BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	1,050,398	G→A	A205T (GCC→ACC)	RPPX_04625 →	fumarate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	1,050,398	0	G	A	98.8%	1251.2 / NA	341	A205T (GCC→ACC)	RPPX_04625	fumarate hydratase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (183/153); minor base C (2/2); total (185/156)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 341 total aligned reads displayed.

AAACAAGTCGAAGATGGCCATGCTCAACCCATCCGACTCGATCGTCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGGCCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCAACCAGCTGGGCATCGGCGCCCAGGGCCTGGGTGGCCTGACCACCG  >  CP009974/1050250‑1050545

  aCAAGTCGAAGATGGCCATGCTCAACCCATCCGACTCGATCGTCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCaa                                                                                                                                                 <  1:6162988/151‑1 (MQ=255)

  aCAAGTCGAAGATGGCCATGCTCAACCCATCCGACTCGATCGTCGAATGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGAGGTACCGCCGAGAAGGCCGCGGTCATGACCaa                                                                                                                                                 >  1:765039/1‑151 (MQ=255)

   cAAGGCGAAGATGGCCATGCTCAACCCATCCGACTCGATCGGCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAg                                                                                                                                                <  1:6105220/151‑1 (MQ=255)

    aaGTCGAAGATGGCCATGCTCAACCCATCCGACTCGATCGTCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAgg                                                                                                                                               >  2:6056433/1‑151 (MQ=255)

         gAAGATGGCCATGCTCAACCCCTCCGACTCGATCGTCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAgtg                                                                                                                                          <  2:4179112/151‑1 (MQ=255)

         gAAGATGGCCATGCTCAACCCATCCGACTCGATCGTCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCAAGACCAAGGAAgtg                                                                                                                                          >  2:1179819/1‑151 (MQ=255)

          aaGATGGCCATGCTCAACCCATCCGACTCGATCGTCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAgtgt                                                                                                                                         <  2:1780884/151‑1 (MQ=255)

           aGATGGCCATGCTCAACCCATCCGACTCGATCGTCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGtt                                                                                                                                        >  2:1703928/1‑151 (MQ=255)

             aTGACCATGCTAAACCCACCCGCCACGAACGTCGACTGGGTACTGAAAACCATGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCACCGGCGGTACCGCCGAGAAGGCCGAGGTCATGACCAAGGAAGTGTTGa                                                                                                                                      <  2:2951915/151‑1 (MQ=255)

               ggCCATGCTCAACCCATCCGACTCGATCGTCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATg                                                                                                                                    >  2:3640229/1‑151 (MQ=255)

               ggCCATGCTCAACCCATCCGACTCGATCGTCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATg                                                                                                                                    >  2:2352815/1‑151 (MQ=255)

                  cATGCTCAACCCATCCGACTCGATCGTCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAg                                                                                                                                 <  2:4782392/151‑1 (MQ=255)

                   aTGCTCAACCCATCCGACTCGATCGTCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGt                                                                                                                                <  2:629547/151‑1 (MQ=255)

                   aTGCTCAACCCATCCGACTCGATCGTCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGt                                                                                                                                >  1:1862970/1‑151 (MQ=255)

                     gCTCAACCCATCCGACTCGATCGTCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTcc                                                                                                                              >  1:5266844/1‑151 (MQ=255)

                      cTCAACCCATCCGACTCGATCGTCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCa                                                                                                                             <  2:2749640/151‑1 (MQ=255)

                      cTCAACCCATCCGACTCGATCGTCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCa                                                                                                                             <  1:2821473/151‑1 (MQ=255)

                       tCAACCCATCCGACTCGATCGTCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAggtcat                                                                                                                            >  1:4193958/1‑146 (MQ=255)

                        cAACCCATCCGACTCGATCGTCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATc                                                                                                                           <  1:188024/151‑1 (MQ=255)

                          aCCCATCCGACTCGATCGTCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAg                                                                                                                                                <  1:6452011/128‑1 (MQ=255)

                             cATCCGACTCGATCGTCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACAt                                                                                                                      <  1:1102438/151‑1 (MQ=255)

                              aTCCGACTCGATCGTCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATc                                                                                                                     >  1:276784/1‑151 (MQ=255)

                              aTCCGACTCGATCGTCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATc                                                                                                                     >  2:5698545/1‑151 (MQ=255)

                                ccGACTCGATCGTCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCa                                                                                                                   >  1:297185/1‑151 (MQ=255)

                                    cTCGATCGTCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGCGCTGATGGAGCCCATCGCCATCCACGaa                                                                                                               >  2:4380782/1‑151 (MQ=255)

                                    cTCGATCGTCGACGGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGaa                                                                                                               <  1:3066688/151‑1 (MQ=255)

                                     tCGATCGTCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAAc                                                                                                              >  1:5791270/1‑151 (MQ=255)

                                      cGATCGTCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACt                                                                                                             <  1:1728999/151‑1 (MQ=255)

                                       gATCGTCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTg                                                                                                            >  1:6506450/1‑151 (MQ=255)

                                        aTCGTCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGa                                                                                                           >  1:1476795/1‑151 (MQ=255)

                                          cgtcgACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAg                                                                                                         <  1:4692021/151‑1 (MQ=255)

                                          cgtcgACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAg                                                                                                         >  1:1025587/1‑151 (MQ=255)

                                           gtcgACTGGGTACTGAAAACCGTGCCGGCCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAgg                                                                                                        <  1:44036/151‑1 (MQ=255)

                                           gtcgACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGaatg                                                                                                        >  1:266040/1‑149 (MQ=255)

                                            tcgACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGc                                                                                                       >  1:1642392/1‑151 (MQ=255)

                                              gACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGccc                                                                                                     <  1:1577338/151‑1 (MQ=255)

                                               aCTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCg                                                                                                    <  2:5791270/151‑1 (MQ=255)

                                               aCTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCg                                                                                                    >  1:5737419/1‑151 (MQ=255)

                                                   ggTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGc                                                                                                <  2:6153356/151‑1 (MQ=255)

                                                    gTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGcc                                                                                               >  1:5613150/1‑151 (MQ=255)

                                                     tACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGccc                                                                                              <  1:2031319/151‑1 (MQ=255)

                                                       cTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCcac                                                                                            <  2:6506450/151‑1 (MQ=255)

                                                          aaaaCCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGa                                                                                         <  1:4550886/151‑1 (MQ=255)

                                                            aaCCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAAc                                                                                       >  1:4480981/1‑151 (MQ=255)

                                                            aaCCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAAc                                                                                       >  2:1275250/1‑151 (MQ=255)

                                                            aaCCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAAc                                                                                       <  1:5172451/151‑1 (MQ=255)

                                                            aaCCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAAc                                                                                       <  1:3991439/151‑1 (MQ=255)

                                                             aCCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAAcc                                                                                      <  1:3749241/151‑1 (MQ=255)

                                                              ccGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCg                                                                                     <  2:1993235/151‑1 (MQ=255)

                                                                gTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGcc                                                                                   >  1:3276593/1‑151 (MQ=255)

                                                                 tGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCt                                                                                  >  1:2564263/1‑151 (MQ=255)

                                                                 tGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCt                                                                                  <  1:1330865/151‑1 (MQ=255)

                                                                   ccGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTgg                                                                                <  1:3432352/151‑1 (MQ=255)

                                                                   ccGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTgg                                                                                <  1:5226992/151‑1 (MQ=255)

                                                                     gACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTggca                                                                              >  1:799690/1‑149 (MQ=255)

                                                                      aCCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGgaag                                                                             <  2:1898061/151‑1 (MQ=255)

                                                                            ggTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATcc                                                                       <  1:5132825/151‑1 (MQ=255)

                                                                             gTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCg                                                                      <  1:778731/151‑1 (MQ=255)

                                                                             gTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCg                                                                      <  1:431055/151‑1 (MQ=255)

                                                                                  ggctggTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACAGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTgg                                                                 >  1:4929604/1‑151 (MQ=255)

                                                                                   gctggTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGa                                                                >  1:4729843/1‑151 (MQ=255)

                                                                                    ctggTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCAGAGAACCGCCTGGAAGAAATCCGCCTGGAg                                                               <  2:3852187/151‑1 (MQ=255)

                                                                                     tggTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGc                                                              >  2:1060433/1‑151 (MQ=255)

                                                                                       gTGCCCACCTGGCGTGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTg                                                            <  1:6300603/151‑1 (MQ=255)

                                                                                        tGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGt                                                           <  2:3496815/151‑1 (MQ=255)

                                                                                        tGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGt                                                           <  2:2197685/151‑1 (MQ=255)

                                                                                          cccACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTc                                                         <  2:3411427/151‑1 (MQ=255)

                                                                                           ccACCTGGCATGCTCGGCATCGGCATCCGCGGTCCCGCCGAGACGGCCGCGGTCATGACGAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGGCCGTGGCCCACACAACCGCCCGGAACAAATCCGCCCGGCGCTGTTCg                                                        >  2:3098483/1‑151 (MQ=255)

                                                                                             aCCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGAc                                                      >  1:941366/1‑151 (MQ=255)

                                                                                                tGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAg                                                   <  2:6536515/151‑1 (MQ=255)

                                                                                                   cATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTc                                                >  1:5408054/1‑151 (MQ=255)

                                                                                                    aTGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCa                                               <  1:3614248/151‑1 (MQ=255)

                                                                                                        tcggcatcggcaTCGGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCAACCa                                           <  1:1427113/151‑1 (MQ=255)

                                                                                                           gcatcggcatcgGCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGCCACGGTCCACCAGCt                                        >  2:4129260/1‑151 (MQ=255)

                                                                                                            catcggcatcggCGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCAACCAGCTg                                       >  2:1434269/1‑151 (MQ=255)

                                                                                                                ggcatcggcGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTg                                                                  >  1:1695035/1‑120 (MQ=255)

                                                                                                                 gcagcggcGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCAACCAGCTGGGCAt                                  <  2:5084522/151‑1 (MQ=255)

                                                                                                                 gcacccgcggTACCGCCGAGAAGGCCGCGGTCATGACCAAGAAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCTCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCAACCAGCTGGGCAt                                  <  1:1213773/145‑1 (MQ=255)

                                                                                                                   atcggcGGTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCAACCAGCTGGGCATCg                                <  2:804464/151‑1 (MQ=255)

                                                                                                                        cggTACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCAACCAGCTGGGCATCGGCGcc                           >  1:1282294/1‑151 (MQ=255)

                                                                                                                           tACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCAACCAGCTGGGCATCGGCGCCCAg                        >  2:2397811/1‑151 (MQ=255)

                                                                                                                           tACCGCCGAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCAACCAGCTGGGCATCGGCGCCCAg                        >  2:2237051/1‑151 (MQ=255)

                                                                                                                               gccgAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCAACCAGCTGGGCATCGGCGCCCAGGGcc                    >  1:4814892/1‑151 (MQ=255)

                                                                                                                                ccgAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCAACCAGCTGGGCATCGGCGCCCAGGGCCt                   >  1:3566450/1‑151 (MQ=255)

                                                                                                                                ccgAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCAACCAGCTGGGCATCGGCGCCCAGGGCCt                   >  2:1671758/1‑151 (MQ=255)

                                                                                                                                 cgAGAAGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCAACCAGCTGGGCATCGGCGCCCAGGGCCTg                  <  1:2220401/151‑1 (MQ=255)

                                                                                                                                 cgAGAAGGCCGCGGGCATGACGAAGGAAGTGGTGATGGAGTCCATCGACAGCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCAACCAGCTGGGCATCGGCGCCCAGGCCCTg                  <  2:5392106/151‑1 (MQ=255)

                                                                                                                                     gaGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCGCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCAACCAGCTGGGCATCGGCGCCCAGGGCCTGGGTg              <  2:2446201/150‑1 (MQ=255)

                                                                                                                                     aaGGCCGCGGTGATGACCGAGGAAGTGTTGATGGAGTCCATCGACATCCCGGCACTGAAGGCCCGTGGCCCACAGAGCCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCAACCAGCTGGGCATCGGCGCCCAGGGCCTGGGTg              <  2:5934591/151‑1 (MQ=255)

                                                                                                                                     aaGGCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCAACCAGCTGGGCATCGGCGCCCAGGGCCTGGGTg              <  2:4823724/151‑1 (MQ=255)

                                                                                                                                       ggCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCAACCAGCTGGGCATCGGCGCCCAGGGCCTGGGTGGc            >  1:581316/1‑151 (MQ=255)

                                                                                                                                       ggCCGCGGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCAACCAGCTGGGCATCGGCGCCCAGGGCCTCGGTGGc            >  2:5655656/1‑151 (MQ=255)

                                                                                                                                          cgcgGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCAACCAGCTGGGCATCGGCGCCCAGGGCCTGGGTGGCCTg         >  2:1809936/1‑151 (MQ=255)

                                                                                                                                            gggTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCAACCAGCTGGGCATCGGCGCCCAGGGCCTGGGTGGCCTGac       <  1:5344733/150‑1 (MQ=255)

                                                                                                                                            cgGTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCAACCAGCTGGGCATCGGCGCCCAGGGCCTGGGTGGCCTGac       >  2:750487/1‑151 (MQ=255)

                                                                                                                                             ggTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCAACCAGCTGGGCATCGGCGCCCAGGGCCTGGGTGGCCTGacc      <  2:474076/151‑1 (MQ=255)

                                                                                                                                              gTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCAACCAGCTGGGCATCGGCGCCCAGGGCCTGGGTGGCCTGacca     <  2:6360405/151‑1 (MQ=255)

                                                                                                                                              gTCATGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCAACCAGCTGGGCATCGGCGCCCAGGGCCTGGGTGGCCTGacca     <  2:4252012/151‑1 (MQ=255)

                                                                                                                                                 aTGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCAACCAGCTGGGCATCGGCGCCCAGGGCCTGGGTGGCCTGACCACCg  >  1:2459520/1‑151 (MQ=255)

                                                                                                                                                 aTGACCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCAACCAGCTGGGCATCGGCGCCCAGGGCCTGGGTGGCCTGACCACCg  >  2:39151/1‑151 (MQ=255)

AAACAAGTCGAAGATGGCCATGCTCAACCCATCCGACTCGATCGTCGACTGGGTACTGAAAACCGTGCCGACCATGGGTGCTGGCTGGTGCCCACCTGGCATGCTCGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCGCGGTCATGGCCAAGGAAGTGTTGATGGAGTCCATCGACATCCACGAACTGAAGGCCCGTGGCCCACAGAACCGCCTGGAAGAAATCCGCCTGGAGCTGTTCGACAAGGTCAACCAGCTGGGCATCGGCGCCCAGGGCCTGGGTGGCCTGACCACCG  >  CP009974/1050250‑1050545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`