BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	1,555,302	A→G	V35V (GTT→GTC)	tuf ←	elongation factor Tu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	1,555,302	0	A	G	99.7%	733.2 / NA	290	V35V (GTT→GTC)	tuf	elongation factor Tu
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (149/140); total (150/140)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 290 total aligned reads displayed.

GACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGATTGCCGAACCGAAAACTTCGGAGCAAACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTAGCCTTGTTTTTT  >  CP009974/1555154‑1555448

 aCCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGGGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGAcg                                                                                                                                                 <  2:433140/151‑1 (MQ=9)

   cTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGAcgcg                                                                                                                                               >  1:3843535/1‑151 (MQ=17)

    tGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGa                                                                                                                                              >  1:4221093/1‑151 (MQ=14)

      ggCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGt                                                                                                                                            >  1:341791/1‑151 (MQ=12)

      ggCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGt                                                                                                                                            <  1:563056/151‑1 (MQ=12)

      ggCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGt                                                                                                                                            >  1:1825511/1‑151 (MQ=12)

        cAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCa                                                                                                                                          <  2:2442164/151‑1 (MQ=12)

        cAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCa                                                                                                                                          >  2:2059056/1‑151 (MQ=12)

         aGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCag                                                                                                                                         <  1:3793871/151‑1 (MQ=12)

          gTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCaga                                                                                                                                        >  2:190542/1‑151 (MQ=17)

            cAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGc                                                                                                                                      >  1:5766136/1‑151 (MQ=12)

             aaCGGGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCt                                                                                                                                     <  2:4888233/151‑1 (MQ=9)

              aCGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTg                                                                                                                                    <  1:366597/151‑1 (MQ=14)

               cGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGc                                                                                                                                   <  2:3275317/151‑1 (MQ=14)

                gTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCg                                                                                                                                  >  2:4197916/1‑151 (MQ=17)

                  gAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGt                                                                                                                                >  1:2022849/1‑151 (MQ=12)

                   aGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTc                                                                                                                               >  2:1841520/1‑151 (MQ=12)

                   aGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTc                                                                                                                               <  1:3207124/151‑1 (MQ=12)

                    gCGTAGTGACGAATGTTCGGGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCa                                                                                                                              <  2:379598/151‑1 (MQ=9)

                    gCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCa                                                                                                                              <  2:3621155/151‑1 (MQ=9)

                     cGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCag                                                                                                                             >  2:4424130/1‑151 (MQ=17)

                         gTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTg                                                                                                                         <  2:3032169/151‑1 (MQ=12)

                          tGACGAATGTTCGAGTTGTACGCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACGCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTgg                                                                                                                        <  2:5926148/151‑1 (MQ=9)

                           gACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTggg                                                                                                                       >  2:5088601/1‑150 (MQ=12)

                           gACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGt                                                                                                                       >  2:4631297/1‑151 (MQ=17)

                             cGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCt                                                                                                                     <  1:3709900/151‑1 (MQ=12)

                               aaTGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTTAGATCTGCGGTTTGAGTgggttta                                                                                                                   >  2:1364629/1‑146 (MQ=11)

                                                    gTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTTTTTTGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGCAAACTTCGGCGCAGACGCGAGCCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCCACGTGGCCGATg                                                                                              >  2:4815727/1‑151 (MQ=11)

                                                    gTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATg                                                                                              >  1:913813/1‑151 (MQ=2)

                                                     tGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATgg                                                                                             >  2:2712214/1‑151 (MQ=9)

                                                     ggggCGGTGTTGGTGGTGATGCCGCGCGCGGTTTCTTCCGGGGCCGAGTCGAGCTTGTCGGACTCAACGGCTGCCGAGGCGAAAACTTCGGAGCAGACGCGAGTCAGGGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATgg                                                                                             <  2:6248151/148‑1 (MQ=11)

                                                      gAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGt                                                                                            <  2:1970259/151‑1 (MQ=9)

                                                       ggCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTg                                                                                           <  2:4055687/150‑1 (MQ=9)

                                                         cGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGcc                                                                                         <  1:3358853/151‑1 (MQ=12)

                                                          ggTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCg                                                                                        >  1:2469716/1‑151 (MQ=12)

                                                             gtTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGTCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACg                                                                                     <  2:3914543/151‑1 (MQ=12)

                                                                gATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTa                                                                                  <  2:146472/151‑1 (MQ=12)

                                                                gATGGTGATACCGCGCGCTTGTTCTTCCGGGGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTa                                                                                  <  2:2211754/151‑1 (MQ=255)

                                                                  tGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAAc                                                                                <  2:5907653/151‑1 (MQ=12)

                                                                  tGGTCATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAAc                                                                                <  1:2392030/151‑1 (MQ=12)

                                                                   ggTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACg                                                                               >  1:4968671/1‑151 (MQ=17)

                                                                   ggTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACg                                                                               <  2:6479269/151‑1 (MQ=12)

                                                                    gTGATACCGCGCGCTTTTTCTTTTTTTTCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGt                                                                              >  2:2522475/1‑151 (MQ=11)

                                                                     tGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTg                                                                             >  1:3616358/1‑151 (MQ=12)

                                                                         aCCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGggga                                                                         >  1:1017613/1‑151 (MQ=14)

                                                                         aCCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTCCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGggga                                                                         >  2:3468397/1‑151 (MQ=9)

                                                                          cggcgcgcTTTTGCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTGCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGgggaa                                                                        <  2:5791929/149‑1 (MQ=255)

                                                                           cgcgcgCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGgggaag                                                                       >  1:1483756/1‑151 (MQ=9)

                                                                            gcgcgcTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGgggaagg                                                                      <  1:4611032/151‑1 (MQ=12)

                                                                             cgcgcTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGgggaaggg                                                                     >  2:2880915/1‑151 (MQ=9)

                                                                               cgcTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGGTAACGTGgggaagggaa                                                                   >  1:6473224/1‑151 (MQ=9)

                                                                               cgcTGTTTCTTCCGGGGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGgggaagggaa                                                                   <  2:6047167/151‑1 (MQ=255)

                                                                                  tttttCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGa                                                                >  1:1913177/1‑151 (MQ=12)

                                                                                  tttttCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGa                                                                >  1:5412905/1‑151 (MQ=14)

                                                                                    tttCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATc                                                              >  1:3477298/1‑151 (MQ=12)

                                                                                    tttCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATc                                                              >  2:4275827/1‑151 (MQ=9)

                                                                                    tttCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATc                                                              >  1:6393373/1‑151 (MQ=12)

                                                                                     ttcttcCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCa                                                             <  1:5173442/151‑1 (MQ=12)

                                                                                      tcttcCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCaa                                                            >  2:4707109/1‑151 (MQ=9)

                                                                                      tcttcCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCaa                                                            <  2:4694149/151‑1 (MQ=12)

                                                                                       cttcCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCaaa                                                           >  1:5474412/1‑151 (MQ=12)

                                                                                         tcCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAAtt                                                         <  1:5131027/151‑1 (MQ=16)

                                                                                          ccGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAAttt                                                        <  2:5766136/151‑1 (MQ=21)

                                                                                              ggCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCt                                                    <  2:5678204/150‑1 (MQ=255)

                                                                                              aGCCGAGTCGATCTTGTCGAACCCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCt                                                    <  1:1065819/151‑1 (MQ=255)

                                                                                                ccGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCttt                                                  >  2:3174153/1‑151 (MQ=255)

                                                                                                ccGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCttt                                                  <  1:3153370/151‑1 (MQ=255)

                                                                                                 ggAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTa                                                 <  2:3567769/150‑1 (MQ=255)

                                                                                                 cGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTa                                                 <  2:233602/151‑1 (MQ=255)

                                                                                                 cGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTa                                                 <  2:800711/151‑1 (MQ=255)

                                                                                                   aGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGc                                               <  1:5086747/151‑1 (MQ=255)

                                                                                                   aGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGCGGGCACGGGCACGATCAAATTTCTCTTTAGc                                               >  2:5604431/1‑151 (MQ=11)

                                                                                                   aGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTCCGGTCAGAGCGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGACCAAATTTTTCTTTAGc                                               >  2:2843046/1‑151 (MQ=11)

                                                                                                     tCGATCTTGTCGAACTCAACGACTGCCGAGCCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCa                                             <  2:2021358/151‑1 (MQ=255)

                                                                                                     tCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCa                                             >  1:5954926/1‑151 (MQ=255)

                                                                                                      cGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCAc                                            <  1:4876780/151‑1 (MQ=255)

                                                                                                       gATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACg                                           <  1:2264782/151‑1 (MQ=255)

                                                                                                       gATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACg                                           >  1:2715928/1‑151 (MQ=255)

                                                                                                        aTCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGa                                          >  1:1440261/1‑151 (MQ=255)

                                                                                                          cTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACa                                        <  2:636432/151‑1 (MQ=255)

                                                                                                           ttGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAg                                       <  2:5226235/151‑1 (MQ=255)

                                                                                                                 aaCTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAAcc                                 <  2:5540197/151‑1 (MQ=255)

                                                                                                                   cTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACctc                               >  2:1626519/1‑151 (MQ=255)

                                                                                                                    tCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACctct                              <  1:2660629/151‑1 (MQ=255)

                                                                                                                     cAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCtt                             <  1:6206576/151‑1 (MQ=255)

                                                                                                                      aaCGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTg                            <  1:826729/151‑1 (MQ=255)

                                                                                                                       aCGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGc                           <  1:3016706/151‑1 (MQ=255)

                                                                                                                        cGACTGCCGAACCGAAAACTGCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGcc                          <  1:2614436/151‑1 (MQ=255)

                                                                                                                          actGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTa                        >  2:2376695/3‑151 (MQ=255)

                                                                                                                          actGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTa                        >  2:5161654/3‑151 (MQ=255)

                                                                                                                             gCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTATCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACATTGAACCTCTTGTCTAAAg                     >  1:1724858/1‑151 (MQ=255)

                                                                                                                             gCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAg                     <  2:1248754/151‑1 (MQ=255)

                                                                                                                              ccgaaccgaaAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAgg                    >  1:2785431/1‑151 (MQ=255)

                                                                                                                              ccgaaccgaaAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAgg                    >  1:2543406/1‑151 (MQ=255)

                                                                                                                               cgaaccgaaAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAggg                   <  1:4411329/151‑1 (MQ=255)

                                                                                                                                   ccgaaAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGAtt               <  1:4662598/151‑1 (MQ=255)

                                                                                                                                     gaaAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTAg             <  2:6085757/151‑1 (MQ=255)

                                                                                                                                     gaaAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTAg             <  2:1113939/151‑1 (MQ=255)

                                                                                                                                      aaaaCTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTAGc            <  2:539529/151‑1 (MQ=255)

                                                                                                                                        aaCTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTAGCCt          >  1:4036922/1‑151 (MQ=255)

GACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGATTGCCGAACCGAAAACTTCGGAGCAAACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTAGCCTTGTTTTTT  >  CP009974/1555154‑1555448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`