Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 5,007,375 C→T S99S (AGC→AGT)  RPPX_22185 → phenylacetate‑‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099745,007,3750CT99.4% 1140.5 / NA 311S99S (AGC→AGT) RPPX_22185phenylacetate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (151/157);  minor base G (0/2);  total (152/159)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 311 total aligned reads displayed.

CCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTACCCCTTCACCGGCAAGAACGATCTGCGCGACAACTACCCCTTCGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGCGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCCTACGGCTACGGGCTGTTCACCGGC  >  CP009974/5007227‑5007522
                                                                                                                                                    |                                                                                                                                                   
ccGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTgg                                                                                                                                                   <  2:3148713/151‑1 (MQ=255)
   aGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGTAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGcac                                                                                                                                                <  2:5763836/151‑1 (MQ=255)
   aGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGcac                                                                                                                                                >  1:4002083/1‑151 (MQ=255)
      aCGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGcaccac                                                                                                                                             >  1:709932/1‑151 (MQ=255)
        ggCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCg                                                                                                                                           <  1:1320007/151‑1 (MQ=255)
          cacccaccCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGc                                                                                                                                         <  1:1252409/151‑1 (MQ=255)
          cacccaccCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGc                                                                                                                                         >  2:4286883/1‑151 (MQ=255)
           ccccacccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCa                                                                                                                                        <  1:945131/150‑1 (MQ=255)
            cccacccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCaa                                                                                                                                       <  1:4588021/151‑1 (MQ=255)
             ccacccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAg                                                                                                                                      <  1:4397312/151‑1 (MQ=255)
             ccacccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAg                                                                                                                                      >  1:5257762/1‑151 (MQ=255)
             ccacccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAg                                                                                                                                      <  1:6035462/151‑1 (MQ=255)
              cacccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGc                                                                                                                                     <  1:5036460/151‑1 (MQ=255)
                cccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCg                                                                                                                                   >  1:2225009/1‑151 (MQ=255)
                cccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCg                                                                                                                                   <  2:4349667/151‑1 (MQ=255)
                cccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCg                                                                                                                                   >  1:2064410/1‑151 (MQ=255)
                 ccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGa                                                                                                                                  <  1:4974729/151‑1 (MQ=255)
                  cTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGAc                                                                                                                                 <  1:2865208/151‑1 (MQ=255)
                   tgatgaCCTCAAATGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTGCGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACg                                                                                                                                <  2:2595834/151‑1 (MQ=255)
                       gaCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACggtgg                                                                                                                            >  2:5423060/1‑151 (MQ=255)
                        aCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACggtggt                                                                                                                           <  1:2078983/151‑1 (MQ=255)
                        aCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACggtggt                                                                                                                           >  1:6258639/1‑151 (MQ=255)
                         ccTCAAGTGCCTGGAAGACCTGGCGAAGTTCGCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTc                                                                                                                          <  2:2719503/151‑1 (MQ=255)
                         ccTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTc                                                                                                                          >  1:3685206/1‑151 (MQ=255)
                          cTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCg                                                                                                                         <  1:2992974/151‑1 (MQ=255)
                          cTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCg                                                                                                                         >  2:813799/1‑151 (MQ=255)
                           tCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCgg                                                                                                                        >  1:814448/1‑151 (MQ=255)
                            cAAGTGCCGGGAGGACCTCGCGACGTGCCCCTTCACCGGCAAGACCGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGc                                                                                                                       <  2:4580817/151‑1 (MQ=255)
                             aaGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGTGGGTCGGCt                                                                                                                      >  2:5133560/1‑151 (MQ=255)
                               gTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTac                                                                                                                    >  2:879563/1‑151 (MQ=255)
                               gTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTac                                                                                                                    >  1:2505257/1‑151 (MQ=255)
                               gTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGCAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTac                                                                                                                    <  1:3103840/151‑1 (MQ=255)
                                tGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTaca                                                                                                                   >  1:796962/1‑151 (MQ=255)
                                  ccTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACAcc                                                                                                                 <  1:1050663/151‑1 (MQ=255)
                                  ccTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACAcc                                                                                                                 >  2:5623232/1‑151 (MQ=255)
                                   cTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACAccc                                                                                                                <  1:5545659/151‑1 (MQ=255)
                                     ggAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAg                                                                                                              <  1:5890289/151‑1 (MQ=255)
                                      gAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGa                                                                                                             <  1:3316153/151‑1 (MQ=255)
                                       aaGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCACGAAGAAGTGTTGCGCCTCCATGCCCCCAGTGGCACCACCGGCCAGCCGCCGGTGGCCGCCTACAccccgcc                                                                                                            >  2:4186254/1‑147 (MQ=255)
                                        aGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGTCCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAAc                                                                                                           <  2:3613142/151‑1 (MQ=255)
                                             tGGCGAAGTTCCCCTTCACCGGCAAGACCGACCTCCGCGACAACCACCCCTACGGCCTGTTTGCCGTGCCCCCGGAAGAAGCCGTGCGCCTGCCCGCCTCCAGCGGCACCACCGGCAAGCCGACCGCGGCCGGCTCCACCCCGCACGacat                                                                                                      >  2:1236304/1‑151 (MQ=255)
                                             tGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGatat                                                                                                      <  1:2805689/151‑1 (MQ=255)
                                                 gAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCACGCCGCCGGTGGTCGGCTACACCCAGAACGGTATCGac                                                                                                  >  2:4239791/1‑151 (MQ=255)
                                                 gAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGac                                                                                                  >  1:2656904/1‑151 (MQ=255)
                                                  aaGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGaca                                                                                                 <  1:6512105/151‑1 (MQ=255)
                                                  aaGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGaca                                                                                                 >  2:4744954/1‑151 (MQ=255)
                                                   aGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCGCCGGCAAGCCGACGGTGGTCGGCTCCACCCAGCACGATATCGacac                                                                                                >  2:5722956/1‑151 (MQ=255)
                                                   aGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGacac                                                                                                <  1:4806440/151‑1 (MQ=255)
                                                   aGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGacac                                                                                                >  1:5950838/1‑151 (MQ=255)
                                                     ttccccTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCt                                                                                              <  2:5313399/149‑1 (MQ=255)
                                                       ccccTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGCCGGCTACACCCAGACCGATATCGACACCTgg                                                                                            >  2:4922189/1‑151 (MQ=255)
                                                                cGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAAtgtg                                                                                   <  1:1779008/151‑1 (MQ=255)
                                                                cGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAAtgtg                                                                                   <  1:5717840/151‑1 (MQ=255)
                                                                    aaGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTg                                                                               <  2:5950838/151‑1 (MQ=255)
                                                                      gAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGcc                                                                             >  2:2856040/1‑151 (MQ=255)
                                                                       aaCGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGccc                                                                            <  1:3461656/151‑1 (MQ=255)
                                                                        aCGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCg                                                                           <  1:4068385/151‑1 (MQ=255)
                                                                          gaccTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGCACGATATCGACACCTGGGCCAATGTGGTTGCCCGtt                                                                         >  2:1917012/4‑151 (MQ=255)
                                                                          gaccTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGtt                                                                         >  2:1895329/4‑151 (MQ=255)
                                                                                  cgACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGt                                                                 >  2:1770134/1‑151 (MQ=255)
                                                                                   naCAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTg                                                                >  1:5768287/2‑151 (MQ=255)
                                                                                   gACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTg                                                                <  2:847767/151‑1 (MQ=255)
                                                                                    aCAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGc                                                               >  2:6169501/1‑151 (MQ=255)
                                                                                    aCAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGc                                                               <  1:3972423/151‑1 (MQ=255)
                                                                                       aCTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGcggc                                                            >  2:3453372/1‑151 (MQ=255)
                                                                                       aCTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTAGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGcggc                                                            >  2:1841008/1‑151 (MQ=255)
                                                                                         tACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGcggccg                                                          <  1:5341019/151‑1 (MQ=255)
                                                                                         tACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGcggccg                                                          >  2:3076330/1‑151 (MQ=255)
                                                                                            cccTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCcggcg                                                       >  1:1684096/1‑151 (MQ=255)
                                                                                             ccTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCCTTCGTGCGCCcgccgg                                                      >  2:6501420/1‑151 (MQ=255)
                                                                                                    cATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCaaa                                               >  1:1368657/1‑151 (MQ=255)
                                                                                                    cATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCaaa                                               >  2:2399751/1‑151 (MQ=255)
                                                                                                      tGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAgg                                             <  1:3711544/151‑1 (MQ=255)
                                                                                                      tGTTTGCCGTGCCCCAGGAAGAAGTCGTGCGCCTGCATGCCCCAAGTGGCACTACCGGCTAGCCGCCGGCGGTCGGCTACCCCCAGAACGATACCGACACCTGGGCCAATGTGGTTGCACGTTCCATACGTGCGGCCGGCGGGCGCAAAgg                                             >  2:168709/1‑151 (MQ=255)
                                                                                                       gTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGt                                            <  1:6169501/151‑1 (MQ=255)
                                                                                                        tttGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTg                                           <  1:2425383/151‑1 (MQ=255)
                                                                                                         ttGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGa                                          <  2:6031688/151‑1 (MQ=255)
                                                                                                           gCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACa                                        >  2:1109149/1‑151 (MQ=255)
                                                                                                            ccGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACaa                                       <  1:4744954/151‑1 (MQ=255)
                                                                                                               tGCACCAGGAAGAAGTGGTGCGCCTGCATGCCTCAAGTGGGACCACCGGAAAGCTGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGt                                    <  2:633500/151‑1 (MQ=255)
                                                                                                                 ccccAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGc                                  <  2:3745608/151‑1 (MQ=255)
                                                                                                                  cccAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCACAGGTGACAAGGTGCa                                 >  2:1731417/1‑151 (MQ=255)
                                                                                                                  cccAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCa                                 <  2:608583/151‑1 (MQ=255)
                                                                                                                     aGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCAtgt                              >  2:4010200/1‑151 (MQ=255)
                                                                                                                          gaagTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCct                         <  1:1853344/151‑1 (MQ=255)
                                                                                                                          gaagTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCct                         <  1:4840032/151‑1 (MQ=255)
                                                                                                                            agTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCctac                       <  2:3802775/151‑1 (MQ=255)
                                                                                                                             gtggtgCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCctacg                      <  2:1054531/151‑1 (MQ=255)
                                                                                                                             gtggtgCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCctacg                      <  1:2553342/151‑1 (MQ=255)
                                                                                                                                gtgCGCCTGCATGCCGCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCctacggct                   <  2:3669815/151‑1 (MQ=255)
                                                                                                                                 tgCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCctacggcta                  <  1:879563/151‑1 (MQ=255)
                                                                                                                                   cgcCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCctacggctacg                >  2:1515189/1‑151 (MQ=255)
                                                                                                                                           tGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCCTACGGCTACGGGCTGTTc        <  1:3373859/151‑1 (MQ=255)
                                                                                                                                           tGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTCCATGTGTCCTCCGGCTACGCGCTGCTc        >  2:3257547/1‑151 (MQ=255)
                                                                                                                                           tGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGACGCCAAGGTGACAAGGTGCATGTGTCCTACCGCCACGGGCTGCTc        >  2:5041973/1‑151 (MQ=255)
                                                                                                                                            gCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCCTACGGCTACGGGCTGTTCa       <  1:6326440/151‑1 (MQ=255)
                                                                                                                                             ccTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCCTACGGCTACGGGCTGTTCAc      <  2:2082149/151‑1 (MQ=255)
                                                                                                                                               cccAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCATAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCCTACGGCTACGGGCTGTTCACcg    <  2:3429589/150‑1 (MQ=255)
                                                                                                                                                ccAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCCTACGGCTACGGGCTGTTCACcgg   >  1:1587785/1‑151 (MQ=255)
                                                                                                                                                 cAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCCTACGGCTACGGGCTGTTCACcggc  >  1:3824046/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTACCCCTTCACCGGCAAGAACGATCTGCGCGACAACTACCCCTTCGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGCGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCCTACGGCTACGGGCTGTTCACCGGC  >  CP009974/5007227‑5007522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑