                                                                                                                                       >  2:1251329/1‑151 (MQ=255)
            cACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCg                                                                                                                                       <  2:101137/151‑1 (MQ=255)
             aCGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGt                                                                                                                                      >  2:2345067/1‑151 (MQ=255)
             aCGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGt                                                                                                                                      <  1:1549607/151‑1 (MQ=255)
             aCGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGt                                                                                                                                      >  1:847819/1‑151 (MQ=255)
               gCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGc                                                                                                                                    <  2:2086996/151‑1 (MQ=255)
                   aCGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCa                                                                                                                                <  2:3000956/151‑1 (MQ=255)
                       cAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGgaa                                                                                                                            >  1:5089181/1‑151 (MQ=255)
                       cAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGgaa                                                                                                                            >  2:659528/1‑151 (MQ=255)
                        aaTGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGgaag                                                                                                                           >  2:2688767/1‑151 (MQ=255)
                        aaTGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGgaag                                                                                                                           <  1:935770/151‑1 (MQ=255)
                         aTGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGgaaga                                                                                                                          <  1:2406222/151‑1 (MQ=255)
                         aTGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGgaaga                                                                                                                          <  2:3242765/151‑1 (MQ=255)
                                gTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAgtggtg                                                                                                                   <  1:5877202/151‑1 (MQ=255)
                                     aCCGGCAGCGCTTTGCCTAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCGCCTTCAGCGGCAAGAACGCCCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGAAAGAAGTGGTGCGCCt                                                                                                              <  2:5519166/151‑1 (MQ=255)
                                      ccGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTg                                                                                                             >  2:3827456/1‑151 (MQ=255)
                                          cAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATg                                                                                                         >  2:4185447/1‑151 (MQ=255)
                                              gcTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGGCGACCTTGCGAAGTTCCCCCTCACCGGCAAGAACGACCTGCGCGTCAACTACCCCTACGGCCTGCTGACCGTGTCCCAGGCAGACGTGGCCCGCCTGCACGCCTc                                                                                                     >  2:4508346/1‑151 (MQ=255)
                                              gcTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTc                                                                                                     <  2:1832284/151‑1 (MQ=255)
                                                  tGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAgt                                                                                                 <  2:6077549/151‑2 (MQ=255)
                                                  tGCCGAGTACGGCACCCACCCGGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAgt                                                                                                 <  2:3970949/151‑2 (MQ=255)
                                                     cGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGc                                                                                              <  2:5742303/151‑1 (MQ=255)
                                                     cGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGc                                                                                              <  1:370571/151‑1 (MQ=255)
                                                       aGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGcac                                                                                            >  1:4002083/1‑151 (MQ=255)
                                                         tACGGCACCCACCCTGGTGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGcacca                                                                                          <  1:577050/151‑1 (MQ=255)
                                                         tACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGcacca                                                                                          <  1:2824401/151‑1 (MQ=255)
                                                          aCGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGcaccac                                                                                         >  1:709932/1‑151 (MQ=255)
                                                            ggCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCg                                                                                       <  1:1320007/151‑1 (MQ=255)
                                                             gCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCgg                                                                                      >  1:835367/1‑151 (MQ=255)
                                                              cacccaccCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGc                                                                                     <  1:1252409/151‑1 (MQ=255)
                                                               acccacccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCa                                                                                    >  2:1742336/1‑151 (MQ=255)
                                                                cccacccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCaa                                                                                   <  1:4588021/151‑1 (MQ=255)
                                                                 ccacccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAg                                                                                  <  1:4397312/151‑1 (MQ=255)
                                                                 ccacccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAg                                                                                  <  1:6035462/151‑1 (MQ=255)
                                                                 ccacccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAg                                                                                  <  2:1525631/151‑1 (MQ=255)
                                                                 ccacccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGCCAAg                                                                                  <  2:5384936/151‑1 (MQ=255)
                                                                  cacccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGc                                                                                 <  2:5096663/151‑1 (MQ=255)
                                                                   acccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGcc                                                                                <  2:2165736/151‑1 (MQ=255)
                                                                    cccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCg                                                                               <  2:4349667/151‑1 (MQ=255)
                                                                       tgatgaCCTCAAATGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTGCGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACg                                                                            <  2:2595834/151‑1 (MQ=255)
                                                                       tgatgaCCGCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACg                                                                            <  2:6368890/151‑1 (MQ=255)
                                                                         atgaCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACggt                                                                          >  1:65409/1‑151 (MQ=255)
                                                                         atgaCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCCCCAGTGGCACCACCGGCCACCCGACggg                                                                          >  2:2852131/1‑150 (MQ=255)
                                                                          tgaCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACggtg                                                                         <  1:6505168/151‑1 (MQ=255)
                                                                          tgaCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCGTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTTCGGCATGTTTGCGGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACggtg                                                                         <  2:5662924/151‑1 (MQ=255)
                                                                            aCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACggtggt                                                                       <  1:2078361/151‑1 (MQ=255)
                                                                              cTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCg                                                                     >  1:526304/1‑151 (MQ=255)
                                                                              cTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCg                                                                     <  1:2992974/151‑1 (MQ=255)
                                                                                  aGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTa                                                                 <  1:6321624/151‑1 (MQ=255)
                                                                                   gTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTac                                                                >  1:2505257/1‑151 (MQ=255)
                                                                                    tGCCTGGACGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACACCTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTacc                                                               >  1:1974867/1‑150 (MQ=255)
                                                                                    tGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTaca                                                               >  1:796962/1‑151 (MQ=255)
                                                                                     gCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTacac                                                              >  1:4750702/1‑151 (MQ=255)
                                                                                      ccTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACAcc                                                             <  1:1050663/151‑1 (MQ=255)
                                                                                       cTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACAccc                                                            <  2:1169137/151‑1 (MQ=255)
                                                                                       cTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACAccc                                                            <  1:5545659/151‑1 (MQ=255)
                                                                                        tGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCa                                                           >  1:4864002/1‑151 (MQ=255)
                                                                                        tGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCGGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGGGCGCCTGCATGGCGCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACAcccg                                                           >  2:3409550/1‑150 (MQ=255)
                                                                                            aGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGTCCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAAc                                                       <  2:3613142/151‑1 (MQ=255)
                                                                                              aCCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCCAGCCGACGGTGGGCGGCTACACCCAGAACGa                                                     >  2:134018/1‑151 (MQ=255)
                                                                                                     gAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGac                                              >  1:2656904/1‑151 (MQ=255)
                                                                                                      aaGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGaca                                             >  2:4744954/1‑151 (MQ=255)
                                                                                                      aaGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGaca                                             >  1:3363106/1‑151 (MQ=255)
                                                                                                       aGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGacac                                            <  1:4806440/151‑1 (MQ=255)
                                                                                                         ttccccTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGCACGATATCGACACCt                                          >  2:2452874/3‑151 (MQ=255)
                                                                                                         ttccccTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCt                                          <  2:5313399/149‑1 (MQ=255)
                                                                                                              cTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCGGTGCTCCAGGAAGAAGTGGTGCTCCTGCATGTCTTCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGGTACACCCAGACCGATATCGACACCTGGGcc                                     >  1:2751689/1‑151 (MQ=255)
                                                                                                               ttCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGccc                                    >  2:5180587/1‑150 (MQ=255)
                                                                                                                tCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCaa                                   >  1:3449742/1‑151 (MQ=255)
                                                                                                                tCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCaa                                   <  1:3817650/151‑1 (MQ=255)
                                                                                                                   ccGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAAtgt                                >  1:2169807/1‑151 (MQ=255)
                                                                                                                    cGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAAtgtg                               <  1:3255391/151‑1 (MQ=255)
                                                                                                                    cGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAAtgtg                               <  1:5717840/151‑1 (MQ=255)
                                                                                                                    cGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAAtgtg                               <  1:1779008/151‑1 (MQ=255)
                                                                                                                     ggCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTgg                              <  2:835367/151‑1 (MQ=255)
                                                                                                                     ggCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTgg                              >  1:3498607/1‑151 (MQ=255)
                                                                                                                        aaGAACGACCTGCGCGACAACGACCCCGACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTg                           <  2:1851760/151‑1 (MQ=255)
                                                                                                                          gAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGcc                         <  2:5840782/151‑1 (MQ=255)
                                                                                                                            aCGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCg                       <  1:4068385/151‑1 (MQ=255)
                                                                                                                            aCGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCg                       <  2:3652735/151‑1 (MQ=255)
                                                                                                                             cgaccTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGt                      <  1:6240778/147‑1 (MQ=255)
                                                                                                                              gaccTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGCACGATATCGACACCTGGGCCAATGTGGTTGCCCGtt                     >  2:1917012/4‑151 (MQ=255)
                                                                                                                               accTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTc                    >  2:3126498/3‑151 (MQ=255)
                                                                                                                                  tGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCAt                 >  1:6018861/1‑151 (MQ=255)
                                                                                                                                      cgACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGACCTCGACACCTGCGCCAAGGCGGCCGCCCGCTCCCTTccc             >  2:4798308/1‑149 (MQ=255)
                                                                                                                                       gACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTg            <  2:754605/151‑1 (MQ=255)
                                                                                                                                       gACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTCCCCTCCGtc            >  2:4453138/1‑150 (MQ=255)
                                                                                                                                        aCAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGc           <  1:3972423/151‑1 (MQ=255)
                                                                                                                                           aCTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGcggc        >  2:3453372/1‑151 (MQ=255)
                                                                                                                                             tACCCCTCCGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGcggccg      <  1:5237522/151‑1 (MQ=255)
                                                                                                                                             tACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGcggccg      >  2:3076330/1‑151 (MQ=255)
                                                                                                                                             tACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGcggccc      >  2:4228842/1‑150 (MQ=255)
                                                                                                                                             tACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGcgcccc      >  2:4268029/1‑150 (MQ=255)
                                                                                                                                               ccccTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATCTCGACACCTGGGCCAATGTGGTTGCCCGTCCCATTCGTGcggccggc    >  2:5957291/1‑151 (MQ=255)
                                                                                                                                                cccTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCcggcg   >  1:1684096/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTACCCCTTCACCGGCAAGAACGATCTGCGCGACAACTACCCCTTCGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGCGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGG  >  CP009974/5007175‑5007470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑