Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 1,174,412 A→G D407G (GAT→GGT)  RPPX_05135 → ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099741,174,4120AG99.7% 1104.8 / NA 309D407G (GAT→GGT) RPPX_05135ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (151/157);  minor base C (1/0);  total (152/157)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 309 total aligned reads displayed.

TCCAGGGCCTGGACGAGATCAAGGCACTGCAGGCAGAGGCACGCTTCGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGATGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGGCGCCGCTGGCCCAGCTGACCCATGTGCTGACCCGCTGGCA  >  CP009974/1174265‑1174559
                                                                                                                                                   |                                                                                                                                                   
tCCAGGGCGTGGACGAGATCAAGGCACTGCAGGCAGAGGCACGCTTCGAAGGACAGTGGAGCCAGTACAACGCGGCCTGCGCGCGCACCACCCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGc                                                                                                                                                  <  1:1505689/151‑1 (MQ=255)
 ccAGGGCCTGGACGAGATCAAGGCACTGCAGGCAGAGGCACGCTTCGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGcc                                                                                                                                                 >  2:2330473/1‑151 (MQ=255)
        cTGGACGAGATCAAGGCACTGCAGGCAGAGGCACGCTTCGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTg                                                                                                                                          >  1:5758756/1‑151 (MQ=255)
         tGGACGAGATCAAGGCACTGCAGGCAGAGGCACGCTTCGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGc                                                                                                                                         <  2:3621609/151‑1 (MQ=255)
         cggACGAGAACACGGCACTGCAGGCCGAGGCGCGCTTGGAACGCGCGTGGAACCAGTAGAGCGCGGCGGGCGCGGGCACCGACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGc                                                                                                                                         <  2:2510582/150‑1 (MQ=255)
          ggACGAGATCAAGGCACTGCAGGCAGAGGCACGCTTCGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGcc                                                                                                                                        >  2:183633/1‑151 (MQ=255)
          ggACGAGATCAAGGCACTGCAGGCAGAGGCACGCTTCGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGcc                                                                                                                                        >  2:3507755/1‑151 (MQ=255)
            gcGAGATGAGGGCACTGGGGGCAGAGGCACGCTTCGAACGACAGTGGAACCAGGACAGCGCGGCCGGCGCGCACACCACCCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGt                                                                                                                                      <  2:2916734/150‑1 (MQ=255)
            aCGAGATCAAGGCACTGCAGGCAGAGGCACGCTTCGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGt                                                                                                                                      >  1:5014435/1‑151 (MQ=255)
             cGAGATCAAGGCACTGCAGGCAGAGGCACGCTTCGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTg                                                                                                                                     <  1:3295781/151‑1 (MQ=255)
              gagaTCAAGGCACTGCAGGCAGAGGCACGCTTCGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTgg                                                                                                                                    >  1:6311679/1‑151 (MQ=255)
              gagaTCAAGGCACTGCAGGCAGAGGCACGCTTCGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTgg                                                                                                                                    >  2:2698870/1‑151 (MQ=255)
               agaTCAAGGCACTGCAGGCAGAGGCACGCTTCGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGt                                                                                                                                   <  2:1167530/151‑1 (MQ=255)
                  tCAAGGCACTGCAGGCAGAGGCACGCTTCGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCAt                                                                                                                                >  2:3402266/1‑151 (MQ=255)
                    aaGGCACTGCAGGCAGAGGCACGCTTCGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGt                                                                                                                                      >  2:1714562/1‑143 (MQ=255)
                    aaGGCACTGCAGGCAGAGGCACGCTTCGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGt                                                                                                                                      <  1:1714562/143‑1 (MQ=255)
                      ggCACTGCAGGCAGAGGCACGCTTCGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTg                                                                                                                            <  2:2584236/151‑1 (MQ=255)
                      ggCACTGCAGGCAGAGGCACGCTTCGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTg                                                                                                                            <  1:3722550/151‑1 (MQ=255)
                          cTGCAGGCAGAGGCACGCTTCGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCg                                                                                                                        <  1:3289110/151‑1 (MQ=255)
                          cTGCAGGCAGAGGCACGCTTCGAACGACAGTGGAACCAGTACAACGCGGCCAGCGCGCACACCAACCCGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGCGTTTGCCGCGGCCATCGTGATCg                                                                                                                        >  2:705094/1‑151 (MQ=255)
                              aggcagAGGCACGCTTCGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCCGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGCGGTCATCGTGATCCgcgc                                                                                                                    >  2:6543626/1‑151 (MQ=255)
                                gcagAGGCACGCTTCGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGccc                                                                                                                  >  2:4535242/1‑151 (MQ=255)
                                gcagAGGCACGCTTCGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGCGGTCCTCGTGCTCGGCGccc                                                                                                                  >  2:3973940/1‑151 (MQ=255)
                                  agagGCACGCTTCGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGccccc                                                                                                                <  1:6494578/151‑1 (MQ=255)
                                  agagGCACGCTTCGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGccccc                                                                                                                >  1:1603997/1‑151 (MQ=255)
                                   gagGCACGCTTCGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCa                                                                                                               <  1:3355363/151‑1 (MQ=255)
                                     ggCACGCTTCGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATg                                                                                                             <  1:5703937/151‑1 (MQ=255)
                                          gCTTCGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCAt                                                                                                        <  1:2558743/151‑1 (MQ=255)
                                          gCTTCGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCAt                                                                                                        <  1:3675947/151‑1 (MQ=255)
                                             tCGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGc                                                                                                     >  1:6449016/1‑151 (MQ=255)
                                             gcGAGCGACAGTGGCAGCAGTACAGCGCGGCCGGCGCGCGCACCGACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGc                                                                                                     <  2:6411525/150‑1 (MQ=255)
                                              cGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGcc                                                                                                    >  2:2745877/1‑151 (MQ=255)
                                                aaCGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCgg                                                                                                  <  1:1073654/151‑1 (MQ=255)
                                                   gACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGGGGGCATCGTGATCGGCGCCCCCCTGGTCATCGccgccgc                                                                                               >  2:4271615/1‑150 (MQ=255)
                                                      aGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCt                                                                                            <  1:2299185/151‑1 (MQ=255)
                                                       gTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTc                                                                                           >  1:3207349/1‑151 (MQ=255)
                                                          gAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTcacc                                                                                        <  1:614516/151‑1 (MQ=255)
                                                            aCCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTcaccac                                                                                      <  2:6159096/151‑1 (MQ=255)
                                                              cAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACgg                                                                                    >  2:5370335/1‑151 (MQ=255)
                                                               aGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACggg                                                                                   <  1:2704759/151‑1 (MQ=255)
                                                               aGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCAATGGTCATCGCCGGCGACCTCACCACggg                                                                                   <  1:6526812/151‑1 (MQ=255)
                                                                  aCATCGCGGGCTGCGCGCACACCGACCTGCGCGTGCGCACCCTCGCCGACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAg                                                                                <  2:3770643/151‑1 (MQ=255)
                                                                  aCAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAg                                                                                <  1:1304963/151‑1 (MQ=255)
                                                                   cAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGc                                                                               <  2:1437587/151‑1 (MQ=255)
                                                                   cAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGCGGTTGCCGGGCCCCTCGTGATCGGCGCCCCCATGGTCGCCGCCGGCGACCCCACCACgaggccc                                                                               >  2:3274622/1‑145 (MQ=255)
                                                                      cgcgGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCtg                                                                            <  1:6066000/151‑1 (MQ=255)
                                                                        gggCCGGCGCGGACACGACCCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCtggt                                                                          <  2:4687336/150‑1 (MQ=255)
                                                                        cgGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTGACCAACGGCCTGGTGACCTGGACGCAGAACGTGCCGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCtggt                                                                          <  2:5129994/151‑1 (MQ=255)
                                                                         ggCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCtggtg                                                                         >  1:2092529/1‑151 (MQ=255)
                                                                            cTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTggcg                                                                      <  2:631318/151‑1 (MQ=255)
                                                                             tGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTggcgg                                                                     <  1:4257842/151‑1 (MQ=255)
                                                                             tGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGCGCCGTGTTTGCCGGGGTCATCGTGATCGGCGCCCCCATCGTCATCGCCGGCGACCTCACCACGGGGAGCCCGGGgccgg                                                                     >  2:4139156/1‑151 (MQ=255)
                                                                               cgcgcACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCACCGCCCGCGACCTCACCACGGGTAACCTGGGGGCGGCa                                                                   >  1:6182257/1‑151 (MQ=255)
                                                                                  gcACACCACCCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGAGGCATcc                                                                <  2:6127909/151‑1 (MQ=255)
                                                                                   cacaCCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCa                                                               >  1:29126/1‑151 (MQ=255)
                                                                                     cacCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCCTg                                                             >  1:4664502/1‑151 (MQ=255)
                                                                                       ccAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCt                                                           <  1:309104/151‑1 (MQ=255)
                                                                                       ccAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCt                                                           >  1:5248152/1‑151 (MQ=255)
                                                                                         aaCCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCttt                                                         <  1:455672/151‑1 (MQ=255)
                                                                                           cctgcgcctgcgCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCa                                                       >  2:449262/1‑151 (MQ=255)
                                                                                            ctgcgcctgcgcACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCAt                                                      >  2:5773512/1‑151 (MQ=255)
                                                                                            ctgcgcctgcgcACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCAt                                                      <  1:3597218/151‑1 (MQ=255)
                                                                                              gcgcctgcgcACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCAGGTGGCGGCATCCATGCTTTCATcg                                                    <  2:6033180/151‑1 (MQ=255)
                                                                                                gcctgcgcACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATcgcg                                                  >  1:910199/1‑151 (MQ=255)
                                                                                                gcctgcgcACACTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATcgcg                                                  <  1:5512059/151‑1 (MQ=255)
                                                                                                  ctgcgcACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCCTCGTGATCGGCGCCCCCATGGTCATCGCCCGCGACCTCACCACGCGCAGCCTGCTGGCGGCATCCATGCCCTCACCCCGga                                                >  2:6345313/1‑151 (MQ=255)
                                                                                                   tgcgcACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGgat                                               >  1:5866641/1‑151 (MQ=255)
                                                                                                    gcgcACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGgatg                                              <  2:217392/151‑1 (MQ=255)
                                                                                                    gcgcACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGgatg                                              >  1:5402044/1‑151 (MQ=255)
                                                                                                     cgcACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGgatga                                             >  2:2103568/1‑151 (MQ=255)
                                                                                                       cACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGatgatg                                           <  2:131912/151‑1 (MQ=255)
                                                                                                          ccTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGgcg                                        <  1:2214879/151‑1 (MQ=255)
                                                                                                           cTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGgcgg                                       <  2:1003556/151‑2 (MQ=255)
                                                                                                           cTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGgcgc                                       <  1:607766/151‑1 (MQ=255)
                                                                                                           cTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGgcgc                                       <  2:1394067/151‑1 (MQ=255)
                                                                                                            tCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGGcgcc                                      <  1:442337/151‑1 (MQ=255)
                                                                                                            tCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGGcgcc                                      <  1:2004166/151‑1 (MQ=255)
                                                                                                            tCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGGcgcc                                      >  2:3202076/1‑151 (MQ=255)
                                                                                                              aCCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGGcgccgc                                    <  1:3173022/151‑1 (MQ=255)
                                                                                                                cAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGGCGCCGCTg                                  <  1:4772222/151‑1 (MQ=255)
                                                                                                                cAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGGCGCCGCTg                                  >  1:1026619/1‑151 (MQ=255)
                                                                                                                 aaCGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGGCGCCGCTgg                                 >  1:4113331/1‑151 (MQ=255)
                                                                                                                   cGGCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGGCGCCGCTGGcc                               >  2:1743542/1‑151 (MQ=255)
                                                                                                                    ggCCTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGGCGCCGCTGGccc                              <  2:823512/151‑1 (MQ=255)
                                                                                                                       cTGGTGACCTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGGCGCCGCTGGCCCAGc                           <  1:2408/151‑1 (MQ=255)
                                                                                                                              ccTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGGCGCCGCTGGCCCAGCTGACCCa                    <  2:2631534/151‑1 (MQ=255)
                                                                                                                               cTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGGCGCCGCTGGCCCAGCTGACCCAt                   <  1:4829944/151‑1 (MQ=255)
                                                                                                                               cTGGACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGGCGCCGCTGCCCCACCTGACCCAt                   >  2:1828170/1‑151 (MQ=255)
                                                                                                                                 ggACGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGGCGCCGCTGGCCCAGCTGACCCAtgt                 >  1:3971082/1‑151 (MQ=255)
                                                                                                                                   aCGCAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGCCCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGGCGCCGCTGGCCCAGCTGACCCATGTGc               <  2:5502860/151‑1 (MQ=255)
                                                                                                                                      cAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGGCGCCGCTGGCCCAGCTGACCCATGTGCTGa            <  1:544049/151‑1 (MQ=255)
                                                                                                                                      cAGAACGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGGCGCCGCTGGCCCAGCTGACCCATGTGCTGa            <  2:1198125/151‑1 (MQ=255)
                                                                                                                                         aaCGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGGCGCCGCTGGCCCAGCTGACCCATGTGCTGAccc         <  2:3863134/151‑1 (MQ=255)
                                                                                                                                         aaCGTGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGGCGCCGCTGGCCCAGCTGACCCATGTGCTGAccc         <  2:2261226/151‑1 (MQ=255)
                                                                                                                                             tGCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGGCGCCGCTGGCCCAGCTGACCCATGTGCTGACCCGCTg     >  2:4421232/1‑151 (MQ=255)
                                                                                                                                              gCAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGGCGCCGCTGGCCCAGCTGACCCATGTGCTGACCCGCTgg    >  1:191615/1‑151 (MQ=255)
                                                                                                                                               cAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGGCGCCGCTGGCCCAGCTGACCCATGTGCTGACCCGCTGgc   >  1:2816588/1‑151 (MQ=255)
                                                                                                                                               cAGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGGCGCCGCTGGCCCAGCTGACCCATGTGCTGACCCGCTGgc   <  2:754718/151‑1 (MQ=255)
                                                                                                                                                aGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGGCGCCGCTGGCCCAGCTGACCCATGTGCTGACCCGCTGgcc  >  2:281107/1‑150 (MQ=255)
                                                                                                                                                aGGGTGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGGCGCCGCTGGCCCAGCTGACCCATGTGCTGACCCGCTGgca  <  2:1646816/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TCCAGGGCCTGGACGAGATCAAGGCACTGCAGGCAGAGGCACGCTTCGAACGACAGTGGAACCAGTACAACGCGGCCTGCGCGCACACCAACCTGCGCCTGCGCACCCTCACCAACGGCCTGGTGACCTGGACGCAGAACGTGCAGGATGCCGTGTTTGCCGTGGTCATCGTGATCGGCGCCCCCATGGTCATCGCCGGCGACCTCACCACGGGTAGCCTGGTGGCGGCATCCATGCTTTCATCGCGGATGATGGCGCCGCTGGCCCAGCTGACCCATGTGCTGACCCGCTGGCA  >  CP009974/1174265‑1174559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑