BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	1,554,744	T→C	V221V (GTA→GTG)	tuf ←	elongation factor Tu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	1,554,744	0	T	C	99.7%	942.6 / NA	303	V221V (GTA→GTG)	tuf	elongation factor Tu
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (162/140); minor base G (0/1); total (162/141)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 303 total aligned reads displayed.

TCCAGCAGCTTGCGGAACATCTCAACACCGGTACAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGCCCCGAGATCGAGAATACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGAACCG  >  CP009974/1554598‑1554891

tCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGt                                                                                                                                                 <  1:3127766/151‑1 (MQ=12)

tCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGt                                                                                                                                                 >  1:2940658/1‑151 (MQ=255)

  cagcagCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGCGATCGAGAACACGTCt                                                                                                                                               >  2:5540913/1‑151 (MQ=255)

   agcagcTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCtt                                                                                                                                              <  2:2048978/151‑1 (MQ=255)

     cagcTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCg                                                                                                                                            >  1:1992646/1‑151 (MQ=12)

     cagcTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCg                                                                                                                                            >  1:6135229/1‑151 (MQ=12)

     cagcTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCg                                                                                                                                            >  2:4693120/1‑151 (MQ=9)

      agcTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCg                                                                                                                                            <  1:10922/150‑1 (MQ=255)

      agcTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGa                                                                                                                                           >  1:1778435/1‑151 (MQ=255)

       gcTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGAt                                                                                                                                          >  1:4240406/1‑151 (MQ=255)

            cGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCa                                                                                                                                     <  1:5152352/151‑1 (MQ=255)

             ggAACATCTCAACGCCGGTGTAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGATCGCGGACGATACCACGCTCGATACGCCCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCAt                                                                                                                                    <  1:3943582/151‑1 (MQ=11)

                   tctcAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGaa                                                                                                                              >  1:3767009/1‑151 (MQ=12)

                    ctcAGCACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCACCGATTTCCGGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAAc                                                                                                                             <  2:5698216/151‑1 (MQ=11)

                    ctcAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATc                                                                                                                                   <  1:3417722/145‑1 (MQ=12)

                       aaCACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGc                                                                                                                          >  2:6042449/1‑151 (MQ=9)

                              gtgcAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGa                                                                                                                   >  1:1191825/4‑151 (MQ=12)

                              gtgcAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCAAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGa                                                                                                                   >  2:5037870/4‑151 (MQ=255)

                               ggcAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGAt                                                                                                                  <  2:6542307/149‑1 (MQ=12)

                                 cAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATgg                                                                                                                >  2:321455/1‑151 (MQ=12)

                                     tggtggtggtggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCCCg                                                                                                            >  2:5981292/1‑151 (MQ=9)

                                     tggtggtggtggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACg                                                                                                            >  2:4451844/1‑151 (MQ=12)

                                     tggtggtggtggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACg                                                                                                            >  2:845392/1‑151 (MQ=12)

                                           tggtggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTgg                                                                                                      >  2:2014604/1‑151 (MQ=12)

                                              tggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTc                                                                                                   <  2:2826663/151‑1 (MQ=14)

                                              tggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTc                                                                                                   <  1:4822075/151‑1 (MQ=12)

                                              tggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTc                                                                                                   >  2:6234264/1‑151 (MQ=12)

                                                gtgtCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAg                                                                                                 <  1:6466953/151‑1 (MQ=17)

                                                    cACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGAt                                                                                             >  2:1198442/1‑151 (MQ=21)

                                                     aCGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGCTCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGACCTCGCCCAGGGCTg                                                                                            >  2:4573014/1‑151 (MQ=11)

                                                       gCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTa                                                                                          <  1:4536493/151‑1 (MQ=255)

                                                         aGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAgg                                                                                        >  1:4098624/1‑151 (MQ=255)

                                                             cAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATc                                                                                    >  1:5486674/1‑151 (MQ=255)

                                                              aaCGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATcc                                                                                   <  2:3133353/151‑1 (MQ=255)

                                                               aCGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCa                                                                                  >  1:3187234/1‑151 (MQ=255)

                                                                cGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCag                                                                                 <  2:1609321/151‑1 (MQ=255)

                                                                  aTTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCagag                                                                               <  1:5364756/151‑1 (MQ=255)

                                                                   tttCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCagagg                                                                              >  1:6219462/1‑150 (MQ=255)

                                                                   tttCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGt                                                                              <  1:4742101/151‑1 (MQ=255)

                                                                    ttCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGtt                                                                             <  2:764412/151‑1 (MQ=255)

                                                                        aGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTa                                                                         >  1:145943/1‑151 (MQ=255)

                                                                         gCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTAc                                                                        >  1:787016/1‑151 (MQ=255)

                                                                          gggATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTAcc                                                                       <  2:4157698/150‑1 (MQ=255)

                                                                          cGGATCCTGACCGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTAcc                                                                       <  2:5779420/151‑1 (MQ=255)

                                                                          cGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTAcc                                                                       <  2:6135229/151‑1 (MQ=255)

                                                                          cGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTAcc                                                                       <  2:3890482/151‑1 (MQ=255)

                                                                           ggATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCa                                                                      >  1:260879/1‑151 (MQ=255)

                                                                           ggATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCa                                                                      >  1:923568/1‑151 (MQ=255)

                                                                           ggATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCa                                                                      <  1:881186/151‑1 (MQ=255)

                                                                                 tGAACGCGGACGGTACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGcttct                                                                <  2:3790314/151‑1 (MQ=255)

                                                                                 tGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGcttct                                                                <  2:2101987/151‑1 (MQ=255)

                                                                                    aCGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGa                                                             <  1:3482402/151‑1 (MQ=255)

                                                                                     cgcgGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGAc                                                            <  2:534676/151‑1 (MQ=255)

                                                                                     cgcgGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGAc                                                            <  1:4451844/151‑1 (MQ=255)

                                                                                        ggACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGc                                                         <  2:2074375/151‑1 (MQ=255)

                                                                                          aCGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCgg                                                       >  1:5070105/1‑151 (MQ=255)

                                                                                           cGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGt                                                      <  2:270844/151‑1 (MQ=255)

                                                                                            gATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTa                                                     >  1:5136940/1‑151 (MQ=255)

                                                                                              tACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTCGCTCAGCGATGTAGACATCCACAGCTCCTACCAGCTACTTGACAGCGGTact                                                   >  2:4078772/1‑149 (MQ=11)

                                                                                                 cACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGcc                                                >  1:1733301/1‑151 (MQ=255)

                                                                                                  aCGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGccc                                               >  1:6064008/1‑151 (MQ=255)

                                                                                                    gCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCAt                                             <  1:5252359/151‑1 (MQ=255)

                                                                                                    gCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCAt                                             <  2:2510940/151‑1 (MQ=255)

                                                                                                     cTCGATACGACCGGTAACAACGGTACCACGACCCGATATCGAAAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCAtt                                            <  1:3064312/151‑1 (MQ=255)

                                                                                                     cTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCAtt                                            >  1:1884917/1‑151 (MQ=255)

                                                                                                      tCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCAttt                                           <  1:3616347/151‑1 (MQ=255)

                                                                                                       cGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTc                                          <  1:6099014/151‑1 (MQ=255)

                                                                                                        gATACGACGGGTAACAACGGTACCACGACCCGGGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCg                                         <  2:3335737/151‑1 (MQ=255)

                                                                                                        gATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCg                                         <  1:2831303/151‑1 (MQ=255)

                                                                                                        gATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCg                                         >  1:3701845/1‑151 (MQ=255)

                                                                                                         aTACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGt                                        >  1:6258373/1‑151 (MQ=255)

                                                                                                          taagACCGGTAACAACGGTACCACGAACCGAGATCGAGAACACGTCTTCGATTGGCATCAGGACCGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGtt                                       <  2:1108784/148‑1 (MQ=255)

                                                                                                             gACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTgtc                                    >  2:1496304/1‑151 (MQ=255)

                                                                                                              aCCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTgtcg                                   <  2:3727340/151‑1 (MQ=255)

                                                                                                                cGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTATGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTgtcgtc                                 <  2:6305634/151‑1 (MQ=255)

                                                                                                                 ggTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCt                                >  1:3900647/1‑151 (MQ=255)

                                                                                                                   tAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCttt                              <  2:3941444/151‑1 (MQ=255)

                                                                                                                    aacaacGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTg                             <  2:1865542/151‑1 (MQ=255)

                                                                                                                    aacaacGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTg                             >  2:1716518/1‑151 (MQ=255)

                                                                                                                     acaacGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGc                            >  1:2037395/1‑151 (MQ=255)

                                                                                                                      caacGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGcc                           >  1:2315220/1‑151 (MQ=255)

                                                                                                                       aacGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCt                          <  1:5926035/151‑1 (MQ=255)

                                                                                                                         cGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGCTGTAGGCATCCAGAGTTTCTACCAGCCTCTTGACCGCGGTCGTGCCTGTATCGTTGTCGTCCTTGccctt                        >  2:5329852/1‑148 (MQ=14)

                                                                                                                          ggTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTcc                       >  1:5797036/1‑151 (MQ=255)

                                                                                                                          ggTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTcc                       <  1:2493321/151‑1 (MQ=255)

                                                                                                                            tACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAg                     <  1:1684364/151‑1 (MQ=255)

                                                                                                                               cACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGc                  <  2:3489519/151‑1 (MQ=255)

                                                                                                                                  gacccGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCAt               >  2:4298055/3‑151 (MQ=255)

                                                                                                                                    cccGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATAc             <  1:1259777/151‑1 (MQ=255)

                                                                                                                                      cGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGa           >  2:6073380/1‑151 (MQ=255)

                                                                                                                                       gagaTCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAg          >  1:477348/1‑151 (MQ=255)

                                                                                                                                       gagaTCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAg          >  1:2270651/1‑151 (MQ=255)

                                                                                                                                        agaTCGAGAACACGTCTTCGATTGGCATCAGGCACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGc         <  1:5700461/151‑1 (MQ=255)

                                                                                                                                          aTCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGCTGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCgg       >  2:239773/1‑151 (MQ=255)

                                                                                                                                          aTCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCgg       <  2:3508313/151‑1 (MQ=255)

                                                                                                                                           tCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGa      >  2:2960634/1‑151 (MQ=255)

                                                                                                                                            cGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGaa     >  2:5274569/1‑151 (MQ=255)

                                                                                                                                             gagaACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGAAc    >  2:4850128/1‑151 (MQ=255)

                                                                                                                                              agaACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGAAcc   >  1:2110396/1‑151 (MQ=255)

                                                                                                                                               gaACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGAACCg  <  2:5167561/151‑1 (MQ=255)

TCCAGCAGCTTGCGGAACATCTCAACACCGGTACAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGCCCCGAGATCGAGAATACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGAACCG  >  CP009974/1554598‑1554891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`