Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	1,154,311	T→C	T337T (ACT→ACC)	RPPX_05115 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	1,154,311	0	T	C	100.0%	30.7 / NA	17	T337T (ACT→ACC)	RPPX_05115	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/7);  total (10/7)

GTTCAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACTGACCTGGCCCT  >  CP009974/1154164‑1154322                                                                                                                                                    |            gTTCAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACcgaccgac          >  1:4037106/1‑151 (MQ=25) gTTCAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACcgaccgac          >  2:3595361/1‑151 (MQ=25) gTTCAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACcgaccgac          >  2:2365730/1‑151 (MQ=25)  ttCAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTCCAGCCCCTGAACAACCgaccgacc         >  2:4127730/1‑151 (MQ=25)  ttCAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCgaccgacc         >  1:352403/1‑151 (MQ=25)   tCAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcct        >  1:2326420/1‑151 (MQ=21)    cAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACGTCACGGCACCGGACGTCGACACCCCTGATACTACATCCCCTGAACAAACGACCGAcctg       >  2:6234793/1‑151 (MQ=255)    cAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctg       <  1:2594441/151‑1 (MQ=18)    cAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctg       >  2:5522587/1‑151 (MQ=18)    cAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctg       <  2:1310033/151‑1 (MQ=18)    cAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctg       >  2:2916973/1‑151 (MQ=18)     aGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctgg      >  2:728865/1‑151 (MQ=17)     aGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctgg      >  1:139443/1‑151 (MQ=17)      gCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctggc     <  1:714179/151‑1 (MQ=14)      gCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctggc     >  2:5671255/1‑151 (MQ=14)        aTTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctggccc   <  1:5991257/151‑1 (MQ=14)        aTTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctggccc   >  1:5965405/1‑151 (MQ=14)        aTTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctggccc   >  2:6537186/1‑151 (MQ=14)        aTTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctggccc   >  1:3208927/1‑151 (MQ=14)        aTTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGGTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctggccc   <  2:4157575/151‑1 (MQ=14)         ttACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctggccct  <  2:1408737/151‑1 (MQ=11)         ttACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctggccct  <  1:4362525/151‑1 (MQ=11)                                                                                                                                                    |            GTTCAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACTGACCTGGCCCT  >  CP009974/1154164‑1154322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑