BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	1,554,729	C→A	G226G (GGG→GGT)	tuf ←	elongation factor Tu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	1,554,729	0	C	A	99.7%	887.5 / NA	295	G226G (GGG→GGT)	tuf	elongation factor Tu
Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (153/141); minor base G (0/1); total (153/142)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 297 total aligned reads displayed.

GCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTACAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGCCCCGAGATCGAGAATACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGC  >  CP009974/1554582‑1554875

gCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGaccc                                                                                                                                                 <  1:3061739/151‑1 (MQ=17)

   aGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCgag                                                                                                                                              >  1:1737996/1‑151 (MQ=17)

    gCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCgaga                                                                                                                                             <  2:506234/151‑1 (MQ=12)

       cGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCg                                                                                                                                          >  1:2068033/1‑151 (MQ=12)

            ttCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGaac                                                                                                                                     <  1:5505353/151‑2 (MQ=12)

             tCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGGTCGATACGACCGGTAACAACGGTACCACGACCCGAGCTCGAGaaca                                                                                                                                    >  2:5722770/1‑149 (MQ=255)

                tCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGt                                                                                                                                 >  1:2940658/1‑151 (MQ=255)

                  cagcagCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGCGATCGAGAACACGTCt                                                                                                                               >  2:5540913/1‑151 (MQ=255)

                    gcagcTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTc                                                                                                                             >  2:4113264/1‑151 (MQ=255)

                     cagcTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCg                                                                                                                            >  1:6135229/1‑151 (MQ=12)

                      agcTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCg                                                                                                                            <  1:10922/150‑1 (MQ=255)

                      agcTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGa                                                                                                                           >  1:6408076/1‑151 (MQ=255)

                       gcTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGAt                                                                                                                          >  1:4240406/1‑151 (MQ=255)

                        cTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGAtt                                                                                                                         <  2:610350/151‑1 (MQ=9)

                          tGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTgg                                                                                                                       >  1:5144009/1‑151 (MQ=255)

                             ggAACATCTCAACGCCGGTGTAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGATCGCGGACGATACCACGCTCGATACGCCCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCAt                                                                                                                    <  1:3943582/151‑1 (MQ=11)

                                  aTCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGa                                                                                                               >  2:2020981/1‑151 (MQ=9)

                                   tctcAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGaa                                                                                                              >  1:3767009/1‑151 (MQ=12)

                                    ctcAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATc                                                                                                                   >  2:3417722/1‑145 (MQ=255)

                                      cAACGCCGGTGCAGGTGATGGTGGTGGTGTCACGCAGACCAACGATCTCCAGCGGATCCTGAACGCGGACGATACCACGCCCGATACGACCGGTAACAACGGTACCAGGACCCGAGGTCGAGAACACGTCTTCGATTGGCATCAGGAACgg                                                                                                           <  2:4172891/151‑1 (MQ=11)

                                       aaCACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGCACACGTCTTCGATTGGCACCAGCAACGGc                                                                                                          >  1:3174056/1‑151 (MQ=11)

                                       aaCACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGc                                                                                                          <  2:4791889/151‑1 (MQ=12)

                                        acacCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCt                                                                                                         <  1:4569621/151‑1 (MQ=255)

                                          acCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTgg                                                                                                       <  2:680294/151‑1 (MQ=255)

                                              gtgcAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGa                                                                                                   >  1:115214/4‑151 (MQ=12)

                                               tgcAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGAt                                                                                                  <  2:326757/149‑1 (MQ=9)

                                               ggcAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGAt                                                                                                  <  2:2398054/149‑1 (MQ=12)

                                                gcAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATg                                                                                                 <  2:2075243/150‑1 (MQ=9)

                                                 cAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATgg                                                                                                <  2:5622638/151‑1 (MQ=12)

                                                  aGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGc                                                                                               <  1:3417048/151‑1 (MQ=12)

                                                  aGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGc                                                                                               >  1:5125932/1‑151 (MQ=12)

                                                     tggtggtggtggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCCCg                                                                                            >  2:5981292/1‑151 (MQ=9)

                                                       gtggtggtggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGaa                                                                                          <  1:3123878/151‑1 (MQ=17)

                                                       gtggtggtggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGaa                                                                                          <  1:5323773/151‑1 (MQ=12)

                                                         ggtggtggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACt                                                                                        >  1:1813262/1‑151 (MQ=12)

                                                           tggtggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTgg                                                                                      >  2:461502/1‑151 (MQ=12)

                                                              tggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTc                                                                                   <  1:4822075/151‑1 (MQ=12)

                                                              tggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTc                                                                                   <  2:2826663/151‑1 (MQ=14)

                                                                gtgtCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAg                                                                                 <  1:5042055/151‑1 (MQ=12)

                                                                    cACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGAt                                                                             >  2:1198442/1‑151 (MQ=21)

                                                                    cACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGACGATGGCACGACCTGGCTCAGGGaa                                                                             >  2:521090/1‑150 (MQ=255)

                                                                     aCGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGCTg                                                                            >  2:4572913/1‑151 (MQ=16)

                                                                         aGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAgg                                                                        >  1:4098624/1‑151 (MQ=255)

                                                                              aaCGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATcc                                                                   <  2:3133353/151‑1 (MQ=255)

                                                                                cGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCag                                                                 <  2:1609321/151‑1 (MQ=255)

                                                                                   tttCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCagagg                                                              >  1:6219462/1‑150 (MQ=255)

                                                                                   tttCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGt                                                              <  2:857421/151‑1 (MQ=255)

                                                                                   tttCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGt                                                              <  1:4743054/151‑1 (MQ=255)

                                                                                    ttCCAGCGGATCCTTAACGCGGACGATCCCACTCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGtt                                                             <  1:5642063/151‑1 (MQ=255)

                                                                                    ttCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGtt                                                             <  2:764412/151‑1 (MQ=255)

                                                                                      ccAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCACCCAGAGTTTc                                                           >  2:4975933/1‑151 (MQ=255)

                                                                                       cAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCTGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCt                                                          <  2:6305272/151‑1 (MQ=255)

                                                                                          cGGATCCTGACCGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTAcc                                                       <  2:5779420/151‑1 (MQ=255)

                                                                                          cGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTAcc                                                       <  2:3890482/151‑1 (MQ=255)

                                                                                           ggATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCa                                                      <  1:881186/151‑1 (MQ=255)

                                                                                           ggATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCa                                                      >  1:260879/1‑151 (MQ=255)

                                                                                           ggATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCa                                                      >  1:923568/1‑151 (MQ=255)

                                                                                                gtGAACGCGGACGATCCCACGCTGGAGACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGcttc                                                 <  2:3506565/150‑1 (MQ=14)

                                                                                                 tGAACGCGGACGGTACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGcttct                                                <  2:3790314/151‑1 (MQ=255)

                                                                                                 tGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGcttct                                                <  2:2101987/151‑1 (MQ=255)

                                                                                                  gAACGGCGACGCTACCGCGCTCGGTACGACCGGTAACAGCGGTACCACGGCCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGcttctt                                               <  2:4434102/151‑1 (MQ=14)

                                                                                                  gAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGcttctt                                               >  1:662621/1‑151 (MQ=255)

                                                                                                   aaCGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGc                                                    >  1:1542769/1‑145 (MQ=255)

                                                                                                    aCGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGa                                             <  1:3482402/151‑1 (MQ=255)

                                                                                                     cgcgGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGAc                                            <  2:534676/151‑1 (MQ=255)

                                                                                                      gcgGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACa                                           <  1:5835383/151‑1 (MQ=255)

                                                                                                         gACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCg                                        >  1:1236303/1‑151 (MQ=255)

                                                                                                         gACGATACCACGCTCGATACGACCGGTAAAAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCg                                        <  2:2913195/151‑1 (MQ=255)

                                                                                                           cGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGt                                      <  2:270844/151‑1 (MQ=255)

                                                                                                              tACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGt                                   >  1:1358975/1‑151 (MQ=255)

                                                                                                               aCCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTg                                  >  1:2227127/1‑151 (MQ=255)

                                                                                                               aCCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTg                                  >  1:5242210/1‑151 (MQ=255)

                                                                                                                 cACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGcc                                >  1:1972647/1‑151 (MQ=255)

                                                                                                                  aCGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGccc                               >  1:6064008/1‑151 (MQ=255)

                                                                                                                    gCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCAt                             <  2:2510940/151‑1 (MQ=255)

                                                                                                                      tCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCAttt                           <  1:3616347/151‑1 (MQ=255)

                                                                                                                       cGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTc                          <  1:6099014/151‑1 (MQ=255)

                                                                                                                        gATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCg                         <  1:2831303/151‑1 (MQ=255)

                                                                                                                        gATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCg                         >  1:3701845/1‑151 (MQ=255)

                                                                                                                         aTACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGt                        >  1:6258373/1‑151 (MQ=255)

                                                                                                                          taagACCGGTAACAACGGTACCACGAACCGAGATCGAGAACACGTCTTCGATTGGCATCAGGACCGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGtt                       <  2:1108784/148‑1 (MQ=255)

                                                                                                                          tACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGtt                       <  1:2013126/151‑1 (MQ=255)

                                                                                                                           aCGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTg                      >  1:5417945/1‑151 (MQ=255)

                                                                                                                           aCGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTg                      >  2:6212930/1‑151 (MQ=255)

                                                                                                                           aCGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTg                      >  1:1770174/1‑151 (MQ=255)

                                                                                                                             gACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTgtc                    >  2:1496304/1‑151 (MQ=255)

                                                                                                                              aCCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTgtcg                   <  2:3727340/151‑1 (MQ=255)

                                                                                                                                 ggTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCt                >  1:3900647/1‑151 (MQ=255)

                                                                                                                                   tAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCttt              <  2:3941444/151‑1 (MQ=255)

                                                                                                                                   tAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCttt              <  1:1689172/151‑1 (MQ=255)

                                                                                                                                    aacaacGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTg             >  2:1716518/1‑151 (MQ=255)

                                                                                                                                      caacGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGcc           >  1:2315220/1‑151 (MQ=255)

                                                                                                                                      caacGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGcc           <  2:1968370/151‑1 (MQ=255)

                                                                                                                                       aacGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCt          >  2:6054281/1‑151 (MQ=255)

                                                                                                                                       aacGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCt          <  1:5926035/151‑1 (MQ=255)

                                                                                                                                         cGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGCTGTAGGCATCCAGAGTTTCTACCAGCCTCTTGACCGCGGTCGTGCCTGTATCGTTGTCGTCCTTGccctt        >  2:5329852/1‑148 (MQ=14)

                                                                                                                                          ggTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTcc       <  1:2493321/151‑1 (MQ=255)

                                                                                                                                           gTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCa      <  2:2068033/151‑1 (MQ=255)

                                                                                                                                               cACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAgcgc  >  1:4517269/1‑151 (MQ=255)

                                                                                                                                               cACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGc  >  2:6395607/1‑151 (MQ=255)

GCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTACAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGCCCCGAGATCGAGAATACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGC  >  CP009974/1554582‑1554875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`