BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	5,105,402	G→A	F150F (TTC→TTT)	RPPX_22650 ←	benzene 1,2‑dioxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	5,105,402	0	G	A	94.4%	371.1 / 11.4	195	F150F (TTC→TTT)	RPPX_22650	benzene 1,2‑dioxygenase
Reads supporting (aligned to +/- strand): ref base G (6/5); new base A (87/97); total (93/102)
Fisher's exact test for biased strand distribution p-value = 7.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 195 total aligned reads displayed.

GGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGTGGAACGGGCAGGTATAGCTGGAACGGTTG  >  CP009974/5105264‑5105549

agctccacgcAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCCGTGGTTTCACATCGGCCTTCAGACTGCCGAACAGGAAGCCCCGGTACGACTCGAATCGTgccgcc                                                                                                                                         >  1:5408972/8‑151 (MQ=1)

  ctccacgcAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGCTACGACTCGAATCGTGCCACCtt                                                                                                                                       >  2:1824337/6‑151 (MQ=1)

   tccacgcAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTg                                                                                                                                      >  1:1811720/5‑151 (MQ=1)

           ccactcgcAGGCCTTCAGCCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTc                                                                                                                              <  2:4718383/144‑1 (MQ=1)

                         tccggCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGc                                                                                                                >  1:362111/4‑151 (MQ=1)

                                 cTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAg                                                                                                        >  1:4310440/1‑151 (MQ=11)

                                 cTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGCCGCAGTTGAAg                                                                                                        >  2:3637124/1‑151 (MQ=11)

                                  tGATCGACGATCATGTCGATGATCTTCGCCGGCTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGc                                                                                                       <  1:2386899/151‑1 (MQ=11)

                                  tGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGc                                                                                                       >  1:4449343/1‑151 (MQ=11)

                                   gATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCt                                                                                                      >  2:5339482/1‑151 (MQ=11)

                                    aTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTg                                                                                                     <  2:5498016/151‑1 (MQ=11)

                                    aTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTg                                                                                                     <  2:4447356/151‑1 (MQ=11)

                                     gcgacgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGt                                                                                                    <  2:5641988/150‑1 (MQ=11)

                                      cgacgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTc                                                                                                   >  2:2534557/1‑151 (MQ=11)

                                       gacgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCg                                                                                                  <  1:5048586/151‑1 (MQ=11)

                                        acgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCgg                                                                                                 >  2:2882316/1‑151 (MQ=11)

                                        acgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCgg                                                                                                 <  1:1019059/151‑1 (MQ=11)

                                         cgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCggg                                                                                                <  1:5396839/151‑1 (MQ=11)

                                          gaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCgggg                                                                                               >  1:3883702/1‑151 (MQ=11)

                                           aTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGt                                                                                              <  1:2078851/151‑1 (MQ=11)

                                           aTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGt                                                                                              >  2:2407035/1‑151 (MQ=11)

                                            tCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTa                                                                                             >  2:2559549/1‑151 (MQ=11)

                                              aTGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGc                                                                                           <  1:127138/151‑1 (MQ=11)

                                               tGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGcc                                                                                          <  1:2805323/151‑1 (MQ=11)

                                                gTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCg                                                                                         >  1:856731/1‑151 (MQ=11)

                                                gTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCg                                                                                         <  2:4870137/151‑1 (MQ=11)

                                                gTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCg                                                                                         <  1:4975283/151‑1 (MQ=11)

                                                  cGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGc                                                                                       <  1:3285413/151‑1 (MQ=11)

                                                    atgatCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGt                                                                                     <  2:1655002/151‑1 (MQ=11)

                                                    atgatCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGCGGGAGCCGTCGCCGCCGAAGCCGCCGGGGTAGGCGGCGt                                                                                     >  2:1334316/1‑151 (MQ=11)

                                                      gatCTTCGCCGACTCGCCCAGGTGCTCGGCCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTg                                                                                   <  2:4123285/151‑1 (MQ=11)

                                                      gatCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTg                                                                                   >  1:735301/1‑151 (MQ=11)

                                                       atCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGc                                                                                  <  1:1212443/151‑1 (MQ=11)

                                                         cTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTg                                                                                >  1:2950263/1‑151 (MQ=11)

                                                         cTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTg                                                                                <  1:3270314/151‑1 (MQ=11)

                                                          ttCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTgg                                                                               <  2:856731/151‑1 (MQ=11)

                                                          ttCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTgg                                                                               <  1:1865237/151‑1 (MQ=11)

                                                            cGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTgggg                                                                             <  1:6207157/151‑1 (MQ=11)

                                                             gCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGt                                                                            <  1:4328350/151‑1 (MQ=11)

                                                              ccGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTc                                                                           <  2:512554/151‑1 (MQ=11)

                                                               gggcgcGCCCGGGTGCTCGACGAGAGGCTTGCCCTCGGCGTTTGGGCAGCCGAACAAGAGGCCCCGGTACGACTCAAAACGTGCCCCCTTGGTCGGGTCGTGGGGGCGGTCGCAGTTGCAGCGGTCCGGGTAGCCGGCGTTGCTGGGGGCt                                                                          <  2:3446188/146‑1 (MQ=255)

                                                               cGACTCGCCCGGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGGCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCt                                                                          <  2:2710536/151‑1 (MQ=11)

                                                               cGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCTACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCt                                                                          <  1:5541152/151‑1 (MQ=11)

                                                               cGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCt                                                                          >  1:3202146/1‑151 (MQ=11)

                                                               cGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGCTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGGTGCTGGGGGCt                                                                          >  2:2159349/1‑151 (MQ=11)

                                                                gACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCtt                                                                         >  1:1728630/1‑151 (MQ=11)

                                                                  cTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTc                                                                       <  1:3021468/151‑1 (MQ=11)

                                                                   tCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCa                                                                      >  1:4808797/1‑151 (MQ=11)

                                                                    cGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCAc                                                                     <  2:6125399/151‑1 (MQ=11)

                                                                     gCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCAcc                                                                    >  1:1386906/1‑151 (MQ=14)

                                                                      cccAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCggg                                                                                                <  1:2624173/122‑1 (MQ=11)

                                                                      cccAGGGTCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAGAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCt                                                                   <  2:4001369/151‑1 (MQ=11)

                                                                       ccAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCtt                                                                  <  1:3796726/151‑1 (MQ=14)

                                                                           gTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTcagc                                                              >  1:1196463/1‑151 (MQ=14)

                                                                            tGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTcagca                                                             >  2:2964224/1‑151 (MQ=14)

                                                                              cTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGt                                                           >  2:427422/1‑151 (MQ=14)

                                                                               tCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGtt                                                          <  2:5716274/151‑1 (MQ=14)

                                                                                cGACCAGCGGCTTCACATCGGCGCTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGttt                                                         >  1:968939/1‑151 (MQ=14)

                                                                                 gACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTg                                                        >  2:3314816/1‑151 (MQ=14)

                                                                                 gACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTg                                                        >  1:785974/1‑151 (MQ=14)

                                                                                 gACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTg                                                        >  2:2187323/1‑151 (MQ=14)

                                                                                  aCCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGc                                                       >  2:3964334/1‑151 (MQ=14)

                                                                                      gcggcttCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCt                                                   <  2:91870/146‑1 (MQ=14)

                                                                                       cggcttCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCtg                                                  >  1:4203749/5‑151 (MQ=14)

                                                                                         gcttCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACTTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCtgtt                                                >  1:1551867/3‑151 (MQ=14)

                                                                                              acaTCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGa                                           >  1:1721826/1‑151 (MQ=14)

                                                                                               caTCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGaa                                          >  1:4732396/1‑151 (MQ=14)

                                                                                                aTCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAAc                                         <  1:3573757/151‑1 (MQ=14)

                                                                                                 tCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACg                                        <  2:4253965/151‑1 (MQ=14)

                                                                                                  cGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGt                                       <  2:1728630/151‑1 (MQ=14)

                                                                                                  cGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGt                                       <  1:5679669/151‑1 (MQ=14)

                                                                                                   ggCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTc                                      >  2:4211289/1‑151 (MQ=14)

                                                                                                    gcgttCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCGGCTGTTGTTGAACGTcc                                     >  2:6373811/4‑151 (MQ=14)

                                                                                                    gcgttCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTcc                                     >  1:9967/4‑151 (MQ=14)

                                                                                                     cgttCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCa                                    <  1:3117472/149‑1 (MQ=14)

                                                                                                     cgttCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCa                                    >  1:4659327/3‑151 (MQ=14)

                                                                                                      gttCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAg                                   >  1:6169695/2‑151 (MQ=14)

                                                                                                       ttCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGc                                  <  1:1951106/151‑1 (MQ=14)

                                                                                                          aggcTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCGACCTTGGTCAGGGCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGt                               <  2:1202670/148‑1 (MQ=14)

                                                                                                           ggcTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGTg                              <  2:3081058/149‑1 (MQ=14)

                                                                                                            gcTGCCGAACAAAAAGCCGCGGTACGACTCAGAGCGTGCGACCTTGGTCGAGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGTgg                             <  2:6284672/150‑1 (MQ=14)

                                                                                                                  gaccaaaaaGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGTGGAACggg                       <  2:3585809/144‑1 (MQ=255)

                                                                                                                  gaacaaaaaGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGTGGAACggg                       >  1:3343804/8‑151 (MQ=255)

                                                                                                                  gaacaaaaaGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGTGGAACggg                       >  1:221360/8‑151 (MQ=255)

                                                                                                                    acaacaaGCCCCGGTACGACTCAAAACGTGCCACCATGGCCAGGAAGTGGGAGCCGTCGCAGTGGAAGCTGTCGGGGTCGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTCTTGAACGTCCAGCCCTGGGACGGTCa                     >  2:1526217/6‑151 (MQ=255)

                                                                                                                      aaaaaGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGGCTTTCACCTTCAGCAGTTTGGCGCTGTTGGTGGAAGGTCAGCCGTGGAACGGGCAgg                   >  2:656555/4‑151 (MQ=14)

                                                                                                                       aaaaGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGTGGAACGGGCAGGt                  >  1:4897445/3‑151 (MQ=255)

                                                                                                                       aaaaGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGTGGAACGGGCAGGt                  <  2:2135412/149‑1 (MQ=255)

                                                                                                                        aacgCCCCGGTACGACTCAAAGCGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGTGGAACGGGCAGGta                 <  1:918994/148‑1 (MQ=255)

                                                                                                                         aaGCGCCGGTACGACTCAACACGGGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCTCCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGTGGAACGGGCAGGtat                <  2:847546/151‑1 (MQ=14)

                                                                                                                           gCCGCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGTGGAACGGGCAGGTATAg              <  2:3091327/151‑1 (MQ=255)

                                                                                                                           gCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGTGGAACGGGCAGGTATAg              >  2:6166480/1‑151 (MQ=255)

                                                                                                                            ccccGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGTGGAACGGGCAGGTATAGc             >  1:4511231/1‑151 (MQ=255)

                                                                                                                             cccGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGTGGAACGGGCAGGTATAGCt            >  2:5993433/1‑151 (MQ=255)

                                                                                                                              ccGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGTGGAACGGGCAGGTATAGCTg           >  1:3487400/1‑151 (MQ=255)

                                                                                                                               cGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGTGGAACGGGCAGGTATAGCTgg          <  2:1430992/151‑1 (MQ=255)

                                                                                                                                   aCGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGTGGAACGGGCAGGTATAGCTGGAACg      <  1:3447485/151‑1 (MQ=255)

                                                                                                                                   aCGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGTGGAACGGGCAGGTATAGCTGGAACg      >  1:5741701/1‑151 (MQ=255)

                                                                                                                                      aCTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGTGGAACGGGCAGGTATAGCTGGAACGGtt   <  2:2950263/151‑1 (MQ=255)

                                                                                                                                       cTCGAACCGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGTGGAACGGGCAGGTATAGCTGGAACGGTTg  <  2:5887431/151‑1 (MQ=255)

GGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGTGGAACGGGCAGGTATAGCTGGAACGGTTG  >  CP009974/5105264‑5105549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`