BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	5,513,866	G→A	A266V (GCA→GTA)	RPPX_24600 ←	integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	5,513,866	0	G	A	99.6%	921.2 / NA	279	A266V (GCA→GTA)	RPPX_24600	integrase
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (140/136); total (142/137)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 279 total aligned reads displayed.

GTCCTGGTTCGAGACTTATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTGCAGCCTGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGAAACGCTTCAGGTAGAAAAACTCAGA  >  CP009974/5513718‑5514008

gTCCTGGTTCGAGACTTATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACa                                                                                                                                              <  2:1393326/151‑1 (MQ=255)

 tCCTGGTTCGAGACTTATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAg                                                                                                                                             <  1:6503028/151‑1 (MQ=255)

  ccTGGTTGGAGACTTATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAGCCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGc                                                                                                                                            <  2:1655805/151‑1 (MQ=255)

  ccTGGTTCGAGACTTATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGc                                                                                                                                            <  2:335730/151‑1 (MQ=255)

   cTGGTTCGAGACTTATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGcc                                                                                                                                           >  2:4065726/1‑151 (MQ=255)

   cTGGTTCGAGACTTATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGcc                                                                                                                                           <  1:3225256/151‑1 (MQ=255)

    tGGTTCGAGACTTATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCCAAAGTTGGA‑CAGTTCTAAGCTACAGccc                                                                                                                                          >  1:1235572/1‑150 (MQ=255)

    tGGTTCGAGACTTATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGccc                                                                                                                                          <  1:5206584/151‑2 (MQ=255)

        tCGAGACTTATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGc                                                                                                                                      >  1:1489153/1‑151 (MQ=255)

         cGAGACTTATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGcc                                                                                                                                     >  1:4420245/1‑151 (MQ=255)

          gagaCTTATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGccc                                                                                                                                    >  1:5540885/1‑151 (MQ=255)

          gagaCTTATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGccc                                                                                                                                    >  2:3600713/1‑151 (MQ=255)

           agaCTTATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGCGCCCt                                                                                                                                   >  2:2480007/1‑151 (MQ=255)

              cTTATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGtt                                                                                                                                >  2:4159056/1‑150 (MQ=255)

              cTTATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTa                                                                                                                                <  2:1108840/151‑1 (MQ=255)

              cTTATGGCTAAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTa                                                                                                                                <  1:2271187/151‑1 (MQ=255)

                 aTGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGCTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTc                                                                                                                             >  2:4816034/1‑151 (MQ=255)

                   ggCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTAtt                                                                                                                              <  1:1242711/148‑1 (MQ=255)

                    gCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCcac                                                                                                                          >  2:759427/1‑151 (MQ=255)

                      tCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAg                                                                                                                        <  2:4273437/151‑1 (MQ=255)

                       caggcaggAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAgg                                                                                                                       >  1:568435/1‑151 (MQ=255)

                            aggAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCa                                                                                                                  <  1:3773546/151‑1 (MQ=255)

                             ggAGGGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAg                                                                                                                 <  2:5517128/151‑1 (MQ=255)

                               aGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGc                                                                                                               <  1:2186500/151‑1 (MQ=255)

                                gtcgCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGcc                                                                                                              <  2:4570232/148‑1 (MQ=255)

                                gTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGcc                                                                                                              >  1:2443449/1‑151 (MQ=255)

                                 tgcgaGGCGAGTATCGAGACGCGTGAATGAACGGGAGCCGCCTTGCGGCCCTTCGCGGGCCCGCCCCCGCTCACACCTACTCGACTGCCCCACAAGACTTGCACCCGATC‑AATCCACAGCCCGCGCGCTTTATGCCACGGGGCTCAGGCCa                                                                                                             <  2:4883597/147‑1 (MQ=255)

                                 tGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCa                                                                                                             <  2:2281949/151‑1 (MQ=255)

                                 tGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCa                                                                                                             >  1:1061727/1‑151 (MQ=255)

                                  ggCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCAt                                                                                                            <  2:6338969/151‑1 (MQ=255)

                                   gCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCCCGCGCCCACAGCTTGGACGGCCCCCCAAAAGTTGGA‑CAGGTCTAAGCTTCAGCCCGATCCCTGTATTCCACATGGCTCAGGCCCtt                                                                                                           >  2:2698260/1‑151 (MQ=21)

                                    cAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCCAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCAAAGGGCTCAGGCCAATg                                                                                                          >  1:4527605/1‑151 (MQ=255)

                                    cAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCt                                                                                                                    >  2:3373370/1‑141 (MQ=255)

                                      ggCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAg                                                                                                        >  1:3325748/1‑151 (MQ=255)

                                      ggCGGGTATCAATACGATGGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAg                                                                                                        <  2:1722575/151‑1 (MQ=255)

                                       gCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGt                                                                                                       >  1:5614719/1‑151 (MQ=255)

                                          ggTATCAATAGGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGtctc                                                                                                    <  2:5025766/151‑1 (MQ=255)

                                          ggTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTACACAGGGCTCAGGCCATTGAGtctc                                                                                                    >  1:2914040/1‑151 (MQ=255)

                                           gTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGtctcc                                                                                                   >  2:4708099/1‑150 (MQ=255)

                                            tATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAg                                                                                                  >  1:3404321/1‑151 (MQ=255)

                                             aTCAATACGATTGAATGAACCGGGGCGGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGc                                                                                                 <  2:4527238/151‑1 (MQ=255)

                                              tCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCt                                                                                                <  1:107388/151‑1 (MQ=255)

                                               cAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCtt                                                                                               <  1:258511/151‑1 (MQ=255)

                                                aaTACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTg                                                                                              >  1:2901240/1‑151 (MQ=255)

                                                 aTACGATTGACTGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCCCACTCGCTCCCACCATTTGGACTGCCCCCCAAAAGTTGGA‑CACTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGTCTCAGGCCATCGAGCCTCAGCTCGa                                                                                             >  2:585018/1‑151 (MQ=18)

                                                 aTACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGa                                                                                             >  1:4883572/1‑151 (MQ=255)

                                                  tACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGAt                                                                                            >  1:6223425/1‑151 (MQ=255)

                                                  tACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGAt                                                                                            >  2:6072465/1‑151 (MQ=255)

                                                   aCGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATg                                                                                           >  1:5820012/1‑151 (MQ=255)

                                                   aCGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATg                                                                                           <  1:3047957/151‑1 (MQ=255)

                                                     gATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCg                                                                                         <  1:465725/151‑1 (MQ=255)

                                                      cttGAATGGGCCGGGGGCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCCGCCACAATTTGGACGGCCCGCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCGATTGAGTCTCAGCTTGATGCGa                                                                                        <  2:6264830/150‑1 (MQ=18)

                                                           atgaaCCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGCCTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATg                                                                                   <  2:663951/151‑1 (MQ=255)

                                                              aaCCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGtt                                                                                >  1:2223036/1‑151 (MQ=255)

                                                               aCCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTg                                                                               >  1:6078976/1‑151 (MQ=255)

                                                                ccGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTgt                                                                              <  1:1676191/151‑1 (MQ=255)

                                                                 cGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTgta                                                                             >  1:1977380/1‑151 (MQ=255)

                                                                  ggggCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTgtag                                                                            <  1:4156841/151‑1 (MQ=255)

                                                                  ggggCCGCCGTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTgtag                                                                            <  2:314916/151‑1 (MQ=255)

                                                                       cgccTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTg                                                                       <  2:5983836/151‑1 (MQ=255)

                                                                        gccTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGa                                                                      <  1:3877768/151‑1 (MQ=255)

                                                                             gCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTa                                                                 >  2:2120837/1‑151 (MQ=255)

                                                                                  ccTTCGCGGGCTCGCCATCGCTCCTACAATTTGGCATGCCACCCAAAAGTTGGA‑CAGTTCTAAGCTACAGACCGAGCACTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCAt                                                            <  2:577659/151‑1 (MQ=17)

                                                                                      cgcgGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAg                                                        <  2:4096761/151‑1 (MQ=255)

                                                                                       gcgGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGa                                                       >  2:1510095/1‑151 (MQ=255)

                                                                                       gcgGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGa                                                       <  1:6287052/151‑1 (MQ=255)

                                                                                        cgGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGAc                                                      <  2:3526661/151‑1 (MQ=255)

                                                                                         gggCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGAcc                                                     >  1:5331514/1‑151 (MQ=255)

                                                                                             tCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGct                                                 <  1:346771/151‑1 (MQ=255)

                                                                                             tCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGct                                                 <  1:5969545/151‑1 (MQ=255)

                                                                                              cGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGctc                                                >  2:3001616/1‑151 (MQ=255)

                                                                                                cccTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCtctc                                              >  1:161464/1‑151 (MQ=255)

                                                                                                 ccTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGGATGTACTCATCCTGACCAGTtctcc                                             >  1:4095766/1‑150 (MQ=14)

                                                                                                 ccTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATCGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCa                                             <  1:4332424/151‑1 (MQ=255)

                                                                                                    cGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTATTCTTCCAGACTCGCTTTCAgtt                                          >  2:5253533/1‑149 (MQ=14)

                                                                                                    cGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCt                                          <  1:564035/151‑1 (MQ=255)

                                                                                                     gCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCATACCCGCTCTCAGCtc                                         >  1:86453/1‑151 (MQ=255)

                                                                                                        cccACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCtcatc                                      >  1:746834/1‑151 (MQ=255)

                                                                                                         ccACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCtcatca                                     >  2:2895305/1‑151 (MQ=255)

                                                                                                         ccACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCtcatca                                     >  2:1194094/1‑151 (MQ=255)

                                                                                                          cacaATTTGGACTGCCCCCCCAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCaa                                    >  2:2698995/1‑151 (MQ=255)

                                                                                                           acaATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAAt                                   <  1:5775890/151‑1 (MQ=255)

                                                                                                           acaATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAAt                                   <  2:1536459/151‑1 (MQ=255)

                                                                                                            caATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATg                                  >  1:5473306/1‑151 (MQ=255)

                                                                                                              aTTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGct                                <  2:5414578/151‑1 (MQ=18)

                                                                                                                 tGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCtctc                             <  2:2190596/151‑1 (MQ=12)

                                                                                                                  ggACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCg                            <  2:4421413/151‑1 (MQ=9)

                                                                                                                   gACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGa                           <  1:2143473/151‑1 (MQ=14)

                                                                                                                   gACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGa                           <  1:6136419/151‑1 (MQ=9)

                                                                                                                    aCTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGctct                              >  1:4564595/1‑147 (MQ=12)

                                                                                                                      tGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGAAAc                        >  2:6510408/1‑151 (MQ=9)

                                                                                                                         cccccAAAAGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGAAACGCt                     <  2:234340/151‑1 (MQ=14)

                                                                                                                                aaGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGAAACGCTTCAGGTa              <  1:4912150/151‑1 (MQ=9)

                                                                                                                                 aGTTGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGCCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGAAACGCTTCAGGTAg             <  2:1889268/151‑1 (MQ=9)

                                                                                                                                   ttGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGAAACGCTTCAGGTAGaa           >  1:5280554/1‑151 (MQ=12)

                                                                                                                                   ttGGA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGAAACGCTTCAGGTAGaa           >  1:4395950/1‑151 (MQ=12)

                                                                                                                                     ggA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGAACCGCTTCACGTAGaaaa         >  1:5164470/1‑151 (MQ=9)

                                                                                                                                      gA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTGGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCGGCTCAGCAATGCTCTCGAAACGCTTCATGTAGaaaaa        >  2:5123588/1‑151 (MQ=11)

                                                                                                                                      gA‑CAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTAAGCTTGATGCGATCATGGCTGTAGTAGTGAATGTACTCATCAAGACCCGCTCTCAGCCCATCCATGCTCTCGAAACGCTTACGGTAGacaaa        >  2:5910939/1‑151 (MQ=11)

                                                                                                                                         aaGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGAAACGCTTCAGGTAGAAAAACt      <  2:202212/150‑1 (MQ=14)

GTCCTGGTTCGAGACTTATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGA‑CAGTTCTAAGCTGCAGCCTGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGAAACGCTTCAGGTAGAAAAACTCAGA  >  CP009974/5513718‑5514008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`