Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 659,171 A→G V185A (GTC→GCC)  RPPX_02840 ← ribonuclease D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009974659,1710AG99.7% 1226.8 / NA 344V185A (GTC→GCC) RPPX_02840ribonuclease D
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (188/155);  minor base T (1/0);  total (189/155)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 344 total aligned reads displayed.

CACATCGCGGTTGCGCGCTTCGCGCTCGCGCCAGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGACGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCTGACCTGGGTTTCCGAGAGCGGGCGTTGCAGCCAGTCGGAGCGGGTTT  >  CP009974/659023‑659318
                                                                                                                                                    |                                                                                                                                                   
cacaTCGCGGTTGCGCGCTTCGCGCTCGCGCCAGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCg                                                                                                                                                   <  2:4296948/151‑1 (MQ=255)
  caTCGCGGTTGCGCGCTTCGCGCTCGCGCCAGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGcc                                                                                                                                                 >  1:277816/1‑151 (MQ=255)
   aTCGCGGTTGCGCGCTTCGCGCTCGCGCCAGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCg                                                                                                                                                >  2:1069319/1‑151 (MQ=255)
    tCGCGGTTGCGCGCTTCGCGCTCGCGCCAGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGt                                                                                                                                               >  2:1443893/1‑151 (MQ=255)
    tCGCGGTTGCGCGCTTCGCGCTCGCGCCAGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGt                                                                                                                                               >  2:3052282/1‑151 (MQ=255)
        ggTTGCGCGCTTCGCGCTCGCGCCAGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTc                                                                                                                                           >  2:1967034/1‑151 (MQ=255)
        ggTTGCGCGCTTCGCGCTCGCGCCAGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTc                                                                                                                                           <  2:5761467/151‑1 (MQ=255)
         gTTGCGCGCTTCGCGCTCGCGCCAGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTcc                                                                                                                                          <  1:4422834/151‑1 (MQ=255)
         gTTGCGCGCTTCGCGCTCGCGCCAGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTcc                                                                                                                                          >  2:387342/1‑151 (MQ=255)
          ttGCGCGCTTCGCGCTCGCGCCAGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTccc                                                                                                                                         >  1:3621229/1‑150 (MQ=255)
          ttGCGCGCTTCGCGCTCGCGCCAGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCa                                                                                                                                         >  1:6414742/1‑151 (MQ=255)
           tGCGCGCTTCGCGCTCGCGCCAGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAg                                                                                                                                        <  1:524299/151‑1 (MQ=255)
           tGCGCGCCGCGCGCGCGCGCCAGGCGCACAGCGCACGTAGCACGGCCAGTTGTGGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAg                                                                                                                                        <  2:6220897/151‑1 (MQ=255)
            gcgcgcTTCGCGCTCGCGCCAGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGc                                                                                                                                       <  1:2888090/151‑1 (MQ=255)
                gcTGCGCGCTCGCGCCAGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCAccc                                                                                                                                   <  2:2992045/151‑1 (MQ=255)
                 cTTCGCGCTCGCGCCAGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCa                                                                                                                                  >  1:5410208/1‑151 (MQ=255)
                  ttCGCGCTCGCGCCAGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAg                                                                                                                                 <  1:4048125/151‑1 (MQ=255)
                  ttCGCGCTCGCGCCAGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCGCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAg                                                                                                                                 >  2:5899701/1‑151 (MQ=255)
                    cgcgctcgcgcCAGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGc                                                                                                                               <  1:2968012/151‑1 (MQ=255)
                     gcgctcgcgcCAGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCa                                                                                                                              >  2:4869925/1‑151 (MQ=255)
                     gcgctcgcgcCAGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTGTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCa                                                                                                                              >  1:89300/1‑151 (MQ=255)
                       gctcgcgcCAGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATa                                                                                                                            >  2:286791/1‑151 (MQ=255)
                        ctcgcgcCAGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATAc                                                                                                                           >  2:2712582/1‑151 (MQ=255)
                        ctcgcgcCAGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATAc                                                                                                                           >  1:1518090/1‑151 (MQ=255)
                             gcCAGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTgtc                                                                                                                      <  2:2138039/151‑1 (MQ=255)
                             gcCAGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTgtc                                                                                                                      <  2:3275257/151‑1 (MQ=255)
                                gggCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTgtcgtc                                                                                                                   <  2:3620968/150‑1 (MQ=255)
                                aGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTgtcgtc                                                                                                                   >  1:3486276/1‑151 (MQ=255)
                                aGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTgtcgtc                                                                                                                   >  1:1942688/1‑151 (MQ=255)
                                 ggCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCa                                                                                                                  >  1:1270630/1‑151 (MQ=255)
                                  gcacacgGCTCACGTAGCACTGCCAGTTGTTGAGGCTCCAGCTGCCGGTAAAGCTTGACGTCGCGGTAACGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCAc                                                                                                                 <  1:3826245/148‑1 (MQ=255)
                                   cgcACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACt                                                                                                                <  2:4736142/151‑1 (MQ=255)
                                    gcACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTc                                                                                                               >  1:2757325/1‑151 (MQ=255)
                                    gcACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTc                                                                                                               >  1:2264140/1‑151 (MQ=255)
                                     cacaGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCa                                                                                                              <  1:2092998/151‑1 (MQ=255)
                                       caGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAAc                                                                                                            >  1:5848164/1‑151 (MQ=255)
                                        aGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAAcc                                                                                                           >  1:974375/1‑151 (MQ=255)
                                        aGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAAcc                                                                                                           >  1:3037588/1‑151 (MQ=255)
                                        aGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAAcc                                                                                                           <  1:5497125/151‑1 (MQ=255)
                                        aGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAAcc                                                                                                           >  1:6070512/1‑151 (MQ=255)
                                          cTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACcgc                                                                                                         <  1:210074/151‑1 (MQ=255)
                                          cTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACcgc                                                                                                         >  2:1620653/1‑151 (MQ=255)
                                           tCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACcgcg                                                                                                        >  1:7963/1‑151 (MQ=255)
                                            cACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCCGCACCCAGGCATACTTGTCGTCACTCAACCGCgg                                                                                                       >  1:1137257/1‑151 (MQ=255)
                                                  gCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACgcag                                                                                                 <  2:1873177/151‑1 (MQ=255)
                                                  gCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACgcag                                                                                                 >  1:4769909/1‑151 (MQ=255)
                                                   cACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACgcagc                                                                                                >  1:1032683/1‑151 (MQ=255)
                                                            ttgttgAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGTCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAAc                                                                                       <  2:5029010/151‑1 (MQ=255)
                                                            ttgttgAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAAc                                                                                       <  2:1750300/151‑1 (MQ=255)
                                                              gttgAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCCGCACAg                                                                                     >  2:1686173/1‑151 (MQ=255)
                                                                 gAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTc                                                                                  <  2:1400703/151‑1 (MQ=255)
                                                                   ggCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCgg                                                                                <  2:6008524/151‑1 (MQ=255)
                                                                    gcgcCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACCCAGCACGCCGAACAGCTCgcc                                                                               >  2:4245934/1‑149 (MQ=255)
                                                                       ccAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAg                                                                            >  1:5304023/1‑151 (MQ=255)
                                                                       ccAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGAGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACTGCGGACGCAGCACGCTGAACAGCTCGGCCAg                                                                            >  2:5121867/1‑151 (MQ=255)
                                                                         aGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGt                                                                          <  1:370426/151‑1 (MQ=255)
                                                                           cTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGc                                                                        <  1:5763101/151‑1 (MQ=255)
                                                                            ttCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCCCGCTGAACCGCTCCGCCAGGTGcc                                                                       >  2:2379212/1‑150 (MQ=255)
                                                                            ttCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCa                                                                       >  1:5032144/1‑151 (MQ=255)
                                                                            ttCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCa                                                                       <  1:3099235/151‑1 (MQ=255)
                                                                                 ggCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCa                                                                  <  2:6382707/151‑1 (MQ=255)
                                                                                  gCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCAt                                                                 >  1:5174357/1‑151 (MQ=255)
                                                                                   ccAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACCGCATc                                                                >  2:4168708/1‑151 (MQ=255)
                                                                                     aGCTTGACGTAGCGGTAAAGGCTTTCAGGCTAGACTTCACGGCGAAACGCAGCCACCCGCTCGGCGCCGTATTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCtt                                                              <  2:2547490/151‑1 (MQ=255)
                                                                                       cTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCg                                                            >  2:937741/1‑151 (MQ=255)
                                                                                            ggTCGCGGTAAAGGCTTTCAGGCGCGACTTCACGGCGCAACGCAGCCACCAGCGCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGc                                                       <  2:5401738/150‑1 (MQ=255)
                                                                                            cGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGc                                                       <  2:4073458/151‑1 (MQ=255)
                                                                                                  ggTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCt                                                 <  2:3486276/151‑1 (MQ=255)
                                                                                                  ggTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCt                                                 >  1:333345/1‑151 (MQ=255)
                                                                                                  ggTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCCTCGGCAGCGTAGCt                                                 >  2:5861419/1‑151 (MQ=255)
                                                                                                       aGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCCTCCCGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCCGGTGCCCGGCACCTTCGCCACCGTAGCTGAccc                                            >  2:2671231/1‑150 (MQ=255)
                                                                                                          cTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCTGACCTggg                                         >  1:2300606/1‑151 (MQ=255)
                                                                                                            ttCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGACCAGCCCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCCGACCCGGGtt                                       >  2:244045/1‑151 (MQ=255)
                                                                                                            ttCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCTGACCTGGGtt                                       <  1:6339055/151‑1 (MQ=255)
                                                                                                             tCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCTGACCTGGGttt                                      >  2:317488/1‑151 (MQ=255)
                                                                                                             tCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCTGACCTGGGttt                                      <  2:4611440/151‑1 (MQ=255)
                                                                                                              cAGGCTCGACTTCACGGCGCAACGCACCCACCCGCTCGGCGCCGTCTTCCATCACCCACGCGTACTTGTCGACCCCCAAGCGCGGCACCAGCACGCTGCACAGCTCGGCGCTGTCCCCCACACCGTCCGCCGCGTacctgacctgcacttc                                     >  2:5616920/1‑145 (MQ=255)
                                                                                                                ggCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCTGACCTGGGGTTCCg                                   >  1:3574556/1‑151 (MQ=255)
                                                                                                                 gCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCTGACCTGGGTTTCCgc                                  >  2:615123/1‑150 (MQ=255)
                                                                                                                   tCGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCTGACCTGGGTTTCCgaga                                <  2:225637/151‑1 (MQ=255)
                                                                                                                    cGACTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCTGACCTGGGTTTCCGagag                               <  1:2552602/151‑1 (MQ=255)
                                                                                                                      aCTTCACGGCGCCACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCTGACCTGGGTTTCCGAGAGCg                             <  2:743068/151‑1 (MQ=255)
                                                                                                                       cTTCACGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCTGACCTGGGTTTCCGAGAGCgg                            <  1:495655/151‑1 (MQ=255)
                                                                                                                           aCGGCGCAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTGGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCTGACCTGGGTTTCCGAGAGCGGGCGt                        <  2:3958302/151‑1 (MQ=255)
                                                                                                                              gcgcAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCTGACCTGGGTTTCCGAGAGCGGGCGTTGc                     >  2:4464687/1‑151 (MQ=255)
                                                                                                                              gcgcAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCTGACCTGGGTTTCCGAGAGCGGGCGTTGc                     >  1:4240144/1‑151 (MQ=255)
                                                                                                                               cgcAACGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCTGACCTGGGTTTCCGAGAGCGGGCGTTGCa                    <  1:71818/151‑1 (MQ=255)
                                                                                                                                  aaCGCAGCCACCAGATCGGCGCCGTCTTTTAGCACCCATCCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGATGACTTGGGTTTCCGGGAGGGGGCGTGGCAGcc                 >  2:1176090/1‑151 (MQ=255)
                                                                                                                                    cGCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCTGACCTGGGTTTCCGAGAGCGGGCGTTGCAGCCAg               <  2:4922735/151‑1 (MQ=255)
                                                                                                                                     gCAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCTGACCTGGGTTTCCGAGAGCGGGCGTTGCAGCCAGt              <  1:4431641/151‑1 (MQ=255)
                                                                                                                                      cAGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCTGACCTGGGTTTCCGAGAGCGGGCGTTGCAGCCAGTc             <  1:1854664/151‑1 (MQ=255)
                                                                                                                                       aGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCTGACCTGGGTTTCCGAGAGCGGGCGTTGCAGCCAGTCg            <  1:2403372/151‑1 (MQ=255)
                                                                                                                                       aGCCACCAGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCTGACCTGGGTTTCCGAGAGCGGGCGTTCCAGCCAGTCg            >  2:5665491/1‑151 (MQ=255)
                                                                                                                                         ccaccaGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCTGACCTGGGTTTCCGAGAGCGGGCGTTGCAGCCAGTCGGa          >  2:3391598/1‑151 (MQ=255)
                                                                                                                                         ccaccaGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCTGACCTGGGTTTCCGAGAGCGGGCGTTGCAGCCAGTCGGa          <  1:1367313/151‑1 (MQ=255)
                                                                                                                                            ccaGCTCGGCGCCGTCGTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCTGACCTGGGTTTCCGAGAGCGGGCGTTGCAGCCAGTCGGAGCg       <  2:5097139/151‑1 (MQ=255)
                                                                                                                                              aGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGCACAGCCCGGCCAGGTGCCCGGCATCTTCGGCAGCGTAGCTGACCTGGGTGTCCGAGAGCGGGCGTTGCAGCCAGTCGGAGCgcg     >  2:3523569/1‑149 (MQ=255)
                                                                                                                                              aGCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCTGACCTGGGTTTCCGAGAGCGGGCGTTGCAGCCAGTCGGAGCggg     >  2:1461102/1‑151 (MQ=255)
                                                                                                                                               gCTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCTGACCTGGGTTTCCGAGAGCGGGCGTTGCAGCCAGTCGGAGCGGGt    <  1:2837963/151‑1 (MQ=255)
                                                                                                                                                cTCGGCGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCTGACCTGGGTTTCCGAGAGCGGGCGTTGCAGCCAGTCGGAGCGGGtt   <  1:1777889/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CACATCGCGGTTGCGCGCTTCGCGCTCGCGCCAGGCGCACAGCTCACGTAGCACTGCCAGTTGTTGAGGCGCCAGCTTCCAGGCCAGCTTGACGTCGCGGTAAAGGCTTTCAGGCTCGACTTCACGGCGCAACGCAGCCACCAGCTCGACGCCGTCTTCCAGCACCCAGGCATACTTGTCGTCACTCAACCGCGGACGCAGCACGCTGAACAGCTCGGCCAGGTGCACGGCATCTTCGGCAGCGTAGCTGACCTGGGTTTCCGAGAGCGGGCGTTGCAGCCAGTCGGAGCGGGTTT  >  CP009974/659023‑659318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑